Protective effects of prebiotic (resistant maltodextrin) and silymarin against toxicity of carbon tetrachloride in liver rat and kidney

 
 
 
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    Exposure to carbon tetrachloride induces acute and chronic hepatic injuries as well as renal injuries in rats. Therefore, the current study aimed to evaluate the protective role of prebiotic (digestion resistant maltodextrin) and silymarin against carbon tetrachloride -induced heptorenal toxicity in albino rats. Six groups with ten rats each were used for this purpose; these groups included the control vehicle group that received saline daily for 30 days, prebiotic group (1g/kg, orally) daily for 30 days; silymarin group (200 mg/kg orally) daily for 30 days; carbon tetrachloride group (2.5ml/kg intraperitoneally twice per week for three week; the prebiotic – carbon tetrachloride group; the silymarin – carbon tetrachloride group. The results revealed that carbon tetrachloride significantly increased serum levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin cholesterol, triglyceride, urea and creatinine. In addition, there were substantial increase in lipid peroxidation (malondialdehyde) and level of glucose with significant decreases in albumin, total protein, creatinine kinase, hemoglobin and red blood cells. Carbon tetrachloride also caused histological changes in liver and kidney tissues. However, administration of prebiotic and silymarin alone ameliorated the carbon tetrachloride induced liver and kidney damage with improved hematological, lipid profile and glucose level.

     

     



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      [1] Adeneye AA, Awodele O, Aiyeola SA and Benebo AS. (2015): Modulatory potentials of the aqueous stem bark extract of Mangifera indica on carbon tetrachloride-induced hepatotoxicity in rats. Journal of Traditional and Complementary Medicine.5:106–115

      [2] Agatemor UM, Nwodo OF.C, Adejoh IP and Anosike CA. (2018): Protective effect of cucumis sativus on carbon tetrachloride CCl4-induced liver damage in rats. Asian Journal of Biochemistry, 13: 22-29.

      [3] Alatriste PM, Arronte RU, Espinosa CG, and Cuevas MDL (2014): Effect of probiotics on human blood urea levels in patients with chronic renal failure. Nutr Hosp.; 29(3):582-590.

      [4] Alcaraz-Contreras Y, Mendoza-Lozano RP, Martínez-Alcaraz ER, Martínez-Alfaro M, Gallegos-Corona MA, Ramírez-Morales MA and Vázquez-Guevara M.A.(2016): Silymarin and dimercaptosuccinic acid ameliorate lead-induced nephrotoxicity and genotoxicity in rats.Human & Experimental Toxicology Apr; 35(4):398-403.

      [5] Aliasgharzadeh A, Dehghan P, Pourghassem-Gargari B. and Asghari-Jafarabadi M. (2015): Resistant dextrin, as a prebiotic, improves insulin resistance and inflammation in women with type 2 diabetes: a randomised controlled clinical trial. Br J Nutr; 113(02):321–30.

      [6] Awodele O, Adeneye AA, Aiyeola SA, Benebo AS. (2015): Modulatory effect of Mangifera indica against carbon tetrachloride induced kidney damage in rats. Interdiscip Toxicol. Dec; 8(4): 175–183.

      [7] Banchroft, J.D.; Stevens, A. and Turner, D.R. (1996): Theory and practice of histological techniques Fourth ED. Churchil Livingstone ,New York , London , San Francisco , Tokyo.

      [8] Batool R, Khan M.R and Majid M. (2017): Euphorbia dracunculoides L. abrogates carbon tetrachloride induced liver and DNA damage in rats. BMC Complement Altern Med. Apr 20; 17(1):223.

      [9] Behrouz V, Jazayeri S, Aryaeian N, Zahedi M.J and Hosseini F.(2017): Effects of Probiotic and Prebiotic Supplementation on Leptin, Adiponectin, and Glycemic Parameters in Non-alcoholic Fatty Liver Disease: A Randomized Clinical Trial. Middle East J Dig Dis. Jul; 9(3):150-157.

      [10] Bouhalit S, Kechrid Z. and Elfeki A. (2017): The effect of silymarin extract from Silybum marianum against nickel-induced hematotoxicity and nephrotoxicity an male albino Wistal rats.International Journal of pharmacy and pharmaceutical sciencesVol: 9.issue 8:84-89.

      [11] Carla M. Brites Maria J. (2011): Mazie and resistant starch enriched breads reduce postprandandial glycemic responses in rats. Volume 31, Issue 4, Pages 302-308

      [12] Cecen, E.; Dost, T.; Culhaci, N.; Karul, A.; Ergur, B. and Birincioglu, M. (2011): Protective effects of silymarin against doxorubicin-induced toxicity. Asian Pac J Cancer Prev. 12(10):2697-704.

      [13] Cetinkaya, A; Kantarceken, B; Bulbuloglu, E; Kurutas, E.B; Ciralik, H and Atli, Y.(2013): The effects of L-carnitine and N-acetylcysteine on carbontetrachloride induced acute liver damage in rats. Bratisl Lek Listy.; 114(12):682-8.

      [14] Comelli MC, Mengs U, Schneider C, Prosdocimi M. (2007) toward the definition of the mechanism of action of silymarin: Activities related to cellular protection from toxic damage induced by chemotherapy. Integr Cancer Ther; 6:20–9.

      [15] Daubioul C.A, Horsmans Y, Lambert P,Danse E. ,Delzenne N.M.(2005): Effects of oligofructose on glucose and lipid metabolism in patients with nonalcoholic steatohepatitis: results of a pilot study. Eur J Clin Nutr. May; 59(5):723-6.

      [16] de Avelar C.R, Pereira E.M, de Farias Costa P.R, de Jesus R.P, de Oliveira L.P.M.(2017): Effect of silymarin on biochemical indicators in patients with liver disease: Systematic review with meta-analysis. World J Gastroenterol. Jul 21; 23(27):5004-5017.

      [17] Delzenne N. and Reid G. (2009): No causal link between obesity and probiotics. Nat Rev Microbiol 7: 901.

      [18] Deng J.S, Chang Y.C. Wen C.L, Liao J.C, Hou W.C, Amagaya S, Huang S.S and Huang G.J.(2012): Hepatoprotective effect of the ethanol extract of Vitis thunbergii on carbon tetrachloride-induced acute hepatotoxicity in rats through anti-oxidative activities. Journal Ethnopharmacol. Aug 1; 142(3):795-803.

      [19] Duncan D.B. (1955): Multiple range and multiple Ftests. Biometrics 11:1 42.

      [20] El Bialy, B.E; El-Boraey, N.G; Hamouda, R.A and Abdel- Daim,M.M.(2019): Comparative protective effects of Spirulina and Spirulina supplemented with thiamine against sub-acute carbon tetrachloride toxicity in rats. Biomedical & Pharmacology Journal, June Vol. 12(2), p. 511-525.

      [21] El-Mahmoudy A.M, Abdel-Fattah FA, Abd El-Mageid AD, and Gheith IM. (2014): Effect of the growth promotant Mannan-Oligosaccharide on the lipogram and organfunction profile in hyperlipidemic Albino rat. Rats American Journal of Phytomedicine and Clinical TherapeuticsISSN 2321 – 2748.

      [22] El-Shitany N.A, El-Haggar S, and El-desoky K.(2008): Silymarin prevents adriamycin-induced cardiotoxicity and nephrotoxicity in rats. Food Chem Toxicol. Jul;46(7):2422-8.

      [23] Englyst HN, Kingman SM, and Cummings JH. (1992): Classification and measurement of nutritionally important starch fractions. European Journal of clinical nutrition 46(2):33-55.

      [24] Famouri F, Salehi MM, Rostampour N, Hashemi E, and AShahsanaee A.(2017): The effect of silymarin on non-alcoholic fatty liver disease of children.J Herbmed Pharmacol. ;6(1):16-20.

      [25] Farhangi MA, Javid AZ, and Dehghan P. (2016): The effect of enriched chicory inulin on liver enzymes, calcium homeostasis and hematological parameters in patients with type 2 diabetes mellitus: A randomized placebo-controlled trial.Prim Care Diabetes. Aug; 10(4):265-71.

      [26] Figurska-Ciura D, Orzeł D, Styczyńska M and Wacław L. (2007): The influence of RS4 resistant starch on wistar rats metabolism. Biochemical and lipid indices. Roczniki Państwowego Zakładu Higieny 58(1):1-6

      [27] Fraschini F, Demartini G, and Esposti D. (2002): Pharmacology of silymarin. Clinical Drug Investigation 22(1), 51-65.

      [28] Freitag AF, Cardia GF, da Rocha BA, Aguiar RP, Silva-Comar FM, Spironello RA, Grespan R, Caparroz-Assef SM, Bersani-Amado CA and Cuman, RK. (2015): Hepatoprotective Effect of Silymarin (Silybum marianum) on Hepatotoxicity Induced by Acetaminophen in Spontaneously Hypertensive Rats. Evid Based Complement Alternat Med.; 2015:538317.

      [29] Fujii T. (1997): Toxicological correlation between changes in blood biochemical parameters and liver histopathological findings. Journal of Toxicological Science; 22: 161-83.

      [30] Gargari BP, Namazi N, Khalili M, Sarmadi B, Jafarabadi MA and Dehghan P.(2015): Is there any place for resistant starch, as alimentary prebiotic, for patients with type 2 diabetes? Complement Ther Med. Dec; 23(6):810-5.

      [31] Gibson GR and Roberfroid MB. (1995): Dietary modulation of the colonic microbiota: introducing the concept of prebiotics. Journal of Nutrition 125, 1401-1412.

      [32] Hahn G, Lehmann HH, Kurten M, Uebe H and Vogel G.(1968): On the pharmacology and toxicology of silymarin,an antihepatotoxic active principlefromSilybummarianum(L.)Gaertn.Arzneimitteloforschung.18:698-704.

      [33] Haghi MEs, Dehghan G, Banihabib N, Zare S, Mikaili P and Panahi F.(2014): Protective effects of Cornus mas fruit extract on carbon tetrachloride induced nephrotoxicity in rats. Indian J Nephrol. Sep-Oct; 24(5): 291–296.

      [34] Hashizume C, Kishimoto Y, Kanahori S, Yamamoto T,Okuma K. and Yamamoto K.(2012): Improvement effect of resistant maltodextrin in humans with metabolic syndrome by continuous administration. J Nutr Sci Vitaminol (Tokyo); 58(6):423-30.

      [35] Heidarian E and Rafieian-Kopaei M. (2012): Effect of silymarin on liver phoshpatidate phosphohydrolase in hyperlipidemic rats. Bioscience Research, 9(2): 59-67,

      [36] Jarosz PA, Fletcher E, Elserafy E, Artiss JD and Jen KL.(2013): The effect of α-cyclodextrin on postprandial lipid and glycemic responses to a fat-containing meal. Metabolism. Oct; 62(10):1443-7.

      [37] Kamal S. (2015): The protective effect of curcumin on nephrotoxicity induced by carbon tetrachloride in rats. Journal of advances in life and Natural sciences, Vol. 1 (1), 22-29,

      [38] Kaneko JJ. Clinical biochemistry of domestic animals. 5th ed. San Diego (CA): Academic Press, 1997.

      [39] Kim SH, Oh, DS, Oh JY, Son TG, Yuk DY and Jung YS.(2016): Silymarin prevents restraint stress-induced acute liver injury by ameliorating oxidative stress and reducing inflammatory response. Molecules. Apr 1; 21(4):443.

      [40] Kishimoto Y, Oga H, Tagami H, Okuma K and Gordon DT.(2007): Suppressive effect of resistant maltodextrin on postprandial blood triacylglycerol elevation. Eur J Nutr. Apr; 46(3):133-8.

      [41] Kishimoto Y, Hayashi N, Yamada T, Yuba K and Yamamoto K. (2009): Favorable effect of resistant maltodextrin on postprandial blood Glucose, insulin and triglyceride levels. Jpn Pharmacol Ther, 37, 277-283.

      [42] Khan MR, Rizvi W, Khan GN, Khan RA, Shaheen S. (2009): Carbon tetrachloride induced nephrotoxicity in rats: protective role of Digera muricata. J Ethnopharmacol; 122:91–99.

      [43] Koh GY(2015): Resistant starch:implication in kidney health and vitamin D homeostasis in diabetes mellitus

      [44] Koguchi T, Koguchi H, Nakajima H, Takano S, Yamamoto Y, Innami S, Maekawa A and Tadokoro T .(2004):Dietary fiber suppresses elevation of uric acid and urea nitrogen concentrations in serum of rats with renal dysfunction induced by dietary adenineInt J Vitam Nutr Res.74(4):253-63.

      [45] KrenV and Walterova D. (2005): Silybin and silymarin- new effects and applications. Biomed Papers, 149(1):29-41.

      [46] Lamfon H. (2007): Effect of silymarin against deltamethrin-induced histological and biochemical changes in liver of albino rats. Indian journal of experimental biology 3:165-169.

      [47] Laouar A, Fahima K, Bourogaa E, and Amel Benamara A.(2017): Potential antioxidant properties and hepatoprotective effects of Juniperus phoenicea berries against CCl 4 induced hepatic damage in rats. Asian Pacific Journal of Tropical Medicine 10(3) ·

      [48] Liu SH, Chiu C, Huang L, Chiang M.(2019): Resistant maltodextrin ameliorates altered hepatic lipid homeostasis via activation of AMP-activated protein kinase in a high-fat diet-fed rat model. Nutrients 29; 11(2).

      [49] Mansour HH, Hafez HF, and Fahmy NM (2006): Silymarin modulates Cisplatin-induced oxidative stress and hepatotoxicity in rats. J Biochem Mol Biol. Nov 30; 39(6):656-61.

      [50] Matsubara T, Mori S, Touchi A, Masuda Y, Takeuchi Y. Carbon tetrachloride-induced hepatotoxicity in rats: evidence for different susceptibilities of rat liver lobes. Jpn J Pharmacol. 1983 Apr; 33(2):435-45.

      [51] Mazani M, Mahmoodzadeh Y, Chinifroush-Asl MM, Banaei S, Rezagholizadeh L , Mohammadnia A. (2018): Renoprotective effects of the methanolic extract of Tanacetum parthenium against carbon tetrachloride-induced renal injury in rats. Avicenna Journal Phytomedicien, Vol. 8, No. 4, Jul-Aug.

      [52] McKenzie EC, Stephanie JV, Sandra MG and Joe DP.(2008): Effects of dietary starch,fat, and bicarbonate content on exercise responses and serum creatine kinase activity in equine recurrent exertional rhabdomyolysis. Journal of veterinary internal medicine. Volume 17 issue 5.

      [53] Momeni A, Hajigholami A, Geshnizjani S and Kheiri S.(2015): Effect of silymarin in the prevention of cisplatin nephrotoxicity, a clinical trial study. J Clin Diagn Res. Apr; 9(4): OC11–OC13.

      [54] Narayanamurthy U, Santhakumari AS and Nirmala P. (2014): Nephroprotective effect of silymarin in hyperglycemia-induced oxidative stress in rats. Int J Basic Clin Pharmacol. 3(6): 1030-1035.

      [55] Nassuato Rm, Iemmolo M, Strazzabosco F, Lirussi R, Deana Ma, Francesconi MM, Passe D. Effect of Silibinin on biliary lipid composition ... Volume 12, Issue 3, May 1991, Pages 290-295.

      [56] Nie Y, Ren D, Lu X, Sun Y, and Yang X. (2015): Differential protective effects of polyphenol extracts from apple peels and fleshes against acute CCl₄-induced liver damage in mice. Food Funct. Feb; 6(2):513-24.

      [57] Oda SS and El-Ashmawy IM (2012): Protective effect of silymarin on mercury-induced acute nephro-hepatotoxicity in rats. Global Veterinaria 9 (4): 376-383, 2012.ISSN 1992-6197.

      [58] OkauT, and Nakamura S. (2014): Evaluation of the relative available energy of several dietary fiber preparation using breath hydrogen evolution in healthy humans. J Nutr Sci Vitaminol, 60:246-254.

      [59] Otrubová O, Turecký L, Uličná O, Janega P, Luha J, and Muchová J.(2018): Therapeutic effects of N-acetyl-L-cysteine on liver damage induced by long-term CCl4 administration. Gen Physiol Biophys. Jan; 37(1):23-31.

      [60] Ozturk M, Akdogan M;,Keskin I, Kisioglu A.N;,Oztas S and Yildiz K. (2012): Effect of silybum marianum on acute hepatic damage caused by carbon tetrachloride in rats.Biomedical research Volume23, Issue 2

      [61] Pacheco GS, Panatto JP (2009): Brain creatine kinase activity is inhibited after hepatic failure induced by carbon tetrachloride or acetaminophen .Metabolic Brain disease 24(3):383-94.

      [62] Papackova Z, Heczkova M, Dankova H, Sticova E, Lodererova A, Bartonova L, Poruba M and Cahova M.(2018): Silymarin prevents acetaminophen-induced hepatotoxicity in mice. PLoS One. Jan 17;13 (1):e0191353.

      [63] Pereira DI and Gibson GR (2002): Effects of consumption of probiotics and prebiotics on serum lipid levels in humans. Crit Rev Biochem Mol Biol.; 37(4):259-81.

      [64] Préstamo G Pedrazuela A, Elena P, Lasunción M A, Arroyo G. (2003):Role of buckwheat diet on rats as prebiotic and healthy food. Nutrition Research Volume 23, Issue 6, Pages 803-814.

      [65] Ribeiro WP, Stephanie JV, Pagan JD and Essen B .(2004): The effect of varying dietary starch and fat content on serum creatine kinase activity and substrate availability in equine polysaccharide storage myopathy. Journal of veterinary internal medicine 18(6),887-894.

      [66] Rishi P, Kaur Mavi S,Bharrhan S, Shukla G and Tewari R. (2009): Protective efficacy of probiotic alone or in conjunction with a prebiotic in Salmonella-induced liver damage. FEMS Microbiology Ecology, Volume 69, Issue 2, Pages 222–230,

      [67] Roberfroid MB. (2007): Prebotics:the concept revisited.J.Nutr;37:830-837.

      [68] Sanmugopriya,E and Venkataraman,S (2006): Studies on hepatoprotective and antioxidant actions of Strychnos potatorum Linn.seeds on CCl4 -induced acute hepatic injury in experimental rats. J. Ethnopharmacol. 105: 154-160.

      [69] Shaarawy SM, Tohamy AA Elgendy SM, Abd Elmageed ZY, Bahnasy A, Mohamed MS, Kandil E. and Matrougui K.(2009): Protective effects of garlic and silymarin on NDEA-induced rats hepatotoxicity. International Journal of Biological Sciences.5 (6):549-557.Ivyspring International Publisher.

      [70] Shahbazi F, Sadighi S, Dashti-Khavidaki S, Shahi F, Mirzania M, Abdollahi A. and Ghahremani MH.(2015): Effect of silymarin administration on cisplatin nephrotoxicity: report from a pilot, randomized, double-blinded, placebo-controlled clinical trial. Phytother Res. Jul; 29(7):1046-53.

      [71] Shahwan M, and Zain Al Abdin SM.(2018):Antioxidant, hepatoprotective and lipid lowering activity of Sarcopoterium Spinosum on carbon tetrachloride (CCl4)- induced hepatic damage in rats. J. Pharm. Sci. & Res. Vol. 10(11), 2800-2804.

      [72] Shen B, Chen H, Shen C, Xu P, Li J, Shen, G;Yuan, H and Han, J. (2015): Hepatoprotective effects of lignans extract from Herpetospermum caudigerum against CCl4-induced acute liver injury in mice. J Ethnopharmacol, 164: 46±52.

      [73] Shih CK, Chen SH, Hou WC and Cheng HH (2007): A high resistance starch rice diet reduces glycosylated hemoglobin level and improves the antioxidant status in diabetic rats. Food Research international volume 40, issue 7 pages 842-847.

      [74] Siti AZ and Sarbini SR. (2015): The potential of resistant starch as a prebiotic. Critical Reviews in Biotechnology 13(3):1-7.

      [75] Shukla A and Bhatia,S.J.(2010):Outcome of patients with primary hepatic venous obstruction treated with anticoagulants alone Indian J Gastroenterol 29(1):14–17.

      [76] Škottová N, Krečman V, Walterová D, Ulrichová J, Kosina P and Šimánek V (1998): Effect of silymarin on serum cholesterol levels in rats. Acta Univ. Palacki. Olomuc., Fac. Med. Volume 141, 1998

      [77] Skottová N, Vecera R, Urbánek K, Vána P, Walterová D, and Cvak L.(2003): Effects of polyphenolic fraction of silymarin on lipoprotein profile in rats fed cholesterol-rich diets. Pharmacol Res. J; 47(1):17-26.

      [78] Smazal AL, Borcherding NC Anderegg AS, Schalinske KL, Whitley EM and Rowling MJ (2013): Dietary resistant starch prevents urinary excretion of 25-Hydroxycholecalciferol and vitamin D-binding protein in type 1 diabetic rats. The journal of Nutrition, volume 143, issue 7. Pages 1123-1128.

      [79] Sobolova L, Skottova N, Vecera R and Urbanek K (2006): Effect of silymarin and its polyphenolic fraction on cholesterol absorption in rats. Pharmacol.Res., 53(2): 104-112.

      [80] Sonnenbichler J, Scalera F, Sonnenbichler I and Weyhenmeyer R. (1999): Stimulating effects of silibinin and silicristin from themilk thistle Silybum marianum on kidney cells. J Pharm Exp Ther, 290: 1375-1383,

      [81] SPSS Inc. Released (2009): PASW Statistics for Windows, Version 18.0. Chicago: SPSS Inc.SPSS Inc. Released (2009): PASW Statistics for Windows, Version 18.0. Chicago: SPSS Inc.

      [82] Storey D, Lee A, Bornet F and Brouns F. (2007): Gastrointestinal responses following acute and medium term intake of retrograded resistant maltodextrins,classifief as type 3 resistant starch. Eur.J.Clin. Nutr, 61:1262-1270.

      [83] Sueyoshi M, Fukunaga M, Mei M, Nakajima A, Tanaka G, Murase T,Narita Y, Hirata S and Kadowaki D.(2019): Effects of lactulose on renal function and gut microbiota in adenine-induced chronic kidney disease rats. Clin Exp Nephrol.; 23(7):908-919.

      [84] Taghiabadi E, Imenshahidi M, Abnous K, Mosafa F, Sankian M,Memar B and Karimi G. (2012): Protective effect of silymarin against acrolein-induced cardiotoxicity in mice.Evid Based Complement Alternat Med. 2012;2012:352091.

      [85] Takeiti CY, Kieckbusch TG and Collares-Queiroz FP. (2010): Morphological and physicochemical characterization of commercial maltodextrins with different degrees of dextrose-equivalent. Int. J.Food Propert.13:411-425.

      [86] Takahara E, Ohta S, Hirobe M. (1986) Stimulatory effects of silibinin on the DNA synthesis in partially hepatectomized rate livers. Biochemical Pharmacology 35, 538- 541.

      [87] Tayebi Khosroshahi H, Habibzadeh A, Khoshbaten M, Rahbari B, Chaichi P and Badiee AH.(2014): Lactulose for reduction of nitrogen products in patients with chronic kidney disease. Iran J Kidney Dis. Sep; 8(5):377-81.

      [88] Turgut F, Bayrak O, Catal F, Bayrak R, Atmaca AF, Koc A, Akbas A, Akcay A and Unal D.(2008): Antioxidant and protective effects of silymarin on ischemia and reperfusion injury in the kidney tissues of rats. Int Urol Nephrol.; 40(2):453-60.

      [89] Voroneanu L, Nistor I, Dumea R, Apetrii M and Covic A.(2016): Silymarin in Type 2 Diabetes Mellitus: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. J Diabetes Res.; 2:5147468.

      [90] Wu C T, Chiu CY, Huang CF, Peng FC and Liu SH.(2018): Genotoxicity and 28-day oral toxicity studies of a functional food mixture containing maltodextrin, white kidney bean extract, mulberry leaf extract, and niacin-bound chromium complex. Regul Toxicol Pharmacol. ;92:67-74


 

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Article ID: 30346
 
DOI: 10.14419/ijpt.v8i1.30346




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