Virulence factors and beta-lactamase production among vancomycin-resistant Enterococcus faecalis isolated from clinical samples and hospital environment

  • Authors

    • Moses David Department of Microbiology, Faculty of Science, Ekiti State University, Ado-Ekiti
    • Kennedy Imonitie Department of Microbiology, Faculty of Science, Ekiti State University, Ado-Ekiti
    • Richard Osuntoyinbo Department of Microbiology, Faculty of Science, Ekiti State University, Ado-Ekiti
    • Adetunji Olawale Department of Microbiology, Faculty of Science, Ekiti State University, Ado-Ekiti
    2016-12-09
    https://doi.org/10.14419/ijbr.v5i1.6811
  • Enterococcus Faecalis, Antibiotics, Virulence, Beta-Lactamase, Hospital.
  • Enterococcus faecalis, though opportunistic pathogen has emerged as one of the leading nosocomial pathogens and has been implicated in different human infections. The severity of the infections caused by this organism is largely due to its complex pathogenic process. The objective of this study was to determine the carriage of virulence factors and vanA gene among the strains of vancomycin-resistant E. faecalis isolated from hospitals. Standard methods were used for isolation, antibiotic susceptibility and detection of virulence factors in the isolates. A total of one hundred and twenty three (123) samples were screened out of which 69 (45.70 %) yielded E. faecalis. The highest percentage of the isolates was recovered from the environment followed by the clinical samples. Children surgical ward had the highest occurrence of the test organism followed by male surgical ward. All the isolates were resistant to both amoxycillin/clavulanic acid and ceftazidime, while 98.55%, 89.86% and 53.62% were resistant to ampicillin, cefuroxime and gentamicin respectively. Only twenty seven (39.13%) of the isolates were resistant to the vancomycin. Among the vancomycin-resistant isolates, haemolysin had the highest occurrence (60.29%) followed by caseinase (55.88%). A total of 16 (59.26%) were beta-lactamase positive while 8 (29.63%) out of the isolates (vancomycin-resistant) were non-biofilm former while vanA genes was detected in 9 (33.33%) of the isolates. This study gives an insight to antibiotic resistant pattern of circulating Enterococcus faecalis and also the isolate showed varying patterns of virulent factors.

  • References

    1. [1] Abderrahmen M, Nadia L, Abir B, Amina B. Evaluation of the probiotic properties of Bacillus spp. strains isolated from Tunisian hypersaline environments. African Journal of Microbiology Research. 2014; 8 (4):398-405. https://doi.org/10.5897/AJMR2013.5457.

      [2] Abderrahmen M, Nadia L, Abir B, Amina B. Evaluation of the probiotic properties of Bacillus spp. strains isolated from Tunisian hypersaline environments. African Journal of Microbiology Research. 2014; 8 (4):398-405. https://doi.org/10.5897/AJMR2013.5457.

      [3] Alksne, L. E. and Projan, S. J. (2000). Bacterial virulence as a target for antimicrobial chemotherapy. CurrOpinBiotechnol 11, 625–636. https://doi.org/10.1016/s0958-1669(00)00155-5

      [4] Andrup, L. and Andersen, K. (1999). A comparison of the kinetics of plasmid transfer in the conjugation systems encoded by the F plasmid from Escherichia coli and plasmid pCF10 from Enterococcusfaecalis. Microbiology 145, 2001–2009. https://doi.org/10.1099/13500872-145-8-2001.

      [5] Bethel CD, Boonlayengoor S. Betalactamase test. In: Clinical microbiology procedures handbook. Isenberg H. D. 2nd Edition. ASM Press. 2004; 1:534-536.

      [6] Billström, H., Lund, B., Sullivan, Å. and Nord, C. E.(2008). Virulence and antimicrobial resistance in clinical Enterococcus faecium. International Journal Antimicrobial Agents. 32: 374–377. https://doi.org/10.1016/j.ijantimicag.2008.04.026.

      [7] Busani, L., Del Grosso, M., Paladini, C., Graziani, C., Pantosti, A., Biavasco, F. and Caprioli, A. (2004). Antimicrobial susceptibility of vancomycin-susceptible and -resistant enterococci isolated in Italy from raw meat products, farm animals, and human infections. International Journal Food Microbiology 97: 17–22. https://doi.org/10.1016/j.ijfoodmicro.2004.04.008.

      [8] CLSI Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard—Eleventh Edition. M02-A11 32(1) Replaces M02-A10. 2012; 29 (1).

      [9] Comerlato CB, de Resende MCC, Caierao J, d’Azevedo PA (2013). Presence of virulence factors in Enterococcus faecalis and Enterococcus faeciumsusceptible and resistant to vancomycin. MemInst Oswaldo Cruz. 108(5): 590-595. https://doi.org/10.1590/S0074-02762013000500009.

      [10] Dahlen, G., Samuelsson, W. and Molander, A. (2000). Identification and antimicrobial susceptibility of enterococci isolated from root canal. Oral MicrobiolImmunol 15, 309–312. https://doi.org/10.1034/j.1399-302x.2000.150507.x.

      [11] Gülhan, T.; Aksakal, A.; Ekin, I.H.; Savașan, S.; and Boynukara, B. 2006. Virulence factors of Enterococcus faeciumand Enterococcus faecalis strains isolated from humans and pets. Turkish Journal of Veterinary and Animal Sciences 30(5): 477-82.

      [12] Holt, J. G., Krieg, N. R., Sneath, P. H. A., Staley, J. T. and Williams, S. T. (1994). Bergey’s Manual of Determinative Bacteriology 9th Edn. Williams and Wilkins, Baltimore.

      [13] Kacmaz, B. and Aksoy, A. (2005). Antimicrobial resistance of enterococci in Turkey. International Journal Antimicrobial Agents 25, 535–538. https://doi.org/10.1016/j.ijantimicag.2005.02.020.

      [14] Kayaoglu, G. and Orstavik, D. (2004). Virulence factors of Enterococcus faecalis: relationship to endodontic disease. Crit Rev Oral Biol Med 15, 308–320. https://doi.org/10.1177/154411130401500506.

      [15] Klare, I., Konstabel, C., Badstubner, D., Werner, G. and Witte, W. (2003). Occurrence and spread of antibiotic resistances in Enterococcus faecium. International Journal Food Microbiology 88, 269–290. https://doi.org/10.1016/S0168-1605(03)00190-9.

      [16] Landman D, Mobaraki NK, Quale JM. (1993). Novel antibiotic regimens against Enterococcus faeciumresistant to ampicillin, vancomycin, and gentamicin. Antimicrob Agents Chemother. 37:1904-8. https://doi.org/10.1128/AAC.37.9.1904.

      [17] Lindenstrau AG, Pavlovic M, Bringmann A, Behr J, Ehrmann MA, Vogel RF 2011. Comparison of genotypic and phenotypic cluster analyses of virulence determinants and possible role of CRISPR elements towards their incidence in Enterococcus faecalis and Enterococcus faecium. SystApplMicrobiol 34: 553-560. https://doi.org/10.1016/j.syapm.2011.05.002.

      [18] Livornese LL, Dias S, Samuel C, Romanowski B, Taylor S, May P (1992). Hospital-acquired infection with vancomycin resistant Enterococcus faeciumtransmitted by electronic thermometers. Ann Intern Med; 117:112-6. https://doi.org/10.7326/0003-4819-117-2-112.

      [19] Lopes, M., Simoes, A. P., Tenreiro, R., Figueiredo Marques, J. J. and Barreto Crespo, M. T. (2006). Activity and expression of a virulence factor, gelatinase, in dairy enterococci. International Journal Food Microbiology 112, 208–214. https://doi.org/10.1016/j.ijfoodmicro.2006.09.004.

      [20] Mannu, L., Paba, A., Daga, E., Comunian, R., Zanetti, S., Dupre, I. and Sechi, L. A. (2003). Comparison of the incidence of virulence determinants and antibiotic resistance between Enterococcus faecium strains of dairy, animal and clinical origin. International Journal Food Microbiology. 88: 291–304. https://doi.org/10.1016/S0168-1605(03)00191-0.

      [21] Moellering RC. Emergence of Enterococcus as a significant pathogen. Clin Infect Dis 1992: 14:1173-8. https://doi.org/10.1093/clinids/14.6.1173.

      [22] Noskin, G. A. (1997). Vancomycin-resistant enterococci: Clinical, microbiologic, and epidemiologic features.Journal of Laboratory and Clinical Medicine. 130(1): 14-20. https://doi.org/10.1016/S0022-2143(97)90054-8.

      [23] Sharma S, Bhat GK, Shenoy S. Virulence factors and drug resistance in Escherichia coli isolated from extra-intestinal infections. Indian J Med Microbiol 2007; 25:369-73. https://doi.org/10.4103/0255-0857.37341.

      [24] Siegfried, L., Kmetova, M., Puzová, H., Molokacova, M., Filka J. (1994) Virulence associated factors in Escherichia coli strains isolated from children with urinary tract infections. Journal of Medical Microbiology. 41: 127-132. https://doi.org/10.1099/00222615-41-2-127.

      [25] Slaughter S, Hayden MK, Nathan C (1996). A comparison of the effect of universal use of gloves and gowns with that of glove use alone on acquisition of vancomycin-resistant enterococci in a medical intensive care unit. Ann. Intern. Med. 215:448-56. https://doi.org/10.7326/0003-4819-125-6-199609150-00004.

      [26] Su YA, Sulavik MC, He P, Makinen KK, Makinen PL, Fiedler S, Wirth R, Clewell DB. Nucleotide sequence of the gelatinase gene (gelE) from Enterococcusfaecalis subs. liquefaciens. Infection and Immunity. 1991; 59:415–420.

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    David, M., Imonitie, K., Osuntoyinbo, R., & Olawale, A. (2016). Virulence factors and beta-lactamase production among vancomycin-resistant Enterococcus faecalis isolated from clinical samples and hospital environment. International Journal of Biological Research, 5(1), 1-5. https://doi.org/10.14419/ijbr.v5i1.6811