Phytochemical evaluation and exploration of the hepatoprotective activity of 5 different formulations in CCL4 induced albino RATS

 
 
 
  • Abstract
  • Keywords
  • References
  • PDF
  • Abstract


    Background: Hepatotoxicity and Liver disorders are chronic disorders due to different causes. It affects people in their prime of life, predominantly between the ages of 25-75 years with unpredictable courses. The different formulations are assumed to have significant activity in the treatment of the Liver disorders.

    Objective: The present study planned to evaluate the synergistic efficacy activity of the different formulations using CCL4 induced hepatotoxic model albino rats.

    Materials & Methods: The Phytochemical analysis of the T.cordifolia and five different formulations were performed. The animals were divided into eight different groups of 6 animals each as CCl4 treated, Single Plant extracts treated and another different test drug treated groups except 1st group, which was treated with only normal saline. The drugs were administered orally, twice a day and continued for 20 days. On the last day, all the group of animals were treated with the 1ml/kg CCl4 I.P. The Statistical significance was assessed using One-way ANOVA.

    Results: It was observed that 5 different formulations, i.e. Herbolive Syrup, Hepatonej Syrup, Hepanej Capsule, Herbolive Capsule, and Hepatonej Capsule produced significant hepatoprotective effect on 21st day. All the Formulations have significantly reduced the elevated level of Total Bilirubin, Direct Bilirubin, SGPT, SGOT, and ALP level.

    Conclusion: The result reveals that all the Herbomineral formulations possess the better hepatoprotective activity compare to single T.cordifolia plant extract. It is due to synergistic action of the various plants and minerals used into the formulation which brings down the elevated liver damage parameter to almost normal level.


  • Keywords


    ALP; CCl4; SGOT; SGPT; Hepato Protective; Herbomineral Formulations.

  • References


      [1] Kumar V, Modi PK, Saxena KK (2013) Exploration of Hepatoprotective activity of aqueous extract of Tinospora cordifolia- An Experimental Study. Asian journal of Pharmaceutical and Clinical Research 6(1), 87-91.

      [2] Kalra S, Vithalani M, Gulati G, Kulkarni CM, Kadam Y, Pallivathukkal J, Das B, Sahay R, Modi KD (2013) Study of Prevalence of Non alcoholic Fatty Liver Disease (NAFLD) in Type-II Diabetes Patients in India (SPRINT). Journal of Association of Physicians of India 61, 448-453.

      [3] Chauhan LS (2014) Hepatitis in India: Burden, Strategies, and Plans. NCDC News Letter 3(1).

      [4] Ashour Ben FM, Alnagar FA, Treesh FA, Husien WMR, Alssaie MA, Alkholi MA (2015) Evaluation of the Protective Effects of Savliv Drops in CCL4- Induced Hepatic Fibrosis Albino Rats. Journal of Vaterinary Advances 4(9), 1105-1121. http://dx.doi.org/10.5455/jva.20150903025602.

      [5] Patel D, Panchal M, Mavani H, Shah DR, Joshi S, Vyas B (2013) Phytochemical Screening and Standardization of Polyherbal Formulation “RIPARE” for Arthritis. International journal of Pharma Research & Review 2(6), 29-35.

      [6] Verma S, Sharma H, Garg M (2014) Phyllanthus amarus: A Review. Journal of Pharmacognosy and Phytochemistry, 3(2), 18-22.

      [7] Chokotia LS, Vashistha P, Sironiya R, Matoli H (2013) Pharmacological Activities of Eclipta Alba (L.). International Journal of Research and Development in Pharmacy and Life Sciences 2(4), 499-502.

      [8] Gora RH, Baxla SL, Kerketta P, Patnaik S, Roy BK (2014) Hepatoprotective activity of Tephrosia purpure against arsenic induced toxicity in Rats. Indian Journal of Pharmacology 46(2), 197-200. http://dx.doi.org/10.4103/0253-7613.129317.

      [9] Venkatalakshmi P, Vallabi E, Netaji S (2011) Hepatoprotective Activity of Boerhavia diffusa against Paracetamol induced toxicity in rats. Journal of chemical and pharmaceutical research 3(6), 229-232.

      [10] Jarukamjorn K, Nemoto N (2008) Pharmacological aspects of Andrographis paniculata on health and its major diterpenoid constituents Andrographolide. Journal of health science 54(4), 370-81. http://dx.doi.org/10.1248/jhs.54.370.

      [11] Mir AI, Kumar B, Tasduq SA, Gupta DK, Bhardwaj S, Johri RK (2007) Reversal of Hepatotoxin-induced pre-fibrogenic events by Emblica officinalis- A histological study. Indian journal of Experimental Biology 45, 626-629.

      [12] Sultana N, Najam R (2012) Gross toxicities and hepatoprotective effect of Aloe vera (L) Burm.F. International research journal of Pharmacy 3(10), 106-110.

      [13] Pingale SS (2011) acute tocxicity study for Tinospora Cordifolia. International journal of Research in Ayurveda and Pharmacy 2(5), 1571- 1573.

      [14] Arka G, Kundu A, Seth A, Singh AK, Maurya SK (2015) Preliminary evaluation of hepatoprotective potential of the polyherbal formulation. Journal of Intercultural Ethnopharmacology 4(2), 118-124.

      [15] Osadebe PO, Okoye FB, Uzor PF, Nnamani NR, Adiele IE, Obiano NC (2012) Phytochemical analysis, hepatoprotective and antioxidant activity of Alchornea cordifolia methanol leaf extract on carbon tetrachloride-induced hepatic damage in rats. Asian Pac J Trop Med 5(4), 289-293. http://dx.doi.org/10.1016/S1995-7645(12)60041-8.

      [16] Gumucio JJ (1989) Hepatocyte heterogeneity: The coming of age from the description of a biological curiosity to a partial understanding of its physiological meaning and regulation. Hepatology 9, 154-60. http://dx.doi.org/10.1002/hep.1840090124.


 

View

Download

Article ID: 5962
 
DOI: 10.14419/ijpt.v4i1.5962




Copyright © 2012-2015 Science Publishing Corporation Inc. All rights reserved.