Ethanolic leaf extract of Irvingia gabonensis (o’Rorke) Baill protects against nephrotoxocity and hepatotoxicity in cadmium-induced wistar albino rats

  • Authors

    • Efosa Ewere University of Uyo, Uyo, Akwa Ibom State, Nigeria.
    • Samson Oyebadejo University of Uyo, Uyo, Akwa Ibom State, Nigeria.
    • Victor Peter University of Uyo, Uyo, Akwa Ibom State, Nigeria.
    2016-06-06
    https://doi.org/10.14419/ijpt.v4i2.6186
  • Irvingia Gabonensis (O’Rorke) Baill, Cadmium, Nephrotoxicity, Hepatotoxicity, Biomarkers.

  • Abstract

    Cadmium is a well-known environmental pollutant that has been proven to be nephrotoxic and hepatotoxic in the body. In this study, the effect of ethanolic leaf extract of Irvingia gabonensis (O’Rorke) Baill (IG) against cadmium-induced nephrotoxicity and hepatotoxicity in Wistar albino rats was investigated. 30 female wistar rats of weights between 98-153g were grouped into 6 groups of 5 animals each. Group 1 served as the control and was placed on rat feed and water. Groups 2, 3, and 4 were administered 10mg/Kg body weight (mg/kgbw) of cadmium chloride (CdCl2) only,10mg/Kgbw CdCl2 and 200mg/kgbw extract, 10mg/Kgbw CdCl2 and 400mg/kgbw extract, respectively. Groups 5 and 6 were given 200mg/kgbw and 400mg/kgbw of IG extract respectively and the treatments lasted for 28 days. Results obtained revealed significant (p<0.05) increases in the serum levels of all renal and liver function biomarkers in group 2 (CdCl2 only) as compared with the control. There were however significant (p<0.05) decreases in the serum levels of the assayed parameters when groups 3 and 4 were respectively compared with group 2. It can therefore be concluded that ethanolic leaf extract of Irvingia gabonensis (O’Rorke) Baill enhances the integrity of the kidneys and liver of cadmium-induced wistar albino rats.

  • References

    1. [1] Abd-El-Baset, M., and Abd El-reheem, A. (2008). “The roles of honeybee solution on the physiological parameters of rats exposed to cadmium chloride,†Australian Journal of Basic and Applied Sciences. 2: 1438–1453.

      [2] Alhazzi, JM. (2008). Cadmium induced Hepatotoxicity and oxidative stress in Rats: Protection by Selenium. Research Journal of Environmental Sciences 2(4): 305-309. http://dx.doi.org/10.3923/rjes.2008.305.309.

      [3] Alvarez, SM., Gómez, NN, Scardapane, L., Fornés, MW, and Giménez, M. S. (2007). “Effects of chronic exposure to cadmium on prostate lipids and morphology,†BioMetals, 20(5): 727–741. http://dx.doi.org/10.1007/s10534-006-9036-9.

      [4] Ambily, RN., Olivier, D., Karen, S., Emmy, VK. and Ann, C. (2013) Cadmium-Induced Pathologies: Where is the oxidative balance lost (or not)? International Journal of Molecuar Sciences. 14: 6116-6143. http://dx.doi.org/10.3390/ijms14036116.

      [5] Amin, A., Hamza, AA. Daoud, S. and Hamza, W. (2006) Spirulina Protects against Cadmium-induced Hepatotoxicity in Rats. American Journal of Pharmacology and Toxicology 1(2): 21-25. http://dx.doi.org/10.3844/ajptsp.2006.21.25.

      [6] Amoruso, MA. Witz, G. and Goldstein, BD. (1982) Enhancement of rat and human phagocyte superoxide anion radical production by cadmium in vitro. Toxicology Letters. 10:133-8. http://dx.doi.org/10.1016/0378-4274(82)90064-9.

      [7] Arvind, B., Anurag, B., and Shina, M. (2005) Approach to Renal Tubular Disorders. Indian Journal of Pediatrics 72 (9): 771-776. Doi: 10.1007/ BF02734150.

      [8] Asagba, SO. and Eriyamremu, GE., Adaikpoh, MA. and Ezeoma, A. (2004) Levels of lipid peroxidation, superoxide dismutase, Na+/K+- ATPase in some tissues of rats exposed to a Nigerian diet and cadmium. Biological Trace Element Research 100(1): 075-086. Doi: 10.1385/BTER.100:1:075

      [9] Bagchi, D., Vuchetich, P., Bagchi, M., Hassoun, EA, Tran, MX., Tnag, L. and Stohs, SJ. (1997) Induction of oxidative stress by chronic administration of sodium dichromate [chromium VI] and cadmium chloride [cadmium II] to rats. Free Radical Biology and Medicine 22:471-8. http://dx.doi.org/10.1016/S0891-5849(96)00352-8.

      [10] Betro, MG. Oon, RC. and Edwards, JB. (1973) Gamma-glutamyl transpeptidase and other liver function tests in myocardial infarction and heart failure. American Journal of Clinical Pathology 60(5): 679-683. PMID 4148050.http://dx.doi.org/10.1093/ajcp/60.5.679.

      [11] Bishop, M., Duben-Engelkirk, J. and Fody, E. (2000) Clinical chemistry: principles, procedures, correlations. 4th ed. Philadelphia (PA): Lippincott Williams and Wilkins.

      [12] Bodansky, O. (1995) Enzymes in cancer: The phosphohydrolases. In: Biochemistry of human cancer. Bodansky O. (Ed.), New York: Academic Press. 61-92.

      [13] Burtis, CA. Ashwood, ER. And Burns, DE. (2006) Tietz textbook of clinical chemistry and molecular diagnostics (4th ed.). New Delhi: Saunders.

      [14] Chawla, R. (2003) Practical Clinical Biochemistry: Methods and interpretations. New Delhi (India): Jaypee Brothers Publishers.

      [15] Cray, C., Zaias, J. and Altman, NH. (2009) acute phase response in animals: a review. Comparative Medicine. 6: 517-526.

      [16] Crook, MA. (2006) Clinical chemistry and metabolic medicine (7th ed.). New Delhi: Edward Arnold.

      [17] Crook, MA. (2007) the kidneys. In: Clinical chemistry and metabolic medicine (8th ed.). M.A. Crook (Ed.), Britian: Brookpower, Pp 36-57.

      [18] Cuypers, A., Plusquin, M., Remans, T., Jozefczak, M., Keunen, E., Gielen, H., Opdenakker, K., Nair, A., Munters, E., Artois, T., Nawrot, T., Vangronsveid, J., and Smeets, K. (2010). Cadmium stress: An oxidative challenge. Biometals. 23: 927–940. http://dx.doi.org/10.1007/s10534-010-9329-x.

      [19] Eneman, JD, Potts, RJ, Osier, M., Shukla, GS, Lee, CH.Chin, JF. and Hart, BA. (2000) Suppressed oxidant induced apoptosis in cadmium adapted alveolar epithelial cells and its potential involvement in cadmium carcinogenesis. Toxicology 7:215-28.

      [20] Ewere, EG, Uka, E and Usunobun, U. (2016). Phytochemical Composition, Invitro Antioxidant Activity and Acute Toxicity of Irvingia gabonensis (O’Rorke) Baill Ethanolic Leaf Extract. International Journal of Biological Research. 4 (1): 36-41.

      [21] Gbadegesin, MA, Adegoke, AM., Ewere, EG. and Odunola, OA. (2014). Hepatoprotective and anticlastogenic effects of ethanol extract of Irvingia gabonensis (IG) leaves in sodium arsenite-induced toxicity in male Wistar rats. Nigerian Journal of Physiological Sciences. 29: 029 –036.

      [22] Gonick, HC. (2008). Nephrotoxicity of cadmium & lead. Indian Journal of Medical Research. 128: 335-352.

      [23] Halim, A., El-Almady, O., Hassab-Allah, S., Abdel-Gelil, F., Hafez, Y. and Derivis, A. (1997) Biochemical effect of antioxidants on lipids and liver function in experimentally induced liver damage. Annals of Clinical Biochemistry. 34:656-663.http://dx.doi.org/10.1177/000456329703400610.

      [24] Heinegard. D. and Tiderstrom. G. (1973). Determination of serum creatinine by direct colorimetric method. Clinica Chimica Acta .43(3):305-310. http://dx.doi.org/10.1016/0009-8981(73)90466-X.

      [25] Hubert DJ, Wabo FG, Ngameni B, Ngheguin TF, Tchoukoua A, Ambassa P, Abia W, Tchana AN, Giardina S, Buonocore D, Vita FP, Vidari G, Marzatico F, Ngadjui BT. and Moundipa PF. (2010) “In vitrohepatoprotective and antioxidant activities of the crude extract and isolated compounds from Irvingia gabonensis. Asian Journal of Traditional Medicine. 5(3): 79-88.

      [26] Huseby, NE. (1978) Multiple forms of gamma-glutamyltransferase in normal human liver, bile and serum. Biochimca et Biophysica Acta 522(2): 354-362. http://dx.doi.org/10.1016/0005-2744(78)90069-4.

      [27] Huseby, NE. (1982) Multiple forms of serum gamma-glutamyltransferase. Association of the enzyme with lipoproteins. Clinica Chimica Acta 124(1): 103-112. http://dx.doi.org/10.1016/0009-8981(82)90324-2.

      [28] Jaeschke, H., Gores, GJ, Cederbaum, AI, Hinson, JA, Pessayre, D. and Lemasters, JJ. (2002) Mechanisms of hepatotoxicity. Toxicological Sciences. 65:166–176. http://dx.doi.org/10.1093/toxsci/65.2.166.

      [29] Järup, L., Berglund, M., Elinder, C. G., Nordberg, G. and Vahter, M. (1998). Health effects of cadmium exposure -a review of the literature and a risk estimate. Scandinavian Journal of Work,Environment and Health. 24 (1):1–51. PMID: 9569444

      [30] Järup, L., Hellström, L., Alfvén, T., Carlsson, M., Grubb, A., Persson, B., Pettersson, C., Spang, G., Schutz, A., and Elinder, C. (2000). Low level exposure to cadmium and early kidney damage: the OSCAR study. Occupational and Environmental Medicine. 57 (10): 668-672. http://dx.doi.org/10.1136/oem.57.10.668.

      [31] Johannes, G., Franziska, S., Christian, G., Vera, E., Paul, B., Andrea, R., and David, A. G. (2006). The toxicity of cadmium and resulting hazards for human health. Journal of Occupational Medicine and Toxicology. 1: 22. http://dx.doi.org/10.1186/1745-6673-1-22.

      [32] Kaya, S., Pirincci I., Tras, B., Unsal, A., Bilgili, A, Akar, F., Dogan, A. and Yarsan, E. (2002) Metals, Other Inorganic vet Radioactive Agents. In: Toxicology in Veterinary Medicine, Kaya S I. Pirincci and A. Bilgili (Eds.). 2nd Edn, Medisan Press. Ankara. Pp. 207-250.

      [33] Kochmar, JF. and Moss, DW. (1974) Fundamentals of Clinical Chemistry, N.W. Tietz. 604.

      [34] Lakshmi, GD., Kumar, PR., Bharavi, K., Annapurna, P., Rajender, B., Patel, PT., Satish Kumar, CSV and Rao, GS. (2012) Protective effect of Tribulus terrestris linn on liver and kidney in cadmium intoxicated rats. Indian Journal of Experimental Biology. 50: 141-146.

      [35] Layachi, N. and Kechrid, Z. (2012) Combined protective effect of vitamins C and E on cadmium induced oxidative liver injury in rats. African Journal of Biotechnology. 11(93): 16013-16020. Doi: 10.5897/AJB12.2665

      [36] Liao, XY. Chen, TB, Xie, H. and Liu. YR. (2005). “Soil as contamination and its risk assessment in areas near the industrial districts of Chenzhou City, Southern China,†Environment International. 31(6): 791–798. http://dx.doi.org/10.1016/j.envint.2005.05.030.

      [37] Limei, Z., Xiaoyong, L., Tongbin, C., Xiulan, Y., Hua, X., Bin, W. and Lixia, W. (2008). “Regional assessment of cadmium pollution in agricultural lands and the potential health risk related to intensive mining activities: a case study in Chenzhou City, China,†Journal of Environmental Sciences. 20 (6):696–703. http://dx.doi.org/10.1016/S1001-0742(08)62115-4.

      [38] Liu, J., Qian, SY, Guo, Q., Jiang, J., Waalkes, MP, Mason, RP. and Kadiiska, M. (2008). Cadmium generates reactive oxygen- and carbon-centered radical species in rats: insights from in vivo spin-trapping studies. Free Radical Biology and Medicine. 45: 475–481. http://dx.doi.org/10.1016/j.freeradbiomed.2008.04.041.

      [39] Lowe AJ, Gillies ACM, Wilson J. and Dawson IK. (2000) Conservation genetics of Bush Mango from Central/West Africa: Implications for RAPD analysis. Molecular Ecology. 9(7): 831 – 841. http://dx.doi.org/10.1046/j.1365-294x.2000.00936.x.

      [40] Lum, G, Gambino, SR. (1972) Serum gamma glutamyltranspeptidase activity as an indicator of disease of liver, pancreas, or bone. Clinal Chemistry. 18(4):358-362. PMID: 5012259

      [41] Maruna, R. F. (1958). Clinical Chemistry. Acta, 2(1):581.

      [42] Matos L, Nzikou JM, Matouba E, Pandzou-Yembe VN, Mapepoulou TG, Linder M, Desobry S (2009). Studies of Irvingia gabonensis seeds kernels: Oil technological applications. Pakistan. Journal of Nutrition 8: 151-157. Doi: 10.3923.pjn.2009.151.157.

      [43] Murugavel, P., Pari, L. (2007) Effect of diallyl tetrasulfide on cadmium-induced oxidative damage in the liver ofrats. Human and experimental toxicology 26(6):527-34. http://dx.doi.org/10.1177/0960327107073810.

      [44] NAERLS (National Agricultural Extension and Research Liaison Services) (1999) Production and utilization of Ogbono (Irvingia gabonensis). Extension bulletin No 140. Horticulture series No 4. Pp. 3-12.

      [45] Noonan, CW., Sarasua, SM., Campagna, D., Kathman, SJ, Lybarger, JA. and Mueller, PW. (2002) Effects of exposure to low levels of environmental cadmium on renal biomarkers. Environmental Health Perspectives. 110(2): 151-155. http://dx.doi.org/10.1289/ehp.02110151.

      [46] Nosiri, C., Abdu-Aguye, I., Hussaini, I. & Abdurahaman, E. (2009a). Leaf Extracts of Irvingia gabonensis Increase Urine Output and Electrolytes in Rats. The Internet Journal of Alternative Medicine 8(2): http://ispub.com/IJAM/8/2/11789.

      [47] Nosiri, C., Hussaini, I., Abdu-Aguye, I. and Abdurahaman, E. (2009b). Pharmacological Effect of Irvingia gabonensis Leaf Extracts on Cat Blood Pressure. The Internet Journal of Pharmacology 9(1): http://ispub.com/IJPHARM/9/1/6633.

      [48] Nwankwo, E., Nwankwo, B. and Mubi, B. (2006) Prevalence of impaired kidney in hospitalized hypertensive patients in Maiduguri, Nigeria. Internet Journal of Internal Medicine. 6 (1): 1-10.

      [49] Ochi, T., Otsuka, F., Takahashi, K. and Oshawa. M. (1998) Glutathione and metallothioneins as cellular defense against cadmium toxicity in culture Chinese hamster cells. Chemico-biologiy interactions. 65:1-14. http://dx.doi.org/10.1016/0009-2797(88)90026-9.

      [50] Ojo, OA, Ajiboye, BO, Oyinloye, BE, Ojo, AB. And Olarewaju, OI. (2014) Protective effect of Irvingia gabonensis stem bark extract on cadmium-induced nephrotoxicity in rats. Interdiscplinary Toxicology. 7: 208-214. http://dx.doi.org/10.2478/intox-2014-0030.

      [51] Rajasekaran, A. and Periasamy, M. (2012) Hepatoprotective Effect of Ethanolic Leaf Extract of Calycopteris floribunda Lam on Cadmium-induced Hepatotoxicity in Rats. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 3(3): 382-390.

      [52] Thevenod, F. (2009). Cadmium and cellular signaling cascades: To be or not to be? Toxicology and Applied Pharmacology. 238: 221–239. Doi: 10.1016.j.taap.2009.01.013.

      [53] Thijssen, S., Cuypers, A., Maringwa, J., Smeets, K., Horemans, N., Lambrichts, I., and Van Kerkhove, E. (2007). Low cadmium exposure triggers a biphasic oxidative stress response in mice kidneys. Toxicology. 236(1-2): 29–41. http://dx.doi.org/10.1016/j.tox.2007.03.022.

      [54] Tietz, NW. (1976). Fundamentals of clinical chemistry. Philadelphia: W. B. Saunders.

      [55] Tobacco, A., Meiatini, F., Moda, E. and Tarli, P. (1979) Simplified enzymatic/colorometric serum urea nitrogen determination. Clinical Chemistry 25(2): 336-337. PMID: 759035

      [56] Trinder, P. (1951) A rapid method for the determination of sodium in serum. Analyst. 76:596-599. http://dx.doi.org/10.1039/an9517600596.

      [57] Udeala O, Onyechi J. and Agu S. (1980) Sourcing Pharmaceutical Raw Materials from Indigenous Medicinal plants. Journal of Pharmacy and Pharmacology. 32:36.

      [58] Udosen, EO, Ojong, AS. (1998) hepatotoxic activity of Sacoglothis gabonensis in rats. Pharmaceutical Biology. 36(5):467-469. http://dx.doi.org/10.1076/phbi.36.5.368.4648.

      [59] Vermuelen, NPE, Bessems, JGM, Van de straat, R. (1992) Molecular aspects of paraceutamol-induced hepatotoxity and its mechanism-based prevention. Drug Metabolism Reviews. 24(3): 367-407. http://dx.doi.org/10.3109/03602539208996298.

      [60] Wong, Z., Troll, W., Koenig, KL., Frenkel, K. (1990) Carcinogenic sulfide salts of nickel and cadmium-induced H2O2 formation by human polymorphonuclear leukocytes. Cancer Research. 20:7564-70.

      [61] Yamano, T., Kosanke, SD, and Rikans, LE. (1999) Attenuation of cadmium-induced liver injury in senescent male Fischer 344 rats: role of metallothionein and glutathione. Toxicology and Applied Pharmacology. 161(3): 225-230. http://dx.doi.org/10.1006/taap.1999.8813.

      [62] Yiin, SJ, Chern, CL., Sheu, JY., Tseng, WC., and Lin, TH. (1999) Cadmium-induced renal lipid peroxidation in rats and protection by selenium. Journal of Toxicology and Environmental Health. 57(6): 403-413. http://dx.doi.org/10.1080/009841099157601.

      [63] Young, DS., Davis, RB., O'Connor, BB. and Eisenberg, DM. (1975) Clinical Chemistry. 21:5

      [64] Young, DS. (1990) Effects of Drugs on clinical Laboratory Tests, 3rd edn., AACC Press. Washington D.C.

      [65] Zikic, RV., Stajn, A., Saicic, ZS., Spasic, MB., Ziemnicki, K. and Petrovic, VM. (1996) The activities of superoxide dismutase, catalase and ascorbic acid content in the liver of goldfish (Carassius auratus gibelio Bloch.) exposed to cadmium. Physiology Research 4596):479-481. PMID: 9085381.

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    Ewere, E., Oyebadejo, S., & Peter, V. (2016). Ethanolic leaf extract of Irvingia gabonensis (o’Rorke) Baill protects against nephrotoxocity and hepatotoxicity in cadmium-induced wistar albino rats. International Journal of Pharmacology and Toxicology, 4(2), 105-110. https://doi.org/10.14419/ijpt.v4i2.6186

    Received date: 2016-05-04

    Accepted date: 2016-05-30

    Published date: 2016-06-06