Comparative endocrine disrupting effects of abamectin and indoxacarb insecticides
Keywords:Insecticides, Avermectins, Oxadiazine, White Albino Rats, Endocrine Biomarkers.
Abamectin and indoxacarb are relatively new insecticides with different mode of action and are applied to control a wide range of plant pests. However, their side effects to mammals are not fully studied. Accordingly, current study aimed to compare the adverse effects of both insecticides against the endocrine biomarkers: triiodothyronine (T3), tetraiodothyronine (T4), follicular-stimulating hormone (FSH), progesterone, and testosterone. These parameters were measured after orally-injecting rats with 1/20 LD50 doses of each of abamectin and indoxacarb for 60 days. Results showed that indoxacarb had pronounced reduction in the contents of T3 and FSH hormones compared to control and abamectin. Also, indoxacarb increased testosterone level compared to abamectin. T4 level was reduced by abamectin treatment compared to indoxacarb. Progesterone content was significantly increased after the abamectin treatment, while it was decreased after the indoxacarb treatment. However, the tested insecticides belong to avermectins and oxadiazine chemical groups that have different mode of action. They showed some similarity in their effect on T3, T4, and FSH, except for the progesterone hormone that showed a contradicting response. These two insecticides were marked as safe but current study highlight the need for caution during their application in the integrated pest management program.
 Abd-Elhady, H.K. (2012). Insecticidal activity and chemical composition of essential oil from Artemisia judaica L. against Callosobruchus maculates (F.) (Coleoptera: Bruchidae). J Plant Prot Res 52: 347â€“352. http://dx.doi.org/10.2478/v10045-012-0057-9.
 Abd-Elhady, H.K. and G.E. Abou-Elghar (2013) Abamectin induced biochemical and histopathological changes in the albino rat, Rattus norvegicus. Journal of Plant Protection Research 53: 263-270. http://dx.doi.org/10.2478/jppr-2013-0039.
 Abd-Elhady, H.K. and H.M. Heikal (2011) Selective toxicity of three acaricides to the two-spotted spider mite Tetranychus urticae and predatory mite Phytoseuilus persimilis in apple orchards. J Entomol 8: 574â€“580. http://dx.doi.org/10.3923/je.2011.574.580.
 BASF (2013). Insecticide Mode of Action. Technical Training Manual. Crop Protection Division. Global Strategic Marketing, Insecticides. BASF Corporation. GL-8006A December 2013. www.agro.basf.com
 Burke, C.W. and C.J. Eastman (1974) Thyroid hormones. Brit Med Bull 30: 93-99.
 Cameron, E.D.H. and J.J. Scarisbrik (1973) Radioimmuno assay of plasma progesterone. Clin Chem 19: 1403-1408.
 Celik-Ozenci, C., A. Tasatargil, M. Tekcan, L. Sati, E. Gungor, M. Isbir, and R. Demir (2011) Effects of abamectin exposure on male fertility in rats: Potential role of oxidative stress-mediated poly (ADP-ribose) polymerase (PARP) activation. Regulatory Toxicology and Pharmacology 61: 310â€“317. http://dx.doi.org/10.1016/j.yrtph.2011.09.001.
 Clark, J.M., J.G. Scott, F. Campos, and J.R. Bloomquist (1995) Resistance to avermectins: extent, mechanisms, and management implications. Annu Rev Entomol 40: 1â€“30. http://dx.doi.org/10.1146/annurev.en.40.010195.000245.
 Dinter, A. and A.J. Wiles (2000) Safety of the new Dupont insecticide indoxacarb to beneficial arthropoda an overview. IOBC/Wp RS Bulletin 23: 149-156.
 Ermer, J. (2005) Performance parameters, calculations and tests. In: Method Validation in Pharmaceutical Analysis. A Guide to Best Practice, ed. J. Ermer and J.H. McB Miller. Weinheim: WilleyVCH Verlag GmbH & Co. KGaA. Ch 2. http://dx.doi.org/10.1002/3527604685.ch2.
 Granner, D.K. (2003) the Diversity of the Endocrine System. In: Harperâ€™s Illustrated Biochemistry. Twenty-sixth edition. R.K. Murray, D.K. Granner, P.A. Mayes, and V.W. Rodwell. Lange Medical Books/McGraw-Hill. Medical Publishing Division. Ch 42.
 Granoff, A.B. and G.E. Abraham (1979) Peripheral and adrenal venouse levels of steroids in a patient with virilizing adenoma. Obstet Gynecol 53: 111.
 IRAC (Insecticide Resistance Action Committee) (2016) IRAC Mode of Action Classification Scheme. Version 8.1. www.irac-online.org (April 2016).
 Kakabakos, S.E. and M.J. Khosravi (1992) direct time resolved florescence immunoassay of progesterone in serum involving the biotin streptavidin system and the immobilized antibody approach. Clin Chem 38: 725-730.
 Kirsten, D. (2000) the thyroid gland: physiology and pathophysiology. Neonatal network: J Neonat Nurs 19: 11. http://dx.doi.org/10.1891/0730-0818.104.22.168.
 Kolar, L., N.K. Erzen, L. Hogerwerf, and C.A.M. Van Gestel (2008) Toxicity of abamectin and doramectin to soil invertebrates. Environ Pollut 151: 182â€“189. http://dx.doi.org/10.1016/j.envpol.2007.02.011.
 Korystov, Y.N., V.A. Mosin, V.V. Shaposhnikova, M.K. Levitman, A.A. Kudryavtsev, E.B. Kruglyak, T.S. Sterlina, A.V. Viktorov, and V.A. Drinyaev (1999) A comparative study of effects of aversectin C, abamectin and ivermectin on apoptosis of rat thymocytes induced by radiation and dexamethasone. Acta Vet BRNO 68: 23â€“29. http://dx.doi.org/10.2754/avb199968010023.
 Langouche, L. and G. Van den Berghe (2008) The Dynamic Neuroendocrine Response to Critical Illness. In: Van den Berghe, G (Ed.). Contemporary Endocrinology: Acute Cause to Consequence. Humana Press, New York, http://dx.doi.org/10.1007/978-1-60327-177-6.
 Lankas, G.R. and L.R. Gordon (1989) Toxicology. In: Campbell, WC (Ed.), Ivermectin and Abamectin. Springer-Verlag, New York, pp. 10â€“142 http://dx.doi.org/10.1007/978-1-4612-3626-9_6.
 Liu, T.X., Jr. A.N. Sparks, W. Chen, G.M. Liang, and C. Brister (2002) Toxicity, persistence and efficacy of indoxacarb on cabbage looper (Lepidoptera: Noctuidae) on cabbage. Economic Entomol 92: 360-367. http://dx.doi.org/10.1603/0022-0493-95.2.360.
 Marshall, J.C. (1975) Clinic in Endocrinol Metab 4: 545. http://dx.doi.org/10.1016/S0300-595X(75)80047-8.
 McCavera, S., T.K. Walsh, and A.J. Wolstenholme (2007) Nematode ligand-gated chloride channels: an appraisal of their involvement in macrocyclic lactone resistance and prospects for developing molecular markers. Parasitology 134: 1111â€“1121. http://dx.doi.org/10.1017/S0031182007000042.
 Mebis, L., L. Langouche, and G. Van den Berghe (2008) Changes within the thyroid axis during the course of critical illness. In: Van den Berghe, G (Ed.). Contemporary Endocrinology: Acute Cause to Consequence. Humana Press, NY. http://dx.doi.org/10.1007/978-1-60327-177-6.
 Mudaraddi, T.Y., R.R. Potadar, and B.B. Kaliwal (2012) Indoxacarb induces liver oxidative stress in Swiss Albino Mice. European Journal of Experimental Biology 2: 180-186.
 National Research Council (2011) Guide for the Care and Use of Laboratory Animals, 8th ed.; the National Academies Press: Washington, DC, USA.
 PacquÃ©, M., B. MuÃ±oz, G. Poetschke, J. Foose, B.M. Greene, and H.R. Taylor (1990) Pregnancy outcome after inadvertent ivermectin treatment during community-based distribution. Lancet 336: 1486-1489. http://dx.doi.org/10.1016/0140-6736(90)93187-T.
 Puri, D. (2011) Textbook of Medical Biochemistry, 3rd Edition. Elsevier, A division of Reed Elsevier India Private Limited. ISBN 978-81-312-2312-3.
 Quraishi, R., F. Akbar, S. Karim, S. Ahmad, W. Rashid, and S. Ullah (2015) Effects of pesticides on haematology, Thyroid stimulating hormone (TSH) and tri-iodothyronine (T3) hormones of agricultural workers in Swat, Pakistan. J Biol Life Sci 6: 96-107.
 Rebar, R.W., G.F. Erickson, and S.S.C. Yen (1982) Fertil Steril 37: 35. http://dx.doi.org/10.1016/S0015-0282(16)45973-X.
 SAS (Statistical Analysis System) (Version 9.2) (2013) SAS Institute Inc., Cary, NC 27513-2414 USA.
 Seixas, J.N., P.V. Peixoto, A.G. ArmiÃ©n, F.F. Jabour, and M.F. Brito (2006) Clinical and pathogenetic aspects of abamectin poisoning in calves. Pesq Vet Bras 26: 161â€“166. http://dx.doi.org/10.1590/S0100-736X2006000300006.
 Shit, S.P., R.S Panghal, V. Kumar, and R.D. Rana (2008) Acute toxicity and gross behavioural effects of indoxacarb in laboratory animals. Haryana Vet 47: 49-51.
 Skelley, D., L. Brown, and P. Besch (1973) Radioimmunoassay. Clin Chem 19: 146.
 Tomlin, C.D.S. (Ed.) (2004) A world compendium. The e-Pesticide Manual. Thirteenâ€™s Edition. Version 3.0 2003-04. British Crop Protection Council (BCPC).
 Turner, M.J. and J.M. Schaeffer (1989) Mode of action of ivermectin. In: Campbell, WC (Ed.), Ivermectin and Abamectin. Springer-Verlag, New York, pp. 73â€“78. http://dx.doi.org/10.1007/978-1-4612-3626-9_5.
 Wing, K.D., M.E. Schnee, M. Sacher, and M. Connair (1998) A novel oxadiazine insecticide is bioactivated in lepidopteran larvae. Archive Insect Biochem Physiol 37: 91-103. http://dx.doi.org/10.1002/(SICI)1520-6327(1998)37:1<91::AID-ARCH11>3.0.CO;2-5.
 Yen, P.M. (2001) Physiological and molecular basis of thyroid hormone action. Physiol Rev 81: 1097â€“1142.