Detection of interaction between ornidazole and albumin by using thermodynamic parameters

  • Authors

    • Esra MaltaÅŸ Çağıl Selcuk University, Faculty of Pharmacy, Selcuklu, Konya, Turkey
  • Ornidazole, Human Serum Albumin (HSA), Fluorescence Quenching, Stern Volmer Equation, Thermodynamic Parameter.
  • The binding of 1-chloro-3-(2-methyl-5-nitro-1H-imidazole-1-yl) propan-2-ol (Ornidazole) to human serum albumin (HSA) was studied by fluorescence and UV-visible spectroscopy. Interaction of ornidazole (OR) with HSA was identified by Stern-Volmer and Van’t Hoff equations. The binding constant, Kb and the thermodynamic parameters, ∆H, ∆S, and ∆G at different temperatures were calculated by several equations. Data shows that the fluorescence quenching mechanism of HSA with ornidazole may occur via static quenching. The thermodynamic parameters showed that van der Waals interactions and hydrogen bonds are the major forces for the interaction of ornidazole with HSA. The spectral changes of synchronous fluorescence suggested that both the microenvironment of OR and the conformation of HSA concerning their concentrations have changed during binding.




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  • How to Cite

    Maltaş Çağıl, E. (2021). Detection of interaction between ornidazole and albumin by using thermodynamic parameters. International Journal of Advanced Chemistry, 9(2), 161-167.