Protective effect of cinnamon against cadmium-induced hepatorenal oxidative damage in rats

  • Authors

    • Ahmed Abdeen Assistant professor, Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Toukh 13736, Benha University, Egypt
    • Ahmed Ghonim Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Toukh 13736, Benha University, Egypt
    • Ragab El-Shawarby Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Toukh 13736, Benha University, Egypt
    • Nabila Abdel-Aleem Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Toukh 13736, Benha University, Egypt
    • Elham El-Shewy Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Toukh 13736, Benha University, Egypt
    • Mohamed Abdo
    • Ehab Abdelhiee Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Toukh 13736, Benha University, Egypt
    2017-02-03
    https://doi.org/10.14419/ijpt.v5i1.7119
  • , Antioxidants, Cadmium, Cinnamon, Reactive Oxygen Species.

  • Abstract

    Background: Cadmium (Cd) is a well-known hazardous environmental contaminant. It exerts its toxicity through induction of lipid peroxidation and reduction of cellular antioxidant. Therefore, in this study, we investigated whether cinnamon could protect against Cd toxicity in liver and kidney.

    Materials and methods: Forty male Wister rats (130-135 gm) were divided randomly into 4 groups/ 10 rats each. Control, cinnamon, cadmium, and Cinn+Cd groups received distilled water, cinnamon extract (200 mg/kg b.wt. orally), cadmium chloride (5 mg/kg b.wt. orally), and Cd plus cinnamon, respectively. Blood, liver, and kidney samples were collected after 8 weeks of treatment. Erythrogram, leukogram, liver and kidney functions, and oxidative status (MDA, CAT, and TAC) were determined.

    Results and discussion: Cd-treated animals showed significant increases in serum ALT, AST, creatinine, and urea indicating hepatic and renal damage. Cd-induced oxidative stress was observed by marked decease TAC accompanied by increase in MDA which contributed in liver and kidney dysfunction. Co-treatment of Cd with cinnamon has improved the oxidation profile by increasing the TAC and decreasing the lipid peroxidation. Cinnamon ameliorated the toxic effect of Cd, which observed by improvement of liver and kidney functions.

    Conclusion:  High antioxidants content of cinnamon could protect the liver and kidney from Cd toxicity. 

  • References

    1. [1] Abernethy DR, Destefano AJ, Cecil TL, Zaidi K & Williams RL (2010) Metal impurities in food and drugs. Pharmaceutical Research 27, 750-755. https://doi.org/10.1007/s11095-010-0080-3.

      [2] Aebi H (1984) Methods in Enzymology Journal 105, 121-126. https://doi.org/10.1016/S0076-6879(84)05016-3.

      [3] Albasha MO & Azab EA (2014) Effect of cadmium on the liver and amelioration by aqueous extracts of fenugreek seeds, rosemary, and cinnamon in guinea pigs: Histological and biochemical study. Cell Biology 2, 7-17. https://doi.org/10.11648/j.cb.20140202.11.

      [4] Allen LC (1982) Clinical Chemistry Journal 28: 555.

      [5] Amara S, Douki T, Garrel C., Favier, A., Rhouma K, Sakly M & Abdelmelek H (2011) Effect of static magnetic field and cadmium on oxidative stress and DNA damage in rat cortex brain and hippocampus. Toxicolology Indian Health 27, 99-106. https://doi.org/10.1177/0748233710381887.

      [6] Bagchi D, Bagchi M, Hassoun EA & Stohs SJ (1996) Cadmium-induced excretion of urinary lipid metabolites, DNA damage, glutathione depletion and hepatic lipid peroxidation in Sprague-Dawley rats. Biological Trace Element Research 52, 143-154. https://doi.org/10.1007/BF02789456.

      [7] Banchroft JD, Stevens A, & Turner DR (1996) Theory and practice of histological techniques. Fourth Edition. Churchil Livingstone, New York, London, San Francisco, Tokyo.

      [8] Birn H & Christensen EI (2006) renal albumin absorption in physiology and pathology. Kidney International 69, 440-449. https://doi.org/10.1038/sj.ki.5000141.

      [9] Branka I, Ognjanovic S Z, Pavlovic SD, Maletic RV, Zikic AS, Stajn ZS & Vojislav M P (2001) Protective role of vitamin E on antioxidant defense system and lipid peroxide concentration in the blood of rats acutely exposed to cadmium. Kragujevac Journal of Science 23, 115-126.

      [10] Daniel EO & Michael OE (2014) Histological effects of aqueous extract of cinnamon on the kidney functions of adult Wistar rats. SMU Medical Journal 1, 183-191.

      [11] Demetrious JA, Drewes DA & Gin JB (1974) Enzymes. In Clinical Chemistry-Principles and Techniques 2nd ed. RJ Henary, DC Cannon, JW Winkelman, Eds. Harper & Row, Hagerstown MD, p 927.

      [12] Dhaliwal H, Krishenbaum LA, Randhawa AK & Singal PK (1991) Correlation between antioxidant changes during hypoxia and recovery on oxygenation. American Journal of Physiology 261, H632-H638.

      [13] Doumas BT et al. (1971) Clinica Chimica Acta 31, 87 pp.

      [14] Eidi A, Mortazavi P, Bazargan M & Zaringhalam J (2012) Hepatoprotective activity of cinnamon ethanolic extract against CCl4 induced liver injury in rats. Excli Journal 11, 495-507.

      [15] Eisenwiener HG (1976) Journal of clinical chemistry and clinical biochemistry 14, 261-264.

      [16] Elkomy A, Aboubakr M, Soliman A, Abdeen A, Abdelkader A & Hekal H (2016) Paracetamol induced hepatic toxicity and amelioration by cinnamon in rats. International Journal of Pharmacology and Toxicology 4, 187-190. https://doi.org/10.14419/ijpt.v4i2.6529.

      [17] Gornall A (1949) the Journal of Biological Chemistry177, C 751.

      [18] Hjälm G, Murray E, Crumley G, Harazim W, Lundgren S, Onyango I, Ek B, Larsson M, Juhlin C, Hellman P, Davis H, Akerström G, Rask L, & Morse B (1996) Cloning and sequencing of human gp330, a Ca (2+)-binding receptor with potential intracellular signaling properties. European Journal of Biochemistry 239, 132-137. https://doi.org/10.1111/j.1432-1033.1996.0132u.x.

      [19] Hollis L, Hogstrand C & Wood CM (2001) Tissue specific cadmium accumulation, metallothionein induction, and tissue zinc and copper levels during chronic sublethal cadmium exposure in juvenile rainbow trout. Arch. Environmental Contamination and Toxicology. 41, 468-474. https://doi.org/10.1007/s002440010273.

      [20] Hounkpatin ASY, Edorh P A, Guédénon P, Alimba CG, Ogunkanmi A, Dougnon T V, Boni G, Aissi KA, Montcho S, Loko F, Ouazzani N, Mandi L, Boko M & Creppy EE (2013) Haematological evaluation of Wistar rats exposed to chronic doses of cadmium, mercury and combined cadmium and mercury. African Journal of Biotechnology 12, 3731-3737.

      [21] Horiguchi H, Oguma E, Kayama F (2006) Cadmium and cisplatin damage erythropoietin-producing proximal renal tubular cells. Archives Toxicology 80, 680-686. https://doi.org/10.1007/s00204-006-0093-1.

      [22] Kara H, Karatap F & Canatan H (2005) Effect of single dose cadmium chloride administration on oxidative stress in male and female rats. Turkish Journal of Veterinary and Animal Sciences 29, 37-42.

      [23] Klaassen CD, Liu J & Choudhuri S (1999) Metallothionein: An intracellular protein to protect against cadmium toxicity. Annual Review of Pharmacology and Toxicology 39, 267-294. https://doi.org/10.1146/annurev.pharmtox.39.1.267.

      [24] Knapp JZ, Barber TA & Lieberman A (1996) Liquid and surface born particles (Measurement Handbook). Marcel Dekker, pp.113.

      [25] Koracevic D, Koracevic G, Djordjevic V, Andrejevic S & Cosic V (2001) Method for the measurement of antioxidant activity in human fluids. Journal of Clinical Pathology 54, 356-361. https://doi.org/10.1136/jcp.54.5.356.

      [26] Lakshmi DG, Kumar PR, Baharavi K, Annapurna P, Rajendar P & Pankaj TB (2012) Protective effect of Tribulus terrestris Linn on liver and kidney in cadmium intoxicated rats. Indian Journal of Expermental Biology 50, 141-146.

      [27] Lamfon NA (2014) Cinnamon aqueous extract ameliorates deltamethrin-induced hepatotoxicity in albino rats. Research Journal of Pharmaceutical, Biological and Chemical Sciences 5, 898-905.

      [28] Liu J, Liu Y, Michalska AE, Choo KH & Klaassen CD (1996) Distribution and retention of cadmium in metallothionein I and II null mice. Toxicology and Applied Pharmacology 136, 260-268. https://doi.org/10.1006/taap.1996.0033.

      [29] Massanyi P, Tataruch F, Slamecka J, Toman RC & Jurcik R (2003) Accumulation of lead, cadmium, and mercury in liver and kidney of the brown hare (Lepus europaeus) in relation to the season, age, and sex in the West Slovakian Lowland. Journal of Environmental Science and Health 39, 1299-1309. https://doi.org/10.1081/ESE-120021127.

      [30] Maunsbach AB (1966) Albumin absorption by renal proximal tubule cells. Nature, 212, 546-547. https://doi.org/10.1038/212546a0.

      [31] Mauro P & Renze B (2008) In: Fundamentals of Clinical Chemistry. 6th ed. p325.

      [32] Mbeh UE, Francis CO, Edet OA, Nelson CO, Stella CB & Promise N (2012) Reversal of cadmium induced toxicity following dietary supplementation with garlic, ginger and cabbage in male Wistar rats. Journal of Natural Product and Plant Resources 2, 169-174.

      [33] Morgan AM, El-Ballal SS, El-Bialy BE & EL-Boraie NB (2014) Studies on the potential protective effect of cinnamon against bisphenol A- and octylphenol-induced oxidative stress in male albino rats. Toxicology Reports 1, 92-101. https://doi.org/10.1016/j.toxrep.2014.04.003.

      [34] Moselhy SS & Ali HK (2009) Hepatoprotective effect of cinnamon extracts against carbon tetrachloride induced oxidative stress and liver injury in rats. Biology Research 2, 93-98. https://doi.org/10.4067/s0716-97602009000100009.

      [35] Naik P (2010) Biochemistry. 3rd ed. Jaypee Publishers Ltd, Panama. P138-141, 565. https://doi.org/10.5005/jp/books/11094.

      [36] Newairy AA, El-Sharaky AS, Badreldeen MM, Eweda SM & Sheweita SA (2007) the hepatoprotective effects of selenium against cadmium toxicity in rats. Toxicology 242, 23-30. https://doi.org/10.1016/j.tox.2007.09.001.

      [37] Nigam D, Shukla GS & Agarwal AK (1999) Glutathione depletion and oxidative damage in mitochondria following exposure to cadmium in rat liver and kidney. Toxicology Letters 106, 151-157. https://doi.org/10.1016/S0378-4274(99)00059-4.

      [38] Nishida S, Kikuichi S, Yoshioka S, Tsubaki M, Fujii Y & Matsuda H (2003) Induction of apoptosis in HL-60 cells treated with medicinal herbs. The American Journal of Chinese Medicine 31, 551-562. https://doi.org/10.1142/S0192415X03001211.

      [39] Radosavljevici T, Mladenovici D, Ninkovic M, Vucevici D, Boricic I, Jesic-Vukicevic R, Å ljivancanini T, Lopicic S & Todorovic V (2012) Oxidative stress in rat liver during acute cadmium and ethanol intoxication. Journal of Serbian Chemical Society 77, 159-176. https://doi.org/10.2298/JSC110330174R.

      [40] Rikans LE & Yamano T (2000) Mechanisms of cadmium-mediated acute hepatotoxicity. Journal of Biochemical and Molecular Toxicology 14, 110-117. https://doi.org/10.1002/(SICI)1099-0461(2000)14:2<110::AID-JBT7>3.0.CO;2-J.

      [41] Sakr SA & Albarakai AY (2014) Effect of cinnamon on cypermethrin-induced nephrotoxicity in albino rats. International Journal of Advanced Research 2, 578-586.

      [42] Santos FW, Oro T, Zeni G, Rocha JBT, Donascimento PC & Nogueira CW (2004) Cadmium induced testicular damage and its response to administration of succimer and diphenyl diselenide in mice. Toxicology Letters 152, 255-263. https://doi.org/10.1016/j.toxlet.2004.05.009.

      [43] Satoh K (1978) Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica Chemica Acta 90, 37-41. https://doi.org/10.1016/0009-8981(78)90081-5.

      [44] Singh N, Rani P, Gupta M, Goel N & Tandan N (2013) Effects of aqueous extract of Camellia sinensis (l.) O. kuntze on liver markers of cadmium treated rats. Journal of Biotechnology and Pharmaceutical Research 4, 89-93.

      [45] Singh P, Chaudhary S, Patni A & Sankhla V (2007) Effect cadmium chloride induced genotoxicity in bone marrow chromosomes of swiss albino mice and subsequent protective effects of Emblica officinalis and vitamin C. Journal of Herbal Medicine and Toxicology 1, 67-71.

      [46] Stohs SJ, Bagchi D, Hassoun E, & Bagchi M (2000) Oxidative mechanisms in the toxicity of chromium and cadmium ions. Journal of Environmental Pathology, Toxicology and Oncology 20, 77-88.

      [47] Su L, Yin J, Charles D, Zhou K, Moore J & Yu L (2007) Total phenolic contents, chelating capacities, and radical-scavenging properties of black peppercorn, nutmeg, rosehip, cinnamon and oregano leaf. Food Chemistry 100, 990-997. https://doi.org/10.1016/j.foodchem.2005.10.058.

      [48] Takeda T, Yamazaki H & Farquhar MG (2003) Identification of an apical sorting determinant in the cytoplasmic tail of megalin. American Journal of Physiology - Cell Physiology 284, C1105–C1113. https://doi.org/10.1152/ajpcell.00514.2002.

      [49] Tojo A & Kinugasa S (2012) Mechanisms of Glomerular Albumin Filtration and Tubular Reabsorption. International Journal of Nephrology 1-9. https://doi.org/10.1155/2012/481520.

      [50] Toppo R, Roy BK, Gora RH, Baxla SL & Kumar P (2015) Hepatoprotective activity of Moringa oleifera against cadmium toxicity in rats. Veterinary World 8, 537-540. https://doi.org/10.14202/vetworld.2015.537-540.

      [51] Tremellen K (2008) Oxidative stress and male infertility–a clinical perspectives. Human Reproduction Update 14, 243-258. https://doi.org/10.1093/humupd/dmn004.

      [52] Tremellen K (2008) Oxidative stress and male infertility–a clinical perspectives. Human Reproduction Update 14, 243-258. https://doi.org/10.1093/humupd/dmn004.

      [53] Tribowo JA, Arizal MH, Nashrullah M, Aditama AR & Utama DG (2014) Oxidative stress of cadmium-induced ovarian rat toxicity. International Journal of Chemical Engineering and Applications 5, 254-258. https://doi.org/10.7763/IJCEA.2014.V5.389.

      [54] Turner TT and Lysia JJ (2008) Oxidative stress: a common factor in testicular dysfunction. Journal of Andrology 29, 488-498. https://doi.org/10.2164/jandrol.108.005132.

      [55] Yang CH, Li RX & Chuang LY (2012) Antioxidant Activity of Various Parts of Cinnamomum cassia Extracted with Different Extraction Methods. Molecules 17, 7294-7304. https://doi.org/10.3390/molecules17067294.

      [56] Yiin SJ, Chern CL, Sheu JY, Tseng WC & Lin TH (1999) Cadmium-induced renal lipid peroxidation in rats and protection by selenium. Journal of Toxicology and Environmental Health 57, 403-413. https://doi.org/10.1080/009841099157601.

      [57] Z, Klin Chem u, kiln Biochem 8, 1976. 658 and 10, 1972. 182.

      [58] Zahra AJ, Sedigheh T, Alaeyan JN, Jamali H, Alaeyan JM & Alaeyan JA (2014) Histological effects of cinnamon on nephrotoxicity resulted from Gentamicin in rats. Advances in Environmental Biology 8, 11-14.

  • Downloads

  • How to Cite

    Abdeen, A., Ghonim, A., El-Shawarby, R., Abdel-Aleem, N., El-Shewy, E., Abdo, M., & Abdelhiee, E. (2017). Protective effect of cinnamon against cadmium-induced hepatorenal oxidative damage in rats. International Journal of Pharmacology and Toxicology, 5(1), 17-22. https://doi.org/10.14419/ijpt.v5i1.7119

    Received date: 2016-12-17

    Accepted date: 2017-01-11

    Published date: 2017-02-03