Antibacterial activity of selected siddha herbo-mineral preparations against clinically isolated enterococcus species


  • shobha K.L K.L Professor Department of Microbiology Melaka Manipal Medical College Manipal Campus Manipal university Manipal
  • Shobha K.L Professor Department of Microbiology Melaka Manipal Medical College,Manipal Campus Manipal University Manipal
  • Akshatha S.J PG Student department of Microbiology Kasturba Medical college,Manipal Manipal University Manipal
  • Revathi Shenoy Department of Biochemistry Kasturba Medical College Manipal Manipal University Manipal
  • Ramachandra L Department of Surgery Kasturba Medical College Manipal Manipal university Manipal
  • Bernitis L PG Student Department Of Microbiology Melaka Manipal Medical college,Manipal Manipal university Manipal





Objective: In siddha medicinal practice the use of plant extracts as well as inorganic natural preparations has a vital role as medicines. The present research in the field of phytomedicine is to evaluate the antibacterial properties of three herbo- mineral siddha drugs (Palakarai parpam, Padikara parpam, Uppu chenduram) on clinically isolated Enterococcus strains.

Materials and Methods: Fifteen bacterial strains isolated from clinical samples were identified as Enterococcus faecalis(7), E. faecieum(8).The susceptibility of the microorganisms to the  siddha drugs (Palakarai parpam, Padikara parpam, Uppu chendooram) were screened by disc diffusion method and the effective drug’s MIC value was calculated by agar dilution method.

Results: Padikara parpam was considered sensitive against the tested Enterococcus faecalis and Enterococcus faecium .Agar dilution was performed with 1%, 0.5%, 0.25% and 0.12% of the drug dilution. MIC was found to be 0.5%.

Conclusion: The drug Padikara parpam contains essential elements which are considered to be good anti-microbial activity against Enterococcus spp. To conclude, the claim in the siddha system of medicine is scientifically validated.


Keywords: Palakarai Parpam, Padikara Parpam, Uppu Chendooram, Siddha Medicine, Antibacterial Activity, Enterococcus species.


Anjan G., Nagesh L., Sapna B (2012). Evaluation of antimicrobial potential of 10% ginger extract against streptococcus mutans, candida albicans and enterococcus faecalis – an in-vitro study. International Journal of Science Innovations and Discoveries. 2 (1). 260-265.

Arjun Ram, Duraisamy Arul Joseph, Selvakumar Balachandar, Vijay Pal Singh. Medicinal plants from Siddha system of medicine useful for treating respiratory diseases. International Journal of Pharmaceuticals Analysis, ISSN: 0975-3079, Volume 1, Issue 2 (2009) pp-20-30.

Arunvanan.M, Sasi.S.K, Mubarak.H, Kanagarajan.A (2013) an overview on anti-diabetic activity of siddha medicinal plants. Asian Journal of Pharmaceutical and Clinical Research .Vol 6, Suppl 2

Austin A. and Jegadeesan M.99. Standardisation of ‘Lingha Chendooram’ – Number 1, a Siddha Drug. Ancient Science of Life Vol. No. XIX

Gopinath R. and Prakash M (2013) Antibacterial activity of three medicinal plants against clinically isolated multidrug resistant Enterococcus faecalis (MDRE). Int.J.Curr.Microbiol.App.Sci 2(2):6-14.

Guzman C.A., Pruzzo C., Lipira G, and Calegari L. (1989) Role of Adherence in Pathogenesis of Enterococcus faecalis Urinary Tract Infection and Endocarditis. Infection and Immunity. p. 1834-1838 Vol. 57, No. 6.

Handral H.K., Pandith A., Shruthi S. D (2012) a review on murraya koenigii: multipotential medicinal plant. Asian Journal of Pharmaceutical and Clinical Research .Vol 5, Suppl 4.

Josephin Sheeba.B and Selva Mohan. T (2012) Antimicrobial activity of Adhatoda vasica against clinical pathogens. Asian Journal of Plant Science and Research. 2 (2):83-88.

Michael J S, Ranjith Singh A. J .A. and Padmalatha C. (2011) Antibacterial potential of some herbo-mineral siddha preparation: An alternative medicine for enteric pathogens. J. Chem. Pharm. Res. 3(3):572-578.

Oli A.K., Raju. S, Rajeshwari, Nagaveni. S and Kelmani C. R (2012) Biofilm formation by Multidrug resistant Enterococcus faecalis (MDEF) originated from clinical samples. J. Microbiol. Biotech. Res. 2 (2):284-288.

Revathy S.S. and Murugesan M (2013) Physico Chemical Analysis and Anti-Microbial Activity of a Siddha Herbo Mineral Drug. Silasathu Paavanai. Int J Pharm Bio Sci .4(2): (P) 215 – 221.

Sathish Kumar M., Rao M.R.K., Ganesan A., Rengasundari G. (2013) Antibacterial Screening of Kodasuri Veeravaippu, A Siddha Salt Preparation. Int. J. Pharm. Sci. Rev. Res., 20(1); nᵒ 23, 140-141.

Sher A (2009) Antimicrobial Activity of Natural Products from Medicinal Plants. Gomal Journal of Medical Sciences, Vol. 7, No. 1.

Shree Devi M.S., Sampath B. and Siva Shankar M.S (2011) Antimicrobial and analgesic activity of traditional Siddha formulation and its ingredients. IJPI’S Journal of Pharmacology and Toxicology, Vol 1:3.

Siva Shankar M.S., Shree Devi M.S., Sampath Kumar B (2013) Phytochemical Analysis and Antimicrobial Activity Studies of Sirukurinjan Ver Powder. Sch. Acad. J. Biosci.; 1(3):102-103.

Uma K.S., Jacob M., Arumugam G., Kalpana S. and Natarajan M (2012) In Vitro Antimicrobial Activity of the Siddha Drugs Seenthil Sarkarai and Nilavembu Kudineer against Leptospira. International Journal of Pharmacy and Pharmaceutical Sciences. Vol 4, Suppl 2.

Verma S. and Singh S.P.(2008) Current and future status of herbal medicines. Veterinary World, Vol.1 (11): 347-350.

View Full Article: