A comparative evaluation of the inulin utilization potential of two Bifidobacterium animalis subsp. lactis strains for the development of a synbiotic powder

  • Authors

    • LIGIMOL JAMES Department of Dairy Microbiology, College of Dairy Science and Technology, Mannuthy, thrissur
    • Anil K. Puniya
    • Kishan Singh
    2014-09-16
    https://doi.org/10.14419/ijbr.v2i2.3314

  • Assessment of the compatibility between the probiotic and prebiotic is identified as a critical step while developing synbiotic products. So a study was conducted to compare the inulin utilization potential of two Bifidobacterium animalis subsp. lactis strains for selecting a strain for the development of a lyophilized synbiotic product. The growth stimulatory effect of inulin was found to be more prominent on Bifidobacterium animalis subsp. lactis B420 than on Bifidobacterium animalis subsp. lactis Bb-12. So a synbiotic pair of Bifidobacterium animalis subsp. lactis B420 and inulin was selected for the development of the synbiotic product. The developed product had a high probiotic count of  6.5x108 cfu/g, showing a survival rate of 65%. On assessing the probiotic count of the developed product during storage, a marked reduction was observed on room temperature storage with the counts reducing to the level of less than five log cfu/g after 60 days. In contrast the count remained in the range of eight to nine log cfu/g even after 60 days of refrigerated storage. So it could be a suggested that considering the probiotic stability and viability problems pertaining to fermented dairy products, lyophilized products could be potential alternatives as matrices for the delivery of probiotics to humans.

    Keywords: Bifidobacterium animalis subsp. lactis Bb-12, Bifidobacterium animalis subsp. lactis B420, Inulin, Lyophilized powder, Synbiotics.

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  • How to Cite

    JAMES, L., Puniya, A. K., & Singh, K. (2014). A comparative evaluation of the inulin utilization potential of two Bifidobacterium animalis subsp. lactis strains for the development of a synbiotic powder. International Journal of Biological Research, 2(2), 121-124. https://doi.org/10.14419/ijbr.v2i2.3314