Characterization of vibrio cholerae isolated from human Diarrhoea in mymensingh, Bangladesh
-
2014-05-20 https://doi.org/10.14419/ijbr.v2i1.2493
-
Abstract
Diarrhoeal diseases are one of the top five causes of mortality in all age groups in Bangladesh. An investigation was carried out with the aim of focusing the isolation and characterization of Vibrio cholerae from diarrhoeal stools of human in Mymensingh, Bangladesh. A total of 42 rectal swab samples were aseptically collected from human. Vibrio cholerae was isolated from diarrhoeal stools of human by cultural and biochemical examinations and susceptibility of the isolated Vibrio cholerae to different antibacterial agents was performed by Disc diffusion test. Out of 42 samples, 8 were found to be positive for Vibrio cholerae. The overall prevalence of Vibrio cholerae was 19.05%. The antibiotic sensitivity pattern demonstrated that Vibrio cholerae isolates were highly sensitive to Polymixin B, ciprofloxacin and co-trimoxazole; moderately sensitive to cephalexin and nalidixic acid; less sensitive to erythromycin; and resistant to amoxicillin. This study suggested that there was remarkable prevalence of Vibrio cholerae in human diarrhoea and Polymixin B, ciprofloxacin and co-trimoxazole could be used for treating the patients with diarrhoea caused by Vibrio cholerae.
Keywords: Characterization, Vibrio Cholerae, Human, Diarrhoea.
-
References
- Albert MJ, Siddique AK, Islam MS, Faruque AS, Ansaruzzaman M, Faruque SM and Sack RB.1993, Large outbreak of clinical cholera due to Vibrio cholerae non-O1 in Bangladesh, The Lancet, 341:704.
- Azman AS, Rudolph KE, Cummings DAT, Lessler J. 2012. "The incubation period of cholera: A systematic review", Journal of Infection, 66: 432–438.
- Barrow GI and RKA Feltham. 1993, Cowan and Steel's manual for the identification of medical bacteria, 3rd ed. Cambridge University Press, Cambridge, Mel-bourne, Australia.
- Bauer AW, Kirby WM, Sherris JC, Turck M. 1966, Antibiotic susceptibility testing by a standardized single disc method, American Journal of Clinical Pathology, 45:493-496.
- Bentivoglio M and Pacini P. 1995, "Filippo Pacini: a determined observer", Brain Research Bulletin, 38: 161–165.
- Chatterjee SN, Maiti M. 1984, Vibriophages and Vibriocins: physical, chemical and biological properties, Advanced Virus Research, 29: 263-312.
- Cheesbrough M. 1985, Medical laboratory manual for tropical countries, Vol. II: Microbiology, 400-480.
- Choopun N, Louis V, Huq A and Colwell RR. 2002, Simple Procedure for Rapid Identification of Vibrio cholerae from the Aquatic Environment, Applied and Environmental Microbiology, 68: 995- 998.
- Faruque SM and Nair GB. 2008, Vibrio cholerae: Genomics and Molecular Biology, Caister Academic Press. Norfolk, UK.
- Howard-Jones N. 1984, Robert Koch and the cholera vibrio: a centenary, British Medical Journal. 288: 379–381.
- Islam MS, Drasar BS and Sack RB. 1993, The aquatic environment as a reservoir of Vibrio cholerae: a review, Journal of Diarrhoeal Disease Research, 11:197-206.
- Kaper JB, Morris JG Jr and Levine MM.1995, Cholera. Clinical Microbiology Review, 8:48-86.
- Khan AW, Hossain SJ and Uddin SN. 2007, Isolation, identification and determination of antibiotic susceptibility of Vibrio parahaemolyticus from shrimp at Khulna region of Bangladesh, Research Journal of Microbiology, 2: 216-227.
- Mandal S, Pal NK, Chowdhury IH and Debmandal M. 2009, Antibacterial activity of ciprofloxacin and trimethoprim, alone and in combinittion, against Vibrio cholerae O1 biotype El Tor serotype Ogawa isolates, Polish Journal of Microbiology, 58:57-60.
- Pedersen K, Grisez L, van Houdt R, Tiainen T, Ollevier F and Larsen JL .1999, Extended Serotyping Scheme for Vibrio anguillarum with the Definition and Characterization of Seven Provisional O-Serogroups, Current Microbiology, 38: 183-189.
- Ryan KJ and Ray CG. 2004, Sherris Medical Microbiology. 4th ed. McGraw Hill, 376– 377.
- Sack DA, Sack RB and Chaignat CL. 2006, Getting serious about cholera, The New England Journal of Medicine, 355: 649–651.
- Sack DA, Sack RB, Nair GB and Siddique AK. 2004, Cholera, Lancet, 363: 223-233.
- World Health Organization. 2006, Mortality country fact sheet 2006, Geneva: World Health Organization.
-
Downloads
-
How to Cite
Rahamat Ali, M., Shahidur Rahman Khan, M., Arifa Khatun, M., Shah Alam, M., Mostafa Anower, A., & Enamul Hoque Kayesh, M. (2014). Characterization of vibrio cholerae isolated from human Diarrhoea in mymensingh, Bangladesh. International Journal of Biological Research, 2(1), 23-25. https://doi.org/10.14419/ijbr.v2i1.2493Received date: 2014-04-19
Accepted date: 2014-05-17
Published date: 2014-05-20