Assessment of quantitative relationship between blood and PRF for periodontal surgeries

 
 
 
  • Abstract
  • Keywords
  • References
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  • Abstract


    Aim: The aim of study is to measure the amount of PRF obtained from different quantity of blood withdrawn from patients & the amount of PRF and its use in different periodontal surgical procedures.

    Materials and method: 15 subjects undergoing regenerative periodontal surgical therapy in whom use of PRF was indicated were included in the study. 15 subjects were divided into 3 three groups. In group A: 5ml of blood was withdrawn from 5 subjects. In group B: 10ml of blood was withdrawn from 5 subjects. In group C: 12ml of blood was withdrawn from 5 subjects. The steps involved were as follows:

    1)      Blood specimen was withdrawn from the patient,

    2)      The blood specimen was immediately placed in the centrifuge and was allowed to spin for 10mintes at 3000RPM,

    Weight of obtained PRF was measured by using weighing scale and also length, depth & width was measured by using UNC 15 probe.

    Result and Conclusion: The amount of PRF obtained depends upon the quantity of blood withdrawn. Thus, result of the present study is helpful to the operator to decide the quantity of blood to be withdrawn for particular patient to obtain desired amount of PRF.


  • Keywords


    Class Iifurcation Defects; Intrabony; PRF, Reccesion; Vestibuloplasty

  • References


      [1] Giannobile WV. The potential role of growth and differentiation factors in periodontal regeneration. J Periodontol 1996;67:545-553

      [2] Greenwell H. Committee on research, science and therapy, American Academy of Periodontology. Position paper: guidelines for periodontal therapy. J Periodontol 2001; 72:1624–1628. https://doi.org/10.1902/jop.2001.72.11.1624.

      [3] Ross R et al. A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro. Proc Natl Acad Sci U S A. 1974; 71: 1207–1210 https://doi.org/10.1073/pnas.71.4.1207.

      [4] Giannobile WV. Periodontal tissue engineering by growth factors. Bone 1996; 19(Suppl. 1):23S–37S https://doi.org/10.1016/S8756-3282(96)00127-5.

      [5] V.Gupta et al. “Regenerative potential of platelet rich fibrin in dentistry:literature review,” Asian Journal of Oral Health and Allied Sciences 2011;vol.1,no.1:22–28

      [6] E.Marx et al. “Platelet-rich plasma: growth factor enhancement for bone grafts,” Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, 1998; vol. 85,638–646 https://doi.org/10.1016/S1079-2104(98)90029-4.

      [7] M. Toffler et al. “Introducing Choukroun’s platelet rich fibrin (PRF) to the reconstructive surgery milieu,”The Journal of Implant and Advanced Clinical Dentistry, 2009; vol. 1, no. 6, pp. 21–32.

      [8] T. R. Appel et al. “Comparison of three different preparations of platelet concentrates for growth factor enrichment,” Clinical Oral Implants Research, 2002; vol. 13: no. 5, pp. 522–528. https://doi.org/10.1034/j.1600-0501.2002.130512.x.

      [9] Choukroun J et al: Platelet-rich fibrin (PRF): a second-generation platelet ncentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 101:299–303 https://doi.org/10.1016/j.tripleo.2005.07.012.

      [10] Katti, et al. : Platelet Rich Fibrin in The Treatment of Furcation Defect : A Case Report: A Journal of Clinical Dentistry;March-April 2012; Volume 04 :Issue 04:14-16

      [11] Simonpieri A et al. Current knowledge and perspectives for the use of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in oral and maxillofacial surgery part 2: Bone graft, implant and reconstructive surgery. Curr Pharm Biotechnol. 2012; 13: 1231–1256. https://doi.org/10.2174/138920112800624472.

      [12] Yilmaz D el al. Effect of platelet rich fibrin and beta tricalcium phosphate on bone healing. A histological study in pigs:Acta Cir Bras 2014;29:55-59. https://doi.org/10.1590/s0102-86502014000100009.

      [13] Gassling V el al. ST Platelet-rich fibrin membranes as scaffolds for periosteal tissue engineering. Clin Oral Implants Res 2010; 21:543–549 https://doi.org/10.1111/j.1600-0501.2009.01900.x.

      [14] Chang Y-C et al. Clinical application of platelet-rich fibrin as the sole grafting material in periodontal intrabony defects. J Dent Sci 2011; 6:181–188 https://doi.org/10.1016/j.jds.2011.05.01.

      [15] Pradeep AR & Sharma A. Autologous platelet rich fibrin in the treatment of mandibular degree II furcation defects: a randomized clinical trial. J Periodontol 2011; 82:1396–1403 https://doi.org/10.1902/jop.2011.100731.

      [16] Pradeep AR & Sharma A. Treatment of 3-wall intrabony defects in chronic periodontitis subjects with autologous platelet rich fibrin—a randomized controlled clinical trial. J Periodontol. 2011; 82(12):1705–1712. https://doi.org/10.1902/jop.2011.110075.

      [17] Deodhar AK & Rana RE. Surgical physiology of wound healing: a review. J Postgrad Med 1997; 43:52–56.

      [18] Su CY et al. invitro release of growth factors from platelet rich fibrin (PRF): a proposal to optimize the clinical applications of PRF. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009; 108:56–61. https://doi.org/10.1016/j.tripleo.2009.02.004.

      [19] Raja S et al. Growth factors in periodontal regeneration. Int J Dent Hyg 2009; 7:82–89. https://doi.org/10.1111/j.1601-5037.2009.00380.x.

      [20] Giannobie WV et al. Comparative effects of platelet- derived growth factor-BB and insulin-like growth factor-I, individually and in combination on periodontal regeneration in Macaca fascicularis. J Periodontal Res 1996; 31:301. https://doi.org/10.1111/j.1600-0765.1996.tb00497.x.

      [21] Anilkumar K et al. Platelet-rich-fibrin: a novel root coverage approach. J Indian Soc Periodontol 2009; 13:50–54 https://doi.org/10.4103/0972-124X.51897.

      [22] Aroca S et al. Clinical evaluation of a modified coronally advanced flap alone or in combination with a platelet-rich fibrin membrane for the treatment of adjacent multiple gingival recessions: a 6-month study. J Periodontol 2009; 80:244–252 https://doi.org/10.1902/jop.2009.080253.

      [23] Arunachalam et al. A novel surgical procedure for papilla reconstruction using platelet rich fibrin. Contemp Clin Dent 2012 Oct-Dec; 3(4): 467–470. https://doi.org/10.4103/0976-237X.107443.

      [24] Singhal A et al. Novel surgical procedure for vestibular extension with platelet‑rich fibrin. Int J Oral Health Sci 2015; 5:66-70. https://doi.org/10.4103/2231-6027.171173.

      [25] Kulkarni et al. Platelet‑rich fibrin as an adjunct to palatal wound healing after harvesting a free gingival graft: A case series. J Indian Soc Periodontol 2014; 18:399-402. https://doi.org/10.4103/0972-124X.134591.


 

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Article ID: 7469
 
DOI: 10.14419/ijdr.v5i1.7469




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