Levels of IL-6 and IL-8 in complicated versus uncomplicated urinary tract infection

  • Authors

    • Maryam Alaa Ahmed Department of Medical Microbiology and Immunology, Collage of medicine, Kerbala University, 56001, Karbala, Iraq
    • Mohaned Muhsen Ahmed Department of Medical Microbiology and Immunology, Collage of medicine, Kerbala University, 56001, Karbala, Iraq
    • Ali Jaleel Ali Department of Medical Microbiology and Immunology, Collage of medicine, Kerbala University, 56001, Karbala, Iraq
    2024-10-31
    https://doi.org/10.14419/q1jngf87
  • Use about five key words or phrases in alphabetical order, Separated by Semicolon.

  • Abstract

    Background: Urinary tract infections (UTIs) are one of the world's most frequent infectious diseases, affecting 150 million people each year and resulting in severe morbidity and high medical expenditures. UTIs can be clinically defined as uncomplicated UTIs (uUTIs) or complicated UTIs (cUTIs) to differentiate infections of benign origin from those with a higher risk of recurrence or progression to severe pathology. The aim of this study was to evaluate interleukin-6 (IL-6) and IL-8 as potential biomarkers in differentiating complicated and uncomplicated

    Methods: Ninety samples of urine were collected. There were 43 samples from healthy controls and 47 samples from UTI patients, those who have been clinically diagnosed with UTIs by the urologist. The serum IL-6 and IL-8 were measured using an enzyme-linked immunosorbent assay and commercially available kits.

    Results: The levels of both IL-6 and IL-8 were significantly higher in UTI patient then in control subjects (p=0.001, p=0.001). A significant difference was observed in the mean of level IL-6 between culture positive UTI patients and culture negative UTI patients (p=0.014). In addition, the type of bacterial growth, a significant difference was observed in the mean of serum IL-6 and IL-8 level in UTI caused by gram negative bacteria in comparison with UTI caused by gram positive bacteria.

    Conclusion: High levels of IL-6 are associated with the growth of gram-negative bacteria, and this indicates that gram-negative bacteria could induce stronger inflammatory responses than gram-positive bacteria. IL-8 levels are higher in UTIs caused by gram-negative bacteria. This result further supported the evidence that gram-negative bacteria are potent inducers of inflammatory responses.

  • References

    1. Abbas, S., Mahdi, N., & Ahmed, K. (2022). Blood Groups, IL-6, IL-8 and HS-CRP Levels in Non-Pregnant Women With Urinary Tract Infection Caused by Escherichia coli. INTERNATIONAL JOURNAL OF MEDICAL SCIENCES, 5(2), 33-43.
    2. Al-Kaabi, H. K. J., & Al-Khalidi, B. A. H. (2020). Investigation of IL-6, IL-8 and TNF-α among patients infected with Proteus mirabilis in UTI Cases. Journal of Physics: Conference Series,
    3. Al Rushood, M., Al-Eisa, A., & AL-Attiyah, R. (2020). Serum and urine interleukin-6 and interleukin-8 levels do not differentiate acute pyelonephritis from lower urinary tract infections in children. Journal of Inflammation Research, 789-797. https://doi.org/10.2147/JIR.S275570.
    4. Codelia-Anjum, A., Lerner, L. B., Elterman, D., Zorn, K. C., Bhojani, N., & Chughtai, B. (2023). Enterococcal Urinary Tract Infections: A Review of the Pathogenicity, Epidemiology, and Treatment. Antibiotics, 12(4), 778. https://doi.org/10.3390/antibiotics12040778.
    5. Darogha, S. N., Azeez, S. H., & Abdullah, Z. G. (2021). Evaluation of procalcitonin and interleukin-6 as a marker of bacterial urinary tract infection. Cellular and Molecular Biology, 67(4), 203-213. https://doi.org/10.14715/cmb/2021.67.4.23.
    6. Flores-Mireles, A. L., Walker, J. N., Caparon, M., & Hultgren, S. J. (2015). Urinary tract infections: epidemiology, mechanisms of infection and treatment options. Nature Reviews Microbiology, 13(5), 269-284. https://doi.org/10.1038/nrmicro3432.
    7. Gokce, I., Alpay, H., Biyikli, N., Unluguzel, G., Dede, F., & Topuzoglu, A. (2010). Urinary levels of interleukin-6 and interleukin-8 in patients with vesicoureteral reflux and renal parenchymal scar. Pediatric Nephrology, 25, 905-912. https://doi.org/10.1007/s00467-009-1396-2.
    8. González de Llano, D., Moreno-Arribas, M. V., & Bartolomé, B. (2020). Cranberry polyphenols and prevention against urinary tract infections: relevant considerations. Molecules, 25(15), 3523. https://doi.org/10.3390/molecules25153523.
    9. Jagtap, S., Harikumar, S., Vinayagamoorthy, V., Mukhopadhyay, S., & Dongre, A. (2022). Comprehensive assessment of holding urine as a behavioral risk factor for UTI in women and reasons for delayed voiding. BMC Infect Dis, 22(1), 521. https://doi.org/10.1186/s12879-022-07501-4.
    10. Mahyar, A., Ayazi, P., Yarigarravesh, M. H., Khoeiniha, M. H., Oveisi, S., Sahmani, A. A., & Esmaeily, S. (2015). Serum interleukin -8 is not a reliable marker for prediction of vesicoureteral reflux in children with febrile urinary tract infection. Int Braz J Urol, 41(6), 1160-1166. https://doi.org/10.1590/S1677-5538.IBJU.2014.0381.
    11. Maisto, M., Iannuzzo, F., Novellino, E., Schiano, E., Piccolo, V., & Tenore, G. C. (2023). Natural Polyphenols for Prevention and Treatment of Urinary Tract Infections. International journal of molecular sciences, 24(4), 3277. https://doi.org/10.3390/ijms24043277.
    12. McLellan, L. K., & Hunstad, D. A. (2016). Urinary tract infection: pathogenesis and outlook. Trends in molecular medicine, 22(11), 946-957. https://doi.org/10.1016/j.molmed.2016.09.003.
    13. Pirdel, L., & Pirdel, M. (2021). A Case-control Study of Urinary Tract Infection, 25‑hydroxyvitamin D Status and Associated Inflammatory and Regulatory Responses. Research in Molecular Medicine, 9(1), 51-60. https://doi.org/10.32598/rmm.9.1.6.
    14. Ragnarsdóttir, B., & Svanborg, C. (2012). Susceptibility to acute pyelonephritis or asymptomatic bacteriuria: host–pathogen interaction in urinary tract infections. Pediatric Nephrology, 27, 2017-2029. https://doi.org/10.1007/s00467-011-2089-1.
    15. Wojdasiewicz, P., Poniatowski, Ł. A., & Szukiewicz, D. (2014). The role of inflammatory and anti-inflammatory cytokines in the path-ogenesis of osteoarthritis. Mediators of inflammation, 2014. https://doi.org/10.1155/2014/561459.

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  • How to Cite

    Ahmed, M. A., Ahmed, M. M., & Ali, A. J. (2024). Levels of IL-6 and IL-8 in complicated versus uncomplicated urinary tract infection. International Journal of Biological Research, 11(2), 75-78. https://doi.org/10.14419/q1jngf87