Effect of trichloroethylene (TCE) on female albino mice and their early embryos

  • Authors

    • AM El-Shershaby
    • RM El-Balshy
    • SI Abd El-Hady
    • NM Abou-Rashed Dept. of Zoology, Faculty of Science, Benha University, Egypt. cell no. 002-01286024454
    2014-04-12
    https://doi.org/10.14419/ijbr.v2i1.1946

  • Abstract

    The present study investigates the effects of different doses of "trichloroethylene" TCE on pregnancy, early embryos on days 6 and 8 of pregnancy. The experimental females (90 virgin females) were divided into three groups; the first one (C) was the control that treated with the solvent only (corn oil), the second (G1) and the third (G2) treated groups with low (24 mg/kg) and high (240 mg/kg) dose of TCE, respectively. Oral administration of TCE to female mice once daily for a period of 21 days before mating and till 5th and 7th day of pregnancy, caused a significant decrease in the body weight for non-pregnant females and weight gain for pregnant females. The percentage of survival, mating and abortion were also significantly decreased by TCE treatment for pregnant females.  Histological examination for the obtained 6 and 8 day mice embryos from treated mothers showed some histopathological alterations in compared with the normal control embryos. These alterations indicated that TCE treatment resulted in retarded, delayed and deformed embryos. The results suggest that TCE has teratogenic and embryo toxic effects on the early development of mice embryos following a short-term of exposure.

     

    Keywords: Trichloroethylene, Mating, 6th and 8th Day Mouse Embryo, Histopathology, Early Developmental Stages.

  • References

    1. ATSDR (Agency for Toxic Substances and Disease Registry). (2001), Final Report, Evaluation of priority health conditions in a community with historical contamination by trichloroethylene. Atlanta, GA: U.S. Department of Health and Human Services.
    2. ATSDR (Agency for Toxic Substances and Disease Registry). (2008), Health consultation: Health statistics review follow-up: Cancer and birth outcome analysis, Endicott area investigation, Endicott area, Town of Union, Broome County, New York. Atlanta, GA: U.S. Department of Health and Human Services.
    3. Carney E, Thorsrud B, Dugard P & Zablotny C (2006), Developmental toxicity studies in Crl:CD (SD) rats following inhalation exposure to trichloroethylene and perchloroethylene. Birth Defects Res. Dev. Reprod. Toxicol. 77, 405-412.
    4. Collins TFX, Welsh J, Black T & Collins E (1981), A study of the teratogenic potential of caffeine in rats when given by oral intubation. Regul. Toxicol. Pharmacol., 1, 355-378.
    5. Collins TFX, Welsh J, Black T & Reggles DI (1983), A study of the teratogenic potential of caffeine ingested in drinking water. Food Chem. Toxicol., 2(6), 673-777.
    6. Dallas CE, Gallo JM, Ramanathan R, Muralidhara S & Bruckner JV (1991), Physiological pharmacokinetic modeling of inhaled trichloroethylene in rats. Toxicol. Appl. Pharmacol., 110, 303-314.
    7. De-Baere S, Meyer E, Dirinck I, Lambert W, Piette M, Van- Peteghem C & De-Leenheer A (1997), Tissue distribution of trichloroethylene and its metabolites in a forensic case. Anal. Toxicol. 21, 223-227.
    8. Ebeid HM, Mollal ME & El-Rayess RA (2011), Experimental studies on the effect of lead acetate on pregnant mice and their fetuses. J. Egypt. Ger. Soc. Zool., 63 (B), 25-41.
    9. El-Sayyad HI & El-Gammal HL (2001), Effects of ethylene glycol monomethyle ether on prenatal development of rats. J. Egypt. Ger. Soc. Zool., 35(B), 59-76.
    10. El-Shershaby AM, El-Ganzuri MA & Kandeel AA (2006), Teratogenic and embryolethal effects of edifenphos during early pregnancy in mice. J. Union Arab Biol. Cairo, 25(A), 67-87.
    11. El-Shershaby AM, Kaldas SK & El-Halawany MS (1998), Teratogenic effects of warfarin on the rat embryos during early pregnancy: Histological and cytological studies. J. Egypt. Ger. Soc. Zool., 25(B), 1-24.
    12. El-Shershaby AM, El-Ganzuri MA & Kandeel AA (2006), Teratogenic and embryolethal effects of edifenphos during early pregnancy in mice. J. Union Arab Biol. Cairo, 25(A), 67-87.
    13. El-Shershaby AM & Hinchliffe JR (1974), Cell redundancy in the zona intact. Preimplantation mouse blastocyst: A light and electron microscope study of dead cells and their fate. J. Embryol. Exp. Morphol., 31(3), 646-654.
    14. El-Shershaby AM and Kaldas SK (1985), Teratogenic effect of vitamin A on early embryo of the rat: A histological study. J. Histol., 8(2), 165-174.
    15. Fernandez JG, Droz PO, Humbert BE & Caperos JR (1977), Trichloroethylene exposure simulation of uptake, excretion, and metabolism using a mathematical model. Br. J. Ind. Med., 34, 43-55.
    16. Fisher JW, Gargas ML, Allen BC & Andersen ME (1991), Physiologically based pharmacokinetic modeling with trichlo-roethylene and its metabolite, trichloroacetic acid, in the rat and mouse. Toxicol. Appl. Pharmacol., 109, 183-195.
    17. Forsberg JG & Kallen B (1968), Cell death during embryogenesis. Rev. Roum. dEmbryol. Cytol, 5(2), 91-102.
    18. Glucksmann A (1965), Cell death in normal development. Arch. Leig. Rev., 76(3), 419-437.
    19. Goeptar AR, Commandeur JM, Van-Ommen B, Van-Bladeren PJ & Vermeulen NE (1995), Metabolism and kinetics of trichloroethylene in relation to toxicity and carcinogenicity. Relevance of the mercapturic acid pathway. Chem. Res. Toxicol., 8, 3-21.
    20. Goldberg S, Lebowitz M, Graver E & Hicks S (1990), An association of human congenital cardiac malformations and drinking water contaminants. J. Am. Coll. Cardiol., 16, 155-164.
    21. Howe AM & Webster WS (1990), Exposure of pregnant rat to warfarin and vitamin K, 1: An animal model of intraventricular hemorrhage in the fetus. Teratology, 42, 413-420.
    22. IARC (International Agency for Research on Cancer). (1995), Trichloroethylene. In IARC Monograph on the Evaluation of the Carcinogenic Risk of Chemicals to Humans. 63, 75-158.
    23. Kaneko T, Wang PY & Sato A (1997), Assessment of the health effects of trichloroethylene. Ind. Health, 35, 301-324.
    24. Kent C (1998), Teratogens. In: Basics of toxicology. Eds. Jhon Wiley & Sons Inc.
    25. Lash LH, Parker J & Scott C (2000), Modes of action of trichloroethylene for kidney tumorigenesis. Environ. Health Perspect., 108(2), 225-240.
    26. Lindbohm M, Taskinen H, Sallmen M & Hemminki K (1990), Spontaneous abortions among women exposed to organic solvents. Am. J. Ind. Med., 17, 449-463.
    27. Manson J, Murphy M, Richdale N & Smith M (1984), Effects of oral exposure to trichloroethylene on female reproductive function. Toxicology, 32, 229-242.
    28. Maull EA, Cogliano VJ, Scott CS, Barton HA, Fisher JW, Greenberg M, Rhomberg L & Sorgen SP (1997), Trichloroethylene health risk assessment: A new and improved process. Drug Chem. Toxicol., 20, 427-442.
    29. Mitoma C, Steeger T, Jackson SE, Wheeler KP, Rogers JH & Milman HA (1985), Metabolic disposition study of chlorinated hydrocarbons in rats and mice. Drug Chem. Toxicol., 8, 183-194.
    30. Morris, GM (1973), An electron microscopic study of the effects of hyper vitaminosis A on the maternal and embryonic relationship of the rat at 8 days of pregnancy. J. Reprod. Fertil., 33, 451-456.
    31. Mostafa, EM (1985), Studies on some factors affecting implantation in some experimental animals. Ph.D. Thesis, Fac. Med. Assiut Univ.
    32. Potts DM (1969), The ultrastructure of egg implantation. Adv. Reprod. Physiol., 4, 241-267.
    33. Prout, MS, Provan WM & Green T (1985), Species differences in response to trichloroethylene. Pharmacokinetics in rats and mice. Toxicol. Appl. Pharmacol., 79, 389-400.
    34. Ramdhan D, Kamijima M, Wang D, Ito Y, Naito H, Yanagiba, Y, Hayashi Y, Tanaka N, Aoyama T, Gonzalez F & Nakajima T (2010), Differential response to trichloroethylene-induced hepatosteatosis in wild-type and PPARalpha-humanized mice. Environ. Health Perspect., 118, 1557-1563.
    35. Rouisse L & Chakrabarti SK (1986), Dose-dependent metabolism of trichloroethylene and its relevance to hepatotoxicity in rats. Environ. Res., 40, 450-458.
    36. Taskinen H, Kyyronen P & Hemminki K (1994), Laboratory work and pregnancy outcome. J. Occup. Med., 36, 311-319.
    37. Ugden PC & Miller JW (1983), Chlorinated acids and chloral in drinking water. J. Am. Water Works Assoc., 75, 524 -527.
    38. Windham G, Shusterman D, Swan S, Fenster L & Eskenazi B (1991), Exposure to organic solvents and adverse pregnancy outcome. Am. J. Ind. Med., 20, 241-259.

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    El-Shershaby, A., El-Balshy, R., Abd El-Hady, S., & Abou-Rashed, N. (2014). Effect of trichloroethylene (TCE) on female albino mice and their early embryos. International Journal of Biological Research, 2(1), 9-17. https://doi.org/10.14419/ijbr.v2i1.1946

    Received date: 2014-02-05

    Accepted date: 2014-03-01

    Published date: 2014-04-12