Characterization of an alkaline protease with high quality bating potential in leather processing from Bacillus licheniformis MZK05M9 mutant

  • Authors

    • Md. Arafat Al Mamun Centre for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka-1000, Bangladesh.
    • Md. Murad Khan Department of Microbiology, University of Dhaka, Dhaka-1000, Bangladesh
    • Md. Nahinur Rahmam Akand Department of Microbiology, University of Dhaka, Dhaka-1000, Bangladesh
    • Shakila Nargis Khan Department of Microbiology, University of Dhaka, Dhaka-1000, Bangladesh
    • Md. Mozammel Hoq Department of Microbiology, University of Dhaka, Dhaka-1000, Bangladesh
    2015-03-24
    https://doi.org/10.14419/ijbr.v3i1.4250
  • Alkaline Protease, Bacillus licheniformis MZK05M9 (BlM9), Bating, Characterization, Eco-Friendly.

  • Abstract

    An alkaline protease from Bacillus licheniformis MZK05M9 (BlM9), a mutant strain developed in our laboratory, has been partially purified and characterized for its robustness and eco-friendly application potential in processing of hides and skins for leather manufacturing and detergent industries. The enzyme was purified 2.70 fold with specific activity of 1624U/mg in comparison to crude enzyme extract by using ammonium sulfate precipitation, dialysis and Sephadex G-75 column chromatography. The molecular mass of the enzyme was 27.2 kDa as judged by SDS–PAGE. The purified protease had a pH optimum of 8.5 and temperature optimum of 55°C. According to the inhibition profiles obtained with the various protease inhibitors, it was confirmed that the partially purified protease belongs to the serine protease type. The activity of partially purified enzyme was enhanced by calcium, magnesium, barium, potassium and manganese ions and strongly inhibited by mercury ion. In addition, the protease showed remarkable stability in the presence of 1% SDS; 1, 3 and 5% Triton X-100 and H2O2, which comprise the common bleach-based detergent formulation. The enzyme was found equally efficient to a commercial enzyme Oropon K (one of the commercial enzymes imported into Bangladesh for bating purpose) in bating of animal hide as proved by different comparative qualitative tests such as tensile strength, percent of elongation, stitch tears strength, water vapor permeability, grain crack strength and tongue tear  strength tests. In addition, the stability profile (pH, temperature and surfactants) and blood stain removal data also revealed its suitability for application in detergent industry.

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  • How to Cite

    Mamun, M. A. A., Khan, M. M., Akand, M. N. R., Khan, S. N., & Hoq, M. M. (2015). Characterization of an alkaline protease with high quality bating potential in leather processing from Bacillus licheniformis MZK05M9 mutant. International Journal of Biological Research, 3(1), 36-41. https://doi.org/10.14419/ijbr.v3i1.4250

    Received date: 2015-01-31

    Accepted date: 2015-02-23

    Published date: 2015-03-24