Free radical scavenging activity of aqueous and alcoholic extracts of Glycyrrhiza glabra Linn. measured by ferric reducing antioxidant power (FRAP), ABTS bleaching assay (αTEAC), DPPH assay and peroxyl radical antioxidant assay

  • Authors

    • Mradu Gupta Institute of Post Graduate Ayurvedic Education and Research, 294 / 3/ 1, A. P. C. Road, Kolkata, India – 700009
    • Nandita Karmakar Institute of Post Graduate Ayurvedic Education and Research, 294 / 3/ 1, A. P. C. Road, Kolkata, India – 700009
    • Saswati Sasmal Institute of Post Graduate Ayurvedic Education and Research, 294 / 3/ 1, A. P. C. Road, Kolkata, India – 700009
    • Saikat Chowdhury Institute of Post Graduate Ayurvedic Education and Research, 294 / 3/ 1, A. P. C. Road, Kolkata, India – 700009
    • Suman Biswas Institute of Post Graduate Ayurvedic Education and Research, 294 / 3/ 1, A. P. C. Road, Kolkata, India – 700009
    2016-11-27
    https://doi.org/10.14419/ijpt.v4i2.6578
  • ABTS, Antioxidant Capacity, DPPH, FRAP, Glycyrrhiza Glabra.

  • Abstract

    The present study evaluates the in vitro antioxidant free radical scavenging capacities of aqueous and alcoholic extracts of Glycyrrhiza glabra since this medicinal plant has been traditionally used in Ayurvedic system of medicine as a tonic, rejuvenator, demulcent and expectorant. Total phenolic content of alcoholic extract and aqueous extract was found as 18.70 and 23.26 Gallic acid equivalents/mg while the total flavonoid content was assessed as 15.03 and 6.62 Quercetin equivalents/mg respectively. IC50 (µg/ml) of alcoholic, aqueous extract and Ascorbic acid was found to be 65.064±3.538, 73.423±9.057 and 18.156±1.356 in Hydrogen Peroxide Radical Scavenging, 1.424±0.013, 1.632±0.052 and 0.879±0.273 in DPPH radical scavenging, and 335.948±29.040, 251.891±6.330 and 6.142±0.061 during FRAP essay. Similarly, during ABTS radical scavenging, IC50 (µg/ml) was assessed as 575±26.694, 683.9±49.220 and 6.337±0.167 for alcoholic, aqueous extract and Trolox respectively. While both the extracts showed substantial and significant free radical scavenging activity during the study which is compared to that of standard drug, the alcoholic extract of Glycyrrhiza glabra exhibits higher in vitro antioxidant activity than the aqueous extract in all assessment methods except FRAP, possibly due to higher concentrations of flavonoids in it, validating and confirming the therapeutic properties ascribed to it in Ayurvedic texts.

  • References

    1. [1] Agati G, Azzarello E, Pollastri S& Tattini M 2012, Flavonoids as antioxidants in plants: location and functional significance. Plant Sci 196, 67–76. http://dx.doi.org/10.1016/j.plantsci.2012.07.014.

      [2] Anil KV, Babul D& Rama T 2011, Evaluation of Anti-ulcerogenic properties from the root of Flemingia strobilifera. Journal of Basic and Clinical Pharmacy 2 (1), 33-39.

      [3] Aparadh VT, Naik VV&Karadge BA 2012, Antioxidative Properties (TPC, DPPH, FRAP, Metal Chelating Ability, Reducing Power and TAC) within some Cleome Species. Ann. Bot. (Roma) 2, 49-56.

      [4] Baba SA &Malik SA 2015, Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii. Journal of Taibah University for Science 9, 449-454. http://dx.doi.org/10.1016/j.jtusci.2014.11.001.

      [5] Basniwal PK, Suthar M, Rathore GS, Gupta R, Kumar V, Pareek A & Jain D 2009, In-vitro antioxidant activity of hot aqueous extract of Helicteres isora Linn. fruits. Natural Product Radiance 8 (5), 483-87.

      [6] Bravo L 1998, Polyphenols: chemistry, dietary sources, metabolism and nutritional significance. Nutr. Rev 56, 317–333. http://dx.doi.org/10.1111/j.1753-4887.1998.tb01670.x.

      [7] Chang C, Yang M, Wen H& Chern J 2002, Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal 10, 178–182.

      [8] Everest A & Ozturk E 2005, Focusing on the ethnobotanical uses of plants in Mersin and Adana provinces (Turkey). Journal of Ethnobiology and Ethnomedicine1 (6). http://dx.doi.org/10.1186/1746-4269-1-6.

      [9] Geetha S, Sai-Ram M, Mongia SS, Singh V & Ilavazhagan G 2003, Evaluation of antioxidant activity of leaf extract of sea buckthorn (Hippophae rhamnoides L.) on chromium (VI) induced oxidative stress in albino rats. J. Ethnopharmacol 87, 247–251. http://dx.doi.org/10.1016/S0378-8741(03)00154-5.

      [10] Harborne JB 1984, Phytochemical Methods: A guide to Modern Technique of Plant Analysis, 2nd edition, Chapmann and Hall: London. http://dx.doi.org/10.1007/978-94-009-5570-7.

      [11] Hemalatha S, Lalitha P& Arulpriya P 2010, Anti-oxidant activities of the extracts of the aerial roots of Pothos aurea (Linden ex Andre). Der. Pharma. Chemica 2 (6), 84-89.

      [12] Keser S, Celik S, Turkoglu S, Yilmaz O & Turkoglu I 2012, Hydrogen Peroxide Radical Scavenging and Total Antioxidant Activity of Hawthorn. Chemistry Journal 02 (1), 9-12.

      [13] Kirtikar KR & Basu BD 1989, Indian Medicinal Plants, vol. 2, Allahabad, pp. 312-313.

      [14] Nimse SB& Pal DK 2015, Free radicals, natural antioxidants, and their reaction mechanisms, (Review Article) RSC Adv5, 27986-28006. http://dx.doi.org/10.1039/C4RA13315C.

      [15] Nishaa S, Vishnupriya M, Sasikumar JM, Hephzibah PC & Gopalakrishnan VK 2012, Antioxidant activity of ethanolic extract of Maranta arundinacea L. tuberous rhizomes. Asian Journal of Pharmaceutical and Clinical Research 5 (4), 85-88.

      [16] Oyaizu M 1986, Studies on product of browning reaction prepared from glucose amine. Jpn. J. Nutr 44, 307-315. http://dx.doi.org/10.5264/eiyogakuzashi.44.307.

      [17] Özcelik B, Lee JH& Min DB 2003, Effects of light, oxygen and pH on the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method to evaluate antioxidants. J. Food Sci 68, 487-490. http://dx.doi.org/10.1111/j.1365-2621.2003.tb05699.x.

      [18] Re R, Pellegrini N, Proteggente A, Pannala A, Yang M& Rice-Evans C 1999, Antioxidant activity applying an improved ABTS radical cation decolourization assay. Free Radic. Biol. Med 26, 1231-1237. http://dx.doi.org/10.1016/S0891-5849(98)00315-3.

      [19] Sharma PC, Yelne MB& Dennis TJ 2001, Database on medicinal plants used in Ayurveda, vol. 8, Central Council for Research in Ayurveda & Siddha, Department of Indian system of medicine, Govt. of India, New Delhi, pp. 42 -58.

      [20] Sharma PV 1995, Introduction to Dravyaguna (Indian Pharmacology), vol. 2, Chaukhamba Orientalia: Varanasi, India, pp. 734-736.

      [21] Shimoi K, Masuda S, Shen B, Furugori M& Kinze N 1996, Radioprotective effects of antioxidative plant flavonoids in mice. Mutat. Res. Fund. Mol 350, 153–161. http://dx.doi.org/10.1016/0027-5107(95)00116-6.

      [22] Shukla S, Mehta A, Bajpai KV& Shukla S 2009, In vitro antioxidant activity and total phenolic content of ethanolic leaf extract of Stevia rebaudiana Bert. Food Chem. Toxicol 47, 2338–2343. http://dx.doi.org/10.1016/j.fct.2009.06.024.

      [23] Tupe RS, Kemse NG& Khaire AA 2013, Evaluation of Antioxidant potentials and Total Phenolic contents of Selected Indian Herbs powder extracts. Indian Food Research journal 20 (3), 1053-63.

      [24] Usha Sri B, Ibrahim M& Mahendra Kumar CB 2012, Antioxidant activity and total flavonoids content of different parts of Azadirachta indica A. Juss. Journal of Medicinal Plants Research 6 (46), 5737-5742.

      [25] Wang SY& Jiao H2000, Scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydroxyl radicals, and singlet oxygen. J. Agric. Food. Chem 48 (11), 5677-84. http://dx.doi.org/10.1021/jf000766i.

      [26] Živković J, Zeković Z, Mujić I, Vidović S, Cvetković D, Lepojević Ž, Nikolić G&Trutić N 2010, Scavenging Capacity of Superoxide Radical and Screening of Antimicrobial Activity of Castanea sativa Mill. Extracts. Czech. J. Food. Sci 28 (1), 61–68.

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  • How to Cite

    Gupta, M., Karmakar, N., Sasmal, S., Chowdhury, S., & Biswas, S. (2016). Free radical scavenging activity of aqueous and alcoholic extracts of Glycyrrhiza glabra Linn. measured by ferric reducing antioxidant power (FRAP), ABTS bleaching assay (αTEAC), DPPH assay and peroxyl radical antioxidant assay. International Journal of Pharmacology and Toxicology, 4(2), 235-240. https://doi.org/10.14419/ijpt.v4i2.6578

    Received date: 2016-08-08

    Accepted date: 2016-09-10

    Published date: 2016-11-27