Phytochemical screening, FT-IR analysis and antimicrobial activity of Wattakaka volubilis

  • Authors

    • Venkatesan Selvaraj PG and Research Department of Biotechnology, Sri Vinayaga College of Arts and Science, Ulundurpet, 606 107, Tamil Nadu, India
    • Anithakumari M. PG and Research Department of Biotechnology, Sri Vinayaga College of Arts and Science, Ulundurpet, 606 107, Tamil Nadu, India
    • Balamurugan V. PG and Research Department of Biotechnology, Sri Vinayaga College of Arts and Science, Ulundurpet, 606 107, Tamil Nadu, India
    • Sundaresan Arjunan PG and Research Department of Biotechnology, Sri Vinayaga College of Arts and Science, Ulundurpet, 606 107, Tamil Nadu, India
    • Susindren Perumal PG and Research Department of Biotechnology, Sri Vinayaga College of Arts and Science, Ulundurpet, 606 107, Tamil Nadu, India
    • Vasanthi K. PG and Research Department of Biotechnology, Sri Vinayaga College of Arts and Science, Ulundurpet, 606 107, Tamil Nadu, India
    2016-08-23
    https://doi.org/10.14419/ijbr.v4i2.6457
  • Wattakaka volubilis, Phenols, Ethanol, DMSO, Bacillus Pumilus, Bacteria, Tannin Etc.

  • The present study was evaluated the phytochemical analysis and antibacterial activity of Wattakaka volubilis leaf extract. The Wattakaka volubilis is medicinal plant used in the treatment of various diseases (Wound, diabetics, rheumatic and diarrhea). The ethanol, methanol, petroleum ether and DMSO extract from the leaf of Wattakaka volubilis were screened on their phytochemicals analysis. Among thesePhytochemicals were alkaloids, anthroquione, caumarin, flavanoids, phenols, saponins, steroids, tannins and terpenoids for present in the following extracts ethanol, methanol. The petroleum ether and DMSO only present in several phytochemicals like alkaloid, anthroquinone, terpenoids and tannins. Wattakaka volubilis against the antibacterial like E. coli, B. pumilus, Enterobacter aerogens, and Pseudomonas aeruginosa. The zone of inhibition of Wattkaka voulubilis leaf extract against the bacteria was maximum inhibition Pseudomonas aeruginosa, Enterobacter aerogens, Bacillus pumilis. The least zone of inhibition was recorded against E.coli. The characterization of the plant extract and the functional groups was analyzed in Fourier Transform Infrared Spectroscopy (FTIR). The absorbance bands analysis were observed in the region of 4000-400cm-1 are 23 compounds derived. The compounds represented the ranges from 3905.21 to 436.41 peaks were shown. This study which is the primary report on the phytochemicals analysis and antibacterial properties of Wattakaka volubilis supports its traditional uses in the treatment of infectious and non- infectious diseases.

  • References

    1. [1] Agarwal, V.S., (1986). Economic plants of India, KailashPrakashan, Calcutta: 116.

      [2] Arts, I.C. andHollmn, P.C., (2005).Polyhenols are disease risk in epidemiological studies. American Journal of Clinical Nutrition; 81:317-325.

      [3] Balakumar, S., Rajan, S., Thirunalasundari, T., and Jeeva, S., (2011).Antifungal activity of Leucasaspera(L.)Correa(Rutaceae) leaf extract on dermatophytes. Asian. Pac. J.Trop Biomed; 309-312.http://dx.doi.org/10.1016/S2221-1691(11)60049-X.

      [4] Beyer, R.E., (1994). The role of ascorbate in antioxidant protection of biomembranes: interaction with vit-E and coenzyme. Q. J. Bioen. Biomemb; 24: 349-358.http://dx.doi.org/10.1007/BF00762775.

      [5] Biju, P.G,,Gayatri, D.V., Lija, Y., and Abraham, A., (2007). Protection against Selenite Cataract

      [6] Hulin, V., Mathot, A.G., Mafart, P. andDufosse L., (1998): Less proprieties anti-microbiennesdeshuilesessentialness composes drones. Sci.Aliments; 18: 563-582.

      [7] Fatimah, Z.I., Zaiton, Z., Zamaludin, M., Gaptor, M.T., Nafeeza, M.L., Khairul, O., (1998).Effect on estrogen and palm vitamin F on malonaldehyde levels toward the development of arteriosclerosis in the New Zealand white rabbit. In: Packer L, Ong SH, editors. Biological Oxidants and antioxidants: Molecular Mechanism and Health Effects. Champaign IL: AOCS Press; 22.

      [8] Molisha, B., Bikash, M.N., Partha, P., Ashoke, G. and Bannerjee, S. and Kanti, H.P., (2009). In vitro anti- leishmanial and anti-tumour activities of a pentacyclictriterpenoid compound isolated from the fruits of DregeavolubilisBenthAsclepiadaceae. Trop. J. Pharm. Res: 8(2): 127- 131.

      [9] Raghavendra, M.P., Satish, S. and Raveesha, K.A., (2006). Phytochemical analysis and antibacterial activity of Oxalis corniculata: A known medicinal plant. My. Science; 1: 72-78.

      [10] Raghavendra, M.P., Satish, S. and Raveesha, K.A., (2006). Phytochemical analysis and antibacterial activity of Oxalis corniculata: A known medicinal plant. My. Science; 1: 72-78.

      [11] Thangavelu, T. Gnaphaliumpolycaulon, ShanmugapriyaKaminidevi, JinuUdayabhanu, SenthilMuruganThangave., (2015). Antimicrobial activity of methanolic extracts of indigenous traditional Indian folk Medicinal Plant. International Journal of Green Pharmacy: 1-6.

      [12] Wangensteen, H.; Samuelsen, A.B. and Malterud, K.E., (2004) Antioxidant activity in extracts from coriander.Food Chem. 88, 293-297.http://dx.doi.org/10.1016/j.foodchem.2004.01.047.

      [13] Yeh, G.Y., Eisenberg, D.M., Kaptchuk, T.J., Phillips, R.S., 2003. Systematic Review of Herbs and Dietary Supplements for Glycemic Control in Diabetes. Diabetes Care 26, 1277 – 1294.http://dx.doi.org/10.2337/diacare.26.4.1277.

      [14] Guptha, A.K., Neeraj, T. and Sharma, M., (2005).Quality standards of Indian Medicinal Plants.Vol 3, ICMR,New Delhi; 9-19.

      [15] Harbone, J.B., (1993). Phytochemistry,Acad.Press London, 21: 2785.

      [16] Kirtukar, K.R and Basu, B.D., (2003). Indian medicinal plants, Bishen Singh Mahendrapal Singh, Dehradum; 3: 1635.

      [17] Matthew, K.M., (1983). The Flora of Tamil Nadu Carnatic.InTheRapinatHerbarium.St Joseph's College, Tiruchirapalli, India.

      [18] Patil, R. H., Chaudhary, B., and Settipalli, S.,(2009). Antifungal and Antiaflatoxigenic activity of LeucasasperaLinn.PharmacognosyJournol.1 (4): 345-351.

      [19] Pitre, S., and Srivastava S.K., (1987). Pharmacological, microbiological and phytochemical studies on the root of Leucasaspera.Fitoterapia 58: 194 -198.

      [20] Rana, B. K., Singh, U. P. and Taneja, V., (1997). Anti-fungal activity and kinectics of inhibition by essential oil isolated from leaves of Leucasaspera.JEthanopharmacol; 57:29-34 http://dx.doi.org/10.1016/S0378-8741(97)00044-5.

      [21] Ravi, K., Rajasekaran, S., Subramanian, S., 2005.Antihyperlipidemic effect of Eugenia jambolana seed kernel on streptozotocin induced diabetes in rats. Food and Chemical Toxicology 43, 1433 – 1439.http://dx.doi.org/10.1016/j.fct.2005.04.004.

      [22] Sastry, V.M.S., and Rao, G.R.K.,(1994). Antibacterial substance from marine algae: successive extraction using benzene, chloroform and methanol. Bot. Mar.37: 357-360. http://dx.doi.org/10.1515/botm.1994.37.4.357.

      [23] Subbiah, V., (2005). Natural product based apoptosis inducers. United States patent application 0084547.

      [24] Raghavendra, M.P., Satish, S. and Raveesha, K.A., (2006). Phytochemical analysis and antibacterial activity of Oxalis corniculata: A known medicinal plant. My. Science; 1: 72-78.

      [25] Vlachos, V., Critchley, A. T., and Von Holy, A., (1997).Antimicrobial activity of extracts from selected Southern African marine macroalgae.SouthAfr J.Sci.93:328-332.

      [26] Yoshimura, S., Narita, H., Hayashi, K. and Mitsuhashi H., (1983).Studies on the constituents of asclepiadaceae plants. LVI. Isolation of new antitumor-active glycosides from Dregeavolubilis(L.) BENTH.Chem Pharm Bull (Tokyo); 31:3971-83.

      [27] Perez, C., Paul, M. and Bazerque, P., (1990).An antibiotic assay by the agar well diffusion method.ActaBiol Med Exp; 15: 113-115.

      [28] Saifuddin, N., C.W. Wong and A.A. NurYasumira, 2009. Rapid biosynthesis of silver nanoparticles using culture supernatant of bacteria with microwave irradiation. E-J. Chem., 6: 61-70.http://dx.doi.org/10.1155/2009/734264.

      [29] Lustigman, B., and Brown, C., (1991). Antibiotic production by marine algae isolated from the New York/New Jersey Coast BulletinEnvironmental Contamination and Toxicology. 46:329- 335.http://dx.doi.org/10.1007/BF01688928.

      [30] Essawi, T. and Srour, M., (2000).Screening of some palestian medicinal plants for antibacterial activity.J. Ethanopharmacol; 70: 343-349.http://dx.doi.org/10.1016/S0378-8741(99)00187-7.

      [31] Divya, T.S., (2009). Anti-inflammatory, analgesic and anti-lipid peroxidative properties of Wattakakavolubilis (L.f.) Stapf.Nat. Prod. Radi; 8(2):137-141.

      [32] Vijayal, T., Devamma, N.M. and Nirmala, C., (2008).Antibacterial and antifungal activity of Marsdeniavolubilis in in vitroconditions.International seminar on medicinal plants.

      [33] Sahu, N., Panda, N., Mandal, N.B., Banerjee, S., Koike, K. and Nikaido, T., (2002). Polyoxypregnane glycosides from the flowers of Dregeavolubilis.Phytochem; 61:383-8. http://dx.doi.org/10.1016/S0031-9422(02)00260-1.

      [34] Pandikumar P, Ayyanar M and Ignacimuthu S, 2007. Medicinal plants used by Malasar tribes of Coimbatore district, Tamil Nadu. Indian Journal of Traditional Knowledge, 6, 579-582.

      [35] Silija VP, SamithaVarma K and Mohanan KV, 2008. Ethnomedicinal plant knowledge of the Mullukuruma tribe of Wayanad district, Kerala,Indian Journal of Traditional Knowledge,7, 604-612.

      [36] Ayyanar M, SankaraSivaraman K and Ignacimuthu S, 2008. Traditional Herbal Medicines used for the treatment of Diabetes among two major tribal groups in South Tamil Nadu, India.Ethno Botanical Leaflet, 47:389-394.

      [37] Fayyaz A., Asbi B.A., Ghazali H.M., Che Man Y.B. and Jinap S. 1994. “Purification and molecular properties of papaya pectinesteraseâ€, Food Chem., Vol.49, 373-378.http://dx.doi.org/10.1016/0308-8146(94)90007-8.

      [38] Biresh, K, Jain Devananda, BannergeeAngush, 2011. New drug delivery system.IJRAP 2(5), 1513-1517.

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  • How to Cite

    Selvaraj, V., M., A., V., B., Arjunan, S., Perumal, S., & K., V. (2016). Phytochemical screening, FT-IR analysis and antimicrobial activity of Wattakaka volubilis. International Journal of Biological Research, 4(2), 165-169. https://doi.org/10.14419/ijbr.v4i2.6457