Effects of food-chain mediated metal exposures on phosphatases’ profile in rats

Authors

  • Richard Ovie Delta State University, Abraka, Nigeria.
  • Samuel Asagba delta state university, abraka

DOI:

https://doi.org/10.14419/ijpt.v4i2.6362

Published:

2016-08-26

Keywords:

Arsenic, Cadmium, Exposures, Food-Chain, Rats.

Abstract

This study seeks at investigating the separate and combined toxicities of Arsenic (As) and Cadmium (Cd) administered along food-chain. The natural food-chain was mimicked by exposing rats to diet containing milled Cd and As-exposed (at a dose of 0.4mg/100ml each) catfish as source of protein. Control groups were fed with normal formulated rat feed with non-metal contaminated fish as source of protein. The effects of Cd and As exposures were sought by determining indices of plasma and lysosomal membrane integrity in plasma and organs of the rats after three months exposure time. The results depict that Cd and As exposures in the rats led to bioaccumulation of the metals in their plasma and organs. The results gotten revealed significance (p<0.05) in gain of body weight in both Cd and As exposed rats when control is compared to them. Phosphatases’ activities (ALP and ACP) in the plasma and vital organs were also significant in the metal inoculated rats when the control is compared to them. These results explained that arsenic and cadmium forms free radicals which cause stress due to oxidation, kidney impairment and immunologic disorders.

References

[1] Akanji MA, Olagoke OA & Oloyede OR (1993) Effect of chronic consumption of metabisulphite on the integrity of the kidney cellular system. Toxicology 81,173-179. http://dx.doi.org/10.1016/0300-483X(93)90010-P.

[2] Bahemuka TE & Mobufu EB (1999) Heavy metals in edible green vegetables grown along the site of the Simza and Msimbazi River in Dar es Salam in Tanzania. Food Chemistry 66, 63-66. http://dx.doi.org/10.1016/S0308-8146(98)00213-1.

[3] Braun M, Waheed a & Von Figura K (1989) Lysosomal acid phosphatase is transported to lysosomes via the cell surface. EMBO Journal 8, 12, 3633 – 3640.

[4] Butterworth PJ & Moss DW (1966) Action of neuraminidase on human kidney alkaline phosphatase. Nature 209, 805-810. http://dx.doi.org/10.1038/209805a0.

[5] Cheng TC (1988) In vitro effects of heavy metals on cellular defense mechanisms of C. virginica: phagocytic and endocytic indices. Journal of Invertebrate Pathology 51, 215-220. http://dx.doi.org/10.1016/0022-2011(88)90028-6.

[6] Cohen SM, Arnold LL, Eldan M, Lewis AS & Beck BD (2006) Methylated arsenicals: the implications of metabolism and carcinogenicity studies in rodents to human risk assessment. Critical Review in Toxicology 36, 99–133. http://dx.doi.org/10.1080/10408440500534230.

[7] Grundy MM, Moore MN, Howell SM & Ratcliffe NA (1996) Phagocytic reduction and effects on lysosomal membranes by polycyclic aromatic hydrocarbons, in haemocytes of Mytilus edulis. Aquatic Toxicology 34, 273-90. http://dx.doi.org/10.1016/0166-445X(95)00044-5.

[8] Hillman GZ (1971) Clinical biochemistry. Clinical Chemistry 3, 273.

[9] Hongyu L, Anne P & Bohan L (2005) Metal contamination of soils and crop affected by the Cehenzhou lead/ zinc mine spill (Hunan, China). Science of the Total Environment 339, 153-166. http://dx.doi.org/10.1016/j.scitotenv.2004.07.030.

[10] Hughes MF (2002) Arsenic toxicity and potential mechanisms of action. Toxicology Letters 133, 1–16. http://dx.doi.org/10.1016/S0378-4274(02)00084-X.

[11] Ikeda M, Zhang ZW, Shimbo S, Watanabe T, Nakatsu H & Moon CS (2000) Urban population exposure to lead (Pb) and cadmium in East and South East Asia. Science of the Total Environment 249, 373-384. http://dx.doi.org/10.1016/S0048-9697(99)00527-6.

[12] Iyengar V & Nair P (2000) Global outlook on nutrition and the environment meeting the challenges of the next millennium. Science of the Total Environment 249, 331-346. http://dx.doi.org/10.1016/S0048-9697(99)00529-X.

[13] Komarnicki GJK (2000) Tissue, sex and age specific accumulation of heavy metals (Zn, Cu, Pb, Cd) by populations of the mole (Talpa europaea L.) in a central urban area. Chemosphere 41, 10, 1593-1602. http://dx.doi.org/10.1016/S0045-6535(00)00018-7.

[14] Lacatus R, Rauta C, Carstea S & Ghelase I (1996) Soil- plant- man relationship in heavy metal polluted rear in Romania. Applied Geochemistry 11, 105-107. http://dx.doi.org/10.1016/0883-2927(95)00101-8.

[15] Liu SL, Jiang XL, Hu XK, Gong J, Hwang H & Mai KS (2004) Effects of temperature on non-specific immune parameters in two scallop species: Argopecten irradians (Lamarck 1819) and Chlamys farreri (Jones & Preston 1904). Aquaculture Research

[16] Mah V & Jalilehvand F (2010) Cadmium (II) complex formation with glutathione. Journal of Biology and Inorganic Chemistry 15, 441–458. http://dx.doi.org/10.1007/s00775-009-0616-3.

[17] Monteiro LR, Costa V, Furness RW & Santos RS (1996) Mercury concentrations in prey fish indicate enhanced bioaccumulation in mesopelagic environments. Marine Ecology Progress Series 141, 21-25. http://dx.doi.org/10.3354/meps141021.

[18] Mu HJ, Jiang XL, Liu SQ & Guan HS (1999) Effects of immunopolysaccharide on the activities of acid phosphatase, alkaline phosphatase and superoxide dismutase in Chlamys farreri. Journal of Ocean University, Qingdao 29,463-468.

[19] Nabi AHM, Rahman MM & Islam LN (2005) Evaluation of Biochemical Changes in Chronic Arsenic Poisoning among Bangladeshi Patients. International Journal of Environmental Research and Public Health 2, 3, 385-393. http://dx.doi.org/10.3390/ijerph2005030002.

[20] Orloff K, Mistry K & Metcalf S (2009) Biomonitoring for environmental exposures to arsenic. Journal of Toxicology and Environmental Health, B Critical Review 12, 509–524. http://dx.doi.org/10.1080/10937400903358934.

[21] Oskarson AL, Jorham L, Sindberg J, Nilson N & Abanus L (1992) Lead poisoning in cattle- Transfer of Lead to milk. Science of the Total Environment 111, 83-94. http://dx.doi.org/10.1016/0048-9697(92)90348-V.

[22] Pathak N & Khandelwal S (2006) Oxidative stress and apoptotic changes in murine splenocytes exposed to cadmium. Toxicology 220, 26–36. http://dx.doi.org/10.1016/j.tox.2005.11.027.

[23] Pathak N & Khandelwal S (2007) Cytoprotective and immunomodulating properties of piperine on murine splenocytes: An in vitro study. European Journal of Pharmacology 576, 160–170. http://dx.doi.org/10.1016/j.ejphar.2007.07.033.

[24] Prabu SM, Muthumani M & Shagirtha K (2013) Quercetin potentially attenuates cadmium induced oxidative stress mediated cardiotoxicity and dyslipidemia in rats. European Review on Medical Pharmacological Science. 17, 282 – 295.

[25] Roy AV (1970) Determination of ALP in human serum. Clinical Chemistry 16, 431-431.

[26] Sami K & Druzykli AL (2003) Predicated spatial distribution of naturally occurring arsenic, selenium and uranium in groundwater in groundwater in South Africa Reconnaisance survey (Report No 1236/1/03). Pretoria: Water Research Commission.

[27] Scheifler R, Coeurdassier M, Morilhat C, Bernard N, Faivre B, Flicoteaux P, Giraudoux P, Noël M, Piotte P & Rieffel D (2006) Lead concentrations in feathers and blood of common blackbirds (Turdus merula) and in earthworms inhabiting unpolluted and moderately polluted urban areas. Science of the Total Environment 371, 197 - 205. http://dx.doi.org/10.1016/j.scitotenv.2006.09.011.

[28] Shi H, Shi X & Liu KJ (2004) Oxidative mechanism of arsenic toxicity and carcinogenesis. Molecular Cell Biochemistry 255, 67–78. http://dx.doi.org/10.1023/B:MCBI.0000007262.26044.e8.

[29] Shore RF (1995) Predicting cadmium, lead and fluoride levels in small mammals from soil residues and by species-species extrapolation. Journal of Environmental Pollution 88, 3, 333-340. http://dx.doi.org/10.1016/0269-7491(95)93447-8.

[30] Talmage SS (1991) Walton BT. Small mammals monitors of environmental contaminants. Environmental Contamination and Toxicology 119, 47-145.

[31] Timbrell JA (1991) Principle of biochemical toxicology. 2nd edn. (Taylor and Francis ed.) USA p.369.

[32] Toens PD, Stadler W and Wullschleger NJ (1998) the association of groundwater chemistry and geology with a typical lymphocytes (as a biological indicator) in the Pofadder area, (Report No. K5/839). North Western Cape: WRC.

[33] Turkdogan MK, Kulicel F, Kara K, Tuncer I & Uyang I (2003) Heavy metals in soils, vegetable and fruit in the endermic upper gastro intestinal cancer region of Turkey. Environmental Toxicology and Pharmacology 13, 3, 175-179. http://dx.doi.org/10.1016/S1382-6689(02)00156-4.

[34] Valko M, Morris H & Cronin MT (2005) Metals, toxicity and oxidative stress. Current Medicinal Chemistry 12, 1161–1208. http://dx.doi.org/10.2174/0929867053764635.

[35] Wang W & Fisher NS (1999) Delineating metal accumulation pathways for marine invertebrates. Science of the Total Environment 238, 459-472.

[36] Wright PJ & Plummer DT (1974) the use of urinary enzyme measurement to detect renal damage caused by nephritic compounds. Biochemical Pharmacology 23, 65-73. http://dx.doi.org/10.1016/S0048-9697(99)00158-8.

[37] Zhang ZF, Shao MY & Kang KH (2005) Changes of enzyme activity and hematopoiesis in Chinese prawn Fenneropenaeus chinensis (Osbeck) induced by white spot syndrome virus and zymosan A. Aquaculture Reseach 36, 674-81. http://dx.doi.org/10.1111/j.1365-2109.2005.01272.x.

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