Comparative studies on the effects of lincomycin and bacitracin on hematobiochemical and immunological parameters in broiler chickens


  • Ashraf A. Elkomy Lecturer of Pharmacology Department, Faculty of Veterinary Medicine, Benha University
  • Enas Farag Professor Pharmacology Department, Faculty of Veterinary Medicine, Benha University
  • Elshahat I. Elgharbawy Professor Pharmacology, Pharmacology Department, Animal Health Research Institute (Benha branch)
  • Mohamed Elbadawy Pharmacology Department, Animal Health Research Institute (Benha branch),





Bacitracin, Biochemical, Chickens, Hematological, Immunological, Lincomycin.


A total of 100 one-day-old healthy broiler chicks were used to study the effects of lincomycin and bacitracin on some hematobiochemical and immunological parameters. Chicks were divided into four equal groups, 25 each. The first group was kept as control; the 2nd group was received 0.5 g of lincomycin per liter; the 3rd group was received 100 mg bacitracin per liter and the 4th group was administered both lincomycin and bacitracin, each at the above-mentioned dose. Drugs were given in drinking water for 5 successive days from 20th to 25th day of age. Bodyweight was recorded at the beginning of the experiment and at 1st-day post administration where body performance was recorded. One day post administration, blood samples were collected for estimation of hematobiochemical and immunological alterations. The obtained results revealed that broiler chicks administered lincomycin or bacitracin or both revealed a marked increase in bodyweight, weight gain, phagocytic activity, phagocytic index, erythrocytic count, hemoglobin level, packed cell volume, total leukocytic count, serum total protein, albumin, total globulin, α, β and γ globulin. Furthermore, a significant elevation in malondialdehyde associated with a marked reduction in albumin-globulin ratio, serum total lipid, cholesterol and triglyceride and a significant decrease in catalase and superoxide dismutase, were recorded, compared with the control group. In conclusion, lincomycin and bacitracin either alone or in combination have positive impacts on growth performance, immunological and hematobiochemical parameters of broiler chickens. So, it is recommended to use both drugs as growth promoters in broiler chickens.





[1] Abaza IM, Shehata MA, Shoieb MS & Hassan II (2008). Evaluation of some natural feed additive in growing chicks diets. International Journal of Poultry Science 7, 872-879.

[2] Abdelaziz M (2002) Hand Book of Veterinary Pharmacology 5th edn. Faculty of Vet. Medicine, Zagazig University, Zagazig, Egypt

[3] Abdulrahim SM, Haddadin SY, Hashlamoun EA & Robinson RK (1996). The influence of Lactobacillus acidophilus and bacitracin on layer performance of chickens and cholesterol content of plasma and egg yolk. British poultry science 37, 341-346.

[4] Aboubakr M & Elbadawy M (2016). Efficacy of Flagymox® (amoxicillin and metronidazole combination) in controlling Clostridium perfringens Infection in broiler chickens. World Journal of Pharmacy and Pharmaceutical Sciences 6 (1), 80-95. & doi: 10.20959/wjpps20171-8374

[5] Adel AE (2018). Some Pharmacological Studies on Synbiotics and Phytobiotics. Thesis, Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Egypt.

[6] Ali SA, Hasan KA, Bin Asif H & Abbasi A (2014). Environmental enterococci: I. Prevalence of virulence, antibiotic resistance and species distribution in poultry and its related environment in Karachi. Pakistan Letters in Applied Microbiology 58, 423-432.

[7] Ali SMM (2007). Concurrent Use of Clindamycin with Spectinomycin (Clindaspectin) in Broiler Chicks. Thesis, Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Egypt.

[8] Alikhani A & Salehifar E (2012). An unreported clindamycin adverse reaction: wrist monoarthritis. Iranian Journal of Pharmaceutical Research 11 (3),959-962.

[9] Bergmeyer H-U (1974). Methods of Enzymatic Analysis V2. 2nd edn. Academic Press,

[10] Butaye P, Devriese LA & Haesebrouck F (2003). Antimicrobial growth promoters used in animal feed: effects of less well known antibiotics on gram-positive bacteria. Clinical microbiology reviews 16,175-188

[11] Chan G, Guthrie A, Sivaramalingam T, Wilson J, Vancraeynest D, Moody R & Clark S (2015). A framework for assessing the efficacy of antimicrobials in the control of necrotic enteritis in broiler chickens. The Journal of Applied Poultry Research 24, 246-256

[12] Charleston B, Gate JJ, Aitken IA, Stephan B & Froyman R (1998). Comparison of the efficacies of three fluoroquinolone antimicrobial agents, given as continuous or pulsed-water medication, against Escherichia coli infection in chickens. Antimicrobial Agents and Chemotherapy 42, 83-87.

[13] Cheng HH, Hou WC & Lu ML (2002). Interactions of lipid metabolism and intestinal physiology with Tremella fuciformis Berk edible mushroom in rats fed a high-cholesterol diet with or without Nebacitin. Journal of agricultural and food chemistry 50, 7438-7443.

[14] Chowdhury S, Mandal GP, Patra AK, Kumar P, Samanta I, Pradhan S & Samanta AK (2018). Different essential oils in diets of broiler chickens: 2. Gut microbes and morphology, immune response, and some blood profile and antioxidant enzymes. Animal Feed Science and Technology 236, 39-47.

[15] Cialdella JI, Vavra JJ & Marshall VP (1986). Susceptibility of bacteria to serum lysis or phagocytosis following growth in subinhibitory levels of lincosaminide or spectinomycin related antibiotics. The Journal of antibiotics 39, 978-984.

[16] Dafwang, II, Cook ME & Sunde ML (1987). Interaction of dietary antibiotic supplementation and stocking density on broiler chick performance and immune response. British poultry science 28, 47-55.

[17] Dawkins MS, Donnelly CA & Jones TA (2004). Chicken welfare is influenced more by housing conditions than by stocking density. Nature, 427, 342-344.

[18] Ding YY, Zhang CH, He XL, Huang L & Yin ZJ (2011). Effects of Chinese herbal medicine additives on antioxidant status, serum biochemical parameters and digestive enzymatic activities in weaned piglets. Journal of Animal and Veterinary Advances 10, 2004-2009.

[19] Doumas BT, Bayse DD, Carter RJ, Peters T & Schaffer R (1981). A candidate reference method for determination of total protein in serum. I. Development and validation. Clinical chemistry 27 (10), 1642-1650.

[20] Elbadawy M & Aboubakr M (2017). Efficacy of colimox® (a new combination of amoxicillin and colistin) in the control of experimentally induced necrotic enteritis in broiler chickens. International Journal of Pharmacology and Toxicology 5 (1), 51-56. & doi:10.14419/ijpt.v5i1.7193

[21] El-Bahr SM, Ismail IB & Al-Busadah KA (2013). Oxidative stress biomarkers and biochemical profile in broilers chicken fed zinc bacitracin and ascorbic acid under hot climate. American Journal of Biochemistry and Molecular Biology 3, 202-214.

[22] Faden H, Hong JJ & Ogra PL (1985). In-vivo effects of clindamycin on neutrophil function. The Journal of antimicrobial chemotherapy 16, 649-657.

[23] Fateeva LI (1983). [Lincomycin binding by serum proteins and tissues], Antibiotiki 28, 453-456.

[24] Frings CS & Dunn RT (1970). A colorimetric method for determination of total serum lipids based on the sulfo-phospho-vanillin reaction. American Journal of Clinical Pathology, 53 (1), 89-91.

[25] Gilmour JT, Koehler MA, Cabrera ML, Szajdak L & Moore PA Jr (2004). Alum treatment of poultry litter: decomposition and nitrogen dynamics. Journal of environmental quality 33, 402-405.

[26] Greenwood D (2010). Lincosamines. In: Finch R, Greenwood D, Norrby S & Whitley R (eds) Antibiotic and Chemotherapy: Anti-infective Agents and Their Use in Therapy. Saunders Elsevier, Edinburgh, pp 272–275.

[27] Henry RJ, Winkelman JW & Cannon DC (1974), Clinical Chemistry: Principles and Techniques. 2nd edn. Happer and Row Publishers, New York,

[28] Huyghebaert G, Ducatelle R & Van Immerseel F (2011). An update on alternatives to antimicrobial growth promoters for broilers. The Veterinary Journal 187 (2), 182-188.

[29] Ibrahim AEH (2005). Effect of Dietary Zinc Bacitracin on Broiler Chicks Performance and Physiological Responses Under Summer Conditions. Master Thesis, Department of Physiology, Faculty of Veterinary Medicine, University of Khartoum, Khartoum, Sudan.

[30] Ismail IB, Al- Busadah KA & El- Bahr SM (2015). Biochemical markers of oxidative stress in tissues of broiler chickens fed zinc bacitracin and ascorbic acid under hot climate. International Journal of Biological Chemistry 9, 38-45.

[31] Jiang N, Yang WR, Gao QX, Zhang CY, Liu FX & Sun XP (2013), Influences of antibiotics on the growth performance and intestinal immunity of Lingnan yellow broilers. Acta Agriculturae Universitatis Jiangx 35 (4), 819-825.

[32] Kadry MB, Kalil HM, Shawky NA & Salah A (2009). Evaluation of lincomycin as a therapy of the Clostridium perfringens infection in chickens. Benha Veterinary Medical Journal 20, 1-10.

[33] Khan A & Nagra SS (2010). Performance of broiler chicks as influenced by feeding diets supplemented with organic acids. Indian Journal of Poultry Science 45, 30-34.

[34] Lee LF & Bacon LD (1983). Ontogeny and line differences in the mitogenic response of chicken lymphocytes. Poultry science 62, 579-584.

[35] Li L, Xu CL, Ji C, Ma Q, Hao K, Jin ZY& Li K (2006). Effects of a dried Bacillus subtilis culture on egg quality. Poultry science 85, 364-368.

[36] Lu J, Hofacre C, Smith F & Lee MD (2008). Effects of feed additives on the development on the ileal bacterial community of the broiler chicken. Animal: an international journal of animal bioscience 2, 669-676.

[37] Manafi M, Khalaji S, Hedayati M & Pirany N (2017). Efficacy of Bacillus subtilis and bacitracin methylene disalicylate on growth performance, digestibility, blood metabolites, immunity, and intestinal microbiota after intramuscular inoculation with Escherichia coli in broilers. Poultry science 96, 1174-1183.

[38] Mohamed AGA (2016). Concurrent Uses of Diclazuril and Lincomycin for Controlling of Severe Necrotic Enteritis in Broiler Chicks. Master Thesis, Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazug, Egypt.

[39] Mohamed FAM (1995). Evaluation of Possible Concurrent Use of Both Lincomycin and Spectinomycin in Chickens. Master Thesis, Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.

[40] Naveenkumar S, Karthikeyan N, Narendra Babu R, Veeramani P, Sivarama Krishnani S & Srinivasan G (2018). Effect of calcium propionate and coated sodium butyrate as an alternative to antibiotic growth promoters on the serum profile of commercial broiler chicken. International Journal of Chemical Studies 6 (1), 36-39.

[41] Nielsen F, Mikkelsen BB, Nielsen JB, Andersen HR & Grandjean P (1997). Plasma malondialdehyde as biomarker for oxidative stress: reference interval and effects of life-style factors. Clinical chemistry 43: 1209-1214.

[42] Nishikimi M, Appaji N & Yagi K (1972). The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochemical and Biophysical Research Communications 46, 849-854.

[43] Ogboko B (2010). The effect of zinc bacitracin on the physiology of broilers fed plant and animal protein diets. Lucrari stiintifice Seria Zootehnie-Universitatea de Stiinte Agricole si Medicina Veterinara Ion Ionescu de la Brad (Romania).

[44] Pan L, Zhao PF, Ma XK, Shang QH, Xu YT, Long SF, Wu Y, Yuan FM & Piao XS. (2017). Probiotic supplementation protects weaned pigs against enterotoxigenic Escherichia coli K88 challenge and improves performance similar to antibiotics. Journal of animal science 95 (6), 2627-2639.

[45] Porter RE Jr (1998) Bacterial enteritides of poultry. Poultry Science 77, 1159-1165.

[46] Petrie A & Watson P (2013). Statistics for Veterinary and Animal Science. 3rd edn. Wiley-Blackwell (John Wiley & Sons, Ltd), United Kingdom.

[47] Ramadan S (2017). Behavior, fear response, performance and immune response of broiler chicks fed graded levels of anise seed as alternative to antibiotics. Alexandria Journal of Veterinary Sciences 55, 74-82.

[48] Rukmini M, Sahoo D, Dalei J & Ray R (2015). Production, purification and characterization of bacitracin from Bacillus subtilis. The Pharma Innovation Journal 3, 77-82.

[49] Sims MD, Dawson KA, Newman KE, Spring P & Hoogell DM (2004). Effects of dietary mannan oligosaccharide, bacitracin methylene disalicylate, or both on the live performance and intestinal microbiology of turkeys. Poultry science 83, 1148-1154.

[50] Sinha AK (1972). Colorimetric assay of catalase. Analytical biochemistry 47, 389-394.

[51] Stutz MW & Lawton GC (1984). Effects of diet and antimicrobials on growth, feed efficiency, intestinal Clostridium perfringens, and ileal weight of broiler chicks. Poultry science 63, 2036-2042.

[52] Sun X, McElroy A, Webb KE, Jr., Sefton AE & Novak C (2005). Broiler performance and intestinal alterations when fed drug-free diets. Poultry science 84,1294-1302.

[53] Teo AY & Tan HM (2007). Evaluation of the performance and intestinal gut microflora of broilers fed on corn-Soy diets supplemented with Bacillus subtilis PB6 (CloSTAT). The Journal of Applied Poultry Research 16, 296-303.

[54] Thompson RA (1977). Technique in Clinical Immunology. Blackwell Scientific Publications, Osney Mead, Oxford OX2 0EL.

[55] Torok VA, Allison GE, Percy NJ, Ophel-Keller K & Hughes RJ (2011). Influence of antimicrobial feed additives on broiler commensal posthatch gut microbiota development and performance. Applied and environmental microbiology 77, 3380-3390.

[56] Weiss DJ & Wardrop KJ (2010). Schalm's Veterinary Hematology. 6th edn. Blackwell Publishing Ltd, Philadelphia, U.S.A.

[57] White WL, Erickson MM & Stevens SC (1970). Determination of cholesterol. In: White WL, Erickson MM, Stevens SC (eds) Chemistry for Medical Technologists. 3rd edn. Saint Louis: C. V. Mosby Co., Saint Louis, USA,

[58] Wu DW, Wang LC, Wen C, Hooge DM, Liang C & Zhou YM (2013). Effects of replacing a dietary antibacterial agent (zinc bacitracin) with copper salts in Cherry Valley Pekin meat ducks. British poultry science 54, 112-119.

[59] Zhou M, Wang E, Wang B, Chen Z, Zou Y, Shen S, He B & Ding H (2016). Substitution of cinnamyl aldehyde for aureomycin and bacitracin zinc in nursery pig diet. Chinese Journal of Animal Nutrition 28 (7), 2106-2112. Doi:10.3969/j.issn.1006-267x.2016.07.015

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