In vivo study of Fagonia Cretica Linn toxicity in Sudan

  • Authors

    • Omer Abbas shendi university-shendi-sudan
    • Omer Izzeldin omdurman islamic university-omdurman-sudan
    • Rashid Abdalla shendi university-shendi-sudan
    2014-10-25
    https://doi.org/10.14419/ijpt.v2i2.3594

  • Abstract

    Purpose: The study targeted to determine the toxicological effects of ethanolic extracts of Fagonia cretica linn plant in Khartoum, Omdurman, and Shendi towns in Sudan during (January – February) 2013.

    Objectives: To determine the LD50 of the plant and to study the effects of the plant on the body weight, on vital organ weights, and anesthesia induction time effects.

    Methods: Different methods were adopted to achieve the objectives of this study, including Harborne methods for extraction. A total of (30), young adult Wistar rats, weighing (42.6 – 72.2) grams, fed standard rat food and water d libitum, were maintained at standard laboratory conditions. All rats were grouped into (3) groups, survived through the experiment by using the recommended progression doses of Fagonia cretica as (175, 550, 1750, 5000, 5000, & 5000) mg/kg/body weight screen shot of AOT425 statistical program of main test. Toxicological studies in vivo (LD50 determination) were done in all (3) groups of animals survive till the experiment was over after the administration of a selected high dose (5000) mg/kg/body weight. Ethical approval had been obtained from Omdurman Islamic University, Faculty of Pharmacy, and Department of Pharmacology under the number-(01/0/2013).

    Results: The LD50 is greater than 5000 mg/kg, indicates the high safety of the plant. The ethanolic extract of Fagonia cretica in doses of (100 & 300 mg/kg/body weight) has no effects on the rats relative vital organ weight (heart, liver, kidneys, lung and spleen) during the study period of (14) days compared to the control group whilst the dose of (600) mg/kg/body weight change the liver somewhat from the relevant control, although, has no effects on the rats body weight during the study period of (14) days compared to the control group monitored at day (0, 7, 14). The anesthesia induction time on rats, shows that the ethanolic extracts of the plant has neurological effect on rats.

    Conclusion: The toxicological determination of LD50 was found to be greater than (5000) mg/Kg, estimated by the special statistical software with three stopping criteria based on long term outcomes. This is indicating wide safety margin of Fagonia ethanolic extract which encourages its use by humans for the treatment of many diseases..

    Keywords: Fagonia Cretica Linn, Umm Showaika, Ethanolic Extraction, Toxicity.

  • References

    1. Paglia DE, Valentine WN: Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin. Med 1967, 70(1):158-169.
    2. Saied MA. Hamdard Pharmacopoeia of Easter Medicine. Hamdard. Academy, Karachi, Pakistan; 1969 phytochemical and biological activities of fagonia indica, p. 41–43.
    3. Titz F, Enzymatic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues. Anal Biochem 1969, 27(3):502-522. http://dx.doi.org/10.1016/0003-2697 (69)90064-5.
    4. Chopra, R.M., Handa, K, L; Kapur, L, D., and Chopra, I.C., Indigenous Drugs of India. 2nd ed. India, Academic Press, New Delhi 1982, p. 507.
    5. J.B.Harborne. phytochemical methods a guide to modern techniques of plant analysis, Second Edition, Chapman and Hall, London, 1984, P. 4-7.
    6. Taylor CP, Weber ML: Effect of temperature on synaptic function after reduced oxygen and glucose in hippocampal slices. Neuroscience 1993, 52(3):555-562. http://dx.doi.org/10.1016/0306-4522 (93)90405-5.
    7. Gamal, E.B.ELGhazali, Mahgoub, S.ELToham. Awatif, A.B.ELEgami. Waiel. S.Abdalla, Medicinal Plants of the Sudan, 1994, National Centre for Research, Medicinal & Aromatic Plants Research Institute, Khartoum, 52-53.
    8. Crack PJ, Taylor JM, de Haan JB, Kola I, Hertzog P, and Iannello RC: Glutathione peroxidase-1 contributes to the neuroprotection seen in the superoxide dismutase-1 transgenic, journal of blood flow and metabolism. 2003, vol 23(1), page 19-22.
    9. Avinash K Rawal, Manohar G Muddeshwar and Saibal K Biswas,Rubia cordifolia, Fagonia cretica linn and Tinospora cordifolia exert neuroprotection by modulating the,antioxidant system in rat hippocampal slices subjected to oxygen glucose deprivation, BMC Complement Altern Me. 2004, 4:11. 10.1186/1472-6882-4-11. http://dx.doi.org/10.1186/1472-6882-4-11.
    10. Sarfraz Khan Marwat, Mir Ajab Khan, Mushtaq Ahmad, Muhammad Zafarand Fazal-ur Sarhad Ethnophytomedicines for treatment of various diseases in D. I. Khan district Int J Pharm Biomed Sci 2010.
    11. Matthew Lam Cytotoxic activity of Fagonia cretica against human breast cancer cells Doctor of Philosophy Aston University. April 2012.

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  • How to Cite

    Abbas, O., Izzeldin, O., & Abdalla, R. (2014). In vivo study of Fagonia Cretica Linn toxicity in Sudan. International Journal of Pharmacology and Toxicology, 2(2), 104-106. https://doi.org/10.14419/ijpt.v2i2.3594

    Received date: 2014-09-14

    Accepted date: 2014-10-12

    Published date: 2014-10-25