Paracetamol induced hepatic toxicity and amelioration by cinnamon in rats

  • Authors

    • Ashraf Elkomy professor of pharmacology
    • Mohamed Aboubakr
    • Ahmed Soliman
    • Ahmed Abdeen
    • Afaf Abdelkader
    • Haitham Hekal
    2016-09-26
    https://doi.org/10.14419/ijpt.v4i2.6529
  • Cinnamon, Hepato-Protective, Paracetamol, Rats.
  • The study was designed to evaluate the hepato-protective activity of aqueous extract of cinnamon in acute experimental liver injury induced by paracetamol. Twenty four male albino rats were randomly divided into four groups (six rats in each). Group I rats received distilled water for 15 days and served as a vehicle control. The animals in the group II were given single oral administration of paracetamol (1 g/kg), 1 h after last distilled water administration and acts as paracetamol toxic control group. Groups III and IV received aqueous extract of cinnamon (200 and 400 mg/kg bwt), respectively, once daily for 15 consecutive days followed by a single oral administration of paracetamol (1 g/kg), 1 h after the last cinnamon dose administration. The degree of hepatoprotection was measured using liver enzymes (Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), bilirubin, albumin, and lipid profile levels. Also, histopathological examinations of liver were done. The significantly disturbed liver functions by paracetamol toxicity were restored to nearly normal values by administration of cinnamon. Also, the change in biochemical parameters in groups received paracetamol alone was modified towards the normal values in groups given paracetamol and cinnamon. Histopathological effect of paracetamol on liver was also markedly decreased by co-administration of cinnamon. Our findings concluded that cinnamon aqueous extracts possessed hepato-protective activity against paracetamol induced hepatic toxicity in rats.

  • References

    1. [1] Abraham P. (2005) Oxidative stress in paracetamol-induced pathogenesis: (I) Renal damage. Indian J Biochem Biophys. ; 42:59–62.

      [2] Ahmed M, Khater MR. (2001) Evaluation of the protective effect of Ambrosia maritime extract on acetaminophen-induced liver damage. J Ethnopharmacol. ; 75:169–174. http://dx.doi.org/10.1016/S0378-8741(00)00400-1.

      [3] Bakkali F, Averbeck S, Averbeck D. (2008) Biological effects of essential oils - a review. Food Chem Toxicol; 46: 446-475. http://dx.doi.org/10.1016/j.fct.2007.09.106.

      [4] Bonkovsky, H.L., Kane, R.E., Jones, D.P., Galinsky, R.E., Banner, R. (1994). Acute hepatic and renal toxicity from low doses of acetaminophen in the absence of alcohol abuse or malnutrition: evidence for increased susceptibility to drug toxicity due to cardiopulmonary and renal insufficiency. Hepatology 19(5): 1141-1448. http://dx.doi.org/10.1002/hep.1840190511.

      [5] Carmo ES, Lima ED, de Souza EL, de Sousa FB. (2008) Effect of Cinnamomum zeylanicum blume essential oil on the growth and morphogenesis of some potentially pathogenic Aspergillus species. Braz J Microbiol; 39:91-97. http://dx.doi.org/10.1590/S1517-83822008000100021.

      [6] Diadelis, R., Jan, N.M., Commandeur, E.D., Nic, P.E.,Vermeulen. (1995). Mechanism of protection of Lobenzarit against paracetamol-induced toxicity in rat hepatocytes Environmental Toxicology and Pharmacology Section 293: 300.

      [7] Gad FA, Abd Allah OA, Ayman Farid AS, Fararh KM (2013) Biochemical, Histopathological and immunohistochemical changes in paracetamol, fish oil and thioacetamide treated rats. Benha Vet J. 25(1) 18-28.

      [8] Gruenwald J, Freder J, Armbruester N. (2010) Cinnamon and health. Crit Rev Food Sci Nutr; 50:822-834. http://dx.doi.org/10.1080/10408390902773052.

      [9] Harries ML. (1989) Carleton´s Histopathological Technique. 5thed. Oxford Univ. Press, New York, Toronto. P. 33-48.

      [10] Iweala, E.E.J.,Osundiya, A.O. (2010). Biochemical, Haemayological and Histological Effectsnof Dietary Supplementation with leaves of GnetumafricanumWelw on Paracetamol induced Hepatotoxicity in rats. International Journal of Pharmacology 6(6): 872-879. http://dx.doi.org/10.3923/ijp.2010.872.879.

      [11] Jayaprakasha GK, Negi PS, Jena BS, Jagan Mohan Rao L. (2007) Antioxidant and antimutajenic activities of Cinnamomum zeylanicum fruit extracts. J Food Compos Anal; 20:330-336. http://dx.doi.org/10.1016/j.jfca.2006.07.006.

      [12] Khan A., Safdar, M. Khan, M.M.A. Khattak, K.N. and Anderson, R.A. (2003). “Cinnamon improves glucose and lipids of people with type 2 diabetes,†Diabetes Care, 26 (12) 3215–3218. http://dx.doi.org/10.2337/diacare.26.12.3215.

      [13] Kim S.H., Hyun, S.H. and Choung, S.Y. (2006). Anti-diabetic effect of cinnamon extract on blood glucose in db/db mice. Journal of Ethnopharmacology, 104, (1-2) 119–123. http://dx.doi.org/10.1016/j.jep.2005.08.059.

      [14] Mayuren C., Reddy V.V., Priya S.V., and Devi V.A., (2010) Protective effect of Livactine against CCL4 and paracetamol induced hepatotoxicity in adult Wister rats. North American Journal of Medical Sciences, (2) 492-495. http://dx.doi.org/10.4067/S0716-97602009000100009.

      [15] Moselhy, S.S. and Ali, H.H. (2009). Hepatoprotective effect of Cinnamon extracts against carbon tetrachloride induced oxidative stress and liver injury in rats. Biol Res 42: 93-98.

      [16] Muthuraman A., Sood S., and Singla S.K., (2011) the anti-inflammatory potential of phenolic compounds from Emblica officinalis L. in rat. Inflammopharmacology, 19: 327-334. http://dx.doi.org/10.1007/s10787-010-0041-9.

      [17] Nishida S, Kikuichi S, Yoshioka S, Tsubaki M, Fujii Y, Matsuda H. (2003) Induction of apoptosis in HL-60 cells treated with medicinal herbs. Am J Chin Med; 31:551- 562. http://dx.doi.org/10.1142/S0192415X03001211.

      [18] Preuss HG, Echard B, Polansky MM, Anderson R. (2006) Whole cinnamon and aqueous extracts ameliorate sucrose-induced blood pressure elevations in spontaneously hypertensive rats. J Am Coll Nutr; 25: 144- 150. http://dx.doi.org/10.1080/07315724.2006.10719525.

      [19] Rajkapoor, B.; Venugopal, Y.; Anbu, J.; Harikrisshnan, N.; Gobinath, M. and Ravichandran, V. (2008). Protective effect of Phyllanthuspolyphylluson acetaminophen induced hepatotoxicity in rats. Pakistan. J. Pharm. Sci., 21(1): 57 -62.

      [20] Setty, S.R., Quereshi, A.A., Swamy, A.H., TusharPatil, T., Prakash, T., Prabhu, K., Gouda, A.V. (2007). Hepatoprotective activity of Calotropisprocera flowers against paracetamol-induced hepatic injury in rats. Fitoterapia (78):451–454. http://dx.doi.org/10.1016/j.fitote.2006.11.022.

      [21] Shalaby MA and Saifan HY (2014) some pharmacological effects of cinnamon and ginger herbs in obese diabetic rats J Intercult Ethnopharmacol. 3(4): 144–149.

      [22] Shalaby MA, Hamowieh AR. (2010) Safety and efficacy of Zingiber officinale roots on fertility of male diabetic rats. Food Chem Toxicol. ; 48:2920–2924. http://dx.doi.org/10.1016/j.fct.2010.07.028.

      [23] Shihabudeen, M.; Priscilla H.; Thirumurugan K. (2011). Cinnamon extract inhibits glucosidase activity and dampens postprandial glucose excursion in diabetic rats. Nutr Metab; 8:1-11.

      [24] Sreedhar, V., Nath, L.K., Nath, M.S., RajaSekhar, K.K., Shankarananth, V. (2011). Hepatoprotective Activity of Vitextrifoliata roots against Paracetamol- Induced Hepatic Injury in rats. Journal of Pharmacy Research 4(2): 551-553.

      [25] Subash Babu P, Prabuseenivasan S, Ignacimuthu S. (2007). Cinnamaldehyde-a potential antidiabetic agent. Phytomedicine; 14: 15-22. http://dx.doi.org/10.1016/j.phymed.2006.11.005.

      [26] Tung YT, Chua MT, Wang SY, Chang ST. (2008) Antiinflammation activities of essential oil and its constituents from indigenous cinnamon (Cinnamomum osmophloeum) twigs. Bioresour Technol; 99: 3908-3913. http://dx.doi.org/10.1016/j.biortech.2007.07.050.

      [27] Yang YC, Lee HS, Lee SH, Clark JM, Ahn YJ. (2005) Ovicidal and adulticidal activities of Cinnamomum zeylanicum bark essential oil compounds and related compounds against Pediculus humanus capitis (Anoplura: Pediculicidae). Int J Parasitol; 35:1595-1600. http://dx.doi.org/10.1016/j.ijpara.2005.08.005.

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    Elkomy, A., Aboubakr, M., Soliman, A., Abdeen, A., Abdelkader, A., & Hekal, H. (2016). Paracetamol induced hepatic toxicity and amelioration by cinnamon in rats. International Journal of Pharmacology and Toxicology, 4(2), 187-190. https://doi.org/10.14419/ijpt.v4i2.6529