Production of ethanol and clarification of apple juice by pectinase enzyme produced from Aspergillus terreus NCFT 4269.10

  • Authors

    • Bijay Sethi MITS School of Biotechnology
    • Amrita Satpathy MITS School of Biotechnology
    • Subodh Tripathy MITS School of Biotechnology
    • Sidarth Parida MITS School of Biotechnology
    • Sameer Kumar Singdevsachan
    • Bikash Behera North Orissa University
    2016-05-22
    https://doi.org/10.14419/ijbr.v4i1.6134
  • Aspergillus terreus, Banana Peels, Fermentation, Wheat Bran.
  • Abstract

    Aspergillus terreus NCFT 4269.10 was evaluated by liquid static surface fermentation (LSSF), shaking fermentation (ShF) and solid state fermentation (SSF) for the production of pectinase. Among various substrates tested, banana peels supported maximum production of pectinase i.e. 1000 ± 141.42 U/ml. The biomass of A. terreus was maximum with wheat bran (0.55±0.07g/50ml). Pectinase produced by A. terreus displayed higher specific activity when wheat bran was used as the sole source of carbon and energy. After successful fermentation, crude enzyme was purified to electrophoretic homogeneity with a specific activity of 1846.50 U/mg from an initial specific activity of 1282.05 U/mg. The cell free-dialyzed-enzyme containing 107100 U was purified to 1.44 fold with an overall enzyme yield of 35.70%.Immobilization study revealed that the production of pectinase was increased up to third cycle and decreased thereafter when further pectinase production was carried out by immobilized spores of A. terreus.

  • References

    1. [1] Adeleke AJ, Odunfa SA, OlanbiwonninuA & Owoseni MC (2012) Production of cellulase and pectinase from orange peels by fungi. Nature and Science 10(5), 107–112.

      [2] Alkorta I, Gabirsu C, Lhama MJ & Serra JL (1998) Industrial applications of pectic enzymes: a review. Process Biochemistry 33, 21-8.http://dx.doi.org/10.1016/S0032-9592(97)00046-0.

      [3] Alvarez S, Alvarez R, Riera FA & Coca J (1998) Influence of depectinization on apple juice ultrafiltration. Colloids and Surfaces A: Physicochemical and Engineering Aspects 138, 377-82.http://dx.doi.org/10.1016/S0927-7757(98)00235-0.

      [4] Amerine MA & Ough CS (1984). Wine and must analysis. New York (USA), Wiley.

      [5] Beg QK,Kapoor M, Tiwari RP & Hoondal GS (2001) Bleach-boosting of eucalyptus kraft pulp using combination of xylanase and pectinase from Streptomyces sp. QG-11-3. Research Bulletin of Panjab University of Science 51, 71-78.

      [6] Bhat MK (2006) Cellulases and related enzymes in biotechnology. Biotechnology Advances, 18, 355-83.http://dx.doi.org/10.1016/S0734-9750(00)00041-0.

      [7] Celestino SMC, Freitas SM, Medrano FJ, De Sousa MV & Ferreira-Filho EX (2006) Purification and characterization of a novel pectinase from Acrophialophoranainiana. Journal of Biotechnology 123, 33-42.http://dx.doi.org/10.1016/j.jbiotec.2005.10.024.

      [8] Contreas-Esquivel JC & Voget CE (2004) Purification and partial characterization of an acid polygalacturonase from Aspergillus kawakii. Journal of Biotechnology 110, 21-8.http://dx.doi.org/10.1016/j.jbiotec.2004.01.010.

      [9] de Vries RP, Jansen J, Aguilar G, Parenicova L, Joosten V, WulfertF, BenenJA &Visser J (2002) Expression profiling of pectinolytic genes from Aspergillusniger. FEBS Letter 530, 41–47.http://dx.doi.org/10.1016/S0014-5793(02)03391-4.

      [10] Delgado LAT, Huitron BC & Aguilar G (1992) Pectin lyase from Aspergillus sp. CH-Y-1043. AppliedMicrobiology andBiotechnology 39, 515-519.http://dx.doi.org/10.1007/BF00205043.

      [11] Dhillon SS, Gill RK, Gill SS & Singh M (2004) Studies on the utilization of citrus peel for pectinase production using Aspergillus niger. International Journal of Environment 61, 199 – 210.

      [12] El-Katatny MH, Hetta AH, Shaban GM & El-Komy HM (2003) Enzyme Production by Alginate Encapsulated Trichodermaspp. Food Technology & Biotechnology 41 (3), 219–225.

      [13] Fawole OB & Odunfa SA (2003) some factors affecting production of peptic enzymes by Aspergillus niger. International Biodeterioration & Biodegradation 52, 223-227.http://dx.doi.org/10.1016/S0964-8305(03)00094-5.

      [14] Gummadi SN & Panda T (2003) Purification and biochemical properties of microbial pectinases – a review. Process Biochemistry 38, 987-96.http://dx.doi.org/10.1016/S0032-9592(02)00203-0.

      [15] Iftikhar T, Niaz M, Zia MA & Haq IU (2010) Production of extracellular lipases by Rhizopus oligosporusin a stirred fermentor. Brazilian Journal of Microbiology 41, 1124–1132. http://dx.doi.org/10.1590/S1517-83822010000400034.

      [16] Jacob N, Poorna AC & Prema P (2008) Purification and partial characterization of polygalacturonase from Streptomyces lydicus. Bioresource Technology 99, 6697-701.http://dx.doi.org/10.1016/j.biortech.2007.10.002.

      [17] Jana A, Maity C, Halder SK, Das A, Pati BR, Mondal KC & Das Mohapatra PK (2013) Structural characterization of thermostable, solvent tolerant, cytosafe tannase from Bacillus subtilis PAB2. Biochemical Engineering Journal 77, 161–170.http://dx.doi.org/10.1016/j.bej.2013.06.002.

      [18] Kashyap DR, Vohra PK, Chopra S & Tewari R (2001) Applications of pectinase in the commercial sector: a review. Bioresource Technology 77, 215–227.http://dx.doi.org/10.1016/S0960-8524(00)00118-8.

      [19] Khairnar Y, Vamsi KK, Boraste A, Gupta N, Trivedi S, Patil P, Gupta G, Gupta M, Jhadav A, Mujapara A, Joshi B & Mishra D (2009) Study of pectinase production in submerged fermentation using different strains of Aspergillus niger. International Journal of Microbiological Research 1, 13–17.http://dx.doi.org/10.9735/0975-5276.1.2.13-17.

      [20] KropJJP & PilnikW (1974) Effect of pectic acid and bivalent cations on cloud loss of citrus juice. Lebensmittel-Wissenschaft und-Technologie 7, 62–73.

      [21] Kumar A & Duhan JS (2011) Production and characterization of amylase enzyme isolated from Aspergillusniger MTCC–104 employing solid state fermentation. International Journal of Pharam& Bio Science 3(2), 250–258.

      [22] Miranda OA, Salgueiro AA, Pimentel MCB, Lima Filho JL, Melo, EHM & Dur´an N (1999) Lipase production by a Brazilian strain of Penicilliumcitrinumusing an industrial residue. Bioresource Technology 69, 145–147. http://dx.doi.org/10.1016/S0960-8524(98)00166-7.

      [23] Mosier N & Wyman C (2005) Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresource Technology 96, 673–686.http://dx.doi.org/10.1016/j.biortech.2004.06.025.

      [24] Naidu GSN & Panda T (1998) Production of pectolytic enzymes: a review. Bioprocess Engineering 19, 355-361. http://dx.doi.org/10.1007/PL00009023.

      [25] Namasivayam E, Ravindar JD, Mariappan K, Akhil J, Mukesh K & Jayaraj RL (2011) Production of extracellular pectinase by Bacillus cereus isolated from market solid waste. Journal of Bioanalysis& Biomedicine 3, 070-075.http://dx.doi.org/10.4172/1948-593X.1000046.

      [26] Pedrolli DB (2008) Caracterizaçãofisico-química de pectinases extracelularespurificadas de Aspergillus giganteus. M.Sc. Thesis, São Paulo State University, Rio Claro, São Paulo, Brazil.

      [27] Reid I & Richard M (2004) Purified pectinase lowers cationic demand inperoxide-bleached mechanical pulp. Enzyme & Microbial Technology 34, 499–504.http://dx.doi.org/10.1016/j.enzmictec.2003.12.005.

      [28] Ribeiro DS, Henrique SMB, Oliveira LS, Macedo GA & Fleuri LF (2010) Enzymes in juice processing: a review. International Journal of Food Science and Technology 45, 635-641.http://dx.doi.org/10.1111/j.1365-2621.2010.02177.x.

      [29] Rombouts FM & Pilnik W (1980) Pectic enzymes. In Rose AH, Ed. Microbial Enzymes and Bioconversions Academic Press, London, 5, pp. 227-72

      [30] Salazar L & Jayasinghe U (1999) Fundamentals of purification of plant viruses. In Techniques in plant virology CIP. Training Manual JO Virus Purification Int Potato Centre, Peru, pp. 1–10.

      [31] Semenova MV, Sinitsyna OA, Morozova VV, Fedorova EA, Gusakov AV, Okunev ON, Sokolova LM, Koshelev AV, BubnovaIu P & Sinitsyn AP (2006) Use of a preparation from fungal pectin lyase in the food industry. Applied Biochemistry and Microbiology 42, 598-602.http://dx.doi.org/10.1134/S000368380606010X.

      [32] Sethi BK, Nanda PK & Sahoo SL (2016) Enhanced production of pectinase by Aspergillus terreus NCFT 4269.10 using banana peels as substrate. 3 Biotech 6, 36-51.http://dx.doi.org/10.1007/s13205-015-0353-y.

      [33] Sethi BK, Panda B & Sahoo SL (2015) Biosynthesis of industrially relevant extracellular pectinase from Aspergillus terreus NCFT 4269.10 using orange peels as substrate. Asian Journal of Biotechnology 7, 1322.http://dx.doi.org/10.3923/ajbkr.2015.13.22.

      [34] Sethi BK, Rout JR, Das R, Nanda PK &Sahoo SL (2013) Lipase production by Aspergillus terreus using mustard seed oil cake as a carbon source. Annals of Microbiology 63, 241–252.http://dx.doi.org/10.1007/s13213-012-0467-y.

      [35] Sharma DC & Satyanarayana T (2005) A marked enhancement in the production of a highly alkaline and thermostable pectinase by Bacillus pumilus dcsr1 in submerged fermentation by using statistical methods. Bioresource Technology 97, 727–733.http://dx.doi.org/10.1016/j.biortech.2005.04.012.

      [36] Singh SA, Ramakrishna M & Rao AGA (1999) Optimization of downstream processing parameters for the recovery of pectinase from the fermented broth of Aspergillus carbonarious. Process Biochemistry 35, 411–417.http://dx.doi.org/10.1016/S0032-9592(99)00089-8.

      [37] Soares MMCN, da Silva R, Carmona EC & Gomes E (2001) Pectinolytic enzyme production by Bacillus species and their potential application on juice extraction. Journal of Microbiology & Biotechnology 17, 79-82.http://dx.doi.org/10.1023/A:1016667930174.

      [38] Torimiro N & Okonji RE (2013) a comparative study of pectinolytic enzyme production by Bacillus species. African Journal of Biotechnology 12(46), 6498-6503.http://dx.doi.org/10.5897/AJB2013.12421.

      [39] Vibha B & Neelam G (2010) Exploitation of microorganisms for screening of pectinase from environment. 8th International Conference. Globelics.

      [40] Viikari L, Tenakanen M & Suurnakki A (2001) Biotechnology in the pulp and paper industry. In Rehm HJ, editor, Biotechnology, VCH-Wiley, pp. 523-546.http://dx.doi.org/10.1002/9783527620937.ch18.

      [41] Whitaker JR (1990) Microbial pectinolytic enzymes. In Fogarty WM, Kelly CT, editors. Microbial enzymes and biotechnology 2nd ed. Elsevier Science Ltd, London, pp. 133-176.http://dx.doi.org/10.1007/978-94-009-0765-2_4.

      [42] Will F, Bauckhage K & Dietrich H (2000) Apple pomace liquefaction with pectinases and cellulases: analytical data of the corresponding juices. Europian Food Research Technology 211, 291-297.http://dx.doi.org/10.1007/s002170000171.

      [43] Yamaski M, Yasui T & Arima K (1964) Pectic enzymes in the clarification of apple juices. Part I Study on the clarification reaction in a simplified model. Agricultural & Biological Chemistry 28, 779-787.

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  • How to Cite

    Sethi, B., Satpathy, A., Tripathy, S., Parida, S., Singdevsachan, S. K., & Behera, B. (2016). Production of ethanol and clarification of apple juice by pectinase enzyme produced from Aspergillus terreus NCFT 4269.10. International Journal of Biological Research, 4(1), 67-73. https://doi.org/10.14419/ijbr.v4i1.6134

    Received date: 2016-04-18

    Accepted date: 2016-05-10

    Published date: 2016-05-22