Evaluation of the protective effect of Cinnamomum Zeylanicum on cadmium testicular toxicity and Nrf2 gene expression in albino rats

  • Authors

    • Samer Ibrahim lecturer of toxicology
    • Seham Y.Abo-Kora lecturer of pharmacology,faculty of veterinary medicine. Benha university
  • Cadmium Toxicity, Cinnamon Extract, Nrf2, Antioxidants.
  • Cadmium is an industrial pollutant that may exert specific toxic effect on mammals. The aim of study was to investigate the protective effect of Cinnamon on male reproductive system of albino rat induced by cadmium toxicity. Forty male albino rats were divided into 4 groups. 1st  Group administrated saline and kept as control.2nd Group administrated 200mg/kg / day Cinnamon extract. 3rd Group administrated 20mg/kg/day cadmium chloride.4th Group administrated 200mg/kg/day Cinnamon extract and 20mg/kg/day cadmium chloride orally for 8 weeks. . Our results revealed significant decrease in testosterone hormone level, sperm viability, and significant increase in sperm abnormality. An increase in expression of Nrf2 gene was recorded. Pathological changes in testes showed focal degeneration with loss of spermatogenic series in the seminiferous tubules. All of the above mentioned results were significantly improved in 4th group. In conclusion, Cinnamon extract has a protective effect on the testicular damage induced by cadmium chloride.

  • References

    1. [1] Acharya UR, Mishra M, Patro J &Panda MK (2008) Effect of vitamin C and E onspermatogenesis in mice exposed to cadmium. . Reproductive Toxicology; 25(1):84-88 https://doi.org/10.1016/j.reprotox.2007.10.004.

      [2] Bailey RA (2008) Design of comparative experiments. Cambridge University Press. Firstedition: 16-128 https://doi.org/10.1017/cbo9780511611483.

      [3] Balen HA &Rutherford JA (2007) Management of infertility. BMJ 335 7620:608-711 https://doi.org/10.1136/bmj.39324.662049.80.

      [4] Bancroft JD, Gamble A (2008) Theory and practice of histological techniques. 6th ed. Churchill Livingstone, New York, London, :165-175

      [5] Bernhoft RA (2003) Cadmium toxicity and treatment. The Scientific World Journal 2013(Article ID 394652):7

      [6] Blanco A, Moyano MR & Molina AM (2009) Quantitative study of Leydig cell populations in mice exposed to low doses of cadmium. Bull. Environ. Contam. Toxicol., 82: 756–760Bull. Environ. Contam. Toxicol82:725-760 https://doi.org/10.1007/s00128-009-9700-1.

      [7] Bu T, Mi .Y, Zeng. W& ZC (2011) Protective effect of quercetin on cadmium-induced oxidative toxicity on germ cells in male mice. Anat Rec (Hoboken) 294:520-526 https://doi.org/10.1002/ar.21317.

      [8] ChenJ &Shaikh Z. A. (2009) Activation of Nrf2 by cadmium and its role in protection against cadmium-induced apoptosis in rat kidney cells. Toxicology and Applied Pharmacology, 241(1):81-89 https://doi.org/10.1016/j.taap.2009.07.038.

      [9] Darbre PD (2006) Metalloestrogens: an emerging class of inorganic xenoestrogens with potential to add to the oestrogenic burden of the human breast. . J. Appl. Toxicol., 26:191-197 https://doi.org/10.1002/jat.1135.

      [10] Doaa M, Saber A, Omar A &Soliman. (2014) Effect of Cadmium on the Testes of Adult Albino Rats And The Ameliorating Effect of Zinc and Vitamin E. British Journal of Science11(1):72-95

      [11] Ekins R (1998) the science of free Testosterone Measurment. Proc. UK NEQAS Meeting 3:35-39

      [12] El-Demerdash. F M, Yousef .M I, Kedwany .F S& Baghdadi HH (2004) Cadmium-induced changes in lipid peroxidation, blood hematology, biochemical parameters and semen quality of male rats: protective role of vitamin E and beta carotene. . Food ChemToxicol 42:1563-1571 https://doi.org/10.1016/j.fct.2004.05.001.

      [13] El-Nekeety. A.A., El-Kady A.A., M.S. S, N.S. H& M.A. A-W (2009) Protective effect of Aquilegia vulgaris (L.) against lead acetate-induced oxidative stress in rats,. Food Chem. Toxicol. 47:2209-2215 https://doi.org/10.1016/j.fct.2009.06.019.

      [14] El-Shahat AE, Gabr A, MekiAR&Mehana ES (2009) Altered testicular morphology and oxidative stress induced by cadmium in experimental rats and protective effect of simultaneous green tea extract. Int. J. Morphol.,27(3):757-764 https://doi.org/10.4067/s0717-95022009000300020.

      [15] Henson MC&Chedrese PJ (2004) Endocrine disruption by cadmium, a common environmental toxicant with paradoxical effects on reproduction. Exp. Biol. Med., 229:383-392

      [16] JahromiVH, ParivarK&Forozanfar M (2011) The Effect of cinnamon extracton spermatogenesis hormonal axis of pituitary gonad in mice. Iran.J. Appl. Anim. Sci 1:99-103

      [17] Jedlinska MG, K. Bomba T, Jakubowski BJ, Rotkiewicz&Penkowski. A (2006) Impact of oxidative stress and supplementation with vitamins E and C on testes morphology in rats. J. Reproduc 52:203-209 https://doi.org/10.1262/jrd.17028.

      [18] Johnson MD, Kenney N, Stoica A, Hilakivi-Clarke L, Singh B, Chepko G, Clarke R, Sholler PF, Lirio AA, Foss C, Reiter R, Trock B, Paik S& Martin MB (2003) Cadmium mimics the in vivo effects of estrogen in the uterus and mammary gland. Nat. Med. 9:1081-1084 https://doi.org/10.1038/nm902.

      [19] Kalender Y, KayaS, Durak D, Uzun F, G. &DemirF (2012) Protective effects of catechin and quercetin on antioxidant status, lipid peroxidation and testis-histoarchitecture induced by chlorpyrifos in male rats. Environ ToxicolPharmacol. Environ ToxicolPharmacol 33:141-148 https://doi.org/10.1016/j.etap.2011.12.008.

      [20] Kara H, Karatas F&Canatan H SK (2005) Effects of exogenous metallothionein on acute cadmium toxicity in rats. . Biol Trace Elem Res 104:223-223-232 https://doi.org/10.1385/BTER:104:3:223.

      [21] KenslerTW, Wakabayashi N, Biswal S. (2007) Cell survival responses to environmental stressesvia the Keap1-Nrf2-ARE pathway,â€. Annual Review of Pharmacology and Toxicology, 47:89-116 https://doi.org/10.1146/annurev.pharmtox.46.120604.141046.

      [22] Khan MS, M. S, Khan MM, A.K.N. K& R.A. A (2003) Cinnamon zeylanicum Statistical Methods. 7th Edition, Iowa State improves glucose and lipids of people with type 2 University Press, Ames, USA, :90-92

      [23] KimSH &ChoungSY (2010) Antihyperglycemic and antihyperlipidemic action of CinnamomiCassiae (Cinnamon bark) extract in C57BL/Ks db/db mice. . Arch Pharm Res 33:325-333 https://doi.org/10.1007/s12272-010-0219-0.

      [24] Kim SH, HyunSH&ChoungSY (2006) Anti-diabetic effect of cinnamon extract on blood glucose in db/db mice. J Ethnopharmacol 104:119-123 https://doi.org/10.1016/j.jep.2005.08.059.

      [25] Livak KJ &Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitive PCR and the 2 (Delta C (T). Method "Methods, 25(4):402-408 https://doi.org/10.1006/meth.2001.1262.

      [26] Nguyen T, Yang CS&B. PC (2004) The pathways and molecular mechanisms regulating Nrf2 activation in response to chemical stress,â€Free Radical Biology and Medicine 37(4):433-441 https://doi.org/10.1016/j.freeradbiomed.2004.04.033.

      [27] ObianimeA&Roberts I (2009) Antioxidants, cadmium induced toxicity, serum biochemical and the histological abnormalities of the kidney and testes of the male Wistar rats. . Niger J PhysiolSci 24:177-185

      [28] Oguzturk H, Ciftci O, Aydin M, Timurkaan N, BeyturA&Yilmaz F (2012) Ameliorative effects of curcumin against acute cadmium toxicity on male reproductive system in rats. . Andrologia 44:243-249 https://doi.org/10.1111/j.1439-0272.2012.01273.x.

      [29] Okamura A, Kamijima M, Shibata E, Ohtani K., Takagi K., Ueyama J., Watanabe Y., Omura M., Wang H., Ichihara G.&T. K, T. N (2005) A comprehensive evaluation of the testicular toxicity of dichlorvosinWistar rats,. Toxicology and Applied Pharmacology, 213(129-137)

      [30] Pandya C, Pillai P, Nampoothiri LP& Bhatt N, S G (2012) Effect of lead and cadmium co-exposure on testicular steroid metabolism and antioxidant system of adult male rats. Andrologia 44:813-822 https://doi.org/10.1111/j.1439-0272.2010.01137.x.

      [31] ParivziN &Ellendorff F (1982) Further evidence on dual effects of norepin-phrine on LH secretion. Neuro. Endocrinol. 35:48-55

      [32] Rubiolo JA, G. M & V. VF (2008) Resveratrol the Nrf2 transcription factor and augmented activities of antioxidant enzyme, European Journal of Pharmacology 591:66-72 https://doi.org/10.1016/j.ejphar.2008.06.067.

      [33] SataY&Tsukanmamoto T (2000) Effects of nitric oxide stimulation on the brain. Drugs Today 36:38 https://doi.org/10.1358/dot.2000.36.2-3.568781.

      [34] Sen Gupta R, Sen Gupta E, DhakalBK&Thakur AR, J A (2004) Vitamin C and vitamin E protect the rat testes from cadmium–induced reactive oxygen species. Mol Cells 17:132-139

      [35] ShagauoRB &Davidson AM (2006) The effect of Cinnamomumzeylanicumon histological structure of testis in rats. Endocrinology 63:241-252

      [36] Shah A, Al-ShareefA&Ageel A, S. Q (1998) Toxicity studies in mice of common spices, Cinnamomumzeylani¬cum bark and Piper longum fruits. Plant Foods for Human Nutrition (Formerly QualitasPlantarum) 52(3):231-239 https://doi.org/10.1023/A:1008088323164.

      [37] ShengX, ZhangY, Gong Z, Huang C&ZangY (2008) Improved Insulin Resistance and Lipid Metabolism by Cinnamon Extract through Activation of Peroxisome Proliferator-Activated Receptors. PPAR Res 2008:581348.https://doi.org/10.1155/2008/581348.

      [38] SimmonsSO, FanCY, YeomanK, Wakefield. J&Ramabhadran R. (2011) Nrf2 oxidative stress induced by heavy metals is cell type dependent, Current Chemical Genomics, 5(1):1-12 https://doi.org/10.2174/1875397301105010001.

      [39] Siu ER, Mruk. D.D& Porto. C.S, CCY (2009) Cadmium induced testicular injury. ToxicolApplPharmacol 238:240-249 https://doi.org/10.1016/j.taap.2009.01.028.

      [40] TakiguchiM&Yoshihara S (2006) new aspects of cadmium as endocrine disruptor. Environ Sci 13:107-116

      [41] Thompson J&Bannigan. (2008) Cadmium: toxic effects on the reproductive system and the embryo. . JReprodToxicol 25:304-315 https://doi.org/10.1016/j.reprotox.2008.02.001.

      [42] Valko M, Rhodes CJ, Moncol J, IzakovicM&Mazur M. (2006) Free radicals, metals and antioxidants in oxidative stress induced cancer. Chemico-Biological Interactions 10(1):1-40 https://doi.org/10.1016/j.cbi.2005.12.009.

      [43] Wang Y, Junyi F, Shaoxin H, Chen L&Guangqin F (2013) the chronic effects of Nrf2 and Mrp1 of the testis in the rats. Enviro. Toxic. andPharmaco 35:109-116 https://doi.org/10.1016/j.etap.2012.12.001.

      [44] Who (1992) Cadmium – environmental aspects. In: Environmental Health Criteria: 135. Dobson S (ed.). World Health Organization, Geneva, Switzerland:156

      [45] Yang HS,DKH, Kim JR&Sim. JC (2006) Effect of α tocopherol on cadmium induced - toxicity in rat testis and carcinogenesis. Korean Med. J 21:445-451 https://doi.org/10.3346/jkms.2006.21.3.445.

      [46] Yang Z, Yang S, Qian S. Y., Hong J, Kadiiska. M., Tennant. R, Waalkes. M&Liu.J (2007) Cadmium-induced toxicity in rat primary mid-brain neuroglia cultures: role of oxidative stress from Microglia. Toxicological Sciences, 98(2):488-494. https://doi.org/10.1093/toxsci/kfm106.

  • Downloads

  • How to Cite

    Ibrahim, S., & Y.Abo-Kora, S. (2017). Evaluation of the protective effect of Cinnamomum Zeylanicum on cadmium testicular toxicity and Nrf2 gene expression in albino rats. International Journal of Pharmacology and Toxicology, 5(1), 5-10. https://doi.org/10.14419/ijpt.v5i1.7030