Development and validation of HPTLC method for the estimation of cefadroxil in bulk and tablet dosage form

 
 
 
  • Abstract
  • Keywords
  • References
  • PDF
  • Abstract


    A novel and simple HPTLC method has been developed for the estimation of Cefadroxil (CFD) in bulk and pharmaceutical dosage form on silica gel precoated aluminum 60F254 plates, (10 cm x10 cm) with 0.25 mm thickness. The separation was carried out using ethyl acetate: chloroform (8:2v/v) as mobile phase. The Chromatographic scanning was carried out at 254 nm. The Rf (± SD) values were found to be 0.38 ± 0.05 for CFD. The linearity for analyte was in the range 100-500 ng/band for CFD with correlation coefficients, r2 = 0.9996. The percentage recovery obtained for CFD was in the range of 99.28-100.13% respectively. The proposed method was optimized and validated as per the ICH guidelines17.

     

     


  • Keywords


    Cefadroxil; HPTLC; Method Validation; Tablet Dosage Form.

  • References


      [1] https://en.wikipedia.org/wiki/Cefadroxil

      [2] https://www.drugbank.ca>drugs.

      [3] Patel C, Patel K, Sen DJ, Badmanaban R, Parikh A. Development and validation of spectroscopic methods for the estimation of Cefadroxil in tablet dosage forms. J Chem Pharm Res. 2010; 2(2):163-67.

      [4] Sethuraman S, Radhakrishnan K, Venkateswarlu V, Sravani M, Ramathulasi G, Bhanuteja S. Estimation and degradation monitoring of Cefadroxil in pharmaceutical dosage form by using UV-spectroscopy. Asian J Res Bio Pharm Sci. 2014; 2(1):27-33.

      [5] Pradip D, Santosh J, Sumit G, Laxmi J. Development and validation of UV spectroscopic estimation of Cefadroxil in bulk and tablet dosage form using area under curve method. Indo American J Pharm sci. 2015; 2(2):581-6.

      [6] Dey S, Kalyani K, Samyuktha B, sahoo SK, Mohapatra S, Murthy PN et al. Development and validation of a UV-vis spectrophotometric method for the estimation and degradation monitoring of Cefadroxil in bulk and pharmaceutical dosage forms. Int J Chem Res. 2010; 1(1):29-34.

      [7] Shantier SW, Gadkariem EA, Ibrahim KE, EL-Obeid HA. Spectroscopic determination of Cefadroxil in bulk and dosage form using sodium hydroxide. J Chem. 2011; 8(3):1314-22. https://doi.org/10.1155/2011/568620.

      [8] Kumar CA, Gurupadayya BM, Sloka SN, Chandan RS, Thejaswini JC. Colorimetric determination of Cefadroxil and ceftriazone in pharmaceutical dosage forms. Tropical J Pharm Res, 2011; 10(1):81-88. https://doi.org/10.4314/tjpr.v10i1.66546.

      [9] Rao KG, Uma B, Shankar B, Naik BM. Development and validation of RP-HPLC method for the estimation of Cefadroxil Monohydrate in bulk and its tablet dosage form. J Adv Pharm Edu & Res. 2014; 4(1):71-74.

      [10] Anjum A, Shetty SK, Ahmed M, Sridhar BK, Vijaya KM. Development and validation of RP-HPLC method for the quantitative estimation of Cefadroxil monohydrate in bulk and pharmaceutical dosage forms. Int J Chem Sci. 2012; 10(1):150-58.

      [11] Rahim N, Naqui SB, Shakeel S, Iffat W, Muhammad IN. determination of Cefadroxil in tablet/capsule formulations by a validated reverse phase high performance liquid chromatographic method. Pakistan J Pharm Sci. 2015; 28(4):1345-49.

      [12] Shukla RS, Pandey S, Bangale R. Novel HPLC analysis of Cefadroxil in bulk formulation. Asian J Pharm. 2008; 2(2):106-109. https://doi.org/10.4103/0973-8398.42497.

      [13]Vittaladevaram V, Pragada H. Development of method for analysis and quantification of Cefadroxil in different pharmaceutical formulations using HPLC. Int J Pharm Bio Sci. 2017; 7(1):27-31. https://doi.org/10.21276/ijpbs.2017.7.1.4.

      [14]Margo BA, Salgado HR, Development and validation of an innovative method for the determination of Cefadroxil monohydrate in capsules. World J Pharm Sci. 2017; 6(8):2074-91. https://doi.org/10.20959/wjpps20178-9875.

      [15]Pisal VB, Deshpande PB, Gandhi SV, Bhangale Y. High performance thin layer chromatographic determination of Potassium clavulanate and Cefadroxil in combined dosage form. Der Pharmacia Sinica. 2011; 2(2):79-85.

      [16] Dhoka MV, Chopade SS. Method development & comparative statistical evaluation of HPLC & HPTLC method for simultaneous estimation of Cefadroxil monohydrate & Ambroxol hydrochloride. Indo Global J Pharm Sci. 2012; 2(2):203-12.

      [17] ICH, Q2 (R1) Validation of Analytical Procedures: text and methodology;2005.


 

View

Download

Article ID: 30963
 
DOI: 10.14419/ijac.v8i2.30963




Copyright © 2012-2015 Science Publishing Corporation Inc. All rights reserved.