Isolation, fractionation, identification and bioassay studies of the pheromone of the maconellicoccus hirsutus (green, 1908)

  • Authors

    • Mamata Deb
    • Dolly Kumar Department of Zoology,Faculty of Science,The M. S. University of Baroda, Vadodara - 390002, Gujarat
    2016-11-21
    https://doi.org/10.14419/ijbr.v4i2.6801
  • Attractive Index, Bioassay Studies, GC-MS, Maconellicoccus Hirsutus, Phenolic Component.

  • Abstract

    Maconellicoccus hirsutus (Green, 1908) is one of the invasive pests known to infest the agricultural crops of Vadodara, Gujarat. Large amount of pesticides are in use to control this pest which ultimately pose threat to both environment and mankind. So eco-friendly measures are required to limit the invasion of this pest. Hence the objective is to isolate the pheromone from Maconellicoccus hirsutus to uncover its different components through GC-MS along with the bioassay studies. Pheromone was isolated from the species through adsorbent method, and the volatiles were then subjected to GC-MS for fractionation. Crude extracts was further employed for behavioral bioassay to identify the nature of the pheromone. Combined result of GC and GC-MS indicated the presence of phenolic component in the extract. Additionally, observations from bioassay studies confirmed it as sex pheromone where attractive index of males towards volatiles was higher (0.88) than the females. Hence, identification of different pheromonal components will be helpful in synthesizing chemical analogues and their use in controlling the concerned pest in an eco-friendly way.

  • References

    1. [1] Aida, H. M., Moussa, S. F., Abo-Ghalia, A. H., Ahmed, S. A. (2010). Efficiency of certain insecticides on the population(s) of the pink hibiscus mealybug Maconellicoccus hirsutus (Green) and their natural enemies under the field condition in Ismailia governorate. Egypt. Acad. J. biolog. Sci., 2:11- 17.

      [2] Arai, T. (2000). The existence of sex pheromone of Pseudococcus cryptus Hempel (Homoptera: Pseudococcidae) and a simple bioassay. Applied entomology and zoology, 35: 525-528. https://doi.org/10.1303/aez.2000.525.

      [3] Arai, T. (2002). Attractiveness of sex pheromone of Pseudococcus cryptus Hempel (Homoptera: Pseudococcidae) to adult males in a citrus orchard. Applied entomology and zoology, 37: 69-72. https://doi.org/10.1303/aez.2002.69.

      [4] Arai, T., Sugie, H., Hiradate, S., Kuwahara, S., Itagaki, N., Nakahata, T. (2003). Identification of a sex pheromone component of Pseudococcus cryptus. Journal of chemical ecology, 29: 2213-2223. https://doi.org/10.1023/A:1026214112242.

      [5] Aristizabal, L. F., Mannion, C., Bergh, C., Arthurs, S. (2012). Life history of pink hibiscus mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae) on three Hibiscus rosa-sinensis cultivars. Florida Entomologist, 95: 89-94. https://doi.org/10.1653/024.095.0114.

      [6] Bhosle, B. B., Sharma, O. P., More, D. G., Bhede, B. V., & Bambawale, O. M. (2009). Management of mealybugs (Phanacoccus solenopsis) in rainfed cotton (Gossypium hirsutum). Indian Journal of Agricultural Sciences, 79(3): 199-202.

      [7] Bierl-Leonhardt, B. A., Moreno, D. S., Schwarz, M., Fargerlund, J. Plimmer, J. R. (1981). Isolation, identification and synthesis of the sex pheromone of the citrus mealybug, Planococcus citri (Risso). Tetrahedron Letters, 22: 389-392. https://doi.org/10.1016/0040-4039(81)80107-4.

      [8] Bierl-Leonhardt, B. A., Moreno, D. S., Schwarz, M., Forster, H. S., Plimmer, J. R., DeVilbiss, E. D. (1982). Isolation, identification, synthesis, and bioassay of the pheromone of the Comstock mealybug and some analogs. Journal of chemical ecology, 8: 689-699. https://doi.org/10.1007/BF00988310.

      [9] Butcher, F. G. (1958). A Preliminary Report on Armored Scale Insects in Dade County. Florida State Horticultural Society, 362-365.

      [10] Carlson, D. A., Mayer, M. S., Silhacek, D. L., James, J. D., Beroza, M., Bierl, B. A. (1971). Sex attractant pheromone of the house fly: isolation, identification and synthesis. Science, 174: 76-78. https://doi.org/10.1126/science.174.4004.76.

      [11] Daane, K. M., Cooper, M. L., Triapitsyn, S. V., Walton, V. M., Yokota, G. Y., Haviland, D. R., ... Wunderlich, L. R. (2008). Vineyard managers and researchers seek sustainable solutions for mealybugs, a changing pest complex. California Agriculture, 62: 167-176. https://doi.org/10.3733/ca.v062n04p167.

      [12] Daane, K. M., Almeida, R. P., Bell, V. A., Walker, J. T., Botton, M., Fallahzadeh, M. Zaviezo, T. (2012). Biology and management of mealybugs in vineyards. In Arthropod Management in Vineyards: (pp. 271-307). Springer Netherlands. https://doi.org/10.1007/978-94-007-4032-7_12.

      [13] De Alfonso, I., Hernandez, E., Velazquez, Y., Navarro, I. Primo, J. (2012). Identification of the sex pheromone of the mealybug Dysmicoccus grassii Leonardi. Journal of agricultural and food chemistry, 60: 11959-11964. https://doi.org/10.1021/jf304065d.

      [14] Deb, M., and Kumar, D. (2016). Mealy-Ant Association, Distribution and Extent of Damage Caused by Maconellicoccus hirsutus (Hemiptera: Pseudococcidae) on its Various Host Plants in Vadodara, Gujarat. International Journal of Science and Research, 5(4): 1249-1254. https://doi.org/10.21275/23197064.

      [15] Douglass R. Miller., 1999. Identification of the Pink Hibiscus Mealybug, Maconellicoccus hirsutus (Green) (Hemiptera: Sternorrhyncha:Pseudococcidae). Insecta Mundi (paper339), 13: 189 – 203.

      [16] Fall, Y., Van Bac, N., Langlois, Y. (1986). Synthesis of the pheromone of the Comstock mealybug via a sila-cope elimination. Tetrahedron letters, 27: 3611-3614. https://doi.org/10.1016/S0040-4039(00)84862-5.

      [17] Hinkens, D. M., McElfresh, J. S., Millar, J. G. (2001). Identification and synthesis of the sex pheromone of the vine mealybug, Planococcus ficus. Tetrahedron Letters, 42: 1619-1621. https://doi.org/10.1016/S0040-4039(00)02347-9.

      [18] Ho, H. Y., Hung, C. C., Chuang, T. H. Wang, W. L. (2007). Identification and synthesis of the sex pheromone of the passionvine mealybug, Planococcus minor (Maskell). Journal of chemical ecology, 33: 1986-1996. https://doi.org/10.1007/s10886-007-9361-7.

      [19] Ho, H. Y., Su, Y. T., Ko, C. H., Tsai, M. Y. (2009). Identification and Synthesis of the Sex pheromone of the Madeira mealybug, Phenacoccus madeirensis Green. Journal of Chemical Ecology, 35: 724-732. https://doi.org/10.1007/s10886-009-9649-x.

      [20] Hodges, A., Hodges, G. (2006). Pink hibiscus mealybug identification. Plant Health Progress, 1-7. https://doi.org/10.1094/PHP-2006-0414-01-DG.

      [21] Kairo, M. T., Pollard, G. V., Peterkin, D. D., Lopez, V. F. (2000). Biological control of the hibiscus mealybug, Maconellicoccus hirsutus Green (Hemiptera: Pseudococcidae) in the Caribbean. Integrated Pest Management Reviews, 5: 241-254. https://doi.org/10.1023/A:1012997619132.

      [22] Marsaro Junior, A. L., Peronti, A. L. B. G., Penteado-Dias, A. M., Morais, E. G. F., Pereira, P. D. S. (2013). First report of Maconellicoccus hirsutus (Green, 1908) (Hemiptera: Coccoidea: Pseudococcidae) and the associated parasitoid Anagyrus kamali Moursi, 1948 (Hymenoptera: Encyrtidae), in Brazil. Brazilian Journal of Biology, 73: 413-418.

      [23] Millar, J. G., Chinta, S. P., McElfresh, J. S., Robinson, L. J., Morse, J. G. (2012). Identification of the sex pheromone of the invasive scale Acutaspis albopicta (Hemiptera: Diaspididae), arriving in California on shipments of avocados from Mexico. Journal of Economic Entomology, 105: 497-504. https://doi.org/10.1603/EC11366.

      [24] Millar, J. G. and Midland, S. L. (2007). Synthesis of the sex pheromone of the obscure mealybug, the first example of a new class of monoterpenoids. Tetrahedron letters, 48: 6377-6379. https://doi.org/10.1016/j.tetlet.2007.06.169.

      [25] Moghaddam, M. (2006). The mealybugs of southern Iran (Hem: Coccoidea: Pseudococcidae). Journal of Entomological Society of Iran, 26: 1-11.

      [26] Nagrare, V. S., Kranthi, S., Biradar, V. K., Zade, N. N., Sangode, V., Kakde, G., Kranthi, K. R., (2009). Widespread infestation of the exotic mealybug species, Phenacoccus solenopsis (Tinsley) (Hemiptera: Pseudococcidae), on cotton in India. Bulletin of entomological research, 99: 537-541. https://doi.org/10.1017/S0007485308006573.

      [27] OEPP/EPPO. (2005). Data sheets on quarantine pests Maconellicoccus hirsutus. Bull OEPP/EPPO Bull., 35: 413-415.

      [28] Roltsch, W. J., Meyerdirk, D. E., Warkentin, R., Andress, E. R., Carrera, K. (2006). Classical biological control of the pink hibiscus mealybug, Maconellicoccus hirsutus (Green), in southern California. Biological Control, 37: 155-166. https://doi.org/10.1016/j.biocontrol.2006.01.006.

      [29] Sagarra, L. A., Peterkin, D. D. (1999). Invasion of the Caribbean by the hibiscus mealybug, Maconellicoccus hirsutus Green (Homoptera: Pseudococcidae). Phytoprotection, 80: 103-113. https://doi.org/10.7202/706185ar.

      [30] Sagarra, L. A., Vincent, C. H. A. R. L. E. S., Stewart, R. K. (2000). Fecundity and survival of Anagyrus kamali (Hymenoptera: Encyrtidae) under different feeding and storage temperature conditions. European Journal of Entomology, 97: 177-181. https://doi.org/10.14411/eje.2000.034.

      [31] Sagarra, L. A., Vincent, C., Stewart, R. K. (2001). Suitability of nine mealybug species (Homoptera: Pseudococcidae) as hosts for the parasitoid Anagyrus kamali (Hymenoptera: Encyrtidae). Florida Entomologist, 84: 112-116. https://doi.org/10.2307/3496669.

      [32] Patil, S. V., Patil, C. D., Salunkhe, R. B., Maheshwari, V. L., & Salunke, B. K. (2011). Studies on life cycle of mealybug, Maconellicoccus hirsutus (Green)(Hemiptera: Pseudococcidae), on different hosts at different constant temperatures. Crop Protection, 30(12): 1553-1556. https://doi.org/10.1016/j.cropro.2011.08.010.

      [33] Sharma, O.P., Bhosle, B.B., Deshpande, G.D., Bombawale, O.M., Jagpat, G.P. Bhede, B.V., More, D.G., Patange, N.R. (2008). Pest records on transgenic cotton in the marathwada region. Indian journal of plant protection, 36: 186-191.

      [34] Singh, A. (2012) Adoption of Bio-rational alternatives to pesticides in selected agricultural crops. P.hD. Thesis, the Maharaja Sayajirao University of Baroda, Vadodara, Gujarat (India).

      [35] Singh, A., Kumar, D. (2015). Studies on Presence of Semiochemicals from Phenacoccus solenopsis (Tinsley). International Journal of Pharma and Biosciences, 6: 326- 332

      [36] Sugie, H., Teshiba, M., Narai, Y., Tsutsumi, T., Sawamura, N., Tabata, J. &Hiradate, S. (2008). Identification of a sex pheromone component of the Japanese mealybug, Planococcus kraunhiae (Kuwana). Applied entomology and zoology, 43(3): 369-375. https://doi.org/10.1303/aez.2008.369.

      [37] Tabata, J. and Ichiki, R. T. (2015). A new lavandulol-related monoterpene in the sex pheromone of the grey pineapple mealybug, Dysmicoccus neobrevipes. Journal of Chemical Ecology, 41: 194-201. https://doi.org/10.1007/s10886-015-0545-2.

      [38] Tamaki, Y., Noguchi, H., &Yushima, T. (1973). Sex pheromone of Spodoptera litura (F.) (Lepidoptera: Noctuidae): Isolation, identification, and synthesis. Applied Entomology and Zoology, 8: 200-203. doi: http://doi.org/10.1303/aez.8.200

      [39] Waghole, R. J. and Naik, D. G. (2014). Attractant formulations for the management of grape mealy bug Maconellicoccus hirsutus (Green). JBiopest 7:83-88.

      [40] Williams, D.J. (1996). A brief account of the Hibiscus mealybug Maconellicoccus hirsutus (Hemiptera: Pseudococcidae), a pest of agriculture and horticulture, with descriptions of two related species from southern Asia. Bulletin of Entomological Research, 86: 617 - 626. https://doi.org/10.1017/S0007485300039420.

      [41] Zhang, A., Amalin, D., Shirali, S., Serrano, M. S., Franqui, R. A., Oliver, J. E., Lapointe, S. L. (2004). Sex pheromone of the pink hibiscus mealybug, Maconellicoccus hirsutus, contains an unusual cyclobutanoid monoterpene. Proceedings of the national Academy of Sciences of the United States of America, 101: 9601-9606. https://doi.org/10.1073/pnas.0401298101.

      [42] Zhang, A., Nie, J. (2005). Enantioselective synthesis of the female sex pheromone of the pink hibiscus mealybug, Maconellicoccus hirsutus. Journal of agricultural and food chemistry, 53: 2451-2455. https://doi.org/10.1021/jf048198k.

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  • How to Cite

    Deb, M., & Kumar, D. (2016). Isolation, fractionation, identification and bioassay studies of the pheromone of the maconellicoccus hirsutus (green, 1908). International Journal of Biological Research, 4(2), 299-306. https://doi.org/10.14419/ijbr.v4i2.6801

    Received date: 2016-09-27

    Accepted date: 2016-10-29

    Published date: 2016-11-21