Proximate composition, mineral content and amino acid profile of Irvingia gabonensis O’Rorke baill leaf

  • Authors

    • Efosa Ewere University of Uyo, Uyo, Akwa Ibom State, Nigeria.
    • Oboso Etim University of Uyo, Uyo, Akwa Ibom State, Nigeria.
    • Usunomena Usunobun Benson Idahosa University, Benin City, Edo State, Nigeria.
    2016-12-24
    https://doi.org/10.14419/ijsw.v5i1.6969
  • Amino Acid, Irvingia gabonensis O’Rorke Baill, Minerals, Nutritional, Proximate.
  • Several plants are utilized for medicinal and nutritional purposes. Irvingia gabonensis O’Rorke Baill leaf is used in herbal medicine for treatment of a number of ailments. This study was therefore carried out to investigate the proximate composition, antinutritional factors, mineral composition and amino acid profile of Irvingia gabonensis O’Rorke Baill leaf. The proximate and antinutritional factors analyses were done using standard procedures. The mineral analyses were done using flame photometry, titrimetic method, molybdo vanadate method and atomic absorption spectrophotometry and the amino acid profile was done with the aid of Applied Biosystems PTH amino acid analyzer. Results of proximate analyses were carbohydrates (75.15±1.29 %), protein (11.43±1.07 %), fat (1.99±0.74 %), fibre (4.89±0.61 %), ash (6.71±0.28 %), moisture (5.12±0.03 %) and caloric value (364.30±5.95 Kcal). Antinutrients (phytate, oxalate and cyanide) levels in the leaf were also very low. Results from mineral analyses obtained revealed that the leaf is also a very rich source of calcium, potassium, sodium, magnesium and so on. Compared with the World health organization (WHO) standards, results of the amino acid profile showed that the leaf is very rich in isoleucine, leucine, lysine, phenylalanine, threonine, valine and tyrosine which are nutritionally essential amino acids. Furthermore, extraction of the leaf using ethanol reduced the levels of these amino acids but not below the recommended WHO standard levels for most of the essential amino acids. Irvingia gabonensis O’Rorke Baill leaf is therefore a potential source of key nutrients.

  • References

    1. [1] Mollet, M., Tiki-Manga, T., Kengue, J.and Tchoundjeu, Z. (1995). The ‘top 10’ species in Cameroon, a survey of farmers’ views on trees. Agroforestry Today, 7: 14–16.

      [2] Franzel, S., Jaenicke, H. and Janssen, W. (1996). Choosing the Right Trees: Setting Priorities for Multi-purpose Tree Improvement. ISNAR Research Report no. 8. International Service for National Agricultural Research, The Hague, the Netherlands.

      [3] Hubert, DJ. Wabo, F.G., Ngameni, B., Ngheguin, TF. Tchoukoua, A., Ambassa, P., Abia, W., Tchana, AN., Giardina, S., Buonocore, D., Vita, FP., Vidari, G., Marzatico, F., Ngadjui, BT. and Moundipa, PF. (2010). “In vitrohepatoprotective and antioxidant activities of the crude ex-tract and isolated compounds from Irvingia gabonensis. Asian Journal of Traditional Medicine. 5(3): 79-88.

      [4] Matos L, Nzikou JM, Matouba E, Pandzou-Yembe VN, Mapepoulou TG, Linder M, Desobry S. (2009). Studies of Irvingia gabonensis seeds kernels: Oil technological applications. Pakistan. Journal of Nutrition 8: 151-157.

      [5] Onimawo, IA., Oteno, F., Orokpo, G. and Akubor, PI. (2003). Physiochemical and nutrient evaluation of African bush mango (Irvingia gabonensis) seeds and pulp. Plant Foods for Human Nutrition .58:1-6. https://doi.org/10.1023/B:QUAL.0000040320.33549.df.

      [6] Adjanohoun, JE., Aboubakar, N., Dramane, K., Ebot, ME., Ekpere, JA., Enow-Orock, EG., Focho, D., Gbile, ZE., Kamanyi, A., Kamsu Kom, J., Keita, A., Mbenkum, T., Mbi, CN., Mbiele, AL., Mbome, IL., Miburu, NK. and Nancy, WL. (1996). Traditional medicine and pharmacopoeia: contribution to ethnopharmacological and floristic studies in Cameroon. OAU/STRC. Lagos (Nigeria).

      [7] Okolo, CO., Johnson, PB. Abdurahman, EM. Abdu-Aguye, I. and Hussaini, I.M. (1995). Analgesic effect of Irvingia gabonensis stem bark extract. Journal of Ethnopharmacology. 45(2):125-129. https://doi.org/10.1016/0378-8741(94)01199-A.

      [8] Ngondi, JL., Oben, JE. and Minka, SR. (2005). The effect of Irvingia gabonensis seeds on body weight and blood lipids of obese subjects in Cameroon. Lipids in Health and Disease. 4:12. https://doi.org/10.1186/1476-511X-4-12.

      [9] Kuete, V., Wabo, GF. Ngameni, B., Mbaveng, AT., Metuno, R., Etoa, FX. Ngadjui, BT., Beng, VP. Meyer, JJ. and Lall, N. (2007). Antimicrobial activity of the methanolic extract, fractions and compounds from the stem bark of Irvingia gabonensis (Ixonanthaceae). Journal of Ethnopharmacology. 114(1):54-60. https://doi.org/10.1016/j.jep.2007.07.025.

      [10] Agbor, GA., Oben, JE. Ngogang, JY. Xinxing, C. and Vinson, JA. (2005). Antioxidant capacity of some herbs/spices from Cameroon: a comparative study of two methods. Journal of Agricultural and Food Chemistry. 53(17):6819-6824. https://doi.org/10.1021/jf050445c.

      [11] Ojo, OA. Ajiboye, BO. Oyinloye, BE. and Ojo, AB. (2014a). Prophylactic Effects of Ethanolic Extract of Irvingia gabonensis Stem Bark against Cadmium-Induced Toxicity in Albino Rats. Advances in Pharmaceutics, 2014: 1-8. https://doi.org/10.1155/2014/894610.

      [12] Ojo, OA. Ajiboye, BO. Oyinloye, BE. and Ojo, AB. (2014b). Hematological Properties of Irvingia gabonensis in Male Adult Rats. Journal of Pharmaceutical and Scientific Innovation, 3(5): 434-436. https://doi.org/10.7897/2277-4572.035190.

      [13] Ewere, EG, Uka, E and Usunobun, U. (2016a). Phytochemical Composition, In vitro Antioxidant Activity and Acute Toxicity of Irvingia gabonensis O’Rorke Baill Ethanolic Leaf Extract. International Journal of Biological Research. 4 (1): 36-41.

      [14] Ewere, EG. Oyebadejo, SA. and Peter, VC. (2016b). Ethanolic Leaf Extract of Irvingia gabonensis (O’ Rorke) Baill Protects against Nephrotoxicity and Hepatotoxocity in Cadmium-induced Wistar Albino Rats. International Journal of Pharmacology and Toxicology. 4(2): 105-110. https://doi.org/10.14419/ijpt.v4i2.6186.

      [15] AOAC (Association of Official Analytical Chemists). (2004). Official method of analysis. 15th Edition, Washington D.C., U.S.A.

      [16] Harbone, J.B. (1998). Phytochemical Methods: a guide to modern techniques of plant analysis. Chapman and Hall, London. Pp 60-66.

      [17] Sofowora, A. (2006). Medicinal plant and traditional medicine in Africa Spectrum Book Limited Ibadan Nigeria.

      [18] Anonymous (1990). Official methods of analysis. 15th Edition. Arlington: Association of Official Analytical Chemists.

      [19] Sparkman, DH. Stein, WH. and Moore, S. (1958). Automatic recording apparatus for use in Chromatography of amino acids, Analitical Chemistry. 30(7): 1191-1206. https://doi.org/10.1021/ac60139a006.

      [20] World Health Organisation (2007). Protein and Amino Acid Requirements in Human Nutrition. Report of a Joint WHO/FAO/UNU Expert Consultation on Protein and Amino Acid Requirements in Human Nutrition, 2002: Geneva, Switzerland. Pp 135-178.

      [21] Pandey, M, Abidi, AB, Singh, S. and Singh, RP (2006). Nutritional Evaluation of Leafy Vegetable Paratha. Journal of Human Ecology. 19(2): 155-156.

      [22] Aja, PM., Okaka, ANC., Ibiam, UA. Uraku, AJ. and Onu, PN. (2010). Proximate Analysis of Talinum triangulare (Water Leaf) Leaves and its Softening Principle. Pakistan Journal of Nutrition, 9 (6): 524-526. ISSN 1680-5194 https://doi.org/10.3923/pjn.2010.524.526.

      [23] Isitua, CC., Lozano, MJS. Jaramillo, CJ. and Fausto Dutan, F. (2015). Phytochemical and nutritional properties of dried leaf powder of Moringa oleifera Lam. from machala el oro province of Ecuador. Asian Journal of Plant Science and Research. 5(2):8-16. ISSN : 2249-7412

      [24] Nweze, NO and Nwafor, F. (2014). Phytochemical, Proximate and Mineral Composition of Leaf Extracts of Moringa oleifera Lam. from Nsukka, South-Eastern Nigeria. IOSR Journal of Pharmacy and Biological Sciences. 9(1): 99-103. E-ISSN: 2278-3008, p-ISSN: 2319-7676.

      [25] Usunobun, U., Okolie, NP, Anyanwu, OG. and Adegbegi, AJ. (2014). Phytochemical Screening and Proximate Composition of Annona muricata Leaves. European Journal of Botany Plant Science and Pathology. 2(1): 18-28. ISSN 2055-8147

      [26] Valdez-Solana, MA. Mejía-García, VY. Téllez-Valencia, A., García-Arenas, G., Salas-Pacheco, J., Alba-Romero, JJ. and Sierra-Campos, E. (2015). Nutritional Content and Elemental and Phytochemical Analyses of Moringa oleifera Grown in Mexico. Journal of Chemistry 2015: 1-9. https://doi.org/10.1155/2015/860381.

      [27] Gafar, MK., Itodo, AU. Atiku, FA. Hassan AM. and Peni, IJ. (2011). Proximate Acidic Mineral Composition of the Leaves of Hairy Indigo. In: 34th Annual International Conference Proceedings of Chemical Society of Nigeria. September 19–23, 2011.

      [28] Aletor, VA. and Fasuyi, AO. (1999). Nutrient Composition and processing Effects on Cassava Leaf (Manihot esculenta cranzt) Anti-nutrients. In: Proceedings of the 2nd Annual Conference of Animal Science Association of Nigeria (ASAN), Airport Hotel, Lagos. September 15 – 17; Pp 231-242.

      [29] Onwuka, GI. (2005). Food Analysis and Instrumentation; Theory and Practice, Naphthalic Prints, Surulere, Lagos, Nigeria, pp 219- 230.

      [30] Asibey-Berko, E. and Tayie, F. (1999). Proximate analysis of some underutilized Ghanaian Vegetables. Ghana Journal of Science. 39: 91-92. Doi: 10.4314/gjs.v.39i1.15862.

      [31] Ruberto, G. and Baratta, MT. (2000). Antioxidant activity of selected essential oil components in two lipid model systems. Food Chemistry, 69:167-174. https://doi.org/10.1016/S0308-8146(99)00247-2.

      [32] Malhotra, VK. (1998). Biochemistry for Students. Tenth Edition. Jaypee Brothers Medical Publishers (P) Ltd, New Delhi, India.

      [33] Murray, RK. Granner, DK., Mayes, PA. and Rodwell, VW. (2000). Harper’s Biochemistry, 25th Edition, McGraw-Hill, Health Profession Division, USA.

      [34] Ozcan. M. (2003). Mineral Contents of some Plants used as condiments in Turkey. Food Chemistry, 84: 437-440. https://doi.org/10.1016/S0308-8146(03)00263-2.

      [35] Soetan, KO. Olaiya, C.O. and Oyewole, O.E. (2010). The importance of mineral elements for humans, domestic animals and plants: A review. African Journal of Food Science, 4(5): 200-222. ISSN 1996-0794

      [36] Food and Agriculture Organisationof the United Nations (2001). Human Vitamin and Mineral Requirements. Report of a joint FAO/WHO expert consultation Bangkok, Thailand. Pp 151-267.

      [37] Usunobun, U. and Ewere, EG. (2016). Phytochemical Analysis, Mineral Composition and In Vitro Antioxidant Activities of Chromolaena Odorata Leaves. ARC Journal of Pharmaceutical Sciences, 2(2): 6-10.

      [38] Usunobun U. and Okolie NP. (2015). Phytochemical, trace and mineral composition of Vernonia amygdalina leaves. International Journal of Biological and Pharmaceutical Research. 6(5), 393-399.

      [39] Beard, JL. (2001). Iron biology in immune function, muscle metabolism and neuronal functioning, Journal of Nutrition. 131: 5685-5695.

      [40] Sodamade, A., Bolaji, OS. and Adeboye, OO. (2013). Proximate Analysis, Mineral Contents and Functional Properties of Moringa oleifera Leaf Protein Concentrate. IOSR Journal of Applied Chemistry (IOSR-JAC), 4(6):47-51. E-ISSN: 2278-5736.

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    Ewere, E., Etim, O., & Usunobun, U. (2016). Proximate composition, mineral content and amino acid profile of Irvingia gabonensis O’Rorke baill leaf. International Journal of Scientific World, 5(1), 23-27. https://doi.org/10.14419/ijsw.v5i1.6969