Isolation of natural caffeine from liptonâ„¢ black tea through acid-base liquid-liquid extraction approach, its medical significance and its characterization by thin layer chromatography and IR analysis

  • Authors

    • M. Fazal-ur-Rehman Department of Chemistry, Lahore Garrison University, Lahore, Pakistan
    • Iqra Qayyum Department of Chemistry, Lahore Garrison University, Lahore, Pakistan
    • Dr. Manzar Zahra Department of Chemistry, Lahore Garrison University, Lahore, Pakistan
    2021-03-28
    https://doi.org/10.14419/ijac.v9i1.31458
  • Liquid-Liquid Extraction, Sublimation, Alkaloids, Structure Analogous, Gallic Acid.
  • To isolate the caffeine from Liptonâ„¢ Black Tea Brand, a sequence of practices was applied. An Acid-Base Liquid-Liquid Extraction approach carried out to force the caffeine to be isolated in upper organic layer as extractant. By this method, first caffeine was isolated from tea bags through passing different steps of extraction, then caffeine was isolated from tea with the use of both Solid-Liquid approach as well as liquid-liquid extraction approach. A product of 0.05 g of pure caffeine was obtained giving the percentage yield or percent recovery of 1.22%. Calculated percent recovery was 1.2 %, this percentage yield clarified that in this tea brand, very small caffeine is investigated, this deduces a significant loss of product throughout the procedure which are due to formation of emulsions and not due to washing thoroughly with DCM to extract maximum yield. It is also significant to be considered that reactions of precursor with solvent pair may not be completed, so 100% yield is not conceivable. Due to much transfers in all processes, this loss might be occurred. Due to much transfers in all processes, this loss might be occurred that’s why repeated the process three times again. It is also revealed that as much water was added which decreased the concentration of Caffeine. On analysis with IR, Peak at f=3000Hz indicates the presence of -NH2 and -CONH2 groups while the peaks at f=1600 Hz and f=1750 Hz indicates the presence of alkene portion of the caffeine molecule which concluded that Caffeine is a purine base.

     

     

  • References

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      [1] Acree Jr, W. E. (2018). Comments on “Thermodynamic modeling studies of aqueous solubility of caffeine, gallic acid and their cocrystal in the temperature range of 303 K–363 Kâ€. Fluid Phase Equilibria, 463, 32-33. https://doi.org/10.1016/j.fluid.2018.01.037.

      [2] Azevedo, R. S. A., Teixeira, B. S., da Silva Sauthier, M. C., Santana, M. V. A., dos Santos, W. N. L., & de Andrade Santana, D. (2018). Multivariate analysis of the composition of bioactive in tea of the species Camellia sinensis. Food Chemistry. https://doi.org/10.1016/j.foodchem.2018.04.030.

      [3] Barone, J., & Roberts, H. (1984). Human consumption of caffeine. In Caffeine (pp. 59-73). Springer. https://doi.org/10.1007/978-3-642-69823-1_4.

      [4] Brooks, F. (2017). Foundations of Mental Health and Substance Abuse. In Forensic Mental Health (pp. 75-106). Routledge. https://doi.org/10.4324/9781315677460-4.

      [5] Budney, A. J., & Emond, J. A. (2014). Caffeine addiction? Caffeine for youth? Time to act! Addiction, 109(11), 1771-1772. https://doi.org/10.1111/add.12594.

      [6] Bunker, M. L., & McWilliams, M. (1979). Caffeine content of common beverages. Journal of the American Dietetic Association, 74(1), 28-32.

      [7] Castrén, S. (2013). Disordered gambling in Finland: Epidemiology and a current treatment option. THL.

      [8] Clementz, G. L., & Dailey, J. W. (1988). Psychotropic effects of caffeine. American family physician, 37(5), 167-172.

      [9] Cortés, M., Malave, L., Castello, J., Flajolet, M., Cenci, M. A., Friedman, E., & Rebholz, H. (2017). CK2 Oppositely Modulates l-DOPA-Induced Dyskinesia via Striatal Projection Neurons Expressing D1 or D2 Receptors. Journal of Neuroscience, 37(49), 11930-11946. https://doi.org/10.1523/JNEUROSCI.0443-17.2017.

      [10] Costill, D., Dalsky, G. P., & Fink, W. (1978). Effects of caffeine ingestion on metabolism and exercise performance. Medicine and science in sports, 10(3), 155-158.

      [11] Dulloo, A. G., Duret, C., Rohrer, D., Girardier, L., Mensi, N., Fathi, M., Chantre, P., & Vandermander, J. (1999). Efficacy of a green tea extract rich in catechin polyphenols and caffeine in increasing 24-h energy expenditure and fat oxidation in humans–. The American journal of clinical nutrition, 70(6), 1040-1045. https://doi.org/10.1093/ajcn/70.6.1040.

      [12] Faber, N. S., Häusser, J. A., & Kerr, N. L. (2017). Sleep deprivation impairs and caffeine enhances my performance, but not always our performance: How acting in a group can change the effects of impairments and enhancements. Personality and Social Psychology Review, 21(1), 3-28. https://doi.org/10.1177/1088868315609487.

      [13] Goddard, A. (2016). Psychological acculturation, contextual variables of substance related problems, and psychiatric symptoms among Latinos Adler School of Professional Psychology].

      [14] Gorsane, M. A., Reynaud, M., Vénisse, J.-L., Legauffre, C., Valleur, M., Magalon, D., Fatséas, M., Chéreau-Boudet, I., Guilleux, A., & Group, J. (2016). Gambling disorder-related illegal acts: Regression model of associated factors. Journal of behavioral addictions, 6(1), 64-73. https://doi.org/10.1556/2006.6.2017.003.

      [15] Griffiths, R. R., & Woodson, P. P. (1988). Caffeine physical dependence: a review of human and laboratory animal studies. Psychopharmacology, 94(4), 437-451. https://doi.org/10.1007/BF00212836.

      [16] Grosso, G., Godos, J., Galvano, F., & Giovannucci, E. L. (2017). Coffee, caffeine, and health outcomes: an umbrella review. Annual review of nutrition, 37, 131-156. https://doi.org/10.1146/annurev-nutr-071816-064941.

      [17] Khajeh, M., Fard, S., Bohlooli, M., Ghaffariâ€Moghaddam, M., & Khatibi, A. (2017). Extraction of caffeine and gallic acid from coffee by electrokinetic methods coupled with a hollowâ€fiber membrane. Journal of Food Process Engineering, 40(6). https://doi.org/10.1111/jfpe.12565.

      [18] Lin, C., Xia, G., & Liu, S. (2017). Modeling and comparison of extraction kinetics of 8 catechins, gallic acid and caffeine from representative white teas. LWT-Food Science and Technology, 83, 1-9. https://doi.org/10.1016/j.lwt.2017.04.028.

      [19] Malenka, R., Nestler, E., & Hyman, S. (2009). Reinforcement and addictive disorders. Molecular Neuropharmacology: A Foundation for Clinical Neuroscience (2nd ed., pp. 364-375. New York: McGraw-Hill Medical.

      [20] Mehta, V., Verma, P., Sharma, N., Sharma, A., Thakur, A., & Malairaman, U. (2017). Quercetin, ascorbic acid, caffeine and ellagic acid are more efficient than rosiglitazone, metformin and glimepiride in interfering with pathways leading to the development of neurological complications associated with diabetes: A comparative in-vitro study. Bulletin of Faculty of Pharmacy, Cairo University, 55(1), 115-121. https://doi.org/10.1016/j.bfopcu.2016.12.002.

      [21] Mumin, A., Akhter, K. F., Abedin, Z., & Hossain, Z. (2006). Determination and characterization of caffeine in tea, coffee and soft drinks by solid phase extraction and high performance liquid chromatography (SPE–HPLC). Malaysian Journal of Chemistry, 8(1), 045-051.

      [22] Nawrot, P., Jordan, S., Eastwood, J., Rotstein, J., Hugenholtz, A., & Feeley, M. (2003). Effects of caffeine on human health. Food Additives & Contaminants, 20(1), 1-30. https://doi.org/10.1080/0265203021000007840.

      [23] Pohler, H. (2010). Caffeine intoxication and addiction. The journal for nurse practitioners, 6(1), 49-52. https://doi.org/10.1016/j.nurpra.2009.08.019.

      [24] Rainey, J. (1985). Headache related to chronic caffeine addiction. Texas dental journal, 102(7), 29.

      [25] Ruan, Z., Ghorai, D., Zanoni, G., & Ackermann, L. (2017). Nickel-catalyzed C–H activation of purine bases with alkyl halides. Chemical Communications, 53(65), 9113-9116. https://doi.org/10.1039/C7CC05011A.

      [26] Slade, A. (2017). Caffeine and Acetaminophen Concentration and Other Water Quality Parameters Along the Middle to Lower Bear River.

      [27] Tello, J., Viguera, M., & Calvo, L. (2011). Extraction of caffeine from Robusta coffee (Coffea canephora var. Robusta) husks using supercritical carbon dioxide. The Journal of Supercritical Fluids, 59, 53-60. https://doi.org/10.1016/j.supflu.2011.07.018.

      [28] [Record #34 is using a reference type undefined in this output style.]

      [29] Wang, H., Chen, L., Xu, Y., Zeng, Q., Zhang, X., Zhao, Q., & Ding, L. (2011). Dynamic microwave-assisted extraction coupled on-line with clean-up for determination of caffeine in tea. LWT-Food Science and Technology, 44(6), 1490-1495. https://doi.org/10.1016/j.lwt.2011.01.015.

      [30] Williamson, K. L. (2011). Organic Experiments: Macroscale and Microscale. Brooks/Cole.

      [31] Yalwa, I., & Bello, A. (2017). Determination of caffeine content in some varieties of kola nut (C. acuminate). Bayero Journal of Pure and Applied Sciences, 10(1), 247-251. https://doi.org/10.4314/bajopas.v10i1.50S.

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    Fazal-ur-Rehman, M., Qayyum, I., & Manzar Zahra, D. (2021). Isolation of natural caffeine from liptonâ„¢ black tea through acid-base liquid-liquid extraction approach, its medical significance and its characterization by thin layer chromatography and IR analysis. International Journal of Advanced Chemistry, 9(1), 29-33. https://doi.org/10.14419/ijac.v9i1.31458