Assessment of the total aqueous stem bark extract of Sacoglottis gabonensis (Baille) Urban on the anatomo-histology of Wistar rat liver, kidneys and heart

 
 
 
  • Abstract
  • Keywords
  • References
  • PDF
  • Abstract


    Sacoglottis gabonensis is a medicinal plant used traditionally for the treatment of Buruli ulcer in Côte d’Ivoire. To ensure its effect over a long period of use, assessment of the total aqueous stem bark extract of S. gabonensis (ETASg) on anatomo-histology of Wistar rat liver, kidneys and heart was evaluated. ETASg was administered to Wistar rats at doses of 3.5; 35 and 350 mg/kg/day for 90 days. The liver of rats treated with ETASg at 35 and 350 mg/kg showed color and appearance changes with whitish nodules. Furthermore, histological architecture revealed micro-vesicular steatosis. In contrast, gross examination did not showed any change color and appearance of the kidneys and heart of the rats tested at all doses of ETASg compared to those of control rats. Histological sections of the kidneys and heart of rats tested at all doses of ETASg showed normal kidney and heart cells. After stopping the administration, the liver abnormalities disappeared at the end of the 120 days. This study revealed that the integrity of the liver tissue is preserved only with ETASg of 3.5 mg/kg. On the other hand, it is guaranteed at all the doses studied for the renal and cardiac tissues.

     



  • Keywords


    Anatomo-Histological; Heart. and Kidneys; Liver; Rats; Sacoglottis gabonensis.

  • References


      [1] Aubréville A (1959). The forest flora of Côte d'Ivoire. 2nd revised edition, Volume 3, Publication No 15 of the Tropical Forestry Center, France, pp 66-70.

      [2] Chandrasoma P, Taylor CR (1997). Concise pathology. 3rd Edition, McGraw-Hill Singapore, 317p.

      [3] Debelo N, Afework M, Debella A, Makonnen E, Ergete W, Geleta B (2016). Assessment of hematological, biochemical and histopathological effects of acute and sub-chronic administration of the aqueous leaves extract of Thymus schimperi in rats. Journal of Clinical Toxicology 6(2) : 1-9 https://doi.org/10.4172/2161-0495.1000286.

      [4] Fennell CW, Lindsey KL, McGaw LJ, Sprag SG, Stafford GI, Elgoraschi EE, Grace OM, Van Staden J (2004). Assessing African medicinal plants for efficacy and safety: pharmacological screening and toxicology. Journal of Ethnopharmacology 94 : 205-217 https://doi.org/10.1016/j.jep.2004.05.012.

      [5] Fromenty B, Pessayre D (1995). Inhibition of mitochondrial bêta-oxidation as a mechanism of hepatotoxicity. Pharmacology Thermal (67) : 101-154 https://doi.org/10.1016/0163-7258(95)00012-6.

      [6] Hould R (1984). Histopathology and cytopathology technique, Edition Maloine, Paris-France, 399 p.

      [7] Kognou MLA, Tchamgoue DA, Tchokouaha YRL., Nthenge-Ngumbau ND, Fokou TVP, Tchinda TA, Agbor AG, Etame EMR, Mouokeu SR, Gueiffier EC, Pawar SR, Mouelle SA, Ngane NAR (2018). Acute and sub-chronic toxicity studies of Dichaetanthera africana (Hook. F.) Jacq. Fel. (Melastomataceae) stem bark ethanol extract. Journal of Applied Pharmaceutical Science 8(6): 147-155 https://doi.org/10.7324/JAPS.2018.8619.

      [8] Koné M, Bléyéré NM, Yapo AP, Vangah MO, Ehile EE (2009). Evaluation of the toxicity of an aqueous extract of Sacoglottis gabonensis (Baille) Urban (Humiriaceae) in rodents, a plant used in the treatment of Buruli ulcer in Côte d’Ivoire. International Journal of Biology Chemical Science 3(6) : 1286-1296 https://doi.org/10.4314/ijbcs.v3i6.53147.

      [9] Koné M, Vangah-Manda OM, Kouakou H, Yapo AP, Bléyéré NM, Datté YJ (2007). Influence of Sacoglottis gabonensis (Baille) Urban and Okoubaka aubrevillei Normand and Pellegrin on the in vitro growth of Mycobacterium ulcerans. Medicine Black Africa 54(11) : 549-554.

      [10] Kouassi KB, Adjambri AE, Koné M, Sawadogo D, Yapo AP (2018). Influence of an aqueous extract of Sacoglottis gabonensis (Baille) Urban (Humiriaceae) stem bark, a plant used in the traditionnal treatment of Buruli ulcer, on anthropometric and hematological parameters in wistar rat. The Pharma Innovation Journal 7(3) : 104-110.

      [11] Legbosi LN, Ellis RT (2018). Sub-chronic toxicity of hydromethanolic stem bark extract of Musanga cecropioides (Urticaceae) in rat. EC Pharmacology and Toxicology 6(3) : 76-95.

      [12] Martin E, Feldmann G (1983). Histopathology of the liver and bile ducts in adults and children, Edition Masson, France, 357 p.

      [13] Martoja R, Martoja-Pierson M (1967). Introduction to the techniques of histology. Edition Masson et Cie, Paris, France, 345p.

      [14] Nassir F, Rector SR, Hammoud GM, Jbdah JA (2015). Pathogenesis and Prevention of Hepatic Steatosis. Gastroenterrology and Hepatology 11(3): 167-175. PMID: 27099587; PMCID: PMC4836586.

      [15] Nene-Bi SA, Ramachandran V, Rao PV, Krishnan RRG, Dhanabal SP, Traore F (2016). Subchronic toxicity studies of the aqueous stem bark extract of Bridelia ferruginea in Wistar rats. Bulletin of Environment. Pharmacology and Life Sciences 5(10): 14-21.

      [16] Nortier J, Depierreux M, Vanherweghem JL (1999). Phytotherapy and nephrotoxicity. Journal of Medicine Brussels 1 : 9-14.

      [17] OECD (1998). OECD Guideline for the Testing of Chemicals: Repeated-dose oral toxicity to rodents, 90 days. OECD 408, 16 p.

      [18] Otis TBI, Kouassi KB, Gnangoran BN, Kone M, Yapo AP (2018). Subchronic Toxicity Study of the Extract of Sacoglottis gabonensis (Baille) Urban (Humiriaceae) in Wistar Rats. International Journal of Biochemistry Research & Review 24(1) : 1-10 https://doi.org/10.9734/IJBCRR/2018/44353.

      [19] Peyrin-Biroulet L, Barraud H, Petit-Laurent F, Ancel D, Watelet J, Chone L, Hudziak H, Bigard MA, Brenowicki JP (2004). Hepatotoxicity of herbal medicine: clinical, biological data, histological and mechanisms involved for some typical examples. Clinical and Biological Gastroenterology 28(7): 540-550 https://doi.org/10.1016/S0399-8320(04)95009-9.

      [20] Rocquelin G (1979). Pathology research in rats ingesting different doses of peanut oil or rapeseed oil with low erucic acid content (Huile de colza Primor): Animal growth and organ weights. Annals of animal biology, biochemistry, biophysics, pp 483-485. https://doi.org/10.1051/rnd:19790403.

      [21] Scoazec JY (1999). Hepatic endothelial cells: an unexpected diversity and its physiological and pathophysiological consequences. Hepato-gastrology and digestive oncology 6(3): 209-220.

      [22] Udeh EN, Anaga A, Asuzu UI (2018). Acute and sub-chronic oral toxicity studies on methanol leaf extract of Gnetum africanum Welw in Wistar rats. American Journal of Research in Medical Sciences 3(1): 7-14. https://doi.org/10.5455/ajrms.20180109094114.

      [23] Vangah OM, Kroa E, Zai LP (2000). Census of traditional health practitioners, practices, pathologies and medicinal plants of Côte d'Ivoire. TV: Lakes Region: Toumodi Department, Yamoussoukro, Tiébissou and Didiévi Sub-Prefecture. WHO-CI Technical Report, 40 p.

      [24] World Health Organization (WHO) (2008). Traditional medicine. Fact sheet N°134. WHO/EDM/TRM, Genève-Suisse, 79 p.


 

View

Download

Article ID: 31278
 
DOI: 10.14419/ijpt.v9i1.31278




Copyright © 2012-2015 Science Publishing Corporation Inc. All rights reserved.