Impact of the foraging activity of Apis mellifera adansonii Latreille (Hymenoptera: Apidae) and Bradyrhizobium fertilizer on pollination and yield components of Glycine max L. (Fabaceae) in the field

  • Authors

    • Stephanie Beaudelaine Kengni University of Maroua-Cameroon
    • Fernand-Nestor Tchuenguem Fohouo University of Ngaoundere-Cameroon
    • Albert Ngakou University of Ngaoundere-Cameroon
    2015-10-27
    https://doi.org/10.14419/ijbr.v3i2.5211
  • Apis mellifera adansonii, Bradyrhizobium, Foraging activity, Glycine max, Yields.

  • To determine the impact of Apis mellifera adansonii and Bradyrhizobium on pod and seed yields of Glycine max, field trials were carried out during 2012 and 2013 cropping seasons. Hence, 120 to 25658 flowers were labeled each year and divided into five treatments, differentiated according to whether plots were inoculated with Bradyrhizobium or not, or plants were protected from insects activities or not and the last treatment with flowers isolated then opened only to A. m. adansonii. The effects of Bradyrhizobium on nodulation, plant biomass and seed yield, as well as the foraging behavior of A. m. adansonii on flowers, the number of seeds per pod and the normal seeds' rate were evaluated. Results indicate that Bradyrhizobium significantly increased the number of flowers (P<0.001), root nodules (P<0.0001), plant biomass (P<0.0001), pod and seeds yields in inoculated plots. A. m. adansonii foraged on G. max flowers from 09.00 a.m. to 16.00 p.m. and throughout the whole blooming period. This insect intensely harvested only nectar. By comparing the yields of unprotected flowers to those of flowers isolated then opened to A. m. adansonii, 35.85% increase fructification index, and 73.09% increase in the number of seeds per pod due to this bee were recorded. The synergistic activity of insects and Bradyrhizobium increased the number of seeds per pod by 32.16% and the percentage of normal seeds by 32.87%. Our results reveal that inoculation of soybean plant at sowing with Bradyrhizobium and installation of hives close to the field could be recommended for a sustainable pods and seed yield improvement of this crop.

  • References

    1. [1] Ahrent DK & Caviness CE (1994) Natural cross-pollination of twelve soybean cultivars in Arkansas. Crop Science 34(2): 376-378. http://dx.doi.org/10.2135/cropsci1994.0011183X003400020013x.

      [2] Alghamdi SS (2004) Yield stability of some soybean genotypes across diverse environments. Pakistan Journal of Biological Sciences 7(12): 2109-2114.

      http://dx.doi.org/10.3923/pjbs.2004.2109.2114.

      [3] Basualdo M, Bedascarrasbure E & De Jong D (2000) Africanized honey bees (Hymenoptera: Apidae) have a greater fidelity to sunflowers than European bees. Journal of EconomicEntomology 92(2): 304-307. http://dx.doi.org/10.1603/0022-0493-93.2.304.

      [4] Boyeldieu J (1991) Produire des grains oléagineux et proteagineux. Ed. tec et Doc/Lavoisier Col. Agriculture d’aujourd’hui. pp. 115-152.

      [5] Chiari WC, Arnaut de Toledo V de A, Ruvolo-Takasusuki MCC, Braz de Oliveira AJ, Sakaguti1 ES, Attencia VM, Costa FM & Mitsui M H (2005a) Pollination of Soybean (Glycine max L. Merril) by Honeybees (Apis mellifera L.). Brazilian Archives of Biology and Technology 48(1): 31-36. http://dx.doi.org/10.1590/S1516-89132005000100005.

      [6] Denison RF & Kiers ET (2004) Why are most rhizobia beneficial to their plant hosts, 1088 rather than parasitic. Microbes Infection 6: 1235-1239 http://dx.doi.org/10.1016/j.micinf.2004.08.005.

      [7] Diers BW, Keim P, Fehr WR & Shoemaker FC (1992) RFLP analysis of Soybean seed protein and oil content. Theorical and Applied Genetics 83: 608-612. http://dx.doi.org/10.1007/bf00226905.

      [8] Douka C & Tchuenguem FF-N (2013) Foraging and pollination behavior of Apis mellifera adansonii L. (Hymenoptera, Apidae) on Phaseolus vulgaris (Fabaceae) flowers at Maroua (Cameroon). International Research Journal of Plant Science 4(2): 45-54.

      [9] Gallais A. & Bannerot H (1992) Amélioration des espèces végétales cultivées. INRA, Paris. 768 p.

      [10] Hungria M, Campo RJ & Chueire LMO (2001) Symbiotic effectiveness of fast-growing rhizobia strains isolated from soybean nodules in Brazil. Biology and Fertility of Soils 33: 387-394 http://dx.doi.org/10.1007/s003740100338.

      [11] Hymowitz T. & Harlan JR (1983) Introduction of soybean to North America by Samuel Bowen in 1765. Economic Botany 37(4): 371-379. http://dx.doi.org/10.1007/BF02904196.

      [12] Ikeogu UN & Nwofia GE (2013) Yield parameters and stability of soybean (Glycine max L. Merill) as influenced by phosphorus fertilizer rates in two ultisols. Journal of Plant Breeding and Crop Science 5(4): 54-63. http://dx.doi.org/10.5897/JPBCS12.014.

      [13] Kengni BS, Tchuenguem FF-N & Ngakou a (2015) Pollination and yield attributes of (cowpea) Vigna unguiculataL. Walp. (Fabaceae) as influenced by the foraging activity of Xylocopa olivacea Fabricius (Hymenoptera: Apidae) and inoculation with Rhizobium in Ngaoundere, Cameroon. International Journal of Agronomy and Agricultural Research 6(2): 62-76.

      [14] Kiers ET, Rousseau RA, West SA & Denison RF (2003) Host sanctions and the legume-Rhizobium mutualism. Nature 425: 78-81.http://dx.doi.org/10.1038/nature01931.

      [15] Kingha BMT, Tchuenguem FF-N, Ngakou A & Brückner D (2012) Foraging and pollination activities of Xylocopa olivacea (Hymenoptera, Apidae) on Phaseolus vulgaris (Fabaceae) flowers at Dang (Ngaoundere-Cameroon). Journal of Agricultural Extension and Rural Development 4: 330-339.

      [16] Milfont MO, Rocha EEM, Lima AON & Freitas BM (2013) Higher soybean production using honeybee and wild pollinators, a sustainable alternative to pesticides and autopollination. Environmental and Chemestry Letters 11(4): 335-341.

      http://dx.doi.org/10.1007/s10311-013-03.

      [17] MINADER / DESA (2012) Annuaire des Statistiques du secteur Agricole, Campagnes 2009 & 2010. Direction des Enquêtes et des Statistiques Agricoles. AGRI-STAT N° 17: 123 p.

      [18] Ngakou A, Nwaga D, Ntonifor NN, Tamo M, Nebane CLN & Parh IA (2007) Contribution of Arbuscular Mycorrhizal Fungi (AMF), rhizobia and Metarhizium anisopliae to cowpea production in Cameroon. International Journal of Agricultural Research 2(9): 754-764.http://dx.doi.org/10.3923/ijar.2007.754.764.

      [19] Ngakou A, Megueni C, Makalao M, Nwaga D, Taine J & Ndjouenkeu R (2008) Changes in the physico-chemical properties of soil and harvested soybean seeds in response to soil solarization and bradyrhizobial inoculation. Archives of Agronomy and Soil Science 54(2): 189-202.

      http://dx.doi.org/10.1080/03650340701793579.

      [20] Ngakou A, Megueni C, Ousseini H. & Massai A (2009) Study on the isolation and characterization of rhizobia strains as biofertilizer tools or growth improvement of four grain legumes in Cameroon. International Journal of Biological and Chemical Sciences 3(5): 1078-1089.

      [21] Ormeño-Orrillo E, Menna P, Almeida LGP, Ollero FJ, Nicolás MF, Rodrigues EP, Nakatami AS, Batista JSS, Chueire LMO, Souza RC, Vasconcelos ATR, Megías M, Hungria M & Martínez-Romero E (2012) Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean (Phaseolus vulgaris L.). BMC Genomics 13: 735

      http://dx.doi.org/10.1186/1471-2164-13-735.

      [22] Rortais A, Arnold G, Halm M-P & Touet-Briens F (2005) Modes of honeybees exposure to systemic insecticides: estimated amounts of contamined pollen and nectar consumed by different categories of bees. Apidologie 36(1): 71-83.

      http://dx.doi.org/10.1051/apido:2004071.

      [23] Summers MC & Mondor EB (2011) Rhizobium alter inducible defenses in broad bean, Vicia faba. Open journal of Ecology 1(3): 52-62.

      [24] Tamiru S, Lalit MP & Tsige A (2012) Effects of inoculation by Bradyrhizobium japonicum strains on nodulation, nitrogen fixation, and yield of soybean (Glycine max L. Merill) varieties on nitisols of Bako, Western Ethiopia. International Scholarly Research Network (2012): 1-8.

      [25] Tchuenguem FF-N, Ngakou A & Kengni BS, (2009b) Pollination and yield responses of cowpea (Vigna unguiculata L. Walp.) to the foraging activity of Apis mellifera adansonii (Hymenoptera: Apidae) at Ngaoundéré (Cameroon). African Journal of Biotechnology 8: 1988-1996.

      [26] Tchuenguem FF-N & Dounia (2014) Foraging and pollination behavior of Apis mellifera adansonii (Hymenoptera: Apidae) on Glycine max L. flowers at Maroua. Journal of Research in Biology 4(1): 1209-1219.

      [27] Tchuenguem FF-N, Djongwangwé D, Messi J & Brückner D, (2007) Exploitation des fleurs de Entada africana, Eucalalyptus camadulensis, Psidium guajava et Trichilia emetica par Apis mellifera adansonii à Dang (Ngaoundéré-Cameroun). Cameroon Journal of Experimental Biology 3: 50-60.

      [28] Thiagarajan TR & Ahamad MH, (1993) Influence of a vesicular-arbuscular mycorrhizal funguson on the competition ability of Bradyrhizobium spp. for nodulation of cowpea (Vigna unguiculata L. Walp.) in non-sterilized soil. Biology and Fertility of Soils15: 294-296.http://dx.doi.org/10.1007/BF00337215.

      [29] Tien HH, Hien TM, Son MT & Herridge D (2002) Rhizobial Inoculation and N2 fixation of soybean and mungbean in the eastern region of South Vietnam. In "inoculants and nitrogen fixation of legumes in Vietnam". Edited by D. Herridge. ACIAR Proceedings 109e.

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    Kengni, S. B., Tchuenguem Fohouo, F.-N., & Ngakou, A. (2015). Impact of the foraging activity of Apis mellifera adansonii Latreille (Hymenoptera: Apidae) and Bradyrhizobium fertilizer on pollination and yield components of Glycine max L. (Fabaceae) in the field. International Journal of Biological Research, 3(2), 64-71. https://doi.org/10.14419/ijbr.v3i2.5211