Production process and characterization of extra cellular li-pids from bacterial strain from oil industries waste

  • Authors

    • Vadivel Balamurugan PG and Research Department of Biotechnology, Sri Vinayaga College of Arts and Science,Ulundurpet-606 107, Tamil Nadu, India.
    • Veluchamy Balakrishnan PG and Research Department of Botany, Arignar Anna Government Arts College, Namakkal- 637 002, Tamil Nadu, India.
    • Arjunan Sundaresan PG and Research Department of Biotechnology, Sri Vinayaga College of Arts and Science,Ulundurpet-606 107, Tamil Nadu, India.
    • Kodhandapani Vasanthi PG and Research Department of Biotechnology, Sri Vinayaga College of Arts and Science,Ulundurpet-606 107, Tamil Nadu, India.
    • Selvaraj Venkatesan PG and Research Department of Biotechnology, Sri Vinayaga College of Arts and Science,Ulundurpet-606 107, Tamil Nadu, India.
    2016-05-24
    https://doi.org/10.14419/ijbr.v4i1.6161
  • Bacillus species, Bacterial strain, Extra cellular lipids, lipase enzyme, Oil industries waste.

  • Abstract

    The Bacillus sp was isolated from oil industry waste. The isolated strain was screened for the production of lipase enzyme. The production was done by shake flask fermentation. After downstream processing, the partial purification was done my ammonium sulphate precipitation & dialysis and the assay was done by photometric method. The various factors affecting production of extra cellular lipase activity was assayed which include pH, different substrate, temperature and additives. Besides, production was made using different carbon source and crude medium. Result showed that pH 6 and 37°C is an optimum environmental parameter for the growth of the isolate. In addition, the sucrose was found to be better carbon source.

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  • How to Cite

    Balamurugan, V., Balakrishnan, V., Sundaresan, A., Vasanthi, K., & Venkatesan, S. (2016). Production process and characterization of extra cellular li-pids from bacterial strain from oil industries waste. International Journal of Biological Research, 4(1), 74-77. https://doi.org/10.14419/ijbr.v4i1.6161

    Received date: 2016-04-25

    Accepted date: 2016-05-18

    Published date: 2016-05-24