Ameilorative effect of lycopine against cisplatin toxicity in rats
Keywords:Cisplatin, Lycopine, Liver, Brain.
The following study aimed to investigate the hepato and neuro protective efficacy of Lycopine against Cisplatin which induced hepatotoxicity and neurotoxicity. Twenty Five male Wister rats were used for this experiment they were equally divided into 5 groups (5 rats per group): group (1) served as control group they were injected 1ml saline orally once daily for 20 day, group (2) served as Corn Oil group and they were administrated 1 mL Corn Oil orally once daily for 20 days, group (3) served as Lycopine group and they were administrated (10 mg/kg b.wt) Lycopine orally once daily for 20 days. , group (4) served as Cisplatin treated group and they were injected (6 mg/kg b.wt.) intrapertonialy once at day 10 of experiment and group (5) Lycopine+Cisplatin group and were administrated 10 mg/kg b.wt Lycopine orally once daily for 20 days and injected 6 mg/kg b.wt.) intraperitonialy once at day 10 . Result revealed that Cisplatin induced liver damage indicated by significant increase in liver biomarkers ALP, AlT, AST along with significant decrease in albumin, Moreover marked increase increase in tissue concentrations of malondialdehyde(MDA) and Total antioxidant(TAC) and reduce tissue Glutathione reductase(GSH),that indicated oxidative stress Also results revealed up regulation IL-6 and down regulation IL-10 in liver and brain tissue in compared to control group . However, interestingly concurrent adminsteration of the Lycopine orally at dose level of 10mg/kg b.wt for 20 days with Cisplatin can mitigate these toxic effects caused by Cisplatin.So it is concluded that the antioxidant and the anti-inflammatory effects of Lycopine moderate the Cisplatin-induced hepato and neurotoxicity.
 AL-Salmi F.A.(2019): Ameliorative and Synergistic Effect of Red Raspberry and Lycopine against Hepatotoxicity Induced by Acrylamide in Male Mice. International Journal of Pharmacology, 15: 166-76, 2019. https://doi.org/10.3923/ijp.2019.166.176.
 Ammar A, Hadi, Sabah N, and Al T (2013): Evaluation of the Protective Properties of Amlodipine, on Cisplatin Induced Cardiotoxicity in Male Rats Evaluation of the Protective Properties of Amlodipine, on Cisplatinn Induced Cardiotoxicity in Male Rats Evaluation of the Protective Properties of Amlodipine, on Cisplatin Induced Cardiotoxicity in Male Rats. Global Journal of Medical research Interdsciplinary 13: 2249-4618.
 Beutler E, Duron O and Kelly, B.M (1963): Improved method for the determination
 Chen, D.; Huang, C.; Chen, Z.(2019): A review for the pharmacological effect of lycopine in central nervous system disorders. Biomed. Pharmacother. 111, 791â€“801. [CrossRef] https://doi.org/10.1016/j.biopha.2018.12.151.
 Crowe-White, K.M.; Phillips, T.A.; Ellis, A.C.(1019): Lycopine and cognitive function. J. Nutr. Sci. 8. [CrossRef] https://doi.org/10.1017/jns.2019.16.
 Dasari, S. and Tchounwou PB.(2014): Cisplatin in cancer therapy: Molecular mechanisms of action. Eur J Pharmacol 740:364-78. https://doi.org/10.1016/j.ejphar.2014.07.025.
 Deng J, Kohda Y, Chiao H, Wang Y, Hu X, Hewitt SM, Miyaji T, McLeroy P, Nibhanupudy B, Li S, Star RA (2001): Interleukin-10 inhibits ischemic and Cisplatin-induced acute renal injury. Kidney Int 60(6):2118â€“2128. https://doi.org/10.1046/j.1523-1755.2001.00043.x.
 Dhillon, P., Amir, E., Lo, M., Kitchlu, A., Chan, C., Cochlin, S., Yip, P., Chen, E. and Lee, R.,Ng, P., (2019): A case-control study analyzing mannitol dosing for prevention of Cisplatin-induced acute nephrotoxiciy. J. Oncol. Pharm. Pract. : official publication of the International Society of Oncology Pharmacy Practitioners 25, 875â€“883. https://doi.org/10.1177/1078155218771461.
 El-Shaimaa, A., Arafa , Wafaa, R., Mohamed, Dana, M., Zaher, Hany ,A. ,Omar .2020.Gliclazide attenuates acetic acid-induced colitis via the modulation of PPARÎ³, NF-ÎºB and MAPK signaling pathways . Toxicology and Applied Pharmacology, 391 , 114919. https://doi.org/10.1016/j.taap.2020.114919.
 Faten I. E.;Fatma, E.; Rania M. W., Mona A. E. (2021): Protective effect of Moringa olifera seed extract on Cisplatin induced nephrotoxicity in rats Int. J. Pharm Pharm. Sci, Vol 13, Issue 5, 78-82. https://doi.org/10.22159/ijpps.2021v13i5.41125.
 Gaskell H, Ge Xand Nieto N.(2018): High-mobility group box-1 and liver disease. Hepatol Commun. 2(9):1005â€“1020. https://doi.org/10.1002/hep4.1223.
 Gedik, S., Erdemli, M.E., Gul, M., Yigitcan, B., Gozukara Bag, H., Aksungur, Z., Altinoz, E.,(2017):. Hepatoprotective effects of crocin on biochemical and histopathological alterations following acrylamide-induced liver injury in Wistar rats. Biomed.Pharmaco.. 95, 764â€“770. https://doi.org/10.1016/j.biopha.2017.08.139.
 Hanan M. H. Lamya H Al-Wahaibi, Mohammed A . Yasmen F. M.(2020): Suppression of Cisplatin-Induced Hepatic Injury in Rats Through Alarmin High-Mobility Group Box-1 Pathway by Ganoderma lucidum: Theoretical and Experimental Study Drug Design, Development and Therapy 2020:14 2335â€“2353 https://doi.org/10.2147/DDDT.S249093.
 Jiang W, Guo MH, Hai X. (2016): Hepatoprotective and antioxidant effects of lycopene on non-alcoholic fatty liver disease in rat. World Gastroenterol 2016; 22(46): 10180-10188 [PMID: 28028366 https://doi.org/10.3748/wjg.v22.i46.10180.
 Jiang W., MEI-HUA G.and XIN, H., (2016).: Hepatoprotective and antioxidant effects of lycopine on non-alcoholic fatty liver disease in rat, World J. Gastroenterol., 22 (46): 10180-8. https://doi.org/10.3748/wjg.v22.i46.10180.
 Joshi, B.; Kar, S.K.; Yadav, P.K.; Yadav, S.; Shrestha, L.; Bera, T.K. (2020): Therapeutic and medicinal uses of lycopine: A systematic review. Int. J. Res. Med Sci. 2020, 8, 1195. [CrossRef] https://doi.org/10.18203/2320-6012.ijrms20200804.
 Koppelman B, Neefjes JJ, de Vries JE, de Waal MR. (1997) Interleukin-10 down-regulates MHC class II alpha beta peptide complexes at the plasma membrane of monocytes by affecting arrival and recycling. Immunity. 1997;7(6):861â€“871. ().https://doi.org/10.1016/S1074-7613(00)80404-5.
 Koracevic, D.; Koracevic, G.; Djordjevic V, Andrejevic, S and Cosic V (2001) Method for the measurement of antioxidant activity in human fluids.J Clin Pathol 54:356â€“361. https://doi.org/10.1136/jcp.54.5.356.
 Liu A, Pajkovic N, Pang Y, Zhu D, Calamini B, Mesecar Al, van Breemen RB (2006) Absorption and subcellular localization of lycopine in human prostate cancer cells. Mol Cancer Ther 5:2879â€“2885. https://doi.org/10.1158/1535-7163.MCT-06-0373.
 LourenÃ§o, S.C.; MoldÃ£o-Martins, M.; Alves, V.D. (2019):Antioxidants of natural plant origins: From sources to food industry applications. Molecules 24, 4132. [CrossRef] https://doi.org/10.3390/molecules24224132.
 Miller, R.P.;Tadagavadi RK, Ramesh G, Reeves WB (2010) Mechanisms of Cisplatin nephrotoxicity. Toxins (Basel) 2(11):2490â€“2518. https://doi.org/10.3390/toxins2112490.
 Muzandu K, El Bohi K, Shaban Z, Ishizuka M, Kazusaka A, Fujika S (2005) Lycopine and beta-carotene ameliorate catechol estrogen-mediated DNA damage. Jpn J Vet Res 52:173â€“184.
 Muzandu K, Ishizuka M, Sakamoto KQ, Shaban Z, El Bohi K, Kazusaka A, Fujita S (2006) Effect of lycopine and betacarotene on peroxynitrite-mediated cellular modifications. Toxicol Appl Pharmacol 215:330â€“340 https://doi.org/10.1016/j.taap.2006.03.006.
 Nagla A E. and Basma E. (2017): Proanthocyanidin protects against Cisplatin-induced oxidative liver damage through inhibition of inflammation and NF-jb/TLR-4 pathway. Environmental Toxico. 32:1952â€“1963. https://doi.org/10.1002/tox.22418.
 Narayanan KB., Ali M, Barclay BJ, Cheng Q, Dâ€™Abronzo L, Dornetshuber-Fleiss R and Park HH (2015): Disruptive environmental chemicals and cellular mechanisms that confer resistance to cell death, Carcinogenesis, 36: 89â€“ 110. https://doi.org/10.1093/carcin/bgv032.
 of blood glutathione. J Lab Clin Med 61:882â€“888.
 Ohkawa H, Ohishi N, Yagi K.(1979): Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8. https://doi.org/10.1016/0003-2697(79)90738-3.
 Omar HA, Mohamed WR, Arab HH, Arafa el SA. (2016) Tangeretin alleviates Cisplatin-induced acute hepatic injury in rats: targeting MAPKs and apoptosis. PLoS One.;11(3):e0151649. 2014; 66 (6):1050â€“1059. https://doi.org/10.1371/journal.pone.0151649.
 Przybylska, S.(2020): Lycopineâ€“a bioactive carotenoid offering multiple health benefits: A review. Int. J. Food Sci. Technol. 2020, 55, 11â€“32. https://doi.org/10.1111/ijfs.14260.
 Rabab F. H., M.D. and Fatma F. H., M.D (2019): Ameliorative Effect of Lycopine on Acrylamide-Induced Hepatotoxicity in Adult Albino Rats. Med. J. Cairo Univ., Vol. 87, No. 7, December: 4129-41 35. https://doi.org/10.21608/mjcu.2019.76710.
 Rjeibia, I. , Anouar , F.,b , Anouar B.: Sana N.c , Jazia S. , Saidia,I. , Souida,S. , Najla H. , Allaguib.M.S.(2017): Phytochemical characterization and bioactivity of Lycopineium europaeum: A focus on antioxidant, antinociceptive, hepatoprotective and nephroprotective effects. Biomed.& Pharmaco. 95 1441â€“1450 https://doi.org/10.1016/j.biopha.2017.09.035.
 Safety Data Sheet (2002): Sodium metabisulphite. Issued 25.09.2002. pp 1-3 Revision No. 1.
 SÃ¶nmez M, TÃ¼rk G, Ã‡eribaÅŸÄ± AO, Sakin F, and AteÅŸÅŸahin A (2011): Attenuating effect of lycopine and ellagic acid on 2, 3, 7, 8- tetrachlorodibenzo-p-dioxinâ€“induced spermiotoxicity and testicular apoptosis. Drug and Chemical Toxicology 34: 347-356 https://doi.org/10.3109/01480545.2011.557382.
 Urbonaviciene, D.; Viskelis, P. (2017): The Cisplatin-lycopine isomers composition in supercritical CO2 extracted tomato by-products. LWT-Food Sci. Technol. 85, 517â€“523. [CrossRef] https://doi.org/10.1016/j.lwt.2017.03.034.
 Veiga, M.; Costa, E.M.; Silva, S.; Pintado, M. (2020): Impact of plant extracts upon human health: A review. Crit. Rev. Food Sci. Nutr. 2020, 60, 873â€“886. https://doi.org/10.1080/10408398.2018.1540969.
 Waseem M, Bhardwaj M, Tabassum H, Raisuddin S, Parvez S. (2015) Cisplatin hepatotoxicity mediated by mitochondrial stress. Drug Chem Toxicol. 2015; 38: 452â€“459. https://doi.org/10.3109/01480545.2014.992437.
 Yilmaz HR, Iraz M, Sogut S, Ozyurt H, Yildirim Z, Akyol O(2004): The effects of erdosteine on the activities of some metabolic enzymes during Cisplatin-induced nephrotoxicity in rats. Pharmacol Res 50:287-90 https://doi.org/10.1016/j.phrs.2004.03.003.
 Yin, Y.; Zheng, Z.; Jiang, Z.(2019): Effects of lycopine on metabolism of glycopineolipid in type 2 diabetic rats. Biomed. Pharmacother. 2019, 109, 2070â€“2077. [CrossRef] [PubMed] https://doi.org/10.1016/j.biopha.2018.07.100.
 Yousef MI, Saad AA,and El-Shennawy LK. (2009): Protective effect of grape seed proanthocyanidin extract against oxidative stress induced by Cisplatin rats. Food Chem Toxicol. 47(6): 1176â€“1183 https://doi.org/10.1016/j.fct.2009.02.007.
 Zhao FJ, Ma JF, Meharg A.A Grath SP. (2009): Arsenic uptake and metabolism in plants. New Phytol 181:777â€“94. https://doi.org/10.1111/j.1469-8137.2008.02716.x.