Comparative phytochemical screening and antioxidant activity of lemon grass and sweet wormwood leaves extract
Keywords:Antioxidant, Free Radical, Phytochemical, Lemon Grass, Sweet Wormwood.
Sweet wormwood (Artemisia annua) and lemongrass (Cymbopogon citratus) leaves were extracted with methanol, evaporated to dryness and processed for antioxidant activity and phytochemical screening. Phosphomolybdenum Reagent was prepared using standard procedure. An aliquot mixture of 2mL of extract solution (1000Âµg/mL, 500Âµg/mL, 250Âµg/mL, 125Âµg/mL, 62.5Âµg/mL, 31.25Âµg/mL and 15.625Âµg/mL) was mixed with 1.8mL of reagent solution. The samples tubes were incubated at 95Â°C for 90 minutes. The absorbance of the aqueous solutions was measured at 695nm against blank. Antioxidant activities of the plants extract were also measured in terms of hydrogen donating radical scavenging ability using the stable DPPH method. Absorbance was recorded at 517 nm against blank using a UV-Vis spectrophotometer. The result of in vitro antioxidant potential of the methanolic leaves extract showed more increase in concentration the higher the absorbance. Qualitative Phytochemical Analysis of Lemon Grass and Sweet wormwood shows the presence of phytochemical in lemongrass and sweet wormwood such as flavonoid, tannin, steroid, saponins and cardiac glycoside.
 Yu-Jie Z, Gui-Fang D, Xiang-Rong X, Shan W, Sha L and Hua-Bin L (2013) Chemical components and bioactivities of cape gooseberry (physalis peruwiana). International Journal of Food Nutrition and Safety 3(1): 15-24.
 LÃ¡szlÃ³ S, Attila H, Ã‰va S (2005) Presumable protective role of peroxidase and polyphenol oxidase enzymes against freezing stress in peach (Prunus persica L. Batsch). - Acta Bioloca Szegediensis 49(1-2): 121-122.
 Lobo V, Patil A, Phatak A and Chandra N (2010) Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews,4,8,118-126. https://doi.org/10.4103/0973-7847.70902.
 Miral D, Pawel J (2012) Mechanisms of free radical-induced damage to DNA. Free Radical Research 46(4):382-419. https://doi.org/10.3109/10715762.2011.653969.
 Satish B N and Dilipkumar P (2015) Free radicals, natural antioxidants, and their reaction mechanisms. Royal Society of Chemistry 5, 27986-28006. https://doi.org/10.1039/C4RA13315C.
 Azwanida N.N (2015) A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Medicinal and Aromatic Plants 4: 2-4. https://doi.org/10.4172/2167-0412.1000196.
 Prieto P, Pineda M and Anguiler M (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E. Analytical Biochemistry 269:337-341. https://doi.org/10.1006/abio.1999.4019.
 Zhu K, Zhou H, and Qian H (2006) Antioxidant and free radical scavenging activities of wheat germ protein hydrolysates (WGPH) prepared with alcalase. Process Biochemistry 41(6):1296â€“302. https://doi.org/10.1016/j.procbio.2005.12.029.
 Vishnu R, Nisha R, Jarmuna S, and Paulsamy S (2013) Quantification of total phenolics and flavonoid and evalution of in vitro antioxidant properties of Methanolic leaf extract of tarenna asitatic an endemic medicinal plant species of Maruthamali hills, western Ghats,Tami Nadu. Journal of Research in plant Science 2(2):196-204.
 Jamuna S, Subramaniam P, Krishnamoorthy K (2014) Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities. Asian Pacific Journal of Tropical Biomedicine 4(Suppl 1): S359-S367. https://doi.org/10.12980/APJTB.4.2014C1030.
 Kaur J, Xavier R, Marimuthu, Leng K, Rajasekaran A, Kathiresan S, and Sreeramanan S (2010) Preliminary investigation on the antibacterial activity of mango (Mangifera indica L: Anacardiaceae) seed kernel. Asian Pacific Journal of Tropical Medicine3(9): 707-710. https://doi.org/10.1016/S1995-7645(10)60170-8.
 Thiago A, Nelson L, Elba L, andUlysses P (2008) New approach to study medicinal plants with tannins and flavanoids contents from the local knowledge. Journal of Ethnopharmacology; 120: 72-80. https://doi.org/10.1016/j.jep.2008.07.032.
 Sulekha M, Satish Y, Sunita Y, and Rajesh K. N (2009) Antioxidants: A Review. Journal of Chemical and Pharmaceutical Research 1 (1):102-104.