Isolation of Streptococcus species from throat swabs of school going children

Authors

  • Shobha K.L Department of Microbiology Melaka Manipal Medical College Manipal Campus Manipal University Manipal
  • Kusumakshi Mrs Assistant Professor Department of Medical Laboratory Technology MSOAHS Manipal University,Manipal 576104
  • Anand K.M Selection grade lecturer Department of Microbiology Melaka Manipal Medical College(Manipal Campus) Manipal University,MANIPAL,576104
  • Gowrish S Selection grade lecturer Department of Microbiology Melaka Manipal Medical College(Manipal Campus) Manipal University,MANIPAL,576104
  • Jessica D'Souza Senior grade lecturer Department of Microbiology Melaka Manipal Medical College(Manipal Campus) Manipal University,MANIPAL,576104
  • Ramachandra L Professor Department of Surgery Kasturba Medical College,Manipal Manipal University Manipal, 576104

DOI:

https://doi.org/10.14419/ijpt.v2i2.3303

Published:

2014-09-12

Abstract

Introduction : Group A Streptococcus (Streptococcus pyogenes), is a Gram positive coccus appearing in chains and causes an incredible history of changing disease pattern. It has numerous virulence factors that helps in the adherence of the tissues, destruction of tissues and resulting in causing autoimmune complications. An attempt was made to screen for Streptococcus pyogenes throat carriers among school going children of Udupi district, Karnataka State, India.

Materials and Methods: Throat swabs were collected from posterior pharynx. Swabs were immediately plated onto a sheep blood agar (Hi-Media,Mumbai,India) and transported to the laboratory within one hour of collection. The inoculated plates were incubated at 370C for 24- 48 hours in carbondioxide incubator. Organism grown on colonies were identified up to species level.

Result: A total of 235 children were screened for Streptococcus pyogenes. They were in the age group of 6- 11 years. None of the children had Streptococcus pyogenes.

Conclusion: Streptococcus pyogenes was not found from the throat samples in school going children More studies are required to establish an accurate value of prevalence with a larger sample size.

Key words: Gram positive cocci in chains, Streptococcus pyogenes, school going children, throat swab

References

Cunningham MW. Pathogenesis of group A streptococcal infections. Clin Microbiol Rev. 2000; 13:470–511. http://dx.doi.org/10.1128/CMR.13.3.470-511.2000.

Heath A, Rita VJ, Barg NL, Engleberg NC. A two component regulatory system, CsrR-CsrS, represses expression of three Streptococcus pyogenes virulence factors, hyaluronic acid capsule, slo and pyrogenic exotoxin B. Infect Immun. 1999; 67:5298–305.

Severin A, Nickbarg E, Wooters J, Quazi SA, Matsuka YV, Murphy E, et al. Proteomic analysis and identification of Streptococcus pyogenes surface-associated proteins. J Bacteriol. 2007; 189:1514–22 http://dx.doi.org/10.1128/JB.01132-06.

McIver KS, Heath AS, Scott JR. Regulation of virulence by environmental signals in group a streptococci: influence of osmolarity, temperature, gas exchange and iron limitation of emm transcription. Infect Immun. 1995;63:4540–2

Chaussee MA, Dmitriev AV, Callegari EA, Chaussee MS. Growth phase-associated changes in the transcriptome and proteome of Streptococcus pyogenes. Arch Microbiol. 2008; 189:27–41 http://dx.doi.org/10.1007/s00203-007-0290-1.

H. Nsanze, K. Dawson, A.S. Ameen and N. Mustafa. Group A streptococcal antigen detection in schoolchildren; Eastern Mediterranean Health Journal, Volume 4 issue 8, 1998, Page 260-264.

Bacitracin (taxo a) disk for beta-hemolytic streptococcus, WEBCLS procedure manual; http://webcls.utmb.edu/samplecourses/bact/Bact/procedures/bacitracindiskgrpastrep.htm.2010.

Dawson KP et al. The prevalence of group a streptococcal throat carriage in Al-Ain, United Arab Emirates. Annals of tropical pediatrics, 1996, 16:123-7.

Henry D Isenberg, Clinical Microbiology procedure handbook; 2010; ASM press. http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Gram_Stain/Gram_stain.htm.

Koneman's color atlas and textbook of diagnostic microbiology;Lippincott Williams.2006; 6th Edition;674-688.

Thangam Menon, S. Shanmugasundaram*, M. Palani Kumar & C.P. Girish Kumar; Group A streptococcal infections of the pharynx in a rural population in south India, Indian J Med Res 119 (Suppl) May 2004, pp 171-173.

Axana Haggar,Andreas Nerlich,Rajesh Kumar,Vinod JAbraham,Kootallur N Brahmadathan,Pallab Ray,Vanita Dhanda,John Melbin Jose Joshua,Narinder Mehra,Rene Bergmann,G.Singh Chhatwal and Anna Norrby-Teglund;Clinical and Microbiologic characteristics of invasive Streptococcus pyogenes in North and South India; Journal of Clin Microbiol.May 2012,Vol 50,no5 1626-1631 http://dx.doi.org/10.1128/JCM.06697-11.

R.Kumar,H.Vohra,A.Chakraborty,Y.P.Sharma,S.Bandhopadhya,V.Dhanda,V.Sagar, M.Sharma,B.Shah and N.K Ganguly; Epidemiology of group A Streptococcal pharyngitis and impetigo; A cross-sectional and follow up study in a rural community of Northern India; Indian Journal Of Medical research; 130,December 2009,765- 771.

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