In vitro meristem-tip culture and regeneration approaches in Congolese cassava accessions (Manihot esculenta Crantz cv. Boma and cv. Mpelo-Nlongi)

  • Authors

    • J.-Roger Bansimba Mukiese Institut Supérieur Pédagogique de Mbanza-Ngungu
    • Aimé Diamuini Ndofunsu
    • Freddy Bulubulu
    • Alexandre Mbaya Ntumbula
    • Sébastien Luyindula Ndiku
    2015-11-05
    https://doi.org/10.14419/ijbr.v3i2.5197
  • Vitroplant, Phytohormone, Mpelo-Nlongi, Boma, Callus, Naphtalenacetic Acid, Benzylaminopurine, Gibberellic Acid.
  • Abstract

    Shiny dome-like structures measuring less than 1mm in length were excised aseptically from shoot tip buds of infected of two cassava (Manihot esculenta Crantz) local cultivars (Boma and Mpelo Nlongi) and cultivated in vitro in two types of media with different combination of growth hormone: Murashige and Skoog supplemented of sucrose (20 g/l), Naphtalenacetic acid (NAA, 10 μM), Ben-zylaminopurine (BAP, 0.66 μM) as well as Gibberellic acid (GA3, 0.1 μM) with 80 mg/l of Adenine sulphate and MS-free growth regulators. After four weeks, data were scored: 29.5% responding explant with callus formation and 20.5% responding explants to shoot development in the medium with growth regulators for the cultivar Boma whereas the cultivar Mpelo-Nlongi presented 5.7% and 25.7% respectively of callus formation and shoot development. The cultivar Boma presented a tendency more pronounced for the callus formation rather than with the shoot development contrary to the cultivar Mpelo-Nlongi. In regards of this experiment, it was shown that the media composition and genotype are essential factors, which influence in vitro growth, mainly the shoot development, in the culture of meristems for cassava local accessions.

  • References

    1. [1] Bansimba Mukiese JR (2015) A cassava mosaic disease epidemiologic factors analysis in the Bas-Congo (DR Congo) zone of savannah. Case study: «Secteur de Boko». International Journal of Biological Research, 3(1): 56-63. doi:10.14419/ijbr.v3il.4069.

      [2] Bock KR (1988) Quelques aspects de l’épidémiologie de la mosaïque africaine du manioc dans les régions côtières du Kenya. Maladies des Plantes 76 :115-120.

      [3] International Institute of Tropical Agriculture (1990) Cassava in tropical Africa. A reference manual

      [4] Kartha KK, Pahl K, Leung NL & Mroginski LA (1981) Plant regeneration from meristems of grain legumes: soybean, cowpea, peanut, chickpea and bean. Can. J. Bot. 59: 1671-1679. http://dx.doi.org/10.1139/b81-225.

      [5] Kartha KK and Gamborg OL (1975) Elimination of Cassava Mosaic Disease by meristem culture. Phytopathology 65:826-828. http://dx.doi.org/10.1094/Phyto-65-826.

      [6] Kawuki RS, Pariyo A, Amuge T, Nuwamanya E, Ssemakula G, Tumwesigye S, Bua A, Baguma Y, Omongo C, Alicai T & Orone J (2011) A breeding scheme for local adoption of cassava (Manihot esculenta Crantz). Journal of Plant Breeding and Crop Science 3(7): 120-130. ISSN 2006-9758. (http://academicjournals.org/jpbcs).

      [7] Malaurie B, Okito P & Trouslot M-F (1995) Effect of growth regulators concentrations on morphological developement of meristem-tips in Dioscorea cayenensis-D. rotundata complex and D. praehensilis. Plant Cell, Tissue and Organ Culture 41(3):229-235. http://dx.doi.org/10.1007/BF00045086.

      [8] Mallowa SO, Isutsa DK, Kamau AW & Legg JP, (2006) Effectiveness of phytosanitation in Cassava Mosaic Disease management in a post-epidemic area of western Kenya. ARPN Journal of Agricultural and Biological Science 6(7): 8-15. ISSN 1990-6145.

      [9] Mallowa SO, Isutsa DK, Kamau AW, Obonyo R & Legg JP (2006) Current characteristics of cassava mosaic disease in post-epidemic areas increase the range of possible management options. Annals of Applied Biology 0: 1-8. ISSN 0003-4746.

      [10] Manosh KB, Hossain M and Islam R (2007) Virus-free plantlets of strawberry through meristem culture. World Journal of Agricultural Sciences 3(6): 757-763. ISSN 1817-3047.

      [11] Nitsch JP & Lance-Nougarede A (1967) L’action conjuguée des auxines et des cytokinines sur les cellules de moelle de tabac: Etude physiologique et microscopie électronique. Bull. Soc. Fr. Physiol. Veg. 13: 81-118.

      [12] Opabode JT and Adebooye OC (2005) Application of biotechnology for the improvement of Nigerian indigenous leaf vegetables. African Journal of Biotechnology 4(3): 138-142.

      [13] Soyode FO and Oyetunji OJ (2009) Use of morphological characters to identify cassava mosaic disease and cassava bacterial blight resistance. African Crop Science Journal, 17: 25-39.

      [14] Tresh JM and Cooter RJ (2005) Strategies for controlling cassava mosaic virus disease in Africa. Plant Pathology 54: 587-614. http://dx.doi.org/10.1111/j.1365-3059.2005.01282.x.

      [15] Wasswa P, Alicai T & Mukasa SB (2010) Optimization of in vitro techniques for Cassava Brown Streak Virus elimination from infected cassava clones. African Crop Science Journal 18(4): 235-241. ISSN 1021-9730.

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  • How to Cite

    Mukiese, J.-R. B., Ndofunsu, A. D., Bulubulu, F., Ntumbula, A. M., & Ndiku, S. L. (2015). In vitro meristem-tip culture and regeneration approaches in Congolese cassava accessions (Manihot esculenta Crantz cv. Boma and cv. Mpelo-Nlongi). International Journal of Biological Research, 3(2), 72-75. https://doi.org/10.14419/ijbr.v3i2.5197

    Received date: 2015-08-18

    Accepted date: 2015-09-07

    Published date: 2015-11-05