Grazing intensity contributes to cyanogenic toxicity in savannah grasses in Baringo county

 
 
 
  • Abstract
  • Keywords
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  • Abstract


    The potential role of anti-herbivory mechanisms used by plants and their synergistic responses to grazing and interactive effects on herbivores are poorly understood. The aim of this study was to quantify the influence of grazing intensity on cyanogenic glycosides in Lake Bogoria, Baringo County Kenya. Field experiments were carried out in ten 50×10m enclosures. Grazing intensity was varied using simulated grazing method where two grazing treatments used; heavy grazing and light grazing. Grasses were categorized into two age classes; young and old. Cyanigenic glycocides (CNglc) were tested using impregnated picrate paper and their concentration determined by hydrolysis and trapping in 1M NaOH. Our findings showed that five of 16 sampled species produce cyanogenic glycosides; Cynodon dactylon, Cynodon plectostachyus, Digitaria scalarum, Sporobolus spicatus and Cyperus laevigatus. There was an inverse relation between Cyanide concentration and age of the plants. Young cuttings yield more Hydrogen Cyanide than older cuttings of the same grasses.Grazing intensity had a significant effect on the concentration of cyanogenic content in some grass species; C.dactylon (P=0.024) and S. laevigatus (P=0.003). The findings imply that grazing regime of managed pastures should consider the age of forage while allowing utilization of pastures preferably grazed on mature pastures with low levels of cyanogenic glycosides.


  • Keywords


    Cyanogenic; Glycosides Grasses; Grazing and Intensity.

  • References


      [1] Agrawal AA (2011). New synthesis—trade-offs in chemical ecology. Journal of chemical ecology, 37(3), 230-231. http://dx.doi.org/10.1007/s10886-011-9930-7.

      [2] Ballhorn DJ. (2011) Constraints of simultaneous resistance to a fungal pathogen and an insect herbivore in Lima bean (Phaseolus lunatus L.) Journal of Chemical Ecology 37:141–44 http://dx.doi.org/10.1007/s10886-010-9905-0.

      [3] Ballhorn DJ, Kautz S & Lieberei R (2010) Comparing responses of generalist and specialist herbivores to various cyanogenic plant features. Entomologia Experimentalis et Applicata, 134(3), 245-259. http://dx.doi.org/10.1111/j.1570-7458.2009.00961.x.

      [4] Ballhorn DJ, Kautz S, & Schadler M (2013) Induced plant defense via volatile production is dependent on rhizobial symbiosis. Oecologia 172:833–46 http://dx.doi.org/10.1007/s00442-012-2539-x.

      [5] Ballhorn DJ, Schiwy S, Jensen M, Heil M (2008) Quantitative variability of direct chemical defense in primary and secondary leaves of lima bean (Phaseolus lunatus) and consequences for a natural herbivore. Journal Chemical Ecology 34:1298–30 http://dx.doi.org/10.1007/s10886-008-9540-1.

      [6] Busk, PK, Møller BL (2002) Dhurrin synthesis in sorghum is regulated at the transcriptional level and induced by nitrogen fertilization in older plants. Plant Physiology 129:1222–31 http://dx.doi.org/10.1104/pp.000687.

      [7] Ebbs (2004) Biological degradation of cyanide compounds. Journal of Biotechnology, 15, 231-236. http://dx.doi.org/10.1016/j.copbio.2004.03.006.

      [8] Ernesto M, Cardoso AP, Nicala D, Mirione E, Massaza F, Cliff J, Haque MR & Bradbury JH (2002) Persistent konzo and cyanogens toxicity from cassava in northern Mozambique. Acta Tropica 82, 357-362. http://dx.doi.org/10.1016/S0001-706X(02)00042-6.

      [9] Forslund K, Morant M, Jørgensen B, Olsen, CE, Asamizu E (2004) Biosynthesis of the nitrile glucosides rhodiocyanoside A and D and the cyanogenic glucosides lotaustralin and linamarin in Lotus japonicus. Plant Physiology. 135:71–84 http://dx.doi.org/10.1104/pp.103.038059.

      [10] Ganjewala, D, Kumar S, Devi SA, & Ambika K (2010) Advances in cyanogenic glycosides biosynthesis and analyses in plants: A review. Acta Biologica Szegediensis, 54(1), 1-14.

      [11] Gleadow RM & Woodrow IE (2000) Temporal and spatial variation in cyanogenic glycosides in Eucalyptus cladocalyx. Tree Physiology 20:591–98 http://dx.doi.org/10.1093/treephys/20.9.591.

      [12] Gleadow RM, Woodrow IE (2002) Constraints on effectiveness of cyanogenic glycosides in herbivore defense. Journal of Chemical Ecology 28:1301–13 http://dx.doi.org/10.1023/A:1016298100201.

      [13] Goodger JD, Gleadow RM & Woodrow IE (2006) Growth cost and ontogenetic expression patterns of defense in cyanogenic Eucalyptus spp. Trees science 20:757–65 http://dx.doi.org/10.1007/s00468-006-0090-2.

      [14] Jørgensen K, Morant AV, Morant M, Jensen NB, Olsen CE (2011). Biosynthesis of the cyanogenic glucosides linamarin and lotaustralin in cassava: isolation, biochemical characterization, and expression pattern of CYP71E7, the oxime-metabolizing cytochrome P450 enzyme. Plant Physiology 155:282–92 http://dx.doi.org/10.1104/pp.110.164053.

      [15] Kadow D, Voß K, Selmar D, Lieberei R (2012) the cyanogenic syndrome in rubber tree Hevea brasiliensis: tissue-damage-dependent activation of linamarase and hydroxynitrile lyase accelerates hydrogen cyanide release. Annual Botany Reviews 109:1253–62 http://dx.doi.org/10.1093/aob/mcs057.

      [16] Kempel A Brandl R & Schadler M (2009) Symbiotic soil microorganisms as players in aboveground plant-herbivore interactions: the role of rhizobia. Oikos 118:634–40 http://dx.doi.org/10.1111/j.1600-0706.2009.17418.x.

      [17] Lee J, Zhang G, Wood E, Castillo CR & Mitchell AE (2013) Quantification of amygdalin in non-bitter, Semi-bitter, and bitter almonds (Prunus dulcis) by UHPLC-(ESI) QQMS/MS. Journal of Agriculture and Food Chemical 61:7754–59 http://dx.doi.org/10.1021/jf402295u.

      [18] McBee GG, Miller FR (1980) Hydrocyanid acid potential in several sorghum breeding lines as affected by nitrogen fertilization and variable harvests. Crop Science. 20:232–35 http://dx.doi.org/10.2135/cropsci1980.0011183X002000020020x.

      [19] Miller RE, Gleadow RM, Cavagnaro, TR (2014) Age versus stage: Does ontogeny modify the effect of phosphorus and arbuscular mycorrhizas on above- and below-ground defense in forage sorghum? Plant Cell Environment 37:929–42 http://dx.doi.org/10.1111/pce.12209.

      [20] Mithöfer A & Boland W (2012) Plant defense against herbivores: chemical aspects. Annual review of plant biology, 63, 431-450. http://dx.doi.org/10.1146/annurev-arplant-042110-103854.

      [21] Møller BL (2010) Functional diversifications of cyanogenic glucosides. Current opinion in plant biology, 13(3), 337-346 http://dx.doi.org/10.1016/j.pbi.2010.01.009.

      [22] Neilson EH, Goodger JQ, Woodrow IE & Møller BL (2013) Plant chemical defense: at what cost? Trends Plant Sci. 18:250–58 http://dx.doi.org/10.1016/j.tplants.2013.01.001.

      [23] Nishida R (2002) Sequestration of defensive substances from plants by Lepidoptera. Annual Reviews. Entomology 47:57–92 http://dx.doi.org/10.1146/annurev.ento.47.091201.145121.

      [24] O’Donnell NH, Møller BL, Neale AD, Hamill JD, Blomstedt CK, Gleadow RM (2013) Effects of PEG-induced osmotic stress on growth and dhurrin levels of forage sorghum. Journal of Plant Physiology 73:83–92 http://dx.doi.org/10.1016/j.plaphy.2013.09.001.

      [25] Pentzold S, Zagrobelny M, Roelsgaard PS, Møller BL, & Bak S (2014) The multiple strategies of an insect herbivore to overcome plant cyanogenic glucoside defence. Journal of Ecology, 9(3), 913-37. http://dx.doi.org/10.1371/journal.pone.0091337.

      [26] Ramirez CA, & Barry TN (2005) Alternative temperate forages are containing secondary compounds for improving sustainable productivity in grazing ruminants. Animal Feed Science and Technology, 120(3), 179-201. http://dx.doi.org/10.1016/j.anifeedsci.2005.01.015.

      [27] Schappert PJ, Shore JS (1999) Cyanogenesis, herbivory and plant defense in Turnera ulmifolia on Jamaica. Journal of Ecoscience 6:511–20

      [28] Schappert PJ & Shore JS (2000) Cyanogenesis in Turnera ulmifolia L. (Turneraceae): Developmental expression, heritability and cost of cyanogenesis. Evolutionary Ecological Resources 2:337–52

      [29] Selmar D & Kleinwachter M (2013) Stress enhances the synthesis of secondary plant products: the impact of stress-related over-reduction on the accumulation of natural products. Plant Cell Physiology 54:817–26 http://dx.doi.org/10.1093/pcp/pct054.

      [30] Sirikantaramas S, Yamazaki, M, & Saito, K (2008) Mechanisms of resistance to self-produced toxic secondary metabolites in plants. Phytochemistry Reviews, 7(3), 467-477. http://dx.doi.org/10.1007/s11101-007-9080-2.

      [31] Stochmal A, Oleszek W (1997) Changes of cyanogenic glucosides in white clover (Trifolium repens L.) during the growing season. Journal of Agriculture 45:4333–36

      [32] Takos AM, Lai D, Mikkelsen L, Maher AH & Shelton, D, (2010). Genetic screening identifies cyanogenesis-deficient mutants of Lotus japonicus and reveals enzymatic specificity in hydroxynitrile glucoside metabolism Plant Cell 22:1605–19 http://dx.doi.org/10.1105/tpc.109.073502.

      [33] Ubalua AO (2010) Cyanogenic Glycosides and the fate of cyanide in Australian Journal of Crop Science, 4(4), 223-237 University of Chicago Press

      [34] Vandegeer R, Miller RE, Bain M, Gleadow RM & Cavagnaro TR (2013) Drought adversely affects tuber development and nutritional quality of the staple crop cassava (Manihot esculenta Crantz). Plant Biology 40:195–200 http://dx.doi.org/10.1071/fp12179.

      [35] Webber BL & Woodrow IE (2009) Chemical and physical plant defense across multiple ontogenetic stages in a tropical rain forest under storey tree Journal of Ecology 97:761–71 http://dx.doi.org/10.1111/j.1365-2745.2009.01512.x.

      [36] Wheeler JL, Mulcahy C, Walcott JJ, & Rapp GG (1990) Factors affecting the hydrogen cyanide potential of forage sorghum. Aust. Journal of Agricultural Resources 41:1093–100 http://dx.doi.org/10.1071/AR9901093.

      [37] Woodrow IE, Slocum DJ & Gleadow RM (2002) Influence of water stress on cyanogenic capacity in Eucalyptus cladocalyx. Functional Plant Biology, 29(1), 103-110 http://dx.doi.org/10.1071/PP01116.

      [38] Zagrobelny M, Bak S, Rasmussen AV, Jorgensen B, Naumann CM & Møller BL (2004) Cyanogenic glucosides and plant–insect interactions. Phytochemistry 65:293–306 http://dx.doi.org/10.1016/j.phytochem.2003.10.016.

      [39] Zagrobelny M, Bak S, Olsen CE, Møller BL (2007) Intimate roles for cyanogenic glucosides in the life cycle of Zygaena filipendulae (Lepidoptera, Zygaenidae). Journal of Molecular Biology 37:1189–97 http://dx.doi.org/10.1016/j.ibmb.2007.07.008.

      [40] Zagrobelny M & Møller BL (2011) Cyanogenic glucosides in the biological warfare between plants and insects: the Burnet moth-birdsfoot trefoil model system. Phytochemistry 72:1585–92 http://dx.doi.org/10.1016/j.phytochem.2011.02.023.

      [41] Zagrobelny M, Bak S & Møller BL (2008) Cyanogenesis in plants and arthropods. Phytochemistry, 69(7), 1457-1468. http://dx.doi.org/10.1016/j.phytochem.2008.02.019.

      [42] Zhu-Salzman K, Salzman RA, Ahn JE, Koiwa H (2004) Transcriptional regulation of sorghum defense determinants against a phloem-feeding aphid. Plant Physiology. 134:420–31. http://dx.doi.org/10.1104/pp.103.028324.


 

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Article ID: 6240
 
DOI: 10.14419/ijbr.v4i2.6240




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