Evaluation of major nutritional components and phytochemical screening of four different Bangladeshi branded unifloral honey and their brine shrimp toxicity study

  • Authors

    • Md. Mahmodul Hasan Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong (USTC)
    • Md.Jahirul Islam Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong (USTC)
    • Subodh Kumar Sarkar Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong (USTC)
    • Nurul Absar Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong (USTC)
    2016-09-28
    https://doi.org/10.14419/ijbr.v4i2.6670
  • Unifloral Honey, Biochemical Analysis, Sugar, Cytotoxic Effect.

  • Abstract

    The research work was conducted to investigate the biochemical composition of four different Bangladeshi branded unifloral honey. The honey from Litchi (Litchi chinensis), Black cumin (Nigella sativa), Coriander (Coriandrum sativum), Mustard (Brassica campestris) were used. We investigated color, pH, Moisture, Dry matter ,Specific gravity, Total sugar, Reducing sugar, Non reducing sugar, Crude protein, Water soluble protein, Lipid, Ash, Total Carbohydrate, Metabolizable energy, Vitamin C and minerals content. The result revealed the presence of nutrient constituent among the varieties comprising pH(3.47 to 4.06),specific gravity(1.32 to 1.37),dry matter(85.2 to 86.6%), moisture(13.4 to 14.7%),ash(0.64 to 0.66%),lipid(0.14 to 0.16%),crude protein(0.63 to 0.73%), total carbohydrate(83.70 to 85.18%), metabolizing energy(2763.59 to 2808.95Kcal/Kg),total sugar(77.36 to 81.2%),reducing sugar(60.5 to 63.03%),non-reducing sugar(16.61 to 18.17%),vitamin C (4.63 to 6.36mg%), and minerals such as Calcium(6.36 to 7.87mg%) and Iron(1.62 to 1.89mg%) respectively. Moreover, alkaloids, flavonoids found in all varieties but only polyphenols was absent in Black cumin varieties. In the cytotoxic activity studies, LC50 values were obtained in the range of 4800 to 4966.7µg/ml.

  • References

    1. [1] Arriaga E, Navarro-Calvo AL, Díaz-Carbajal E, (2011). Botanical characterisation of Mexican honeys from a subtropical region (Oaxaca) based on pollen analysis. Grana; 50: 40-45. http://dx.doi.org/10.1080/00173134.2010.537767.

      [2] Al-Mamary, M., Al-Meeri, A., & Al-Habori, M. (2002). Antioxidant activities and total phenolics of different types of honey. Nutrition Research, 22, 1041–1047. http://dx.doi.org/10.1016/S0271-5317(02)00406-2.

      [3] Alvarez-Suarez, J.M., Tulipani, S., Romandini, S., Bertoli, E., Battino, M., (2010). Contribution of honey in nutrition and human health: a review. Mediterranean Journal of Nutrition and Metabolism 3, 15–23. http://link.springer.com/article/10.1007/s12349-009-0051-6. http://dx.doi.org/10.1007/s12349-009-0051-6.

      [4] AOAC. (2005). Official Method of Analysis. Ash Analysis 923.03. The Official Methods of Analysis of AOAC International. 18th Ed. Current through revision, (2010).

      [5] AOAC. (2005). Official Method of Analysis. Protein Analysis 984.13. The Official Methods of Analysis of AOAC International. 18th Ed. Current through revision, (2010).

      [6] Alvarez,Liset Maldonado.,(2011).Honey proteins and their interaction with polyphenols. MS thesis. Brock University, page-49. http://biblioteca.versila.com/10246061.

      [7] Bogdanov S, Jurendic T, Sieber R, Gallmann P.,(2008) Honey for nutrition and health: a review. J Am CollNutr; 27: 677-689.http://dx.doi.org/10.1080/07315724.2008.10719745.

      [8] Beretta G, Gelmini F, Lodi V, Piazzalunga A, MaffeiFacino R., (2010) Profile of nitric oxide (NO) metabolites (nitrate, nitrite and N-nitroso groups) in honeys of different botanical origin: nitrate accumulation as index of origin, quality and of therapeutic opportunities. J Pharm Biomed Anal; 53: 343-349.

      http://dx.doi.org/10.1016/j.jpba.2010.04.010.

      [9] Bosi G, Battalglini M., (1978). Gas chromatographic analysis of free and protein amino acids in some unifloral honeys. Journal of Apicultural Research; 17: 152–66. https://doi.org/10.1080/00218839.1978.11099920.

      [10] Basualdo, C., Sgroy, V., Finola, M.S., Marioli, J.M., (2007). Comparison of the antibacterial activity of honey from different provenance against bacteria usually isolated from skin wounds. Veterinary Microbiology 124, 375–381. https://doi.org/10.1016/j.vetmic.2007.04.039.

      [11] Badet, C. and Quero, F. (2011).The in vitro effect of manuka honeys ongrowth and adherence of oral bacteria. Anaerobe, 17, 19-22. http://dx.doi.org/10.1016/j.anaerobe.2010.12.007.

      [12] Bessey, O.A. and C.G.King., 1933.The distribution of vitamin-C in plant and animal tissue and its determination, J.Biol.Chem, 103:687.

      [13] Bligh EG and Dyer W (1959) Total Lipid Extraction and Purification. Can. J.Biochem. Physiol. 37: 911. http://dx.doi.org/10.1139/o59-099.

      [14] Ceylan, O., Uganda, A., Isılogl, M. and Ozcan, F. (2013). Evaluation of the Antibacterial, Antibiofilm, Antioxidant, And Cytotoxic Effects Of Some Turkish Honeys, Apimondia to, XXXXIII International Apicultural Congress, 29 september-04 Octab of 2013, Kyiv, Ukraine.

      [15] Estevinho, L., Prereira, A.P., Moreira, L., Dias, L.G., Pereira, E., (2008). Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey. Food and Chemical Toxicology 46, 3774–3779. http://dx.doi.org/10.1016/j.fct.2008.09.062.

      [16] Edeoga, H.O., D.E. Okwu and B.O. Mbaebie, (2005). Phytochemical constituents of some Nigeria medicinal plants. Afr. J. Biotechnol., 4(7): 685-688. http://dx.doi.org/10.5897/AJB2005.000-3127.

      [17] Farida Iftikhar,Rashid Mahmood, Noor Islam, Ghulam Sarwar,M.Ashif Masood, Hammad Shafiq.,(2014). Physicochemical analysis of honey samples collected from local markets of Rawalpindi and Islamabad,Pakistan. American journal of Biochemistry, 4(2):35-40.

      [18] FAO/WHO/IAEA, (1996). Trace Elements in Human Nutrition and Health, WHO,Geneva.

      [19] Frankel, S., Robinson, G.E., Berenbaum, M.R., (1998). Antioxidant capacity and correlation characteristics of 14 unifloral honeys. Journal of Apiculture Research 37, 27–31. http://dx.doi.org/10.1080/00218839.1998.11100951.

      [20] Gheldof N, Wang XH, Engeseth NJ. (2002). Identification and quantification of antioxidant components of honeys from various floral sources. J Agric Food Chem; 50: 5870-5877. http://www.ncbi.nlm.nih.gov/pubmed/12358452. http://dx.doi.org/10.1021/jf0256135.

      [21] Gomes, S., Dias, L.G., Moreira, L.L., Rodrigues, P., Estevinho, L., (2010). Physicochemical, microbiological and antimicrobial properties of commercial honeys from Portugal. Food and Chemical Toxicology 48, 544–548. http://dx.doi.org/10.1016/j.fct.2009.11.029.

      [22] G.L.Miller, (1959); Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31(3):426-428. http://dx.doi.org/10.1021/ac60147a030.

      [23] Havsteen BH (2002) Biochemistry and Medical Significance of the flavonoids, Pharmacol Ther. 96(2-3):67 – 202. http://dx.doi.org/10.1016/S0163-7258(02)00298-X.

      [24] Huidobro J. F and Simal J., (1984). Determinacion del color y de la tubidez en la mieles. Anal. Bromatol. 36(2), 225-245.

      [25] Jaganathan SK, Mandal M., (2009) Antiproliferative effects of honey and of its polyphenols: A review.J Biomed Biotechnol; article ID 830616. http://dx.doi.org/10.1155/2009/830616.

      [26] J.Jayarman, (1981); Laboratory manual in Biochemistry, 1st Ed., Wiley Eastern Ltd.New Delhi, 75.

      [27] Jaganathan, S. K, Mandal, S. M., Jana, S. K., Das, S.; Mandal, M. (2010). Studies on the phenolic profiling, anti-oxidant and cytotoxic activity of Indian honey: in vitro evaluation. Natural Products Research 24 (14):1295-306. http://dx.doi.org/10.1080/14786410903184408.

      [28] Kucuk, M., Kolaylı, S., Karaoglu, S., Ulusoy, E., Baltacı, C., Candan, F., (2007). Biological activities and chemical composition of three honeys of different types from Anatolia. Food Chemistry 100, 526–534. http://dx.doi.org/10.1016/j.foodchem.2005.10.010.

      [29] Kalimuddin, M., A., (1976). Text Book of Practical Physics (4th Ed.) Bangladesh, 84.

      [30] Lowry, O.H., Rosebrough N.J, Farr A.L and Randall R.L, (1951).Protein measurement with the folin phenol reagent. J.Biol.Chem, 183:265-275.

      [31] Lodhi GN, Daulat Singh and Ichhponani JS (1976). Variation in nutrient content of feeding stuffs rich in protein and reassessment of the chemical method for metabolizable energy estimation for poultry. Journal of Agricultural Science, 86(2): 293-303 http://dx.doi.org/10.1017/S0021859600054757.

      [32] M Asaduzzaman, M Sohanur Rahman, Sirajam Munira, M Muedur Rahman, Minarul Islam, Mhadi Hasan, M Abdul Hai Siddique, (2015); Analysis of biochemical composition of honey and its anti-oxidant, phytochemical and anti-bacterial properties. J.Biomedical and Pharmaceutical Research 4(4), 69-81.

      [33] Meyer BN, Ferringni NR, Puam JE, Lacobsen LB, Nicols DE, McLaughilin JL. (1982). Brine Shrimp: A convenient general bioassay for active constituents. Planta Med. 45: 31-32. http://dx.doi.org/10.1055/s-2007-971236.

      [34] M.Ibrahim Khalil, Md.Abdul Motalib, A.S.M.Anisuzzaman,Zakia Sultana Sathi, M.A.Hye and M.Shahjahan.,(2001).Biochemical analysis of different brands of unifloral honey available at the north region of Bangladesh. Asian network for scientific Information, vol.1 (6):385-388. http://dx.doi.org/10.3923/jms.2001.385.388.

      [35] M Asaduzzaman,M shohanur Rahman, Sirjam Munira, M Muedur Rahman.,(2015).Analysis of biochemical composition of honey and its anti-oxidant, Phytochemical and anti-bacterial properties. J Biomedical and pharmaceutical Research, 4(4), 69-81.

      [36] Mbwambo,Z.H., M.J.Moshi, P.J.Masimba, M.C.Kapingu, R.S. O.Nondo., (2007). Antimicrobial activity and brine shrimp toxicity of extracts of Terminalia brownii roots and stem. BMC complem.AlternativeMed.,Vol.7,10.1186/1472-6882-7-9. http://dx.doi.org/10.1186/1472-6882-7-9.

      [37] Nayik.G.A.,B.N.Dar, V.Nanda.,(2016). Physico-chemical, rheological and sugar profile of different unifloral honeys from Kashmir valley of India. Arabian Journal of Chemistry. http://dx.doi.org/10.1016/j.arabjc.2015.08.017.

      [38] Oddo LP, Piazza MG, Pulcini P. (1999).Invertase activity in Honey. Apidologie; 30: 57- 65. http://dx.doi.org/10.1051/apido:19990107.

      [39] Rauha, J.P., Remes, S., Heinonen, M., Hopia, A., Kahkonen, M., Kujala, T., Pihlaja, K., Vuorela, H., Vuorela, P., (2000). Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. International Journal of Food Microbiology 56, 3–12. http://dx.doi.org/10.1016/S0168-1605(00)00218-X.

      [40] Ranganna,S. (1979). Manual of analysis of fruits and vegetables products. Tata McGraw-Hill Publishing Company Ltd. New Delhi, India, 312 pp.

      [41] Silici, S., Sagdic, O., Ekici, L. (2010). Total phenolic content, antiradical, antioxidant and antimicrobial activities of Rhododendron honeys, Food Chemistry, 12, 238-243. http://dx.doi.org/10.1016/j.foodchem.2009.11.078.

      [42] Taormina, P.J., Niemira, B.A., Beuchat, L.R., (2001). Inhibitory activity of honey against foodborne pathogens as influenced by the presence of hydrogen peroxide and level of antioxidant power. International Journal of Food Microbiology; 69, 217–225. http://dx.doi.org/10.1016/S0168-1605(01)00505-0.

      [43] Truchado, P., Ferreres, F., & Tomas-Barberan, F. A. (2009). Liquid chromatography–tandem mass spectrometry reveals the widespread occurrence of flavonoid glycosides in honey, and their potential as floral origin markers. Journal of Chromatography, A, 1216, 7241–7248. http://dx.doi.org/10.1016/j.chroma.2009.07.057.

      [44] Vogel,A.I., (1978).Vogel's Text book of Quantitative inorganic Analysis (4th Ed.) Longman Group Limited, England, pp: 741, 787, 808.

      [45] Wang J, Li QX., (2011) .Chemical composition, character-ization, and differentiation of honey botanical and geographical origins. Adv Food Nutr Res; 62: 89-137. http://dx.doi.org/10.1016/B978-0-12-385989-1.00003-X.

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  • How to Cite

    Hasan, M. M., Islam, M., Sarkar, S. K., & Absar, N. (2016). Evaluation of major nutritional components and phytochemical screening of four different Bangladeshi branded unifloral honey and their brine shrimp toxicity study. International Journal of Biological Research, 4(2), 227-231. https://doi.org/10.14419/ijbr.v4i2.6670

    Received date: 2016-08-30

    Accepted date: 2016-09-22

    Published date: 2016-09-28