Studies on the antimicrobial potency of the marine algae - Caulerpa scalpelliformis and Spyridia species
-
2014-10-07 https://doi.org/10.14419/ijbr.v2i2.3515 -
Abstract
Objective: To study the antimicrobial potency of the marine algae – Caulerpa scalpelliformis and Spyridia sp.
Methods: Caulerpa scalpelliformis and Spyridia sp. were screened for the presence of bioactive components by extracting them with suitable solvents and studying their antimicrobial activities against varied human pathogens. An attempt has also been made to separate the unknown compounds using Thin Layer Chromatography and purify the extracts using column chromatography since not much work has been carried out in these species.
Results: In Spyridia, only the methanolic extract could produce zone of inhibition against Staphylococcus aureus and Salmonella sp. whereas in Caulerpa scalpelliformis, only the petroleum ether, chloroform and methanolic extracts showed antimicrobial properties. Better resolution was found in the mobile phase containing chloroform: methanol 80: 20 ratio for Caulerpa. Hence, its methanolic extract was fractioned in silica gel column and these fractions were effective against some of the test organ-isms but not against all.
Conclusion: Further investigation is required to study the nature of compounds with antimicrobial properties with reference to Caulerpa scalpelliformis; whereas with Spyridia sp. the study can be extracted to collect the samples during different seasons of a year and confirm its antimicrobial potency.
Keywords: Caulerpa Scalpelliformis, Spyridia SP, Antimicrobial Activities, Thin Layer Chromatography, Column Chromatography.
-
References
- Abbott, I.A., Fisher, J. Mc Dermid, K.J. (2002). New re-ported and revised marine algae from the Nha Trang,Vietnam, in: taxonomy of economic seaweed with reference to some pacific species. Oceanographic Institute Nha Trang Vietnam 8:291-321
- Harvey SC, J Luo & R Lavery. (1988). DNA stem-loop structures in oligopurine-oligopyrimidine triplexes. Nucleic Acids Research 16(24): 11795-11809. http://dx.doi.org/10.1093/nar/16.24.11795.
- González F & M Silva. (2001). Biodiversidad química de macroalgas marinas. In: Alveal K & T Antezana (eds). Sustentabilidad de la biodiversidad, unproblema actual. Bases científico-técnicas, teorizaciones y proyecciones, and pp. 415-496. Universidad de Concepción, Concepción.
- Pereira R.C., B.A.P. DaGama, V.L. Teixeira and Y. Yoneshigue-Valentin (2003). Ecological roles of natural products of the Brazilian red seaweed Laurencia obtuse. Braz.J.Biol 63(4):665-672. http://dx.doi.org/10.1590/S1519-69842003000400013.
- Bauer,A.W., W.M.Kirby, J.C.Scheris and M.Truck (1966). Antibiotic susceptibility testing by standardization by single disc method.Amer.J.Clinical Pathol 45:493-496.
- Vairappan C. S., Suzuki, M., Abe T., Masuda, M. (2001). Halogenated metabolites with antibacterial activity from the Okinawa, Laurencia sp phytochemistry 58: 517-523.
- Cragg, G. M., Newman and K. M. Snader, (1997). Natural products in drug discovery and development J.Nat prod 60: 52-60. http://dx.doi.org/10.1021/np9604893.
-
Downloads
-
How to Cite
Singh, M. (2014). Studies on the antimicrobial potency of the marine algae - Caulerpa scalpelliformis and Spyridia species. International Journal of Biological Research, 2(2), 131-133. https://doi.org/10.14419/ijbr.v2i2.3515Received date: 2014-09-02
Accepted date: 2014-09-28
Published date: 2014-10-07