Effect of three anticoccidials on pharmacokinetics of tilmicosin in broiler chickens

  • Authors

    • Mohamed El-Hewaity
  • Anticoccidials, Broiler Chickens, Kinetic, Tilmicosin.
  • The disposition kinetic of tilmicosin (25mg/kg) was studied following oral administration alone, pretreated with amprolium (240 ppm), pretreated with diclazuril (2.5 ppm) and pretreated with toltrazuril (25 ppm) in broiler chickens. The serum tilmicosin concentrations were determined by microbiological assay technique using Bacillus subtilis (ATCC 6633) as the test organism. Following oral administration of tilmicosin, the disposition curve was best described by two-compartment open model. The maximum serum concentration (Cmax) was 1.90 ± 0.11, 1.27 ± 0.13, 1.50 ± 0.14 and 1.41 ± 0.11µg/ml for tilmicosin alone and in the presence of amprolium, diclazuril and toltrazuril, respectively. The elimination half-life (T0.5 (el)) was significantly decreased (5.28 ± 0.30, 5.88 ± 0.33, 6.03 ± 0.25 h, respectively) in amprolium, diclazuril and toltrazuril pretreated broiler chicken compared to tilmicosin alone (7.30 ± 0.41 h). The outcomes illustrated a significant decrease in the interval between doses in amprolium, diclazuril and toltrazuril pretreated broiler chicken compared to tilmicosin alone. Amprolium diclazuril and toltrazuril, resulted in a significance decrease in AUC (12.02 ± 1.14, 15.50 ± 1.26 and 14.56 ± 1.46 µg.h.ml-1, respectively) compared to tilmicosin alone (21.98±1.83 µg.h.ml-1). It is concluded that the administration of amprolium, diclazuril and toltrazuril before tilmicosin would altered its pharmacokinetic profile in broiler chicken.

  • References

    1. [1] Abou El-Sooud, K. 2003, Influence of albendazole on the disposition kinetics and milk antimicrobial equivalent activity of enrofloxacin in lactating goats. Pharmacology Research, 48, 389-395. http://dx.doi.org/10.1016/S1043-6618(03)00179-8.

      [2] Abu-Basha, E.A., Al-Shunnaq, A.F. and Gehring, R. 2012, Comparative pharmacokinetics and bioavailability of two tylosin formulations in chickens after oral administration. J HELLENIC VET MED SOC, 63, 159-166.

      [3] Abu-Basha, E. A., Idkaidek, N. M. and Al-Shunnaq, A. F. 2007, Pharmacokinetics of tilmicosin (Provitil powder and pulmotil liquid AC) oral formulations in chickens. Veterinary Research Communications, 31, 477–485. http://dx.doi.org/10.1007/s11259-006-3543-6.

      [4] Arret, B., Johnson, D. and Kirshboum, A. 1971, Outline of details for microbiological assay of antibiotics, second revision. Pharmacology Science, 60, 1689-1694. http://dx.doi.org/10.1002/jps.2600601122.

      [5] Atef, M., El-Gendi, A.Y., Aziza, M.M., Abd El-Aty, A.M. 2010, Effect of three anthelmentics on disposition kinetic of florfenicol in goats. Food and Chemical Toxicology, 48, 3340-3344. http://dx.doi.org/10.1016/j.fct.2010.08.039.

      [6] Atef, M., El-Gendi, A.Y., Aziza, M.M., Kamel, G. 2009, Pharmacokinetic Assessment of Tylosin Concomitantly Administered with Two Anticoccidials Diclazuril and Halofuginone in Broiler Chickens. Advances in Environmental Biology, 3, 210-218.

      [7] Botsolou, N. A. and Fletouris, D. J. 2001, “Drug residues in foods,†in Antimicrobial Growth Promoters, pp. 189–190, Marcel Dekker, New York, NY, USA.

      [8] Brander, G.C., Pugh, D.M., Baywater, R.J. and Jenkins, W.L. 1991, Veterinary Applied Pharmacology and Therapeutics. Fifth Ed. The English language Book Society and Boilliere, Trindall, London.

      [9] Craig, A.W. and Suh, B. 1980, Protein binding and the antibacterial effects. Method for the determination of protein binding, in: LORIAN, V. (Ed.) Antibiotics in Laboratory Medicine, 3rd edn, (Baltimore, Maryland, USA, Williams & Wilkins) pp. 367-402.

      [10] Dimitrova, D., Кatsarov, V., Dimitrov, D. and Tsoneva, D. 2011, Pharmacokinetics of tilmicosin after oral application of Pulmotil G 200 – premix in pigs. AGRICULTURAL SCIENCE AND TECHNOLOGY, 3, 318 – 322.

      [11] El-Banna, H. A., El- Hewaity, M.H. and Amera Abd El Latif 2013, Influence of amprolium and toltrazuril on the disposition kinetics of levofloxacin in broiler chickens. Egyptian.Acadamic Journal of Biology Science, 5, 1-10.

      [12] El-Sayed, M.G., El-Komy, A., El-barawy, A.M. and Gehan, M.E.A. 2014, Pharmacokinetical Interactions of Amoxicillin and Amprolium in Broiler Chickens. Physiology and Pharmacology Advances, 4, 515-524. http://dx.doi.org/10.5455/jppa.20141208013007.

      [13] El-Sayed, M.G., El-Komy, A., El-barawy, A.M. and Gehan, M.E.A. 2015, PHARMACOKINETICAL INTERACTIONS OF LINCOMYCIN AND AMPROLIUM IN BROILER CHICKENS Journal of Science, 5, 734-743.

      [14] Haddad, N. S., Pedersoli, W. M., Ravis, W. R., Fazel,M. H. and Carson, R. L. 1985, “Pharmacokinetics of gentamicin at steady-state in ponies: serum, urine, and endometrial concentrations,†The American Journal ofVeterinary Research, 46, 1268–1271.

      [15] Jones, F.T. and Ricke, S.C. 2003, Observations on the history of the development of antimicrobials and their use in poultry feeds. Poultry Science, 82, 613–617. http://dx.doi.org/10.1093/ps/82.4.613.

      [16] Kempf, I., Reeve-Johnson, L., Gesbert, F. and Guittet, M. 1997, Efficacy of tilmicosin in the control of experimental mycoplasma gallisepticum infection in chickens, Avian Diseases, 41, 802–807. http://dx.doi.org/10.2307/1592332.

      [17] Main, B.W., Means, J.R., Rinkema, L.E., Smith, W.C. and Sarazan, R.D. 1996, cardiovascular effects of the macrolide antibiotic tilmicosin, administered alone and in combination with propranolol or dobutamine, in conscious unrestrained dogs. Journal of Veterinary Pharmacology and Therapeutics, 19, 225–232. http://dx.doi.org/10.1111/j.1365-2885.1996.tb00042.x.

      [18] McDougald, L.R. and Reid, W.M. 1997, Coccidiosis. In, B.W. Calnek (Eds). Diseases of Poultry. 10th Ed. Iowa State University Press, Ames, 865- 883.

      [19] Papich, M.G. and Riviere, J.E. 2001, Chloramphenicol and derivatives, macrolides, lincosamides and miscellaneous antimicrobials. Veterinary Pharmacology and Therapeutics, 8th edn (Iowa State Press, Ames, IA), 880–881.

      [20] Prescott, J. F. 2000, Macrolides and pleuromyilins, in Antimicrobial Therapy in Veterinary Medicine, pp. 229–262, Iowa State University Press, Ames, Iowa, USA, 3rd edition.

      [21] Scott, P.R., McGowan, M., Sargison, N.D., Penny, C.D. and Lowman, B.G. 1996, Use of Tilmicosin in a Severe Outbreak of Respiratory Disease in Weaned Beef Calves. Australian Veterinary Journal, 73, 62-64. http://dx.doi.org/10.1111/j.1751-0813.1996.tb09967.x.

      [22] Shen, J., Li, C., Jiang, H,, Zhang, S., Guo, P., Ding, S. and Li X. 2005, Pharmacokinetics of tilmicosin after oral administration in swine. American Journal of Veterinary Research, 66, 1071-1074. http://dx.doi.org/10.2460/ajvr.2005.66.1071.

      [23] Snedecor, G.W. and Cochran, T. 1976, “Statistical Methods†6th ed. pp. Ames, lowa U.S.A., 502-503.

      [24] Soliman, A. M. and Sedek, M. 2016, Pharmacokinetics and Tissue Residues of Tylosin in Broiler Chickens. Pharmacology & Pharmacy, 7, 36-42. http://dx.doi.org/10.4236/pp.2016.71006.

      [25] Taylor, M.A., Catchpole, J., Marshall, J., Marshall, R. N. and Hoeben, D. 2003, Histopathological observations on the activity of diclazuril (Vecoxan) against the endogenous stages of Eimeria crandallis in sheep. Veterinary Parasitology, 116, 305–314. http://dx.doi.org/10.1016/S0304-4017(03)00256-5.

      [26] Vertommen, M. H., Peek, H. W. and Laan, A. 1990, Efficacy of toltrazuril in broilers and development of a laboratory model for sensitivity testing of Eimeria field isolates. Veterinary Quarterly, 12, 183-192. http://dx.doi.org/10.1080/01652176.1990.9694264.

      [27] Yamaoka, K., Nakagawa, T. and Uno, T. 1978, Statistical moment in pharmacokinetics. Journal of Pharmacokinetic and Biopharmaceutics, 6, 547-558. http://dx.doi.org/10.1007/BF01062109.

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    El-Hewaity, M. (2016). Effect of three anticoccidials on pharmacokinetics of tilmicosin in broiler chickens. International Journal of Pharmacology and Toxicology, 4(2), 150-153. https://doi.org/10.14419/ijpt.v4i2.6519