Development and validation of UV spectrophotometric method for simultaneous estimation of nitrate and nitrite in water, soil, drug and vegetable samples
A sensitive and specific spectrophotometric method has been developed for the simultaneous determination of nitrate and nitrite at trace levels. The proposed method is based on diazotization of nitrite with a pharmaceutical compound-metoclopramide (4-amino-5-chloro-N-(2-(diethylamino)ethyl)- 2-methoxybenzamide) in acid medium to form diazonium ion which is coupled with ethyl acetoacetate (EAA) in basic medium to form azo dye, showing absorption maxima at 437.5 nm. Different variables affecting the reactions are optimized. The method is precise and accurate and has been applied to water, soil, drug and vegetable samples. The results of the investigated method using metoclopramide were compared with available official literature method. The results obtained by the proposed method agree well with the standard established method.
Keywords: Diazotization, Nitrate and Nitrite, Spectrophotometric Estimation.
Liang B, Iwatsuk M, & Fukasawa T (1994) Catalytic spectropho-tometric determination of nitrite using the chlorpromazine hydrogen-peroxide redox reaction in acetic-acid medium. Analyst 119 (9), 2113–2117.
Gray WV, & Stephen JF, (2001) Environmental Chemistry, A Global Perspective, 1st edn (Oxford University Press, London, UK).
Cassens RG, (1995) Use of sodium nitrite in cured meats today. Food Technol 49, 72-80.
Cassens RG (1997) Residual nitrite in cured meat. Food Technol. 51:53-55.
Cassens RG (1999) Composition and safety of cured meats in the USA. Food Chem 59, 561-566.
Ignarro LJ, Fukuto JM, Griscavage JM, Rogers NE, Byrns RE (1993) Oxidation of nitric oxide in aqueous solution to nitrite but not nitrate: comparison with enzymatically formed nitric oxide from L-arginine. Proc Natl Acad Sci USA 90, 8103–8107.
Nathan C (1992) Nitric oxide as a secretary product of mammalian cells. FASEB J 6, 3051–3064.
Patty EA (1963) Industrial Hygiene and Toxicology, Interscience, New York, 2, p. 917.
U.S. Environmental Protection Agency Pesticides and Toxic Substances (1991) R.E.D. Facts: Inorganic nitrates/Nitrite (Sodium and Potassium Nitrates). http://www.epa.gov/oppsrrd1/REDs/factsheets/4052fact.pdf.
World Health Organization (2006) International Program on Chemical Safety, Environmental Health Criteria 5: Nitrates, Nitrites, and N-Nitroso Compounds. http://www.inchem.org/documents/pims/chemical/pimg016.htm.
U.S. Environmental Protection Agency Ground Water and Drinking Water (2006) Consumer Factsheet on: Nitrates/Nitrites. Available at http://www.epa.gov/safewater/dwh/c-ioc/nitrates.html.
Virtanen SM, et al. (1994) Nitrate and nitrite intake and the risk for type 1 diabetes in Finnish children. Childhood Diabetes in Finland Study Group. Diabet.Med 11(7), 656-662.
Gupta SK, et al. (2001) recurrent diarrhea in children living in areas with high levels of nitrate in drinking water. Arch.Environ.Health 56 (4), 369-373.
Gupta SK, et al. (2000) recurrent acute respiratory tract infections in areas with high nitrate concentrations in drinking water. Environ.Health Perspect. 108(4), 363-366.
Ali A. Ensafi and Kazemzadeh (1999) Simultaneous determination of nitrite and nitrate in various samples using flow injection with spectrophotometric detection. Analytical Chimica Acta 382, 15-21.
Murad I.H. Helaleh, Takashi Korenaga (2000) IC method for simultaneous determination of nitrate & nitrite in human saliva. Journal of chromatography B, 744, 433-437.
Hitoshi Kodamatani, Shigeo Yamazaki (2009) Selective determination method for measurement of nitrite & nitrate in water samples using high-performance liquid chromatography with post-column photochemical reaction & chemiluminescence detection. Journal of Chromatography A 1216, 3163-3167.
Vincenzo Di Matteo, Ennio Esposito (1997) Methods for the determination of nitrite by HPLC with ED. Journal of chromatography A, 789, 213-219.
Keiichi Fukushi, Koji Tada , Sahori Takeda, Shin-ichi Wakida, Masataka Yamane, Kunishige Higashi (1999) Simultaneous determination of nitrate and nitrite ions in seawater by capillary zone electrophoresis using artificial seawater as the carrier solution. Journal of Chromatography A, 838 303–311
Jain AK, Jahan M. and Tyagi V (1990) Construction and assessment of some percolate-selective liquid membrane electrodes. Anal. Chim. Acta. 231, 69-75.
Aniruddha Pisal, Method 354.1, Water and Environmental Analysis, LAMBDA XLS and XLS+ UV –Visible Spectrophotometers, Perkin Elmer manual.
APHA, (1995) Standard Methods for the Examination of Water and Waste Water, 19th ed, American Public Health Association, Washington DC.
AOAC, Method 36.1.21 (1995) 16th ed, Official Methods of Analysis, Washington, DC.
Christian GD (2001) Analytical Chemistry, fifth edn, John Wiley & Sons (Asia) Pvt. Ltd. 154.
Suchandra Biswas., Bhaskar Chowdhury. And Bidhan Chandra Ray (2004) A novel spectrofluorimetric method for the ultra-trace analysis of nitrite and nitrate in aqueous medium and its application to air, water, soil and forensic samples, Talenta 64, 308-312.
Nidal A, Zatar, Maher A, Abu-Eid. And Abdullah FEid (1999) Spectrophotometric determination of nitrite and nitrate using phosphomolybdenum blue complex. Talenta, 50, 819-826.
Wang H, Yang W, Liang SC, Zhang ZM, and Zhang HS, (2000) Spectrofluorimetric determination of nitrite with 5,6-diamino-1,3-naphthalene disulfonic acid. Anal. Chim. Acta. 419, 169.
Bremner JM (1965) Methods of soil analysis, part 2. C. A. Black etal. (Eds.) ASA 9, 1179.
Padmarajaiah Nagaraja, Mattighatta Shivaswamy and Hemantha Kumar (2002) Spectrophotometric determination of nitrate in polluted water using a new coupling reagent. Analytical Sciences, 18.
Chand Pasha and Narayana B (2010) A Simple Spectrophotometric Method for the Determination of Mosapride by Diazotization Method Yanbu Journal of Engineering and Science 1, 68-73.
Sharma YR (2009) Elementary organic spectroscopy, S. Chand & Company, ISBN: 81-219-2884-2.
Buddha Ratna Shrestha and Raja Ram Pradhananga (2009) Spectrophotometric method for the determination of paracetamol. J. Nepal Chem. Soc. 24
Rodica Diaconu, Marieta Vasilov, Gabriela Mancas (2001) Colorimetric Assay of Salivary Nitrite Content. Jurnal De Medicina Preventiva 9 (1), 74-78.
Beckett AH, and Stenlake J.B., Eds. (2004) In Practical Pharmaceutical Chemistry, 4th Edn, Part two, CBS Publication: 275, 276.
Veena K. & Narayana (2009) Spectroscopic determination of nitrite using new coupling agents. Indian Journal of Chemical Technology 16, 89-92.