Hepatorenal protective effect of ocimum sanctum in chickens toxicated by gentamicin

  • Authors

    • Hadeer A. M. Abdul Ghaffar Benha university
    • Ashraf A. A.Elkomy
    • Enas A.H. Farag
    2021-03-19
    https://doi.org/10.14419/ijpt.v9i1.31421
  • Hepatotoxicity, Nephrotoxicity, Ocimum sanctum, Chickens, Gentamicin.

  • Background: ocimum sanctum (Tulsi) “Queen of herbs†is considered as sacred and medicinal plant in a lot of countries around the world especially India. Therefore this study was carried out to investigate the heptorenal protective effect of ocimum sanctum aqueous extract against gentamicin induced hepatorenal toxicity in chickens.

    Materials and Methods: A total 100, one-day old unsexed broiler chicks of a commercial breed (Cobb breed) were used in this study, the chickens were subjected to different treatments blood and tissue samples were collected at 5th week of age post administration, biochemical and histopathological examinations were utilized to investigate protective effect of ocimum sanctum aqueous extract on liver and kidney.

    Results: gentamicin recorded significant (P<0.05)increase in serum level of aspartate aminotransferase (AST), alanine aminotransferase (ALT), uric acid and creatinine levels when compared with control group, the administration of ocimum sanctum aqueous extract with gentamicin ameliorate their toxic effect, the group treated with ocimum sanctum aqueous extract only similar to control, while gentamicin induce significant (P<0.05)decrease in serum albumin, total protein and globulin levels when compared with control group, ocimum sanctum aqueous extract with gentamicin restore their toxic effect. The group treated with ocimum sanctum aqueous extract only as control. Gentamicin induce histopathological alterations in liver and kidney tissues by comparison with control group and ocimum sanctum aqueous extract treated group as control, while the administration of ocimum sanctum aqueous extract with gentamicin showing improvement in histopathological lesion compared with toxic effect induced by gentamicin.

    Conclusions: The hepatotoxicity and nephrotoxicity induced by gentamicin were ameliorated by aqueous extract of ocimum sanctum especially at dose of 2ml/liter in drinking water for 12 days.

     

     

     

  • References

    1. [1] Abou Elazab MF and El-Habashi N (2015), Gentamicin-induced nephrotoxicity in chickens: Modulatory Role Of Moringa Oleifera. Vol. 61, No. 144.Fauci AS, Braunwald E, Kasper DL & Hauser SL (2008), Principles of Harrison’s Internal Medicine, Vol. 9, 17thedn. McGraw-Hill, New York, NY, pp.2275–2304.

      [2] Al-Naqeeb MA, Thomson M, Al-Qattan K, Kamel F, Mustafa T and Ali M (2003), Biochemical and histopathological toxicity of an aqueous extract of ginger in female rats. Kuwait journal of science and engineering 30, 35-48.Lee JR, Kim SA, Yoo JW & Kang YK (2007), The present status of diabetes education and the role recognition as a diabetes educator of nurses in korea. Diabetes Research and Clinical Practice 77, 199–204.

      [3] Alom F, Mostofa M, Alam M, Sorwar M, Uddin J and Rahman M (2015), Effects of indigenous medicinal plant Tulsi (Ocimum sanctum) leaves extract as a growth promoter in broiler. Research in Agriculture Livestock and Fisheries, vol. 2, issu(1),pp. 97-102.

      [4] Anonymous (1991), Wealth of India. Publication and Information Directorate, CSIR, New Delhi. Vol. (7); p. 79–89.

      [5] Arafat N, EL-Shafei R, Awadin W, Farag V and Saleh R (2017), Consequencs of hepatorenal toxicity induced by gentamicin in chickens. Vol. 63 No. 154, pp 1-12.

      [6] Arafat N, AwadinWF, El-Shafei RA, Farag VME and Saleh RM (2018), Protective Role of Moringa oleifera Leaves Extract Against Gentamicin-induced Nephro- and Hepato- Toxicity in Chickens. Vol. 58 (1): 173-185.

      [7] Arivuchelvan A, Murugesan S, Mekala P and Yogeswari R (2012), Immunomodulatory Effect of Ocimum sanctum in Broilers Treated with High Doses of Gentamicin.

      [8] Aronson JK (2016), Meyler's Side Effects of Drugs (Sixteenth Edition) The International Encyclopedia of Adverse Drug Reactions and Interaction, Pages 216-236.

      [9] Bharavi K, Gopala Reddy A, Rao GS, Ravi Kumar P, D Srinivas Kumar, P Prabhu Prasadini (2011), Prevention of cadmium bioaccumulation by herbal adaptogens. Indian J Pharmacol. Vol.43(1): pp. 45-49.

      [10] Center SA (2007), Interpretation of liver enzymes. Vet. Clin. North Am. Small Anim. Pract. 37(2), 297-333.

      [11] Doumas BT, Watson WA and Biggs HG (1971), Albumin standards and the measurement of serum albumin with bromcresol green. Clin Chim Acta. Vol. 31: pp. 87-96.

      [12] EL-Zoghby R, Abo-Kora S and Amin A (2013), Pathological And Clinicopathological Studies on The Effect of Gentamicin And I or Cefotaxime on Chicken. Vol. 26, No.2, pp. 89- 105.

      [13] Gupta G and Charan S (2007), Exploring the potentials of Ocimum sanctum ( Shyama tulsi) as a feed supplement for its growth promoter activity in broiler chickens. Vol. (42), issue (2).pp. 140-143.

      [14] Gupta SK, Prakash J and Srivastava S (2002), Validation of traditional claim of Tulsi, Ocimum sanctum Linn. as a medicinal plant. Indian J Exp Biol. Vol. 40 (7): pp. 765–773.

      [15] Haritova AM, Djeneva HA, Lashev LD, Sotirova PG, Gurov BI and Dyankov VN (2004), Pharmacokinetics of gentamicin and apramycin in tukeys roosters and hens in the context of pharmacokinetic–pharmacodynamic relationships. J. Vet. Pharm. Ther. 27(5), 381-384.

      [16] Islam NU, Khan MZ, Saleemi MK, Khan A, Bhatti SA, Yousaf M and Hassan ZU (2011), Clinicopathological studies on gentamicin toxicity in White Leghorn commercial layers. Pak. Vet. J. 31(4), 305-308.

      [17] Javed U, Khan MZ, Saleemi MK, Ahrar Khan, Javed I and Rafique S (2013), Toxico-pathological Effects of Parenteral Administration of Gentamicin in Growing Broilers. Vol 15: issu (3), pp 529‒534.

      [18] Kang C, Lee H, Hah DY, Heo JH, Kim CH, Kim E and Kim JS (2013), Protective effects of Houttuynia cordata Thunb. on gentamicin- induced oxidative stress and nephrotoxicity in rats. Toxicol. Res. 29(1), 61-67.

      [19] Khan I, Khan M, Kashif M and Javed I (2008), Pathological and Biochemical Effects of Intramuscular Gentamicin Administration in Chickens. Vol. 32(5): pp.345-351.

      [20] Koller A (1984), Total serum protein. Clin. Chem. The C.V. Mosby Co. St Louis. Toronto. Princeton. Pp. 1316-1324.

      [21] Kumari M (2012), Clinico-pathological and Immunological studies in Escherichia coli infected broiler chicks fed on Ocimum sanctum leaf supplemented feed.

      [22] Laureat G, Maldague P, Carlier MB and Tulkens PM (1983), Increased renal DNA synthesis in vivo after administration of low doses gentamicin to rats. Antimicrob. Agent Chemother. 24(4), pp. 586-593.

      [23] Lobo V, Patil A, Phatak A and handra N (2010), Free radicals, antioxidants and functional foods: impact on human health. Pharmacog. Rev. 4(1), 18-26.

      [24] Masood A, Nadeem A, Mustafa SJ and O’Donnell JM (2008), Reversal of oxidative stress-induced anxiety by inhibition of phosphodiesterase-2 in mice. J. Pharmacol. Exp. Ther. 326(2), 369-379.

      [25] Murray R and Kaplan A (1984), Aspartate aminotransferase. Clin. Chem. The C.V. Mosby Co. St Louis. Toronto. Princeton. Pp. 1112-1116.

      [26] Murray R.L. (1984), Creatinine. Clin. Chem. The C.V. Mosby Co. St Louis. Toronto. Princeton. Pp.1261-1266 and 418.

      [27] Prakash P and Gupta N (2005), Therapeutic uses of Ocimum sanctum Linn (Tulsi) with a note on eugenol and its pharmacological actions: a short review. Indian J Physiol Pharmacol. Vol. (49): pp.125-131.

      [28] Saleemi MK, Khan MZ, Javed I and Khan A (2009), Pathological effects of gentamicin administered intramuscularly to day-old broiler chicks. Volume 61, Issue 5, Pages 425-432.

      [29] Schmidt RE, Reavill DR and Phalen DN (2003), Urinary system. In: Pathology of pet and aviary birds. Ames (IA): Iowa State Press, 95-107.

      [30] Shin HS, Yu M, Kim M, Choi HS and Kang DH (2014), Renoprotective effect of red ginseng in gentamicininduced acute kidney injury. Lab. Invest. 94(10), 1147- 1160.

      [31] SPSS Inc. Released (2009), PASW Statistics for Windows, Version 18.0. Chicago: SPSS Inc.

      [32] Strader DB, Bacon BR, Lindsay KL, La Brecque DR, Morgan T, Wright EC, Allen J, Khokar MF, Hoofnagle JH and Seeff LB (2002), Use of complementary and alternative medicine in patients with liver disease. Am. J. Gastroenterol. 97, 2391-2397

      [33] Tennant BC and Center SA (2008), Hepatic function. In Kaneko J.J., Harvey J.W., BrussM.L. (Eds.), Clinical Biochemistry of Domestic Animals. Sixth Edition. Academic Press; New York; p.379-412.

      [34] Verma S (2016), Chemical constituents and pharmacological action of Ocimum sanctum (Indian holy basil-Tulsi). 5(5): 205-207.

      [35] White BA, Erickson MM and Stevens SG (1970), Colorimetric determination of uric acid. Chemistry for medical technologists 3rd ed C. V. Mosby Company, saint Louis,U .S.A.P(662)

      [36] Yildirim BA, Tunc MA, Yildirim S, Yildirim F, Kordali S, Ertekin A and Aktas Senocak E (2017), The effect of Vitis vinifera L. cv. Merlot seed extract on gentamicin-induced nephrotoxicity in broilers. Vol. 81, pp. 1-12

  • Downloads

  • How to Cite

    A. M. Abdul Ghaffar, H., A. A.Elkomy, A., & A.H. Farag, E. (2021). Hepatorenal protective effect of ocimum sanctum in chickens toxicated by gentamicin. International Journal of Pharmacology and Toxicology, 9(1), 50-55. https://doi.org/10.14419/ijpt.v9i1.31421