Can rutin ameliorate aluminum phosphide-induced acute cardiac toxicity in adult albino rats?

 
 
 
  • Abstract
  • Keywords
  • References
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  • Abstract


    Current management of rice tablet or aluminium phosphide (AlP) poisoning has remained mostly supportive despite of its fatal outcome and unfortunately no antidote is found yet, therefore, there is a need to search for a treatment that can adequately protect against its toxicity. To the best of our knowledge, there are no studies until now concerning the cardioprotective effect of rutin against AlP cardiac toxicity in rats. For this purpose, this study was carried out to investigate the possible protective effect of rutin against AlP induced cardiotoxicity in rats. Forty male albino rats were randomly divided into four groups. Group I: normal control group was served as untreated rats and received distilled water orally through a gastric tube. Group II: Rutin treated group received a dose of 100 mg/kg rutin dissolved in distilled water and given orally through a gastric tube. Group III: AlP intoxicated rats received AlP oral single sub-lethal dose (2 mg/Kg body weight) dissolved in distilled water and given through a gastric tube. Group IV: AlP intoxicated rats + Rutin treated one hour after receiving AlP in doses as mentioned above. After that we tested the following parameters: ECG changes including HR and ST-segment elevation, serum level of TNF-α, IL-6 and H-FABP (pg/ml), antioxidant and Oxidant parameters in cardiac tissue as GSH, SOD, and MDA, apoptotic factor caspase-3 and histopathological examination of cardiac tissue was also included. The results showed that treatment with rutin caused a significant decrease in heart rate and ST segment elevation, a significant decrease in activity of TNF-α and IL-6 and levels of H-FABP also a significant decrease in the activity of SOD with decreased levels of MDA and caspase-3 level and a significant increase in the level of GSH compared to (AlP) intoxicated group, also histopathological changes induced by AlP improved after treatment with rutin. It is concluded that AlP intoxication caused ECG, biochemical and histopathological changes which were potentially improved with rutin.

     

     


  • Keywords


    Acute; Aluminum Phosphide (Alp); Cardiac Toxicity; Rats; Rutin.

  • References


      [1] Afolabi O.K., Wusu A.D., Ugbaja R. and Fatoki J.O. (2018). Aluminium phosphide-induced testicular toxicity through oxidative stress in Wistar rats: Ameliorative role of hesperidin. Toxicology Research and Application, (January 2018). https://doi.org/10.1177/2397847318812794.

      [2] Ahmadi J., Joukar S., Anani H., and Karami-Mohajeri S. (2018). Dihydroxyacetone as a definitive treatment for aluminium phosphide poisoning in rats, Archives of Industrial Hygiene and Toxicology, 69(2), 169-177. https://doi.org/10.2478/aiht-2018-69-3106.

      [3] Ahmed S. Gouda, Nagla A. El-Nabarawy, Samah F. Ibrahim (2018). Moringa oleifera extract (Lam) attenuates Aluminium phosphide-induced acute cardiac toxicity in rats. Toxicology Reports, 5, 209-212. https://doi.org/10.1016/j.toxrep.2018.01.001.

      [4] Ali F. Abdel-Wahab and Wahid M. Al-Harizy (2013). Propofol Protects against Ischemia/Reperfusion Injury Associated with Reduced Apoptosis in Rat Liver. ISRN Anesthesiology. Article ID 517478, 8 pages. https://doi.org/10.1155/2013/517478.

      [5] Aruna R, Geetha A and Suguna P. (2013). Expression of caspase activation recruitment and pyrin domain levels of apoptosis-associated speck-like protein complex in the pancreas of rats subjected to experimental pancreatitis: Influence of rutin administration. Hum ExpToxicol; 33:940-948. https://doi.org/10.1177/0960327113512337.

      [6] Baghaei A, Hajimohammadi N, Baeeri M, Mohammadirad A, Hassani S, Abdollahi M. (2014). On the protection of ALP cardiovascular toxicity by a Novel mixed herbal medicine; Role of oxidative stress and cellular ATP. Asian J Anim Vet Adv; 9:302-311. https://doi.org/10.3923/ajava.2014.302.311.

      [7] Bhandary B, Piao CS, Kim DS, Lee GH, Chae SW, Kim HR and Chae HJ (2012). The protective effect of rutin against ischemia/reperfusion-associated hemodynamic alteration through antioxidant activity. Arch Pharm Res; 35:1091-1097. https://doi.org/10.1007/s12272-012-0617-6.

      [8] Bradford M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding,” Analytical Biochemistry; 72: 248–254. https://doi.org/10.1016/0003-2697(76)90527-3.

      [9] Browne RW, Armstrong D (1998). Reduced glutathione and glutathione disulfide. Free Radical and Antioxidant Protocols. Humana Press.:347-352. https://doi.org/10.1385/0-89603-472-0:347.

      [10] Esterbauer H., Cheeseman K.H. (1990). Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Meth Enzymol.186:407–421. https://doi.org/10.1016/0076-6879(90)86134-H.

      [11] Gelen V, Şengül E, GedikliS, Atila G, Uslu H and Makav M (2017). The protective effect of rutin and quercetin on 5-FU-induced hepatotoxicity in rats. Asian Pacific Journal of Tropical Biomedicine; 7, (7): 647-653. https://doi.org/10.1016/j.apjtb.2017.06.013.

      [12] Herrmann AP and Janke HD (2001). Cofermentation of rutin and hesperidin during two-stage anaerobic pre-treatment of high-loaded brewery wastewater. Water Res.; 35(11):2583-2588. https://doi.org/10.1016/S0043-1354(00)00575-3.

      [13] Hochman J.S.; Choo H. and Hirsh L.E. (1987). Limitation of myocardial infarct expansion by reperfusion independent of myocardial salvage. Ciculation, 20: 313 – 323.

      [14] Hsieh SR, Cheng WC, Su YM, Chiu CH and Liou YM (2014). Molecular targets for anti-oxidative protection of green tea polyphenols against myocardial ischemic injury. BioMedicine; 4(4): 7-16. https://doi.org/10.7603/s40681-014-0023-0.

      [15] Imam F, Al-Harbi NO, Al-Harbia MM, Korashy HM, Ansari MA, Sayed-Ahmed MM, et al. (2017). Rutin attenuates carfilzomib-induced cardiotoxicity through inhibition of NF-κB, hypertrophic gene expression and oxidative stress. Cardiovasc Toxicol; 17:58–66. https://doi.org/10.1007/s12012-015-9356-5.

      [16] Kolchin et al. (1991). The cardioprotective action of quercetin in experimental occlusion and reperfusion of the coronary artery in dogs. Farmakol Toksikol; 54: 20–23.

      [17] Korkmaz A and Kolankaya D (2010). Protective effect of rutin on the ischemia/reperfusion induced damage in rat kidney; J Surg Res; 164(2): 309-315. https://doi.org/10.1016/j.jss.2009.03.022.

      [18] López-Revuelta A, Sánchez-Gallego JI, Hernández-Hernández A, Sánchez-Yagüe J, Llanillo M (2006). Membrane cholesterol contents influence the protective effects of quercetin and rutin in erythrocytes damaged by oxidative stress. Chem Biol Interact.; 15; 161(1):79-91. https://doi.org/10.1016/j.cbi.2006.03.004.

      [19] Lv L, Yao Y, Zhao G and Zhu G (2018).Rutin inhibits coronary heart disease through ERK1/2 and Akt signaling in a porcine model. ExpTher Med; 15(1):506-512. https://doi.org/10.3892/etm.2017.5365.

      [20] M. Hashemzaei, A. Barani, M. Iranshahi, R. Rezaee, K. Tsarouhas, A. Tsatsakis, et al (2016). Effects of resveratrol on carbon monoxide-induced cardiotoxicity in rats Environ. Toxicol. Pharmacol. 46, 110-115. https://doi.org/10.1016/j.etap.2016.07.010.

      [21] Ma Y, Yang L, Ma J, Lu L, Wang X, Ren J, et al. (2017). Rutin attenuates doxorubicin-induced cardiotoxicity via regulating autophagy and apoptosis. Biochim Biophys Acta.; 1863:1904–1911. https://doi.org/10.1016/j.bbadis.2016.12.021.

      [22] Merone M, Soda P, Sansone M, Sansone C. (2017). ECG databases for biometric systems: A systematic review. Expert Syst Appl; 67:189-202. https://doi.org/10.1016/j.eswa.2016.09.030.

      [23] Mokhtar I. Yousef, Naglaa F. Soliman, Fatma M. El-Demerdash (2015). Aluminium Phosphide-induced Hepato-nephrotoxicity and Oxidative Damage in Rats: The Protective Effect of α-lipoic Acid. The Open Conference Proceedings Journal, 6, 18-23. https://doi.org/10.2174/2210289201506010018.

      [24] Mostafa Akkaoui, Sanae Achour, Khalid Abidi, Btissam Himdi, Aoupe Madani, Amine Ali Zeggwagh and Redouane Abouqal (2007). Reversible myocardial injury associated with aluminum phosphide poisoning. Clinical Toxicology; 45 (6): 728-731. https://doi.org/10.1080/15563650701517350.

      [25] Nègre-Slvayre A, Affany A and Hariton CR (1991). Additional antilipoperoxidant activities of alpha-tocopherol and ascorbic acid on membrane-like systems are potentiated by rutin. Pharmacology; 42:262–272. https://doi.org/10.1159/000138807.

      [26] Nirmala, C. and Puvanakrishnan, R. (1994). Isoproterenol Induced myocardial infarction in rats: functional and biochemical alteration. Med. Sci. Res., 22:575-577.

      [27] Paget, G. and Barnes, J. M. (1964). Evaluation of drug activities, pharmacometric; vol. 1, Academic Press, London- New York.

      [28] Parasuraman S., Raveendran R., Kesavan R. (2010). Blood sample collection in small laboratory animals. J Pharmacol. 1: 87–93. https://doi.org/10.4103/0976-500X.72350.

      [29] R. Anand, B.K. Binukumar, K.D. Gill (2011). Aluminum phosphide poisoning: an unsolved riddle; J. Appl. Toxicol., 31 (6), 499-505. https://doi.org/10.1002/jat.1692.

      [30] Raina Dua and Kiran D.Gill (2004). Effect of aluminium phosphide exposure on kinetic properties of cytochrome oxidase and mitochondrial energy metabolism in rat brain. Biochimica et Biophysica Acta (BBA) - General Subjects, 1674 (1), 4-11. https://doi.org/10.1016/j.bbagen.2004.05.003.

      [31] Robak J and Gryglewski RJ (1988). Flavonoids are scavengers of superoxide anions. BiochemPharmacol; 37:837–841. https://doi.org/10.1016/0006-2952(88)90169-4.

      [32] S. Sheweita, B. Salama, M. Hassan (2015). Erectile dysfunction drugs and oxidative stress in the liver of male rats. Toxicol. Rep., 2:933-938. https://doi.org/10.1016/j.toxrep.2015.06.002.

      [33] Shah V, Baxi S, Vyas T. (2009). Severe myocardial depression in a patient with aluminium phosphide poisoning: a clinical, electrocardiographical and histopathological correlation. Indian J Crit Care Med; 13(1):41–43. https://doi.org/10.4103/0972-5229.53117.

      [34] Singh Y., Joshi SC, Satyawali V., Gupta A. (2014). Acute aluminium phosphide poisoning, what is new? Egypt J Intern Med; 26(3):99-103. https://doi.org/10.4103/1110-7782.145298.

      [35] Soltaninejad K., Beyranvand MR, Momenzadeh SA, Shadnia S. (2012). Electrocardiographic findings and cardiac manifestations in acute aluminum phosphide poisoning. J Forensic Leg Med; 19:291-293. https://doi.org/10.1016/j.jflm.2012.02.005.

      [36] Sun Y, Oberley L.W., Li Y. (1988). A simple method for clinical assay of superoxide dismutase. Clin Chem; 34:497–500. https://doi.org/10.1093/clinchem/34.3.497.

      [37] Topal I., Bilgin A.O., Çimen F. K., Kurt N., Süleyman Z., Bilgin Y., Özçiçek A. and Altuner D.(2018). The effect of rutin on cisplatin-induced oxidative cardiac damage in rats. Anatol J Cardiol.; 20(3): 136–142. https://doi.org/10.14744/AnatolJCardiol.2018.32708.

      [38] Umarani V, Muvvala S, Ramesh A, Lakshmi BV and Sravanthi N (2015). Rutin potentially attenuates fluoride-induced oxidative stress-mediated cardiotoxicity, blood toxicity and dyslipidemia in rats. Toxicol Mech. Methods; 25:143–149. https://doi.org/10.3109/15376516.2014.1003359.

      [39] Wu F, Chen J, Fan LM, Liu K, Zhang N, Li SW, et al.(2017). Analysis of the effect of rutin on GSK-3βand TNF-αexpression in lung cancer. Exp Ther Med.; 14:127–130. https://doi.org/10.3892/etm.2017.4494.

      [40] YE X., HE Y., WANG S, WONG G.T., IRWIN M.G and XIA Z. (2018). Heart-type fatty acid binding protein (H-FABP) as a biomarker for acute myocardial injury and long-term post-ischemic prognosis. Acta Pharmacologica Sinica; 39:1155-1163. https://doi.org/10.1038/aps.2018.37.


 

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Article ID: 29973
 
DOI: 10.14419/ijpt.v8i1.29973




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