In vitro and in vivo studies of anti diabetic effect of khaya senegalensis leaves and bark extracts

  • Authors

    • Marvit Osman Widdat Allah University of Medical Sciences and Technology Faculty of Pharmacy
    • Ayat Ahmed Alrasheid University of Medical Sciences and Technology Faculty of Pharmacy
    • Eltayeb Suliman Elamin
    2019-06-12
    https://doi.org/10.14419/ijpt.v7i1.25340
  • Khaya Senegalensis, Bark, Leaves, Anti Diabetic, Α-Amylase, Rats.

  • Abstract

    Diabetes mellitus in Sudan is one of public health concern since it causes significant mortality and complications for long term. Though conventional drugs are used in the management of diabetes mellitus they are expensive, unavailable and also have numerous side effects. Khaya senegalensis has traditionally used in the management of diabetes. The present study was conducted to examine the In vitro and In vivo anti-diabetic activity of leaves and bark extracts of Khaya senegalensis. The leaves and bark of the plant were extracted with ethanol 96%, and then tested for anti-diabetic activity in a series of in vitro models and a type 2 diabetes model of rats. In vitro bark extract of k.senegalensis showed higher inhibitory activities against the enzyme with IC50 value 226.14 µg/ml. In vivo oral administration of the extracts of the k. senegalensis exhibited decrease in blood sugar level and was found to be time dependent. Bark extract showed strong in vitro and in vivo anti diabetic activity.

     

     

  • References

    1. [1] Bickii, J., Njifutie, N., Foyere, J.A., Basco, L.K., Ringwald, P. (2010). In vitro antimalarial activity of limonoids from Khaya grandifoliola (Meliaceae). J. Ethnopharmacol.69: 27-33. https://doi.org/10.1016/S0378-8741(99)00117-8.

      [2] Danquah, J.A., Appiah, M., Pappinen, A (2012). The effect of African mahogany species on soil chemical properties in degraded dry semideciduous forest ecosystems in Ghana. Int J Agric Biol.14:321–328. https://doi.org/10.14214/df.148.

      [3] Davis, S.N., Granner, D.K. (2001). Insulin, oral hypoglycemic agents and the pharmacology of endocrine pancreas. In: Brunton, L.L., Lazo, J.S., Parker, K.L. (Ed.), Goodman and Gilman’s: The pharmacological basis of therapeutics, 11th ed. (McGraw-Hill Medical Publication Division:New York. 1706-1707.

      [4] DeFronzo, R.A. (2004). Pathogenesis of type 2 diabetes mellitus. Med. Clin. North. Am. 88: 787–835. https://doi.org/10.1016/j.mcna.2004.04.013.

      [5] El Badwi, S.M.A., Bakhiet, A.O., Abdel Gadir, E.H. (2012). Haemato-biochemical effects of aqueous extract of Khaya senegalensis stem bark on gentamicin-induced nephrotoxicity in wistar rats. J. Biol. Sci. 12:361-366. https://doi.org/10.3923/jbs.2012.361.366.

      [6] Hassan, B.A.R. (2013). Overview on Diabetes Mellitus (Type 2). J Chromat Separation Techniq.4:114.

      [7] Ibrahim, M, A., Koorbanally, N. A and Islam, M. S. (2013). Anti-oxidative, α-glucosidase and α-amylase inhibitory activity of Vitex doniana: possible exploitation in the management of type 2 diabetes. Records of Natural Products, 1-21.

      [8] Ibrahim,M.A., Koorbanally, N. A and Islam, M. S. (2013). Anti-oxidative activity and inhibition of key enzymes linked to type 2 diabetes (α-glucosidase and α-amylase) by Khaya senegalensis. Records of Natural Products. 195-200. https://doi.org/10.2478/acph-2014-0025.

      [9] Ijeoma, J.U., Idu, M., Umar, A.H. (1997). Medicinal plant in use by the Fulani traditional herbalist in Yola North and Yola South L. G. A. of Adamawa State. J. Appl. Sci. Manage. 1(1):59-63.

      [10] Kimani, N.L., Njangiru, I.K., Njagi, E.N.M and Orinda, G.O. (2017). Antidiabetic Activity of Administration of Aqueous Extract of Berberis holstii. J Diabetes Metab. 8: 774. https://doi.org/10.4172/2155-6156.1000774.

      [11] Kolawole,O.T., Kolawole ,S.O., Ayankunle, A.A., Olaniran ,O.I. (2012). Anti-hyperglycemic effect of Khaya senegalensis stem bark aqueous extract in wister rats. EJMP. 2(1):66- 73. https://doi.org/10.9734/EJMP/2012/934.

      [12] Maroyi A. (2013). Traditional use of medicinal plants in south‑central Zimbabwe: Review and perspectives. J.Ethnobiol Ethnomed. 9:31. https://doi.org/10.1186/1746-4269-9-31.

      [13] Narkhede, M. B., Ajimire, P. V., Wagh ,A. E., Mohan ,M and Shivashanmugam, A. (2011). In vitro antidiabetic activity of Caesalpina digyna (r.) methanol root extract, Asian journal of plant science and research. 1(2): 101-106.

      [14] Sule, M.S., Abdulraheem, R.B., Aminu, B.M. (2008). Potency of aqueous stem bark extract of Khaya senegalensis against liver diseases in rats. BAJOPAS. 1:29-31. https://doi.org/10.4314/bajopas.v1i1.57508.

      [15] Widdat Allah, M.O., Alrasheid, A.A and Elamin, A.S (2018). Phytochemical Screening, Chemical Composition and Antioxidant Activity of Leaves and Bark Extracts from Khaya senegalensis. Advances in Biochemistry. 6(4):32-38. https://doi.org/10.11648/j.ab.20180604.12.

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  • How to Cite

    Osman Widdat Allah, M., Ahmed Alrasheid, A., & Suliman Elamin, E. (2019). In vitro and in vivo studies of anti diabetic effect of khaya senegalensis leaves and bark extracts. International Journal of Pharmacology and Toxicology, 7(1), 12-16. https://doi.org/10.14419/ijpt.v7i1.25340

    Received date: 2019-01-04

    Accepted date: 2019-04-18

    Published date: 2019-06-12