In vitro antioxidant potency of some smaller chain glycopeptides with the prediction of IC50 values

Authors

  • Kandasamy Nagarajan Professor & Head of Department, Department of Pharmaceutical Chemistry, Kiet School of Pharmacy, KIET Institute, Ghaziabad
  • Parul Grover Assistant Professor, KIET School of Pharmacy, KiET Institute, Ghaziabad
  • Roma Ghai Assistant Professor, KIET School of Pharmacy, KiET Institute, Ghaziabad
  • Ayushi Teharia Graduate Student, KIET School of Pharmacy, KiET Institute, Ghaziabad
  • Sanjeev Chauhan Assistant Professor, KIET School of Pharmacy, KiET Institute, Ghaziabad
  • Jagannath Sahoo Principal, KIET School of Pharmacy, KiET Institute, Ghaziabad

DOI:

https://doi.org/10.14419/ijpt.v4i2.6298

Published:

2016-07-19

Keywords:

Glycopeptides, Docking, Antioxidant Assay, ROS, Oxidative Stress.

Abstract

Hydrogen peroxide, 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) and Phosphomolybdenum in-vitro assay was employed to determine the antioxidant potency of glycopeptides RN Mannose, RK starch, RNRN Mannose and RHRCR Starch using ascorbic acid as the standard drug. The percentage scavenging activity of the glycopeptides were determined at different concentrations and the IC50 value of the test compounds were subsequently compared with that of ascorbic acid. RN Mannose was found to be most potent antioxidant compound. Also, Swiss dock study was performed with three glycopeptides, viz., RHRCR Mannose, RN Mannose and RNRN Mannose.Among these, RHRCR Mannose was found to have the best affinity for the receptor with stearic energy -0.2306kcal/mol.

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