In vitro callus induction of gerbera from leaf explant

  • Authors

    • Sadia Afrin Jui Bangladesh Jute Research Institute, Dhaka, Bangladesh
    • Md. Mijanur Rahman Rajib Bangabandhu Sheikh Mujibur Rahman Agricultural University
    • M. Mofazzal Hossain Bangabandhu Sheikh Mujibur Rahman Agricultural University
    • Sharmila Rani Mallik Bangladesh Agricultural Research Institute
    • Iffat Jahan Nur Bangladesh Jute Research Institute
    • Jannatul Ferdush Bangladesh Jute Research Institute
    2020-01-11
    https://doi.org/10.14419/ijag.v8i1.30000
  • Callus, Gerbera, Growth Regulators, Leaf Explants, Culture.
  • The experiment was designed to evaluate the effect of growth regulators on leaf explant of Gerbera for callus induction. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), α-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Indole-3-acetic acid (IAA) were used to initiate cultures. These were added to Murashige and Skoog medium in different combinations and concentrations. Leaf explants cultured on MS medium supplemented with BAP+ 2, 4-D+ IAA in T4 treatment & BAP+ 2,4-D in T5 treatment showed the best results for callus induction. On the other hand callus was induced early in the combination of BA+ 2,4-D + IAA hormone in T5, T9 & T8 treatment respectively. The rate of callus induction was very low in BA + NAA combinations but it was much earlier.

     

     

     


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  • How to Cite

    Afrin Jui, S., Mijanur Rahman Rajib, M., Mofazzal Hossain, M., Rani Mallik, S., Jahan Nur, I., & Ferdush, J. (2020). In vitro callus induction of gerbera from leaf explant. International Journal of Advanced Geosciences, 8(1), 1-4. https://doi.org/10.14419/ijag.v8i1.30000