The Effects of MYO-inositol and Indole Butyric Acid (IBA) on the Formation of Pineapples Root (Ananas comosus L.) from Sipahutar North Sumatera In Vitro

  • Authors

    • Arisah Hasanah Postgraduate Program of Biology Department, Faculty of Mathematics and Natural Science, Universitas Negeri Medan
    • Fauziyah Harahap Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan
    • Ramlan Silaban Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan
    2018-07-25
    https://doi.org/10.14419/ijbr.v6i2.13699
  • Root Formation, IBA, MYO-Inositol, Ananas Comosus L., in Vitro Results, The result of the research showed that root time appeared on 3 WAP. The increasing number of leaves and timing of the puppies in 1 WAP. Myo-inositol had significant effects on root c
  • Background: Rooting of pineapple from Sipahutar in vitro is an alternative to obtain superior seeds in large quantities to meet the needs of farmers and consumers.

    Objective: The purpose of this study was to investigate the effect of Myo-inositol and IBA on pineapple rooting (Ananas comosus L.) from Sipahutar in vitro.

    Methods: The research method used a completely randomized factorial design with two factors: Factor I: Myo-inositol consisted of [4] treatment concentrations: M0 = 0 g/l, M0.02 = 0.02 g/l, M0.04 = 0.04 gr/l, M0.06 = 0.06 gr/l. Factor II: IBA growth regulators consisted of [3] treatment concentrations: IB0 = 0 ppm, IB1.5 = 1.5 ppm, IB3 = 3 ppm. The parameters observed were time of root emergence, number of roots, number of leaves, time of leaf emergence, number of tillers, and time of its emergence observed each week, while the height of tillers, shoots, and whole shoots, root length, leaf width and leaf length were observed 12 weeks after planting (WAP).

    Results: The result of the research showed that root time appeared on [3] WAP. The increasing number of leaves and timing of the puppiesin 1 WAP. Myo-inositol had significant effects on root counts in the 0.06 gr/l and root length on Myo-inositol treatment in the 0.04 g/l. IBA did not have a real effect on the entire observation parameters. The interactions of Myo-inositol and IBA had significant effects on root number, number of leaves, leaf length and tiller height.

    Conclusions: Myo-inositol and IBA had significant effects on root number, leaf number, leaf length and shoot height of pineapple’s explant from Sipahutar.

     

  • References

    1. [1] Anonim. 2015 http:/ /hariansib.co /view/ Marsipature Hutanabe/44107/Petani-Sipahutar-Butuh-Bibit-Nenas.html.

      [2] Anonim. 2016). http: //www. Medanbis nisdaily.com/new s/read/2016/06/02/237611/nenas-taput-nyeberang-ke-malaysia/.

      [3] Anonim. 2007). https:// www.merdeka. com/uang/dikaleng-nanas-tapanuli-utara-tembus-ekspor-sizqtx2.html.

      [4] Arlianti T, Syahid S, Kristina N, dan Ros O. 2013. Pengaruh Auksin IAA, IBA, Dan NAA Terhadap Induksi Perakaran Tanaman Stevia (Stevia Rebaudiana) Secara in Vitro. Bul. Littro, 24(2): 57 – 62.

      [5] Arimarsetiowati, R. dan Ardiyani, F. 2012. Pengaruh penambahan auxin terhadap pertunasan dan perakaran kopi arabika perbanyakan Somatik Embriogenesis. Pelita Perkebunan, 28(2): 82 – 90.

      [6] Bella. Suminar, E. Nuraini, A. 2016. Pengujian efektivitas berbagai jenis dan konsentrasi sitokinin terhadap multiplikasi tunas mikro pisang (Musa paradisiaca L.) secara in vitro Jurnal Kultivasi, 15(2): 74 – 80.

      [7] Cerianingsih M. W, Astarini I. A dan Nurjaya I. G. O. 2015. Pengaruh Kombinasi Zat Pengatur Tumbuh Indole-3-Butyric Acid (IBA) Dan 6-Benzil Amino Purin (BAP) Pada Kultur in Vitro Tunas Aksilar Anggur (Vitis vinifera L.) Varietas Prabu Bestari Dan jestro Ag 86. Jurnal Metamorfosa, 2 (1): 1 - 8.

      [8] Duval MF, Noyer JL, Perrier X, Coppens d’Eeckenbrugge G, Hamon P (2001). Molecular diversity in pineapple assessed by RFLP markers. Theor. Appl. Genet.102: 83-90. https://doi.org/10.1007/s001220051621.

      [9] Febriyanti E, Suwirmen dan dris M. 2013 Induksi Perakaran Tunas Tetrastigma rafflesiae Miq. Pada Media Murashige-Skoog Dengan Penambahan Beberapa Konsentrasi Indole-3-Butyric Acid (IBA) secara In Vitro. Jurnal Biologi Universitas Andalas, 2(3): 188 – 193.

      [10] Febriyanti. 2017. Induksi pertumbuhan tuna’s dari eksplan anggrek Dendrobium Heterocarpum Lindl. Dengan pemberian hormon zeatin dan NAA. Jurnal Metamorfosa, 4 (1): 41 – 47.

      [11] Gunawan, L. W. 1992. Teknik Kultur Jaringan Tumbuhan. Departemen Pendidikan dan Kebudayaan. DIKTI – PAU IPB, Bogor.

      [12] Harahap, F. 2011. Kultur Jaringan Tanaman. Medan : Unimed Press.

      [13] Harahap, F dan Nusyirwan. 2012. Induksi Pertumbuhan Nanas (Ananas comosus L.) In Vitro Asal Pangaribuan dengan Pemberian Zat Pengatur Tumbuh Kinetin. Prosiding Semirata BKS PTN-B MIPA : “Peranan MIPA Dalam Pengembangan SDM dan SDA. Hotel Madani-Universitas Negeri Medan.

      [14] Harahap, F., Hasruddin, Suriani, C., Nusyirwan, Siallagan, J. dan Rohyana. 2013. Sterilisasi Eksplan Nanas (Ananas comusus L.) Asal Sipahutar dan Induksi Pertumbuhan dengan Perlakuan Benzil Amino Purin dan Indole Acetic Acid Secara In Vitro. Seminar Nasional ke-22 Perhimpunan Biologi Indonesia. Purwokerto, Jawa Tengah.

      [15] Harahap, F dan Nusyirwan. 2014. Induksi Tunas Nanas (Ananas comusus L. Merr) In Vitro dengan Pemberian Dosis Auksin dan Sitokin yang Berbeda. Jurnal Saintika, 15(11) : 124-131.

      [16] Harahap, F., Poerwanto, R., Suharsono, Suriani, C., & Rahayu, S. 2014. In Vitro Growth and Rooting of Mangosteen (Garnicia mangostana L.) on Medium with Different Concentration of Plant Growth Regulator. Hayati Journal of Bioscience, 21(4) : 151-158. https://doi.org/10.4308/hjb.21.4.151.

      [17] Harahap, F., Poerwanto, R., Sobir, Hasruddin, Suriani, C., Siallagan, J & Rohyana. 2015. Sterilization of Pineapple Explant From Sipahutar, North Sumatera, Indonesia (Ananas comosus L.) and In Vitro Growth Induction. Asian Jr. Of Microbiol. Biotech, 17(2) :469-478.

      [18] Heriansyah, P. 2014. Pengaruh Pemberian Myo-inositol Dan Arang Aktif Pada Media Sub Kultur Jaringan Tanaman Anggrek (Dendrobium Sp) Jurnal Agroteknologi, 5 (1) : 9 – 16

      [19] Idowu, A, E, P. Akinyemi, S, O, S. and Ibitoye, O, D. 2014. Influence of medium type and growth regulators on in vitro micropropagation of pineapple (Ananas comosus (L.), var. Smooth cayenne) African Journal of Plant Science, 8(9): 450-456.

      [20] Kartina. Nurmayulis dan Susiyanti. 2011. Pengaruh indole butiric acid (IBA) terhadap Pembentukan akar pada tanaman aren. J. Agrivigor, 10(2): 208-218.

      [21] Khan S, Nasib A, Saeed BA (2004). Employment of in vitro technology for large-scale multiplication of pineapples (Ananas comosus). Pak. J. Bot, 36(3): 611 - 615.

      [22] Keller, R., Brearley, C., Trethewey, R., and Muller-Rober, B. (1998) Reduced inositol content and altered morphology in transgenic potato plants inhibited for 1D-myoinositol 3-phosphate synthase. The Plant Journal. 16:403 - 410. https://doi.org/10.1046/j.1365-313x.1998.00309.x.

      [23] Loewus, F. and Murthy, P. (2000) Myo inositol metabolism in plants. Plant Science. Elsevier Science. 150:1-19.

      [24] Mashud, N. 2013. Efek Zat Pengatur Tumbuh BAP Terhadap Pertumbuhan Planlet Kelapa Genjah Kopyor dari Kecambah yang Dibelah Balai Penelitian Tanaman Palma, 14 (2): 82 - 87

      [25] Mulyono, D. 2010. Pengaruh Zat Pengatur Tumbuh Auksin: Indole Butiric Acid (IBA) dan Sitokinin: Benzil Amino Purine (BAP) dan Kinetin dalam Elongasi Pertunasan gaharu (Aquilaria beccariana). Jurnal Sains dan Teknologi Indonesia, 12 (2): 1 – 7.

      [26] Paramartha, I, A. Ermavitalini, D dan Nurfadilah, S. 2012. Pengaruh Penambahan Kombinasi Konsentrasi ZPT NAA dan BAP terhadap Pertumbuhan dan Perkembangan Biji Dendrobium Taurulinum J.J Smith Secara in Vitro. Jurnal Sains Dan Seni Its, 1 (1): 40 – 43.

      [27] Ramesh, Y., and V. Ramassamy. 2014. Effect of gelling agents in in vitro multiplication of banana var. Poovan. Int. J. Advanced Bio. Research 4(3): 308 - 311.

      [28] Rostiana, O. dan Seswita, D. 2007. Pengaruh Indole Butyric Acid DAN Naphtaleine Acetic Acid Terhadap Induksi Perakaran Tunas Piretrum [Chrysanthemum cinerariifolium (Trevir.)Vis.] Klon Prau 6 Secara in Vitro. Bul. Littr, 18 (1): 39 – 48.

      [29] Royani, I. Zulkifli, L. dan Sedijani P. 2015. Regenerasi Tunas Dan Akar Dari Kalus Daun Kacang Tanah (Arachis hypogaea L.) Varietas Kelinci Dengan KombinasI 2, 4-D dan BAP.

      [30] Rugayah, Anggalia I, Ginting C, Y. 2013. Pengaruh Konsentrasi Dan Cara Aplikasi IBA (Indole Butiric Acid) Terhadap Pertumbuhan Bibit Nanas (Ananas comosus [L.] Merr.) Asal Tunas Mahkota. Jurnal Agrotropika, 17(1): 35-38.

      [31] Santoso, D, R. dan Sobir. 2013 Pertumbuhan Planlet Nenas (Ananas comosus L. Merr.) Varietas Smooth Cayenne Hasil Kultur in Vitro pada Beberapa Konsentrasi BAP dan Umur Plantlet. Bul. Agrohorti, 1 (1): 54 – 61.

      [32] Sepahvand, S. Ebadi A. Kamali K. Ghaemmaghami S.A. 2012. Effects of Myo-Inositol and Thiamine on Micropropagation of GF677 (Peach × Almond Hybrid) Journal of Agricultural Science, 4 (2): 275 – 280.

      [33] Srilestari, R dan Sasmita, R, E. 2015. Perbanyakan pisang raja bulu secara in vitro dengan menggunakan pupuk daun. Agrivet, 19: 1-6.

      [34] Shofiana A, Rahayu Y, Lukas S. Budipramana. 2013. Pengaruh Pemberian Berbagai Konsentrasi Hormon IBA (Indole Butyric Acid) terhadap Pertumbuhan Akar pada Stek Batang Tanaman Buah Naga (Hylocereus undatus). LenteraBio, 2 (1):101–105.

      [35] Sitorus, N. Hastuti, E dan Setiari, N. 2011. Induksi Kalus Binahong (Basella rubra L.) Secara in Vitro Pada Media Murashige & Skoog Dengan Konsentrasi Sukrosa Yang Berbeda. BIOMA, 13 (1): 1 -7.

      [36] Smart, C.C. and Fleming, A.J. (1993) a plant gene with homology to D-myo-inositol-3-phosphate synthase is rapidly and spatially up regulated during an abscisic-acid-induced morphogenic response in Spirodela polyrrhiza. Plant J, 4: 279 - 293. https://doi.org/10.1046/j.1365-313X.1993.04020279.x.

      [37] Syafii M, Badami K, Nursandi F. 2013. Pengaruh indol-3-butiric-acid dan Thidiazuron Terhadap Multiplikasi Tunas Nenas (ananas comosus (l) merr) cv. Smooth cayyene secara in vitro. Jurnal Rekayasa, 6 (1) 6 14.

      [38] Widiastoety. A, Santi. dan N, Solvia. 2012. Pengaruh Myo-inositol dan Arang Aktif terhadap Pertumbuhan Planlet Anggrek Dendrobium dalam Kultur in Vitro. J. Hort. 22(3): 205-209 https://doi.org/10.21082/jhort.v22n3.2012.p205-209.

      [39] Yusnita. 2004. Kultur Jaringan Cara Memperbanyak Tanaman Secara Efisien. PT Agromedia pustaka.

      [40] Zulkarnain and Neliyati. 2017. The Effect of NAA and BAP on Tissue Culture of Tangkit Pineapple (Ananas comosus (L.) Merr. cv. Tangkit). Biospecies, 10 (1) : 1-10.

      [41] Wicaksono, F. Y. T. Nurmala, A.W. Irwan, A.S.U. Putri. 2016. Pengaruh pemberian gibberellin dan sitokinin pada konsentrasi yang berbeda terhadap pertumbuhan dan hasil gandum (Triticum aestivum L.) di dataran medium Jatinangor. Jurnal Kultivasi, 15(1):52 -58. https://doi.org/10.24198/kltv.v15i1.12004.

  • Downloads

  • How to Cite

    Hasanah, A., Harahap, F., & Silaban, R. (2018). The Effects of MYO-inositol and Indole Butyric Acid (IBA) on the Formation of Pineapples Root (Ananas comosus L.) from Sipahutar North Sumatera In Vitro. International Journal of Biological Research, 6(2), 23-28. https://doi.org/10.14419/ijbr.v6i2.13699