Salivary and serum malondialdehyde levels in type 2 diabetes mellitus with dental caries


  • Mithra N Hegde HOD conservative dentistry and endodontics
  • Nireeksha Shetty postgraduate studentconservative dentistry and endodonticsA.B.shetty memorial instituite of dental sciences
  • Preethesh Shetty ostgraduate studentconservative dentistry and endodonticsA.B.shetty memorial instituite of dental sciences





Dental Caries, Diabetes Mellitus, Malondialdehyde, Oxidative Stress, Saliva


The aim of the study was to evaluate the salivary and serum Malondialdehyde levels in type two diabetes mellitus patients with dental carries. The study was conducted among 80 individual, which included experimental and control group with 40 individuals each divided based on presence and absence of type two diabetes mellitus and DMFT score. The results showed significant increase in salivary and serum Malondialdehyde in type two diabetes mellitus patients with dental caries compared to the control group which comprised of indi-viduals in absence of caries and no diabetes mellitus. Thus, confirming increased oxidative stress in type two diabetes mellitus individuals.


[1] MAbayon (2007)National diabetic statistics, U.S.dept of health and human services, National Instituite of diabetic and digestive and kidney diseases, National instituite of health;p:1-24.

[2] Stumvoli M, Goldestain B and Timon WH (2005).Type 2 diabetes; Principles of pathogenesis and therapy lancet; 65; 333-346.

[3] Bergman RN lilly, Lilly L. (1989) toward physiological understanding of glucose tolerance.Minimal model approach diabetes.Dec; 38 (12): 15112-27.

[4] Vadde R, rama J. (2007) Evaluation of oxidative stress in insulin dependent diabetes mellitus (IDDM) patients. Diagnostic pathology; 2:22.

[5] Wolff SP (1993) Diabetes Mellitus and free redicals.Free radicals,transition metals and oxidative stress in the etiology of diabetes mellitus and complications.Br med bull;49:642-652.

[6] Taylor GW, Burt BA, Becker MP, Genco RJ, Shlossman M (1998). Glycemic control and alveolar bone loss progression in type 2 diabetes. Ann Periodontol. Jul; 3 (1):30-9.

[7] Grossi SG. (2001) Treatment of periodontal disease and control of diabetes: an assessment of the evidence and need for future research. Ann Periodontol. Dec; 6 (1):138-45.

[8] Copenhagen, Denmark: Blackwell Monksgaard; (2003).Dental caries: the disease and its clinical management.

[9] Selwitz RH, Ismail AI, Pitts NB (2007). Dental caries. Lancet. Jan 6; 369 (9555):51-9.

[10] Tenovuo J, Alanen P, Larjava H, Viikari J, Lehtonen OP(1986).Oral health of patients with insulin-dependent diabetes mellitus. Scand J Dent Res. Aug; 94 (4):338-46.

[11] Caufield PW, Griffen AL (2000). Dental caries. An infectious and transmissible disease. Pediatrian Clinic North Am. Oct; 47 (5): 1001-19.

[12] Marnett LJ (2002) Lipid peroxidation-DNA damage by Malondialdehyde .Mutatation Research 4224: 83-95.

[13] Classification and diagnosis of diabetes. American Diabetes association (2015).Diabetes Care January vol. 38 no. Supplement 1 S8-S16.

[14] Armstrong, D.; Browne, R. (1994) Free Rad. Diagnostic Medicine, 366; 43-58.

[15] Botsoglou, N.A. J.Agric. (1994) Food Chem., 42; 1931-1937.

[16] Yagi, K. Free Rad. Antiox. Prot., (1998), 108; 101-106.

[17] Navazesh M(1993).Journal of dental reaserach.Methods of collecting saliva, Pub med index:821508

[18] Surdacka A,Ciezka E, PiorunskaStolzmann M,(2011).Relation of salivary antioxidant status and cytokinelevels to clinical parameters of oral health in pregnantwomen with diabetes. Arch Oral Biol; 56:428-436

[19] Todorovic T, Dozic I, Barrero MV, Ljuskovic B, Pejovic J, Marjanovic M (2006) Salivary enzymes and periodontal disease. Med Oral Patol Oral Cir Bucal.; 11:E115-9

[20] Mithra N. Hegde , Divya Tahiliani , Shilpa S. Shetty ,Darshana Devadiga (2014) .Salivary Electrolytes a biomarker in caries active type 2 diabetes mellitus – a comparative NUJHS Vol. 4, No.3, September, 85-88.

[21] Mata AD, Marques D, Rocha S, Francisco H, Santos C, Mesquita MF, et al. Effects of diabetes mellitus on salivary secretion and its compositionin the human. Mol Cell Biochem. 2004; 261:137-42.

[22] Cerielo A. (2000) Oxidative stress and glycemic regulation. Metabolism; 49 (2) supplememnt1:27-29.

[23] Maritim AC, Sanders RA, (2003) Journal of Biochemistry and Molecular Toxicology. Diabetes, oxidative stress, and antioxidants: a review ;17(1):24-38

[24] Ayaz K Mallick, Ravindra Maradi, Vivek R. Joshi,Gaurav Shorey, Marya Ahsan(2011). Study on Malondialdehyde as a marker of lipid peroxidation in male and female patients with Type-2 Diabetes Mellitus. International Journal of Pharmaceutical sciences Review and Research May-June 2011: vol8, issue 2. Article 033.198-201.

[25] Meenakshi Thakur and Dinesh Javarappa (2014) Adenosine Deaminase and Malondialdehyde Levels in Type-2 Diabetes Mellitus – a Short Study Volume 14 Issue 4.

[26] Carda C, Mosquera-Lloreda N, Salom L, Gomez de Ferraris ME, Peydro A. (2006) Structural and functional salivary disorders in type 2 diabetic patients. Med Oral Patol Oral Cir Bucal ; 11:E309-14.

[27] Al-Rawi NH (2011) Oxidative stress, antioxidant status and lipid profile in the saliva of type 2 diabetics. Diab Vasc Dis Res.; 8:22-8.

[28] Bigagli E, Raimondi L, Mannucci E, Colombi C, Bardini G, Rotella CM (2011) Lipid and protein oxidation products, antioxidant status and vascular complications in poorly controlled type 2 diabetes. British Journal of Diabetes Vascular Disorders; 12:33-9.

View Full Article: