Response surface methodology for optimal immobilization of Aspergillus niger ATCC 1015 lipase by adsorption method

  • Abstract
  • Keywords
  • References
  • PDF
  • Abstract

    Optimization of Vegetable Sponge (Luffa aegyptiaca) (VS) - immobilization conditions of Aspergillus niger ATCC 1015 lipase on Solid State Fermentation (SSF) was carried out using Response Surface Methodology (RSM). Four independent variables (temperature, pH, enzyme loading and enzyme stability) were optimized using Central Composite Design of RSM for lipase production in a solid rice bran-physic nut cake medium. The optimal immobilization conditions obtained were 45 °C, pH 7.0, 2.5% (w/v) enzyme loading and 32.5% (v/v) enzyme stability (using glutaraldehyde as crosslinking agent) resulted into lipase activity of 98.6 Ug-1. The result demonstrates the potential application of vegetable sponge under SSF system in immobilizing lipase, thus contributed to efficiency of the use of this biomatrix as an immobilizing agent. The statistical tools employed predicted the optimal conditions for the production of the immobilized lipase thus revealing the full potential of the support.

  • Keywords

    Adsorption; Aspergillus niger ATCC 1015; Lipase; Immobilization; Optimization; Response Surface Methodology.

  • References

      [1] Acıkel U, Ersan M & SağAcıkel Y (2010) Optimization of critical medium components using response surface methodology for lipase production by Rhizopus delemar. Food Bioproduct Process 88, 31–39.

      [2] Afshin E, Zaliha RN, Rahman RA, Ch'ng DHE, Basri M & Salle AB (2008) a modelling study by response surface methodology and artificial neural network on culture parameters optimization for thermostable lipase production from a newly isolated thermophilic Geobacillus sp. strain ARM. BMC Biotechnology 96, 8.

      [3] Arai S, Nakashima K, Tanino T, Ogino C, Kondo A & Fukuda H (2011) Production of biodiesel fuel from soybean oil catalyzed by fungus whole-cell biocatalysts in ionic liquids. Enzyme Microbial Technology 46, 51–55.

      [4] Baharum SN, Razak ABS, Basri MC, Rahman MBA & Rahman RNZRA(2003) Organic solvent tolerant lipase by Pseudomonas sp. Strain S5: stability of enzyme in organic solvent and physical factors affecting its production. An Microbiology 53, 75-83.

      [5] Benjamin S & Pandey A, (1998) Candida rugosa lipases: Molecular biology and versatility in biotechnology, Yea. 14, 1069-1087.<1069::AID-YEA303>3.0.CO;2-K.

      [6] Bussamara R, Dall’Agno L, Schrank, A, Fernandes KF & Vainstein MH (2012) Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology,” Enzyme Research doi:10.1155/2012/329178.

      [7] Chang SW, Shaw JF, Yang KH, Chang SF & Shieh CJ (2008) Studies of optimum conditions for covalent immobilization of Candida rugosa lipase on poly (γ-glutamic acid) by RSM. Bioresource Technology 99 (8), 2800–2805.

      [8] De Souza JS, Cavalcanti-Olivera ED, Aranda DAG & Freire DMG (2010) Application of lipase from the physic nut (Jatropha curcas L.) to a new hybrid (enzyme/chemical) hydroesterification process for biodiesel production. Journal of Molecular Catalyst B: Enzyme 65, 133-137.

      [9] Deng HT, Xu ZK, Huang XJ, Wu J & Seta P (2004) Adsorption and activity of Candida rugosa lipase on polypropylenen hollow fiber membrane modified with phospholipid analogous polymers. Langmuir. 20, 23, 10168–10173.

      [10] Design Expert (2014) Stat-Ease Software version 9.0.3 Statistics Made Easy 2021 East Hennepin Ave, Suite 480 Minneapolis, MN 55413.

      [11] Dizge N, Keskinler B & Tanriseven A. (2008) Covalent attachment of microbial lipase onto microporous styrene-divinylbenzene copolymer by means of polyglutaraldehyde. Colloids & Surfaces B. 66 1, 34–38.

      [12] Gupta N, Sahai V & Gupta R (2007) Alkaline lipase from a novel strain Burkholderia biodiesel fuel from soybean oil catalyzed by fungus whole-cell biocatalysts in ionic liquids. Enzyme Microbial Technology 46, 51–55.

      [13] Lee DH, Park CH, Yeo JM & Kim SW (2006) Lipase immobilization on silica gel using a cross-linking method. Journal of Industrial and Engineering Chemistry 12 (5) 777-782.

      [14] Lin HH, Nway O, Thu WN & Mya O (2008) Screening of lipase producing yeast for lipase catalysed transesterification of vegetables oils. GMSARN International Conference on sustainable Development. Issues on Prospective GMS.

      [15] Liu CH & Chang JS (2008) Lipolytic activity of suspended and membrane immobilized lipase originating from indigenous Burkholderia sp. C20. Bioresource Technology 99 (6), 1616–1622.

      [16] Manohar B & Divakar S (2004) Applications of surface plots and statistical design to selected lipase catalyzed esterification reactions. Process Biochemistry 39, 847-853.

      [17] Mateo C, Palomo JM, Fernandez-Lorente G, Guisan JM & Fernandez-Lafuente R (2007) Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme Microbial Technology 40 (6), 1451– 1463.

      [18] McNeil GP, Shimizu S & Yamanae T, (1991) High-yield enzymatic glycerolysis of fats and oils. Journal of American Oil Chemical Society 68, 1-5.

      [19] Morais RR, Pascoal AM, Caramori SS, Lopes FM & Fernandes KF (2013) Immobilization of amylase onto Luffa operculata Fibers. Enzyme Research Article ID 803415,

      [20] Muralidhar RV, Chirumamila RR, Marchant R & Nigam P, (2001) A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources, Biochemical Engineering Journal 9, 17–23.

      [21] Onwuka GI (2005) Food Analysis and Instrumentation. Naphthali Print. A Division of HG Support Nigeria Ltd. 63-84.

      [22] Osho MB (2003) Production of biodiesel from Jatropha curcas seed oil by free and immobilized lipase of Aspergillus niger ATCC 1015. PhD Thesis Federal University of Agriculture, Nigeria, 55.

      [23] Osho MB, Akpan I & Adio OQ (2015) Screening, optimization and characterization of extracellular lipase of Aspergillus niger ATCC 1015. Journal of Microbiology Biotechnology and Food Science 5 (1) 40-44.

      [24] Pahujani S, Kanwar SS, Chauhan G & Gupta R (2008) Glutaraldehyde activation of polymer Nylon-6 for lipase immobilization: enzyme characteristics and stability. Bioresource Technology 99 (7), 2566–2570.

      [25] Shabbiri K & Adnan A (2011) Bio-statistically optimized production of lipases by Brevibacterium linens DSM 20158. World Applied Science Journal 13, 1059–1066.

      [26] Tanyildizi MS, Ozer D & Elibol M (2005) Optimization of α amylase production by Bacillus sp. using response surface methodology, Process Biochemistry 40, 2291-2296.

      [27] West TP & Strohfus BRH (1996) Polysaccharide production by sponge –immobilized cells of the fungus Aureobasidium pullulan. Letters in Applied Microbiology 22, 162-164.

      [28] Yagiz F, Kazan D & Akin N (2007) Biodiesel production from waste oils by using lipase immobilized on hydrocalcite and zeolites. Chemical Engineering Journal 134, 262–267.




Article ID: 6008
DOI: 10.14419/ijbr.v4i1.6008

Copyright © 2012-2015 Science Publishing Corporation Inc. All rights reserved.