Bioactive composition and TLC profile data on PAX herbal health tea and PAX herbal diatea

Authors

  • Tunde Ayobami Owolabi University of Benin, Benin City, Edo State.
  • Emmanuel Amodu Federal University Lafia, Nasarawa State

DOI:

https://doi.org/10.14419/ijac.v10i2.32112

Keywords:

Bioactive Composition, Thin Layer Chromatography (TLC) Fingerprint Profile, Herbal Medicine, Quality Control, PAX Herbal Health Tea and PAX Herbal Diatea.

Abstract

Background: The phytoconstituents of herbal drugs are largely influenced by the quality control system used during and post-production processes including the handling of such products. Thin Layer Chromatography is one of such quality control parameters that demonstrate uniqueness and uniformity between various substances, serving as an identity for such substances.  Pax Herbal Health tea (PHT) and Pax Herbal Diatea (PDT) are polyherbal drugs, PHT is used as a tonic for general wellness, while, PDT is used in the management and treatment of diabetes. This study evaluated the different phytoconstituents present and developed Thin-layer chromatography (TLC) fingerprint profiles for PHT and PDT to serve as quality control checks during the production for consistency and market uniqueness after production.

Material and Methods: Qualitative phytochemical and chromatographic analyses were carried out using standard methods.

Results: The phyto-screening revealed the presence of Alkaloid, Flavonoid, Tanin, Terpenoids, Reducing sugar, Steroid, and Cardiac glycoside in PHT while, Saponin, Tanin, Steroids, Reducing sugar, Flavonoid, and Terpenoids were observed in PDT. The TLC finger-print chromatograms of PHT after development with n-Hexane:Ethyl acetate (3:2) showed four distinct components under ultraviolet light at 365 nm, and three spots when sprayed with 20% methanolic sulphuric under visible light, while, PDT developed in n-Hexane:ethyl acetate:methanol (2.5:2:0.5) revealed three fluorescent components at 365 nm and four components after sulphuric acid treatment.

Conclusion: From this present study, identity cards have been designed for PHT and PDT through bioactive composition and TLC profiles which can be used in accessing the quality and consistency of the herbal drugs.

 

 

References

[1] Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol. 2014; 4:177. https://doi.org/10.3389/fphar.2013.00177.

[2] Nigeria Natural Medicine Development Agency, (NNMDA) (2009). Federal Ministry of Science and Technology 9, Kofo Abayomi Street, Victoria Island, Lagos, Nigeria. p. 1-49.

[3] Owolabi TA, Ezenwa KC, Amodu E, Iyoriobhe OC, Okubor PC, Aigbe DP, Okogun JI. Antidepressant Potentials of Aqueous Extract of Voacanga africana stept. ex Eliot (Apocynaceae) Stem Bark. Int. J Curr. Microbiol. App. Sci 2019;8(12): 2623-2629.

https://doi.org/10.20546/ijcmas.2019.812.306

[4] Owolabi TA, Ezenwa KC, Olayioye EY, Iyorhibe OC, Amodu E, Aferuan OF, et al. Adaptogenic (Anti-Stress) Effect of Aqueous Musanga cecropioides (Urticaceae). Int. J Curr. Microbiol. App. Sci 2019;8(10):2558-2565. https://doi.org/10.20546/ijcmas.2019.810.296.

[5] Amodu E, Momoh TB, Otoigiakhi SO, Iyeh VA, Owolabi TA, Ezenwa KC, Olayioye EO, Iyoriobhe OC, Aferuan OF. Ethnobotany and Ethnopharmacology of the Igala kingdom in Kogi East, Nigeria. Taiwania 2020: 65(2): 199‒208.https://doi.org/10.6165/tai.2020.65.199.

[6] Owolabi TA, Ayinde BA. Bioactivity guided isolation and characterization of anti-cancer compounds from the Stem of Musanga cecropioides R. Br. Ex Tedlie (Urticaceae). Journal of Pharmacognosy and Phytochemistry 2021; 10(6): 292-296.

[7] Renato B, Luciana G, Wolf, Gisely C, Lopes, João CP, de Mello. Quality control and TLC profile data on selected plant species commonly found in the Brazilian market Rev. Bras. Farmacogn. Braz. J. Pharmacogn. 2012:22(5). https://doi.org/10.1590/S0102-695X2011005000204.

[8] Mohammad A, Bhawani SA, Sharma S. Analysis of herbal products by thin-layer chromatography: a review. Int J Pharma Bio Sci. 2010:1: 1-50.

[9] Harborne JB (1973). Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. Chapman and Hall, London. p. 279. https://doi.org/10.1007/978-94-009-5921-7_1.

[10] Trease GE, Evans WC (2002). Pharmacognosy. 15th Ed. London: Saunders Publishers; pp. 42–44.

[11] Owolabi TA, Salome OE. Quantification of bioactive constituents of mistletoe leaves (Tapinanthus globiferus a. rich) from four different host plants in the EWU community International journal of advance chemistry. 2021: 10 (1) 1-4.

[12] Sasidharan S, Chen Y, Saravanan D, Sundram KM, Yoga Latha L. Extraction, isolation and characterization of bioactive compounds from plants' extracts. Afr J Tradit Complement Altern Med. 2011; 8(1):1-10. Epub 2010 Oct 2. PMID: 22238476; PMCID: PMC3218439. https://doi.org/10.4314/ajtcam.v8i1.60483.

[13] Panossian A.G., Efferth T., Shikov A.N., Pozharitskaya O.N., Kuchta K., Mukherjee P.K., Banerjee S., Heinrich M., Wu W., Guo D., et al. Evolution of the adaptogenic concept from traditional use to medical systems: Pharmacology of stress and aging related diseases. Med. Res. Rev. 2020; 41:630–703. https://doi.org/10.1002/med.21743.

[14] Azebaze AG, Teinkela JE, Nguemfo EL, Valentin A, Dongmo AB, Vardamides JC. Antiplasmodial activity of some phenolic compounds from Cameroonians Allanblackia. African health sciences. 2015: 15(3), 835–840. https://doi.org/10.4314/ahs.v15i3.18.

[15] Durón RR, Almaguer LC, Garza-Juárez AJ, Cavazos MLS, De-Torres NW. Development and validation of thin-layer chromatographic methods for quality control of herbal products. Acta Chromatogr. 2009: 21: 203-215. https://doi.org/10.1556/AChrom.21.2009.2.2.

Downloads

Published

2022-08-27

Issue

Section

Articles