Phyto chemical and memory enhancer activity of the 2 polyherbal formulation: comparative activity on AlCl3 induced albino wistar rats
Keywords:EPM, Transfer Latency, Polyherbal Formulations, Cognitive Impairment.
Background: Epidemiological studies of Indian population reveal that dementia is largely a hidden problem in India. Ayurveda claims several plants are beneficial in cognitive disorders. Pharmaco-epidemiological studies reveal that herbal and allopathic learning and memory enhancing medicines are becoming very popular among Indian population.
Methodology: The present study was aimed at investing the effects of Dr. Brain Syrup and Capsule, Ayurvedic polyherbal formulation on memory enhancing activity in albino wistar rats. Drugs were administered through intraperitoneal at therapeutic dose for 28 days to different groups of the rats. Elevated Plus maze (EPM) and Radial Arm maze apparatus were served as the evaluating tool to identify the Transfer Latency (TL) in EPM and Average time taken to reach right arm in Radial Arm Maze models. Cognitive impairment was induced by administering the AlCl3 at dose of 4.2mg/kg i.p.
Result: At the end of 4 weeks, Transfer Latency in model control group increased to 90±0.00 sec as compared to47.66±7.39 sec (p<0.01) in Normal control rats. Dr. Brain Syrup and Capsule were significantly attenuated TL to 19.5±4.27 and 21.16±2.83 sec respectively as compared to Model Control Group after 4 weeks. Moreover, Treatment with Dr. Brain Syrup and Capsule reduced the mean time to find the right arm to 20.83±2.08 and 27.16±1.17 sec (p<0.01) as compared to 129.66±4.60 sec in Model control group after 4 weeks.
Conclusion: Dr. Brain Syrup and Capsule showed significant activity in improvement in Memory by evaluating the TL and Average time period in 2 different instruments in 4 weeks of the drug treatment.
 Parle M, Vasudevan M (2007) Memory Enhancing Activity of Abana: An Indian Ayurvedic Poly-Herbal Formulation. Journal of Health Science 53(1), 43-52. http://dx.doi.org/10.1248/jhs.53.43.
 Shaji KS, Jotheeswaran AT, Girish N, Srikala B, Dias A, Pattabiraman M, Varghese M (2010) The Dementia India Report: prevalence, impact, costs and services for Dementia: Executive Summary, Alzheimerâ€™s and Related Disorders Society of India.
 Shaik A, Syed U (2015) Protective Effect of Centella asiatica against Aluminium-Induced Neurotoxicity in Cerebral Cortex, Striatum, Hypothalamus and Hippocampus of Rat Brain-Histopathological, and Biochemical Approach. J Mol Biomark Diagn 6 (1), 212.
 Yuan CY, Lee YJ, Hsu GS (2012) Aluminum overload increases oxidative stress in four functional brain areas of neonatal rats. J Biomed Sci 19, 51. http://dx.doi.org/10.1186/1423-0127-19-51.
 Nayak P, Sharma SB, Chowdary NV (2010) Augmentation of aluminium induced oxidative stress in rat cerebrum by presence of pro-oxidant (graded doses of ethanol) exposure. Neurochem Res 35, 1681-1690. http://dx.doi.org/10.1007/s11064-010-0230-3.
 Charles PD, Ambigapathy G (2011) Bacopa monniera leaf extract up-regulates tryptophan hydroxylase (TPH2) and serotonin transporter (SERT) expression: implications in memory formation. J Ethnopharmacol 134(1), 5-61. http://dx.doi.org/10.1016/j.jep.2010.11.045.
 Pandey S, Singh B, Mahdi AA (2013) Therapeutic potential of noo-tropic Bacopa monnieri in prevention & treatment of diseases: An Overview. International Journal of Scientific and Innovative Research 1(2), 12-24.
 Jyoti A, Sharma D (2006) Neuroprotective role of Bacopa monniera extract against aluminium-induced oxidative stress in the hippocampus of rat brain. NeuroToxicology 27(4), 451-457. http://dx.doi.org/10.1016/j.neuro.2005.12.007.
 Sharma K, Bhatnagar M, Kulkarni KS (2010) Effect of Convolvulus pluricaulis choisy. and Asparagus racemosus wild on learning and memory in young and old mice: A comparative evaluation. Indian Journal of Experimental Biology 48, 479-485.
 Nalini K, Aroor AR, Karanth KS, Rao A (1992) Effect of Centella asiatica fresh leaf aqueous extract on learning and memory and biogenic amine turnover in albino rats. Fitoterapia 63, 232-237.
 Singh S, Gautam A, Sharma A, Batra A (2010) Centella asiatica (l.): A plant with immense medicinal potential but threatened. International journal of Pharmaceutical Sciences Review and Research 4(2), 9-17.
 Upadhyay SK, Saha A, Bhatia BD, Kulkarni KS (2002) Evaluation of the efficacy of mentat in children with learning disability Placebo- Controlled Double-Blind clinical trial. Neurosciences Today 3(6), 184-188.
 Bhanumathy M, Chandrasekhar SB, Chandur U, Somasundaram T (2010) Phyto-Pharmacology of Celastrus paniculatus: An Overview. International journal of Pharmaceutical Sciences and Drug Research 2(3), 176-181.
 Murthy KS, Ramlu DR (2011) Biological activity and phytochemical screening of the oleoresin of Shorea robusta. Tropical and subtropical Agroecosystems, 787-791.
 Kulkarni SK (2012) Handbook of Experimental Pharmacology. Vallbh Prakashan, Mumbai, 4th Edition, 146-148.
 Ladde S, Gouda ST, Rao VN, Verma R (2011) Evaluation of Memory enhancing activity of SR-105 in Experimental Animals. IJRAP 2(3), 973-977.
 Wood AJJ (2004) Drug Therapy: Alzheimerâ€™s disease. N.Engl.J.Med 351, 56-67. http://dx.doi.org/10.1056/NEJMra040223.