Investigation of immobilization and hydrolytic properties of pectinase onto chitosan-PVA copolymer

  • Authors

    • Inderjeet Kaur H.P. University, Shimla 171005
    • Reena Gupta H.P. University, Shimla 171005
    • Anupam Lakhanpal H.P. University, Shimla 171005
    • Atul Kumar H.P. University, Shimla 171005
    2014-08-01
    https://doi.org/10.14419/ijac.v2i2.3046
  • Copolymer based on natural polysaccharide, Chitosan, Chs and synthetic polymer, poly vinyl alcohol, PVA was synthesized using ammonium persulfate as radical initiator and sodium bicarbonate as foaming agent. Reaction conditions were optimized based on swelling percentage of copolymer. The copolymer, (Chs-co-PVA), was characterized by FTIR, SEM and XRD methods and was used as a support for immobilizing pectinase. The immobilized enzyme was found to be more stable than free enzyme and has a good binding efficiency (88.29%) with the copolymer. The effect of temperature, pH and metal ions on the activity of the bound enzyme towards hydrolysis of poly-?-(1-4)-D-galacturonic acid (PGA) was investigated.

    Keywords: Chitosan-PVA Copolymer, Pectinase, Immobilization, Hydrolysis, Polygalacturonic Acid.

  • References

    1. Bajpai A and Manish S (2006) Preparation and Characterization of Novel pH-Sensitive Binary Grafted Polymeric Blends of Gelatin and Poly (vinyl alcohol): Water Sorption and Blood Compatibility Study. Journal of Applied Polymer Science 100, 599-617. http://dx.doi.org/10.1002/app.23370.
    2. Cascone MG, Barbani N, Cristallini C, Giusti P, Ciardelli G and Lazzeri L (2001) Bioartificial polymeric materials based on polysaccharides. Journal of Biomaterial Science Polymer Edition 12(3), 267.
    3. Coluccio ML, Ciardelli G, Berton F, Silvestri D, Cristallini C, Giusti P and Barbani N (2006) Enzymatic Erosion of Bioartifi-cial Membranes to Control Drug Delivery. Macromolecular Bi-oScience 6(6), 403-411. http://dx.doi.org/10.1002/mabi.200600022.
    4. Francois NJ, Allo S, Jacobo SE and Daraio ME (2007) Compo-sites of polymeric gels and magnetic nanoparticles: preparation and drug release behavior. Journal of Applied Polymer Science 105, 647–55. http://dx.doi.org/10.1002/app.26321.
    5. Kumar K, Tripathi BP and Shahi VK (2009) Crosslinked chi-tosan/polyvinyl alcohol blend beads for removal and recovery of Cd (II) from wastewater. Journal of Hazardous Materials 172, 1041–1048. http://dx.doi.org/10.1016/j.jhazmat.2009.07.108.
    6. Lei CX, Hu SQ, Shen GL and Yu RQ (2003) Immobilization of horseradish peroxidase to a nano-Au monolayer modified chi-tosan-entrapped carbon paste electrode for the detection of hydrogen peroxide. Talanta 59, 981–993. http://dx.doi.org/10.1016/S0039-9140 (02)00641-0.
    7. Lei Z and Bi S (2007) Preparation and properties of immobilized pectinase onto the amphiphilic PS-b-PAA diblock copolymers. Journal of Biotechnology 128, 112–119. http://dx.doi.org/10.1016/j.jbiotec.2006.09.002.
    8. Lei Z and Jiang Q (2011) Synthesis and properties of immobi-lized pectinase onto the macroporous polyacrylamide micro-spheres. Journal of Agricultural Food Chemistry 59(6), 2592–2599. http://dx.doi.org/10.1021/jf103719t.
    9. Liang S, Liu L, Huang Q and Yam KL (2009) Preparation of single or double-network chitosan/poly (vinyl alcohol) gel films through selectively cross-linking method. Carbohydrate Polymer 77, 718–724. http://dx.doi.org/10.1016/j.carbpol.2009.02.007.
    10. Lowry OH, Rosenbrough NJ, Farr AL and Randall R (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265.
    11. Majeti NV and Kumar R (2000) A review of chitin and chitosan. Reactive and Functional Polymer 46, 1. http://dx.doi.org/10.1016/S1381-5148 (00)00038-9.
    12. Nelson NA (1944) Photometric adaptation of the somogyi method for determination of glucose. Journal of Biological Chemistry 153, 375.
    13. Ngah W, Kamari WS and Koay AYJ (2004) Equilibrium and kinetics studies of adsorption of copper (II) on chitosan and chitosan/PVA beads. International Journal of Biological Mac-romolecules 34, 155–161. http://dx.doi.org/10.1016/j.ijbiomac.2004.03.001.
    14. Oh JK, Drumright R, Siegwart DJ and Matyjaszewski K (2008) the development of microgels/nanogels for drug delivery applications. Progress in Polymer Science 33, 448–477. http://dx.doi.org/10.1016/j.progpolymsci.2008.01.002.
    15. Prabaharan M and Mano JF (2005) Chitosan-based particles as controlled drug delivery systems. Drug Delivery 12, 41–57. http://dx.doi.org/10.1080/10717540590889781.
    16. Rehman HU, Aman A, Zohra RR and Qader SAU (2014) Immobilization of pectin degrading enzyme from Bacillus licheniformis KIBGE IB-21 using agar-agar as a support. Carbohydrate Polymers 102, 622– 626. http://dx.doi.org/10.1016/j.carbpol.2013.11.073.
    17. Roy I, Sardar M and Gupta, MN (2003) Evaluation of a smart bioconjugate of pectinase for chitin hydrolysis. Biochemical Engineering Journal 16 (3), 329–335. http://dx.doi.org/10.1016/S1369-703X (03)00112-8.
    18. Somogyi M (1952) Notes on sugar determination. Journal of Biological Chemistry 195, 19.
    19. Szaniawski AR and Spencer HG (1997) Affects of immobilized pectinase on the microfiltratin of dilute pectin solutions by macroporous titania membranes: resistance model interpretation. Journal of Membrane Science 127, 69–76. http://dx.doi.org/10.1016/S0376-7388 (96)00299-2.
    20. Wang Q, Du YM and Fan LH (2005) Properties of chi-tosan/poly (vinyl alcohol) films for drug-controlled release. Journal of Applied Polymer Science 96, 808–813. http://dx.doi.org/10.1002/app.21518.
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  • How to Cite

    Kaur, I., Gupta, R., Lakhanpal, A., & Kumar, A. (2014). Investigation of immobilization and hydrolytic properties of pectinase onto chitosan-PVA copolymer. International Journal of Advanced Chemistry, 2(2), 117-123. https://doi.org/10.14419/ijac.v2i2.3046