Gastroprotective effect of ethylacetate fraction of the leaves of Hannoa klaineana on aspirin and histamine-induced gastric ulcer in rats

  • Authors

    • Ibrahim Abubakar Usmanu Danfodiyo University, Sokoto
    • Hassan Muhammad Yankuzo Usmanu Danfodiyo University Sokoto
    • Yusha'u Shuaibu Baraya Usmanu Danfodiyo University Sokoto
    • Mu'azu Abubakar Gusau Usmanu Danfodiyo University Sokoto
    2020-06-04
    https://doi.org/10.14419/ijpt.v8i1.30534
  • Aspirin, Gastric acid, Hannoa klaineana, Histamine, Prostaglandin.

  • Background: Peptic ulcer disease remains endemic in our society affecting about four million people every year worldwide. Hannoa klaineana is used traditionally in the treatment of various gastrointestinal diseases including ulcer.

    Aim: This study aims at evaluating the gastroprotective effect of ethylacetate fraction of the leaves of Hannoa klaineana (Simaroubaceae).

    Methods: The gastroprotective effect of ethylacetate fraction of the Hannoa klaineana (50, 100 and 200mg/kg b.wt) was evaluated using aspirin and histamine induced ulcer models.

    Results: In aspirin-induced ulcer model, the ethylacetate fraction of the Hannoa klaineana demonstrated significant (p<0.001) decreased in mean ulcer index with the maximum protective effect (99.84%) at 200 mg/kg against the gastric damages. While histamine-induced ulcer model, the solvent fraction significantly (p<0.001) decreased mean ulcer index with the protective effect up to 99.83% against the gastric lesions. In both models, a significant (p<0.001) increased in pH value coupled with significant (p<0.001) decreased in gastric volume, free and total acidity in rats pre-treated with varying doses of the ethylacetate fraction was found.

    Conclusion: The mechanism of gastroprotective effects of ethylacetate fraction of the Hannoa klaineana could be attributed to its ability to stimulate prostaglandins secretion or possess prostaglandins like-substances or suppression of histamine-induced vasospastic effect and gastric secretion.

     

     


     

  • References

    1. [1] Adinortey, M. B., Ansah, C., Galyuon, I. and Kwadwo, N. A. (2013). In vivo Models used for Evaluation of Potential Anti-gastroduodenal Ulcer Agents. Hindawi Publishing Corporation. p. 1-12.https://doi.org/10.1155/2013/796405.

      [2] Ahmad, A., Santosh, V. K. and Maurya, K. (2013). Natural Anti-ulcer Agents: A Pharmacological Review. Inter. J. Res. Pharmaceut. Biomed. Sci. 4(2):535-541.

      [3] Akah, P. A., Nnaeto, O., Nworu, C. S. and Ezike, A. C. (2007). Medicinal Plants Used in the Traditional Treatment of Peptic Ulcer Diseases: A Case Study of Napoleona vogelii Hook and Planch (Lecythidaceae). Res. J. Pharmacol. 1(3): 67-74.

      [4] Almeida, L. L. A., Rolim, L. A., Santos, V. L. and Wanderley, A. G. (2017). Spondias purpurea L. (Anacardiaceae): Antioxidant and Antiulcer Activities of the Leaf Hexane Extract. Oxid. Med. Cell. Longev., p 1–14.https://doi.org/10.1155/2017/6593073.

      [5] Amagase, K. and Okabe, S. (2003). On the Mechanisms Underlying Histamine Induction of Gastric Mucosal Lesions in Rat with Partial Gastric Vascular Occlusion. J. Pharmacol. Sci. 92: 124-136.https://doi.org/10.1254/jphs.92.124.

      [6] Andrade-Neto, V. F., Pohlit, A. M., Pinto, A. C., Silva, E. C., Nogueira, K. L., Melo, M. R., Henrique, M. C., Amorim, R. C., Silva, L. F., Costa, M. R., Nunomura, R. C., Nunomura, S. M., Alecrim, W. D., Alecrim, M. D., Chaves, F. C. and Vieira, P. F. (2007). In vitro Inhibition of Plasmodium Falciparum by Substances Isolatade from Amazonian Antimalarial Plants. Mem. I. Oswaldo Cruz 102: 359-365.https://doi.org/10.1590/S0074-02762007000300016.

      [7] Ang, H. H., Chan, K. L. and Mak, J. W. (1995). In vitro Antimalarial Activity of Quassinoids from Eurycomalongifolia against Malaysian Chloroquine-Resistant Plasmodium Falciparum Isolates. PO1 Planta Med. 61: 177-178.https://doi.org/10.1055/s-2006-958042.

      [8] Basilevskaia, V. (1969). Pluntes Mbdicinales de Guine’e. Conakry, Republique de Guinee, p. 126.

      [9] Bhala, N., Emberson, J. and Merhi, A. (2013). Vascular and Upper Gastrointestinal Effects of Non-Steroidal Anti-Inflammatory Drugs: Meta-Analyses of Individual Participant Data from Randomised Trials. Lancet. 382 (98):769–779. https://doi.org/10.1016/S0140-6736(13)60900-9.

      [10] Bharti, S., Wahane, V. D. and Kumar, V. L. (2010). Protective Effect of Calotropisprocera latex Extracts on Experimentally Induced Gastric Ulcers in Rat. J. Ethnopharmacol. 127: 440-444.https://doi.org/10.1016/j.jep.2009.10.016.

      [11] British National Formulary(BNF) (2018). 76 (76 ed.). Pharmaceutical Press. p. 73.

      [12] Das, D., Bandyopadhyay, D., Bhattacharjee, M. and Banerjee, R. K. (1997). Hydroxyl Radical is the Major Causative Factor in Stress-Induced Gastric Ulceration. Free Radical Biology and Medicine. 23(1): 8-18.https://doi.org/10.1016/S0891-5849(96)00547-3.

      [13] Datta, G. K., Sairam, K., Priyambada, S., Debnath, P. K. and Goel, R. K. (2002). Antiulcerogenic Activity of Satavarimandw: An Ayurvedicherbo Medical Preparation. Indian J. Exp. Biol. 40: 1173-1177.

      [14] Del, B. T., Borgoni, R., Del, B. P., Edaro, P., Vianello, F. and Danieli, G. A. (2003). Peptic Ulcer Inheritance in Patients with Elevated Serum Pepsinogen Group Levels and without Infection of Helicobacter pylori. Dig. Liver Dis. 32: 12.https://doi.org/10.1016/S1590-8658(00)80038-9.

      [15] Devi, K. N., Raja, N. R. L., Palanivelu, M., Srinivasan, B., Rajarathinam, D., Kiran, C. K. and Niranjan, P. (2011). Anti-ulcer activity of the Leaves of Punica granatum Linn. Asian J. Biochem. Pharmaceut. Res. 4(1):116-122.

      [16] Esplugues, J., Lloris, J. M., Marti-Bonmati, E. and Morcillo, E. J. (1982). Effects of Adrenoceptor Drug Stimulation on Various Models of Gastric Ulcer in Rats. Br. J. Pharmacol. 76: 587–94.https://doi.org/10.1111/j.1476-5381.1982.tb09258.x.

      [17] Feldman, M., Friedman, L. S. and Brandt, L. J. (2016). Sleisenger and Fordtran’s Gastrointestinal and Liver Disease: Pathophysiology/Diagnosis/Management. 10th ed Philadelphia, PA: Saunders/Elsevier. 2: 2369-2389.

      [18] Fiocca, F., Basso, N. and Passaro, E. P. (1974). Effect of Histamine on Isolated Human Gastric Mucosa. Surg. Forum 25:321-323.

      [19] Gaskill, H. U., Sirinek, K. R. and Levine, B. A. (1982). Effect of Prostacyclin on Mucosal Blood Flow. Surgery 92: 220-225.

      [20] Glarborg J. T., Weis-Fogh, U. S. and Neilsen, H. H. (1976). Salicylate- and Aspirin-Induced Uncoupling of Oxidative Phosphorylation in Mitochondria isolated from the Mucosal Membrane of the Stomach. Scand J. Lab. Invest. 36: 649–653.https://doi.org/10.3109/00365517609054490.

      [21] Goroll, A. H. and Mulley, A. G. (2009). Primary Care Medicine. Philadelphia, 6: 537– 548.

      [22] Hawkey, C. J. and Ramton, D. S. (1985). Prostaglandins and the Gastrointestinal Mucosa. Are They Important in Its Function, Disease or Treatment? Gastroenterology. 89: 1162-1168.https://doi.org/10.1016/0016-5085(85)90225-2.

      [23] Hay, L. J., Varco, C. F., Code, O. F. and Wangensteen, R. L. (1942). “Experimental Production of Gastric and Duodenal Ulcers in Laboratory Animals by Intramuscular Injection of Histamine in Bees wax,†The Journal of Surgery, Gynecology and Obstetrics. 74: 70–182.

      [24] Hogan, D. L., Ainsworth, M. A. and Isenberg, J. I. (1994). Review Article: Gastroduodenal Bicarbonate Secretion. Aliment Pharmacol. Ther. 8(5):475-488. https://doi.org/10.1111/j.1365-2036.1994.tb00319.x.

      [25] Jhasnsi, R. M., Mohana, I. S. and Saravana, K. A. (2010). Review on Herbal Drug For Anti-Ulcer Propetrty. International Journal of Biological and Pharmaceutical Research ICID. 20-26.

      [26] Jyoti, G., Dinesh, K. and Ankit, G. (2012). Evaluation of Gastric Anti-Ulcer Activity of Methanolic Extract of Cayratiatrifoliain Experimental Animals. Asian Pacific J. Trop. Dis, 1: 99-102.https://doi.org/10.1016/S2222-1808(12)60024-3.

      [27] Klein-Junior, L. C., Gandolfi, R. B., Santin, J. R., Lemos, M., Cechinel, F. V. and Andrade, S. F. (2010). Antiulcerogenic Activity of Extract, Fractions, and some Compounds obtained from Polygola cyparissias St Hillaire & Moquin (Poligalaceae). Naunyn Schmiedebergs Arch. Pharmacol. 381:121-126. https://doi.org/10.1007/s00210-009-0485-x.

      [28] Klein-Júnior, L. C., Santin, J. R., Niero, R., deAndrade, S. F. and Cechinel-Filho, V. (2012). The Therapeutic Lead Potential of Metabolites obtained from Natural Sources for the Treatment of Peptic Ulcer. Phytochem. Rev. 11(4): 567–616.https://doi.org/10.1007/s11101-012-9262-4.

      [29] Kulkarni, S. K. (2002). Hand Book of Experimental Pharmacology, Vallabh Prakashan, New Delhi, India, 3rd ed. Remedies. Phytother. Res. 14:581-591.

      [30] Kumar, A., Dewan, B. and Rama, T. (2011). Evaluation of Anti-ulcerogenic Properties from the root of Flemingia strobilifera. J. Basic Clin. Pharm. 2(1): 33-39.

      [31] Lüllmann, H., Mohr, A., Ziegler, D. and Bieger, K. (2000). Color Atlas of Pharmacology, 2nd ed., Thieme Stuttgart, New York, pp. 166.

      [32] Maity, S., Vedasiromoni, J. R. and Ganguly, D. K. (1995). Anti-ulcer Effect of the Hot Water Extract of Black Tea (Camellia sinensis). Journal of Ethnopharmacology. 46: 167 – 174.https://doi.org/10.1016/0378-8741(95)01245-9.

      [33] Malairajan, P., Gopalkrishnan, G., Nrasimhan, S., Jessi, K., Veni, K. and Kavimani, S. (2007). Anti-ulcer Activity of Crude Alcoholic Extract of Toonaciliata roemer (heart wood). J. Ethnopharmacol. 110: 348–57.https://doi.org/10.1016/j.jep.2006.10.018.

      [34] Ministry of Food Drug Safety (MFDS). Food Code. Korean Foods Industry Association, Seoul, Korea. 2014.

      [35] Monjour, L., Rouquier, F., Alfred, C. and Polonsky, J. (1987). Essais De traitement Du paludismemurin Experimental Par un Quassino’ide, Laglaucarubinone. CRAcad Sci. Paris, III. Sciences dela Vie 304: 129-132.

      [36] Moody, F. G. and Davis, W. L. (1970). Hydrogen and Sodium Permeation of Canine Gastric Mucosa during Histamine and Sodium Thiocyanate Administration. Gastroenterology 59:350-357.https://doi.org/10.1016/S0016-5085(19)33728-X.

      [37] Muralidharan, P. and Srikanth, J. (2009). Antiulcer Activity of Morinda citrifolia Linn fruit Extracts. J. Sci. Res., 1(2): 345–352.https://doi.org/10.3329/jsr.v1i2.1625.

      [38] Nwafor, S. V. and Okoye, C. F. (2005). Antiulcer Properties of Ethanol Root Extract of Cissampelos Mucronata. Pharm. Biol. 43 (5):396-403.https://doi.org/10.1080/13880200590963222.

      [39] Nwinyl, F. C. and Kwanashie, S. (2013). Comparative Effects of Sorghum bicolor Leaf Base Extract on Tissues Isolated from Some Body System of Experimental Animals. J. Med Plants Res, 7 (41): 3041-305.

      [40] O'Brien, P. and Silen, W. (1973). Effect of Bile Salts and Aspirin on the Gastric Mucosal Blood Flow. Gastroenterology 64:246-253.https://doi.org/10.1016/S0016-5085(73)80036-8.

      [41] Odeghe, O. B, Monanu, M. O. and Anacletus F. C. (2016). In vitro Antioxidant Abilities and Inhibition of Rabbit Muscle Lactate Dehydrogenase by Aqueous Extract of Hannoa klaineana stem bark. International Journal of Advanced Research 4 (2): 509-515.

      [42] Proctor, M. J. and Deans, C. (2014). Complications of Peptic Ulcers. Surgery (Oxford). 32: 599-607.https://doi.org/10.1016/j.mpsur.2014.09.005.

      [43] Rajkapoor, B., Anandan, R. and Jayakar, B. (2002). Anti-ulcer Effect of Nigella saliva Linn Against Gastric Ulcer in Rats. Current Science. 82: 177 – 179.

      [44] Robert, A., Nezamis, J. E., Lancaster, C. and Hanchar, A. J. (1979). Cytoprotection by Prostaglandins in Hypertonic NaCI and Thermal Injury. Gastroenterology. 77:433-443.https://doi.org/10.1016/0016-5085(79)90002-7.

      [45] Sánchez-Mendoza, M. E., Reyes-Trejo, B., Sánchez-Gómez, P., Rodríguez-Silverio, J., Castillo-Henkel, C., Cervantes-Cuevas, H.and Arrieta, J. (2010). Bioassay-Guided Isolation of an Anti-Ulcer Chromene from Eupatorium Aschenbornianum: Role of Nitric Oxide, Prostaglandins and Sulfydryls. Fitoterapia. 81: 66–71.https://doi.org/10.1016/j.fitote.2009.07.009.

      [46] Sander, L. E., Lorentz, A., Sellge, G., Coeffer, M., Neipp, M. and Veres, T. (2006). Selective Expression of Histamine Receptors H1R, H2R and H4R, but not H3R, in the Human Intestinal Tract. J. Gut. 55: 498-504. https://doi.org/10.1136/gut.2004.061762.

      [47] Shan, L., Liu, R. H., Shen, Y. H., Zhang, W. D., Wu, C. Z. and Min, L. (2006). Gastroprotective Effect of a Traditional Chinese Herbal Drug “Baishouwu†on Experimental Gastric Lesions in Rats. J. Ethnopharmacol. 107: 389–94.https://doi.org/10.1016/j.jep.2006.03.022.

      [48] Shay, H., Sun, D. C. H. and Gruenstein, M. (1954). A Quantitative Method for Measuring Spontaneous Gastric Secretion in the Rat. Gastroenterol, 26: 906-913.https://doi.org/10.1016/S0016-5085(54)80008-4.

      [49] Spenny, J. G. and Bhown, M. (1977). Effect of Prostaglandin Acid on Gastric Mucosa II. Mucosal ATP and Phosphocreatinine Content and Salicylic Effects on Mitochondrial Meta- bolism. Gastroenterology. 73:995–999. https://doi.org/10.1016/S0016-5085(19)31912-2.

      [50] Szabo, S., Trier, J. S. and Frankel, P. W. (1981). Sulfhydryl Compounds May Mediate Gastric Cytoprotection. Science 214:200-202.https://doi.org/10.1126/science.7280691.

      [51] Takeuchi, K. and Nobuhara, Y. (1981). Inhibition of Gastric Motor Activity of 16,16-dimethyl prostaglandin E: A Possible Explanation of Cytoproteciion. Dig. Dis. Sci. 30:1181-1188. https://doi.org/10.1007/BF01314054.

      [52] Toruner, M. (2007). Aspirin and Gastrointestinal Toxicity. Journal of Anatomical Cardiology. 24 (l): 27 - 30.

      [53] Wallace, J. L. (2008). Prostaglandins, NSAIDs, and Gastric Mucosal Protection: Why Doesn’t The Stomach Digest Itself? Physiol. Rev. 88:1547–1565.https://doi.org/10.1152/physrev.00004.2008.

      [54] Whittle, B. J. (2003). Gastrointestinal Effects of Non-steroidal Anti-inflammatory Drugs. Fundam Clin. Pharmacol. 7:301–13.https://doi.org/10.1046/j.1472-8206.2003.00135.x.

      [55] Whittle, B. J. R. (1977). Mechanism Underlying Gastric Mucosal Damage Induced by Indomethacin and Bile Salt, and the Actions of Prostaglandins. Br. J. Pharmacol. 60: 455–460.https://doi.org/10.1111/j.1476-5381.1977.tb07522.x.

      [56] Whittle, B. J. R., Vane, J. R., Botting, R. M. (1992). Unwanted Effects of Aspirin and Related Agents on the Gastrointestinal Tract. Aspirin and other Salicylates. London: Chapman and Hall Medical. 465–509.

      [57] Zelickson, M. S., Bronder, C. M., Johnson, B. L., Camunas, J. A., Smith, D. E., Eawlinson, D., Von, S., Stone, H. H. and Taylor, S. M. (2011). Helicobacter pylori is not the Predominant Etiology for Peptic Ulcers Requiring Operation. Am. Surgeon J. 77:1054-1060.

      [58] Zhang, L., Ren, J. W., Wong, C. C., Wu, W. K., Ren, S. X., Shen, J., Chan, R. L. and Cho, C. H. (2014). Effects of Cigarette Smoke and Its Active Components on Ulcer Formation and Healing in the Gastrointestinal Mucosa. Curr. Med. Chem. 19: 63-69.https://doi.org/10.2174/092986712803413926.

  • Downloads

    Additional Files

  • How to Cite

    Abubakar, I., Muhammad Yankuzo, H., Shuaibu Baraya, Y., & Abubakar Gusau, M. (2020). Gastroprotective effect of ethylacetate fraction of the leaves of Hannoa klaineana on aspirin and histamine-induced gastric ulcer in rats. International Journal of Pharmacology and Toxicology, 8(1), 70-77. https://doi.org/10.14419/ijpt.v8i1.30534